Restriction map for
pF1KSDA0069
(Circular) (Six-base) MAPSORT of: pF1KSDA0069.seq Check: 4234 from: 1 to: 3746
pF1KSDA0069 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 270 270
Size: 3746
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AhdI GACnn_n'nnGTC
Cuts at: 176 586 176
Size: 410 3336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 172 1051 2618 172
Size: 879 1567 1300
Fragments arranged by size:
1567 1300 879
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 395 446 750 3099 3355 395
Size: 51 304 2349 256 786
Fragments arranged by size:
2349 786 304 256 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 762 1176 1424 1459 762
Size: 414 248 35 3049
Fragments arranged by size:
3049 414 248 35
BanII G_rGCy'C
Cuts at: 760 1790 760
Size: 1030 2716
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 1371 1464 2520 1371
Size: 93 1056 2597
Fragments arranged by size:
2597 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 738 1099 738
Size: 361 3385
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1171 1929 2506 3002 1171
Size: 758 577 496 1915
Fragments arranged by size:
1915 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 760 1538 1728 2520 760
Size: 778 190 792 1986
Fragments arranged by size:
1986 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 760 1371 1464 1538 1728 1790 2520 760
Size: 611 93 74 190 62 730 1986
Fragments arranged by size:
1986 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 539 2922 539
Size: 2383 1363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 270 605 796 1010 1312 1693 164
Size: 106 335 191 214 302 381 2217
Fragments arranged by size:
2217 381 335 302 214 191 106
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 172 1123 172
Size: 951 2795
BstBI TT'CG_AA
Cuts at: 110 2107 110
Size: 1997 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 159 159
Size: 3746
BstYI r'GATC_y
Cuts at: 1 312 771 1238 1261 1596 1842 2854 2940
Size: 311 459 467 23 335 246 1012 86
Cuts at: 2940 2952 3298 1
Size: 12 346 449
Fragments arranged by size:
1012 467 459 449 346 335 311 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 683 1331 1505 1896 1923 3268 683
Size: 648 174 391 27 1345 1161
Fragments arranged by size:
1345 1161 648 391 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 508 1769 1979 508
Size: 1261 210 2275
Fragments arranged by size:
2275 1261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 1954 2264 2410 3556 145
Size: 1809 310 146 1146 335
Fragments arranged by size:
1809 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 885 885
Size: 3746
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 1765 552
Size: 1213 2533
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 391 1180 1428 2450 3429 391
Size: 789 248 1022 979 708
Fragments arranged by size:
1022 979 789 708 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 572 604 1588 1618 1620 1650 162
Size: 32 378 32 984 30 2 30 2258
Fragments arranged by size:
2258 984 378 32 32 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 685 1507 685
Size: 822 2924
MslI CAynn'nnrTG
Cuts at: 274 544 679 1735 1862 274
Size: 270 135 1056 127 2158
Fragments arranged by size:
2158 1056 270 135 127
MspA1I CmG'CkG
Cuts at: 192 857 1171 1531 2544 2789 192
Size: 665 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 665 360 314 245
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 125 125
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PflMI CCAn_nnn'nTGG
Cuts at: 413 413
Size: 3746
PfoI T'CCnGG_A
Cuts at: 639 639
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PstI C_TGCA'G
Cuts at: 168 793 1478 168
Size: 625 685 2436
Fragments arranged by size:
2436 685 625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 192 1171 1531 192
Size: 979 360 2407
Fragments arranged by size:
2407 979 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 164 789 1474 2467 2658 33
Size: 131 625 685 993 191 1121
Fragments arranged by size:
1121 993 685 625 191 131
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SnaBI TAC'GTA
Cuts at: 738 738
Size: 3746
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 125 880 1234 125
Size: 755 354 2637
Fragments arranged by size:
2637 755 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 564 1329 1462 1921 2130 262
Size: 302 765 133 459 209 1878
Fragments arranged by size:
1878 765 459 302 209 133
TatI w'GTAC_w
Cuts at: 533 671 3261 533
Size: 138 2590 1018
Fragments arranged by size:
2590 1018 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 309 777 309
Size: 468 3278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 1241 273
Size: 968 2778
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI