Restriction map for
pF1KSDA0070
(Circular) (Six-base) MAPSORT of: pF1KSDA0070.seq Check: 3658 from: 1 to: 4930
pF1KSDA0070 4930 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 476 1678 476
Size: 1202 3728
AccI GT'mk_AC
Cuts at: 428 1621 1916 1966 428
Size: 1193 295 50 3392
Fragments arranged by size:
3392 1193 295 50
Acc65I G'GTAC_C
Cuts at: 1944 1944
Size: 4930
AclI AA'CG_TT
Cuts at: 4770 4770
Size: 4930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 406 1394 2753 3185 3934 4202 214
Size: 192 988 1359 432 749 268 942
Fragments arranged by size:
1359 988 942 749 432 268 192
AfeI AGC'GCT
Cuts at: 1579 1579
Size: 4930
AflIII A'CryG_T
Cuts at: 2280 3386 4363 2280
Size: 1106 977 2847
Fragments arranged by size:
2847 1106 977
AgeI A'CCGG_T
Cuts at: 3318 3318
Size: 4930
AlwNI CAG_nnn'CTG
Cuts at: 2233 3802 2233
Size: 1569 3361
ApaLI G'TGCA_C
Cuts at: 3700 3700
Size: 4930
ApoI r'AATT_y
Cuts at: 628 853 1388 1932 4283 4539 628
Size: 225 535 544 2351 256 1019
Fragments arranged by size:
2351 1019 544 535 256 225
AseI AT'TA_AT
Cuts at: 20 641 20
Size: 621 4309
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4930
AvaI C'yCGr_G
Cuts at: 1948 1948
Size: 4930
BamHI G'GATC_C
Cuts at: 518 1953 518
Size: 1435 3495
BanI G'GyrC_C
Cuts at: 1550 1944 2358 2606 2641 1550
Size: 394 414 248 35 3839
Fragments arranged by size:
3839 414 394 248 35
BanII G_rGCy'C
Cuts at: 254 1537 1942 2972 254
Size: 1283 405 1030 2212
Fragments arranged by size:
2212 1283 1030 405
BbeI G_GCGC'C
Cuts at: 2362 2610 2362
Size: 248 4682
BbsI GAAGACnn'nnnn_
Cuts at: 407 1859 407
Size: 1452 3478
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3589 4930 2826
Size: 763 1341 2826
Fragments arranged by size:
2826 1341 763
BclI T'GATC_A
Cuts at: 1515 2448 1515
Size: 933 3997
BglI GCCn_nnn'nGGC
Cuts at: 139 1262 139
Size: 1123 3807
BglII A'GATC_T
Cuts at: 2443 4482 2443
Size: 2039 2891
BlpI GC'TnA_GC
Cuts at: 268 712 2040 268
Size: 444 1328 3158
Fragments arranged by size:
3158 1328 444
Bme1580I G_kGCm'C
Cuts at: 2553 2646 3704 2553
Size: 93 1058 3779
Fragments arranged by size:
3779 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2140 2221 2556 4702 2140
Size: 81 335 2146 2368
Fragments arranged by size:
2368 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1037 3295 1037
Size: 2258 2672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1007 1007
Size: 4930
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2104 2983 3477 3775 1345
Size: 759 879 494 298 2500
Fragments arranged by size:
2500 879 759 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4634 4634
Size: 4930
BsaAI yAC'GTr
Cuts at: 1920 2281 1920
Size: 361 4569
BsaBI GATnn'nnATC
Cuts at: 6 940 2442 4481 6
Size: 934 1502 2039 455
Fragments arranged by size:
2039 1502 934 455
BsaHI Gr'CG_yC
Cuts at: 201 1083 2359 2607 3311 4807 201
Size: 882 1276 248 704 1496 324
Fragments arranged by size:
1496 1276 882 704 324 248
BsaWI w'CCGG_w
Cuts at: 1091 2117 2638 3318 3592 3739 4150 1091
Size: 1026 521 680 274 147 411 1871
Fragments arranged by size:
1871 1026 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 563 593 563
Size: 30 4900
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 584 584
Size: 4930
BseYI C'CCAG_C
Cuts at: 906 1763 2353 3111 3690 4186 906
Size: 857 590 758 579 496 1650
Fragments arranged by size:
1650 857 758 590 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 4930
BsiEI CG_ry'CG
Cuts at: 104 134 146 1065 2470 2516 3726 104
Size: 30 12 919 1405 46 1210 1308
Fragments arranged by size:
1405 1308 1210 919 46 30 12
BsiHKAI G_wGCw'C
Cuts at: 254 1942 2720 2910 3704 254
Size: 1688 778 190 794 1480
Fragments arranged by size:
1688 1480 794 778 190
BsmI GAATG_Cn'
Cuts at: 1429 1429
Size: 4930
BsmBI CGTCTCn'nnnn_
Cuts at: 192 192
Size: 4930
Bsp1286I G_dGCh'C
Cuts at: 254 1537 1942 2553 2646 2720 2910 2972 3704
Size: 1283 405 611 93 74 190 62 732
Cuts at: 3704 254
Size: 1480
Fragments arranged by size:
1480 1283 732 611 405 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1091 4150 1091
Size: 3059 1871
BspHI T'CATG_A
Cuts at: 1595 4106 1595
Size: 2511 2419
BspMI ACCTGCnnnn'nnnn_
Cuts at: 476 502 1678 1978 2192 2494 2875 476
Size: 26 1176 300 214 302 381 2531
Fragments arranged by size:
2531 1176 381 302 300 214 26
BsrBI CCG'CTC
Cuts at: 50 866 1556 3220 3274 4760 4925 50
Size: 816 690 1664 54 1486 165 55
Fragments arranged by size:
1664 1486 816 690 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1620 2840 1620
Size: 1220 3710
BsrFI r'CCGG_y
Cuts at: 2926 3107 3318 2926
Size: 181 211 4538
Fragments arranged by size:
4538 211 181
BssSI C'ACGA_G
Cuts at: 3199 3559 3199
Size: 360 4570
BstAPI GCAn_nnn'nTGC
Cuts at: 2305 2305
Size: 4930
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4930
BstEII G'GTnAC_C
Cuts at: 1236 1236
Size: 4930
BstXI CCAn_nnnn'nTGG
Cuts at: 1252 1252
Size: 4930
BstYI r'GATC_y
Cuts at: 1 518 1953 2420 2443 2778 3024 4038 4124
Size: 517 1435 467 23 335 246 1014 86
Cuts at: 4124 4136 4482 1
Size: 12 346 449
Fragments arranged by size:
1435 1014 517 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1622 1622
Size: 4930
Bsu36I CC'TnA_GG
Cuts at: 323 323
Size: 4930
BtgI C'CryG_G
Cuts at: 125 959 125
Size: 834 4096
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2878 3059 3222 2878
Size: 181 163 4586
Fragments arranged by size:
4586 181 163
BtsI GCAGTG_nn'
Cuts at: 278 736 2171 278
Size: 458 1435 3037
Fragments arranged by size:
3037 1435 458
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4930
DrdI GACnn_nn'nnGTC
Cuts at: 2634 3494 2634
Size: 860 4070
EaeI y'GGCC_r
Cuts at: 131 143 596 962 2513 2687 3078 3105 4452
Size: 12 453 366 1551 174 391 27 1347
Cuts at: 4452 131
Size: 609
Fragments arranged by size:
1551 1347 609 453 391 366 174 27
12
EagI C'GGCC_G
Cuts at: 131 143 2513 131
Size: 12 2370 2548
Fragments arranged by size:
2548 2370 12
EarI CTCTTCn'nnn_
Cuts at: 190 323 1313 2951 3161 190
Size: 133 990 1638 210 1959
Fragments arranged by size:
1959 1638 990 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3136 3448 3594 4740 3136
Size: 312 146 1146 3326
Fragments arranged by size:
3326 1146 312 146
EcoICRI GAG'CTC
Cuts at: 252 1940 252
Size: 1688 3242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 406 1007 1394 2753 3185 3934 4202 214
Size: 192 601 387 1359 432 749 268 942
Fragments arranged by size:
1359 942 749 601 432 387 268 192
EcoO109I rG'GnC_Cy
Cuts at: 319 2067 319
Size: 1748 3182
EcoRI G'AATT_C
Cuts at: 1932 1932
Size: 4930
EcoRV GAT'ATC
Cuts at: 1401 1401
Size: 4930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 570 2947 570
Size: 2377 2553
FspI TGC'GCA
Cuts at: 2709 2709
Size: 4930
HaeII r_GCGC'y
Cuts at: 1581 2362 2610 3634 4613 1581
Size: 781 248 1024 979 1898
Fragments arranged by size:
1898 1024 979 781 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 912 944 1104 1136 2770 2800 2802 2832 912
Size: 32 160 32 1634 30 2 30 3010
Fragments arranged by size:
3010 1634 160 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1281 1967 1281
Size: 686 4244
HindIII A'AGCT_T
Cuts at: 1462 1462
Size: 4930
KasI G'GCGC_C
Cuts at: 2358 2606 2358
Size: 248 4682
KpnI G_GTAC'C
Cuts at: 1948 1948
Size: 4930
MluI A'CGCG_T
Cuts at: 4363 4363
Size: 4930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1832 3601 3785 1832
Size: 1769 184 2977
Fragments arranged by size:
2977 1769 184
MscI TGG'CCA
Cuts at: 598 964 2689 598
Size: 366 1725 2839
Fragments arranged by size:
2839 1725 366
MslI CAynn'nnrTG
Cuts at: 1250 2917 3044 1250
Size: 1667 127 3136
Fragments arranged by size:
3136 1667 127
MspA1I CmG'CkG
Cuts at: 384 1650 1759 2039 2353 2713 3728 3973 384
Size: 1266 109 280 314 360 1015 245 1341
Fragments arranged by size:
1341 1266 1015 360 314 280 245 109
NaeI GCC'GGC
Cuts at: 3109 3109
Size: 4930
NarI GG'CG_CC
Cuts at: 2359 2607 2359
Size: 248 4682
NcoI C'CATG_G
Cuts at: 125 125
Size: 4930
NdeI CA'TA_TG
Cuts at: 758 758
Size: 4930
NgoMIV G'CCGG_C
Cuts at: 3107 3107
Size: 4930
NruI TCG'CGA
Cuts at: 3291 3291
Size: 4930
NspI r_CATG'y
Cuts at: 1981 2481 3390 1981
Size: 500 909 3521
Fragments arranged by size:
3521 909 500
PasI CC'CwG_GG
Cuts at: 949 1335 949
Size: 386 4544
PciI A'CATG_T
Cuts at: 3386 3386
Size: 4930
PfoI T'CCnGG_A
Cuts at: 1478 1478
Size: 4930
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1690 1722 4069 4101 1690
Size: 32 2347 32 2519
Fragments arranged by size:
2519 2347 32 32
PpuMI rG'GwC_Cy
Cuts at: 319 319
Size: 4930
PsiI TTA'TAA
Cuts at: 614 614
Size: 4930
PstI C_TGCA'G
Cuts at: 473 1689 1975 2660 473
Size: 1216 286 685 2743
Fragments arranged by size:
2743 1216 685 286
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4930
PvuII CAG'CTG
Cuts at: 384 1759 2353 2713 384
Size: 1375 594 360 2601
Fragments arranged by size:
2601 1375 594 360
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4930
SacI G_AGCT'C
Cuts at: 254 1942 254
Size: 1688 3242
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4930
SapI GCTCTTCn'nnn_
Cuts at: 1313 2951 3161 1313
Size: 1638 210 3082
Fragments arranged by size:
3082 1638 210
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4930
ScaI AGT'ACT
Cuts at: 1826 4447 1826
Size: 2621 2309
SfcI C'TryA_G
Cuts at: 33 469 1685 1971 2656 3651 3842 33
Size: 436 1216 286 685 995 191 1121
Fragments arranged by size:
1216 1121 995 685 436 286 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1262 1262
Size: 4930
SfoI GGC'GCC
Cuts at: 2360 2608 2360
Size: 248 4682
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4930
SmaI CCC'GGG
Cuts at: 1950 1950
Size: 4930
SmlI C'TyrA_G
Cuts at: 1324 2083 2998 3492 3754 1324
Size: 759 915 494 262 2500
Fragments arranged by size:
2500 915 759 494 262
SnaBI TAC'GTA
Cuts at: 1920 1920
Size: 4930
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4930
SphI G_CATG'C
Cuts at: 1981 2481 1981
Size: 500 4430
StyI C'CwwG_G
Cuts at: 125 1673 1679 2062 2416 125
Size: 1548 6 383 354 2639
Fragments arranged by size:
2639 1548 383 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1262 1535 2511 2644 3103 3314 1262
Size: 273 976 133 459 211 2878
Fragments arranged by size:
2878 976 459 273 211 133
TatI w'GTAC_w
Cuts at: 1726 1824 4445 1726
Size: 98 2621 2211
Fragments arranged by size:
2621 2211 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 404 436 563 595 4447 4479 404
Size: 32 127 32 3852 32 855
Fragments arranged by size:
3852 855 127 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4930
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1884 2423 1884
Size: 539 4391
XmaI C'CCGG_G
Cuts at: 1948 1948
Size: 4930
XmnI GAAnn'nnTTC
Cuts at: 632 1826 632
Size: 1194 3736
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsrGI BssHII ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NheI NotI NsiI PacI PflMI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SgrAI SrfI SspI StuI SwaI XhoI ZraI