Restriction map for
pF1KSDA0074
(Circular) (Six-base) MAPSORT of: pF1KSDA0074.seq Check: 9342 from: 1 to: 5360
pF1KSDA0074 5360 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 653 1145 2398 653
Size: 492 1253 3615
Fragments arranged by size:
3615 1253 492
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 197 1208 2086 3185 3617 4364 4632 158
Size: 39 1011 878 1099 432 747 268 886
Fragments arranged by size:
1099 1011 886 878 747 432 268 39
AfeI AGC'GCT
Cuts at: 2096 2096
Size: 5360
AflII C'TTAA_G
Cuts at: 1717 1717
Size: 5360
AflIII A'CryG_T
Cuts at: 2712 3816 4793 2712
Size: 1104 977 3279
Fragments arranged by size:
3279 1104 977
AgeI A'CCGG_T
Cuts at: 3748 3748
Size: 5360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1067 1067
Size: 5360
AlwNI CAG_nnn'CTG
Cuts at: 1301 2665 4232 1301
Size: 1364 1567 2429
Fragments arranged by size:
2429 1567 1364
ApaLI G'TGCA_C
Cuts at: 4130 4130
Size: 5360
ApoI r'AATT_y
Cuts at: 2021 2364 4713 4969 2021
Size: 343 2349 256 2412
Fragments arranged by size:
2412 2349 343 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5360
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 333 991 2380 333
Size: 658 1389 3313
Fragments arranged by size:
3313 1389 658
BamHI G'GATC_C
Cuts at: 2385 2385
Size: 5360
BanI G'GyrC_C
Cuts at: 249 268 1705 2376 2790 3038 3073 249
Size: 19 1437 671 414 248 35 2536
Fragments arranged by size:
2536 1437 671 414 248 35 19
BanII G_rGCy'C
Cuts at: 1313 2195 2374 3404 1313
Size: 882 179 1030 3269
Fragments arranged by size:
3269 1030 882 179
BbeI G_GCGC'C
Cuts at: 253 2794 3042 253
Size: 2541 248 2571
Fragments arranged by size:
2571 2541 248
BbsI GAAGACnn'nnnn_
Cuts at: 290 871 1775 290
Size: 581 904 3875
Fragments arranged by size:
3875 904 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 219 253 2187 2221 219
Size: 34 1934 34 3358
Fragments arranged by size:
3358 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3258 4019 5360 3258
Size: 761 1341 3258
Fragments arranged by size:
3258 1341 761
BclI T'GATC_A
Cuts at: 2880 2880
Size: 5360
BglII A'GATC_T
Cuts at: 614 1067 2875 4912 614
Size: 453 1808 2037 1062
Fragments arranged by size:
2037 1808 1062 453
BlpI GC'TnA_GC
Cuts at: 1994 2185 2472 1994
Size: 191 287 4882
Fragments arranged by size:
4882 287 191
Bme1580I G_kGCm'C
Cuts at: 1381 2985 3078 4134 1381
Size: 1604 93 1056 2607
Fragments arranged by size:
2607 1604 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 268 2572 2653 2988 5132 268
Size: 2304 81 335 2144 496
Fragments arranged by size:
2304 2144 496 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1006 2337 3727 1006
Size: 1331 1390 2639
Fragments arranged by size:
2639 1390 1331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 1421 1925 375
Size: 1046 504 3810
Fragments arranged by size:
3810 1046 504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2536 3415 3907 4205 2536
Size: 879 492 298 3691
Fragments arranged by size:
3691 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5064 5064
Size: 5360
BsaAI yAC'GTr
Cuts at: 2352 2713 2352
Size: 361 4999
BsaBI GATnn'nnATC
Cuts at: 6 2874 4911 6
Size: 2868 2037 455
Fragments arranged by size:
2868 2037 455
BsaHI Gr'CG_yC
Cuts at: 250 2791 3039 3741 5237 250
Size: 2541 248 702 1496 373
Fragments arranged by size:
2541 1496 702 373 248
BsaWI w'CCGG_w
Cuts at: 2101 2549 3070 3748 4022 4169 4580 2101
Size: 448 521 678 274 147 411 2881
Fragments arranged by size:
2881 678 521 448 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 1783 1813 2336 2366 187
Size: 30 1566 30 523 30 3181
Fragments arranged by size:
3181 1566 523 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 195 2226 195
Size: 2031 3329
BseYI C'CCAG_C
Cuts at: 141 672 2785 3543 4120 4616 141
Size: 531 2113 758 577 496 885
Fragments arranged by size:
2113 885 758 577 531 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 909 1073 909
Size: 164 5196
BsiEI CG_ry'CG
Cuts at: 104 2902 2948 4156 104
Size: 2798 46 1208 1308
Fragments arranged by size:
2798 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2374 3152 3342 4134 2374
Size: 778 190 792 3600
Fragments arranged by size:
3600 792 778 190
BsmI GAATG_Cn'
Cuts at: 510 510
Size: 5360
BsmBI CGTCTCn'nnnn_
Cuts at: 172 172
Size: 5360
Bsp1286I G_dGCh'C
Cuts at: 1313 1381 2195 2374 2985 3078 3152 3342 3404
Size: 68 814 179 611 93 74 190 62
Cuts at: 3404 4134 1313
Size: 730 2539
Fragments arranged by size:
2539 814 730 611 190 179 93 74
68 62
BspEI T'CCGG_A
Cuts at: 2101 4580 2101
Size: 2479 2881
BspHI T'CATG_A
Cuts at: 1284 4536 1284
Size: 3252 2108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2213 2410 2624 2926 3307 2213
Size: 197 214 302 381 4266
Fragments arranged by size:
4266 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 315 3652 3706 5190 5355 50
Size: 265 3337 54 1484 165 55
Fragments arranged by size:
3337 1484 265 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3272 3272
Size: 5360
BsrFI r'CCGG_y
Cuts at: 3358 3539 3748 3358
Size: 181 209 4970
Fragments arranged by size:
4970 209 181
BssSI C'ACGA_G
Cuts at: 3631 3989 3631
Size: 358 5002
BstAPI GCAn_nnn'nTGC
Cuts at: 2737 2737
Size: 5360
BstBI TT'CG_AA
Cuts at: 110 3721 110
Size: 3611 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2049 2253 2049
Size: 204 5156
BstYI r'GATC_y
Cuts at: 1 614 1067 2385 2852 2875 3210 3456 4468
Size: 613 453 1318 467 23 335 246 1012
Cuts at: 4468 4554 4566 4912 1
Size: 86 12 346 449
Fragments arranged by size:
1318 1012 613 467 453 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 654 654
Size: 5360
BtgI C'CryG_G
Cuts at: 125 977 125
Size: 852 4508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 3491 3654 3310
Size: 181 163 5016
Fragments arranged by size:
5016 181 163
BtsI GCAGTG_nn'
Cuts at: 145 921 1054 2603 145
Size: 776 133 1549 2902
Fragments arranged by size:
2902 1549 776 133
DraI TTT'AAA
Cuts at: 579 1031 2359 579
Size: 452 1328 3580
Fragments arranged by size:
3580 1328 452
DrdI GACnn_nn'nnGTC
Cuts at: 1681 3066 3924 1681
Size: 1385 858 3117
Fragments arranged by size:
3117 1385 858
EaeI y'GGCC_r
Cuts at: 1082 2945 3119 3510 3537 4882 1082
Size: 1863 174 391 27 1345 1560
Fragments arranged by size:
1863 1560 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 174 1283 1510 3383 3593 174
Size: 1109 227 1873 210 1941
Fragments arranged by size:
1941 1873 1109 227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3568 3878 4024 5170 3568
Size: 310 146 1146 3758
Fragments arranged by size:
3758 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 5360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 197 375 1208 1421 1925 2086 3185 3617
Size: 39 178 833 213 504 161 1099 432
Cuts at: 3617 4364 4632 158
Size: 747 268 886
Fragments arranged by size:
1099 886 833 747 504 432 268 213
178 161 39
EcoNI CCTnn'n_nnAGG
Cuts at: 393 393
Size: 5360
EcoO109I rG'GnC_Cy
Cuts at: 130 1226 1867 2202 2316 2499 130
Size: 1096 641 335 114 183 2991
Fragments arranged by size:
2991 1096 641 335 183 114
EcoRI G'AATT_C
Cuts at: 2364 2364
Size: 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 795 3379 795
Size: 2584 2776
FspI TGC'GCA
Cuts at: 3141 3141
Size: 5360
HaeII r_GCGC'y
Cuts at: 253 333 1039 1464 2098 2794 3042 4064 5043
Size: 80 706 425 634 696 248 1022 979
Cuts at: 5043 253
Size: 570
Fragments arranged by size:
1022 979 706 696 634 570 425 248
80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 673 705 1117 1149 1946 1978 3202
Size: 32 121 32 412 32 797 32 1224
Cuts at: 3202 3232 3234 3264 520
Size: 30 2 30 2616
Fragments arranged by size:
2616 1224 797 412 121 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1194 1674 2399 1194
Size: 480 725 4155
Fragments arranged by size:
4155 725 480
KasI G'GCGC_C
Cuts at: 249 2790 3038 249
Size: 2541 248 2571
Fragments arranged by size:
2571 2541 248
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1826 4031 4215 1826
Size: 2205 184 2971
Fragments arranged by size:
2971 2205 184
MscI TGG'CCA
Cuts at: 3121 3121
Size: 5360
MslI CAynn'nnrTG
Cuts at: 647 1435 3349 3476 647
Size: 788 1914 127 2531
Fragments arranged by size:
2531 1914 788 127
MspA1I CmG'CkG
Cuts at: 324 2471 2785 3145 4158 4403 324
Size: 2147 314 360 1013 245 1281
Fragments arranged by size:
2147 1281 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3541 3541
Size: 5360
NarI GG'CG_CC
Cuts at: 250 2791 3039 250
Size: 2541 248 2571
Fragments arranged by size:
2571 2541 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5360
NdeI CA'TA_TG
Cuts at: 1971 1971
Size: 5360
NgoMIV G'CCGG_C
Cuts at: 3539 3539
Size: 5360
NspI r_CATG'y
Cuts at: 2079 2413 2913 3820 2079
Size: 334 500 907 3619
Fragments arranged by size:
3619 907 500 334
PasI CC'CwG_GG
Cuts at: 1230 1728 1230
Size: 498 4862
PciI A'CATG_T
Cuts at: 3816 3816
Size: 5360
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 208 240 4499 4531 208
Size: 32 4259 32 1037
Fragments arranged by size:
4259 1037 32 32
PpuMI rG'GwC_Cy
Cuts at: 130 1226 1867 2202 2316 130
Size: 1096 641 335 114 3174
Fragments arranged by size:
3174 1096 641 335 114
PspXI vC'TCGA_Gb
Cuts at: 333 333
Size: 5360
PstI C_TGCA'G
Cuts at: 323 353 2210 2407 3092 323
Size: 30 1857 197 685 2591
Fragments arranged by size:
2591 1857 685 197 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 2785 3145 2785
Size: 360 5000
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 5360
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SanDI GG'GwC_CC
Cuts at: 1226 1226
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 3383 3593 3383
Size: 210 5150
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SexAI A'CCwGG_T
Cuts at: 1166 1708 1166
Size: 542 4818
SfcI C'TryA_G
Cuts at: 33 319 349 933 1652 2206 2403 3088 4081
Size: 286 30 584 719 554 197 685 993
Cuts at: 4081 4272 33
Size: 191 1121
Fragments arranged by size:
1121 993 719 685 584 554 286 197
191 30
SfoI GGC'GCC
Cuts at: 251 2792 3040 251
Size: 2541 248 2571
Fragments arranged by size:
2571 2541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 2382 2382
Size: 5360
SmlI C'TyrA_G
Cuts at: 333 991 1717 2515 3430 3922 4184 333
Size: 658 726 798 915 492 262 1509
Fragments arranged by size:
1509 915 798 726 658 492 262
SnaBI TAC'GTA
Cuts at: 2352 2352
Size: 5360
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 2413 2913 2413
Size: 500 4860
SspI AAT'ATT
Cuts at: 261 1408 261
Size: 1147 4213
StuI AGG'CCT
Cuts at: 1470 1470
Size: 5360
StyI C'CwwG_G
Cuts at: 125 663 1350 1950 2494 2848 125
Size: 538 687 600 544 354 2637
Fragments arranged by size:
2637 687 600 544 538 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2943 3076 3535 3744 2943
Size: 133 459 209 4559
Fragments arranged by size:
4559 459 209 133
TatI w'GTAC_w
Cuts at: 1519 4875 1519
Size: 3356 2004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 187 219 4877 4909 187
Size: 32 4658 32 638
Fragments arranged by size:
4658 638 32 32
Tth111I GACn'n_nGTC
Cuts at: 1431 1608 3157 1431
Size: 177 1549 3634
Fragments arranged by size:
3634 1549 177
XbaI T'CTAG_A
Cuts at: 2391 2391
Size: 5360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 718 1273 1614 2137 2855 579
Size: 139 555 341 523 718 3084
Fragments arranged by size:
3084 718 555 523 341 139
XhoI C'TCGA_G
Cuts at: 333 991 333
Size: 658 4702
XmaI C'CCGG_G
Cuts at: 2380 2380
Size: 5360
XmnI GAAnn'nnTTC
Cuts at: 287 287
Size: 5360
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PsrI SacII SfiI SgrAI SrfI SwaI ZraI