Restriction map for
pF1KSDA0076
(Circular) (Six-base) MAPSORT of: pF1KSDA0076.seq Check: 7912 from: 1 to: 8231
pF1KSDA0076 8231 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3428 3576 3428
Size: 148 8083
AatII G_ACGT'C
Cuts at: 4988 4988
Size: 8231
AccI GT'mk_AC
Cuts at: 1817 4085 5269 1817
Size: 2268 1184 4779
Fragments arranged by size:
4779 2268 1184
Acc65I G'GTAC_C
Cuts at: 5247 5247
Size: 8231
AclI AA'CG_TT
Cuts at: 8071 8071
Size: 8231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1161 1856 2215 3763 4582 6056 6488 7235 7503
Size: 695 359 1548 819 1474 432 747 268
Cuts at: 7503 1161
Size: 1889
Fragments arranged by size:
1889 1548 1474 819 747 695 432 359
268
AfeI AGC'GCT
Cuts at: 2977 2977
Size: 8231
AflIII A'CryG_T
Cuts at: 629 5583 6687 7664 629
Size: 4954 1104 977 1196
Fragments arranged by size:
4954 1196 1104 977
AgeI A'CCGG_T
Cuts at: 6619 6619
Size: 8231
AleI CACnn'nnGTG
Cuts at: 3773 3773
Size: 8231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3212 3212
Size: 8231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 994 1026 2437 2469 3274 3306 4384 4416 5098
Size: 32 1411 32 805 32 1078 32 682
Cuts at: 5098 5130 994
Size: 32 4095
Fragments arranged by size:
4095 1411 1078 805 682 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 779 1995 2142 2307 2439 3342 4047 4101 4361
Size: 1216 147 165 132 903 705 54 260
Cuts at: 4361 4512 5055 5071 5536 7103 779
Size: 151 543 16 465 1567 1907
Fragments arranged by size:
1907 1567 1216 903 705 543 465 260
165 151 147 132 54 16
ApaI G_GGCC'C
Cuts at: 173 398 429 4637 4924 173
Size: 225 31 4208 287 3480
Fragments arranged by size:
4208 3480 287 225 31
ApaLI G'TGCA_C
Cuts at: 1393 7001 1393
Size: 5608 2623
ApoI r'AATT_y
Cuts at: 151 3192 5235 7584 7840 151
Size: 3041 2043 2349 256 542
Fragments arranged by size:
3041 2349 2043 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8231
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8231
AvaI C'yCGr_G
Cuts at: 2215 2817 2834 3521 3692 3966 5165 5251 2215
Size: 602 17 687 171 274 1199 86 5195
Fragments arranged by size:
5195 1199 687 602 274 171 86 17
AvrII C'CTAG_G
Cuts at: 3024 3024
Size: 8231
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4531 4564 4531
Size: 33 8198
BamHI G'GATC_C
Cuts at: 5256 5256
Size: 8231
BanI G'GyrC_C
Cuts at: 159 1027 3447 5247 5661 5909 5944 159
Size: 868 2420 1800 414 248 35 2446
Fragments arranged by size:
2446 2420 1800 868 414 248 35
BanII G_rGCy'C
Cuts at: 173 289 398 429 489 581 954 1093 1144
Size: 116 109 31 60 92 373 139 51
Cuts at: 1144 1151 1500 2617 3440 3511 3834 4604 4637
Size: 7 349 1117 823 71 323 770 33
Cuts at: 4637 4924 5245 6275 173
Size: 287 321 1030 2129
Fragments arranged by size:
2129 1117 1030 823 770 373 349 323
321 287 139 116 109 92 71 60
51 33 31 7
BbeI G_GCGC'C
Cuts at: 5665 5913 5665
Size: 248 7983
BbsI GAAGACnn'nnnn_
Cuts at: 1436 2549 3354 4796 1436
Size: 1113 805 1442 4871
Fragments arranged by size:
4871 1442 1113 805
BbvCI CC'TCA_GC
Cuts at: 1124 2050 4781 1124
Size: 926 2731 4574
Fragments arranged by size:
4574 2731 926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 919 953 2808 2842 3052 3086 3804 3838 919
Size: 34 1855 34 210 34 718 34 5312
Fragments arranged by size:
5312 1855 718 210 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4122 6129 6890 8231 4122
Size: 2007 761 1341 4122
Fragments arranged by size:
4122 2007 1341 761
BclI T'GATC_A
Cuts at: 1802 5751 1802
Size: 3949 4282
BglI GCCn_nnn'nGGC
Cuts at: 168 1509 2260 168
Size: 1341 751 6139
Fragments arranged by size:
6139 1341 751
BglII A'GATC_T
Cuts at: 344 1412 5746 7783 344
Size: 1068 4334 2037 792
Fragments arranged by size:
4334 2037 1068 792
BlpI GC'TnA_GC
Cuts at: 311 556 1092 1166 4487 5343 311
Size: 245 536 74 3321 856 3199
Fragments arranged by size:
3321 3199 856 536 245 74
Bme1580I G_kGCm'C
Cuts at: 164 173 398 429 517 1030 1397 1733 1791
Size: 9 225 31 88 513 367 336 58
Cuts at: 1791 3105 4387 4637 4924 5023 5177 5856 5949
Size: 1314 1282 250 287 99 154 679 93
Cuts at: 5949 7005 164
Size: 1056 1390
Fragments arranged by size:
1390 1314 1282 1056 679 513 367 336
287 250 225 154 99 93 88 58
31 9
BmgBI CAC'GTC
Cuts at: 4083 4472 4083
Size: 389 7842
BmrI ACTGGGnnnn_n'
Cuts at: 5443 5524 5859 8003 5443
Size: 81 335 2144 5671
Fragments arranged by size:
5671 2144 335 81
BmtI G_CTAG'C
Cuts at: 793 793
Size: 8231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2682 4464 4714 6598 2314
Size: 368 1782 250 1884 3947
Fragments arranged by size:
3947 1884 1782 368 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 520 608 681 1021 1027 1429 1536 1648
Size: 54 88 73 340 6 402 107 112
Cuts at: 1648 1657 2284 2443 2601 2950 3132 3364 3525
Size: 9 627 159 158 349 182 232 161
Cuts at: 3525 3997 4012 4078 4144 4924 466
Size: 472 15 66 66 780 3773
Fragments arranged by size:
3773 780 627 472 402 349 340 232
182 161 159 158 112 107 88 73
66 66 54 15 9 6
Bpu10I CC'TnA_GC
Cuts at: 1124 1919 2050 2432 4048 4781 1124
Size: 795 131 382 1616 733 4574
Fragments arranged by size:
4574 1616 795 733 382 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3654 4459 4684 4834 5407 6286 6778 7076 3654
Size: 805 225 150 573 879 492 298 4809
Fragments arranged by size:
4809 879 805 573 492 298 225 150
BsaI GGTCTCn'nnnn_
Cuts at: 3969 4575 7935 3969
Size: 606 3360 4265
Fragments arranged by size:
4265 3360 606
BsaAI yAC'GTr
Cuts at: 552 3771 5223 5584 552
Size: 3219 1452 361 3199
Fragments arranged by size:
3219 3199 1452 361
BsaBI GATnn'nnATC
Cuts at: 6 227 5745 7782 6
Size: 221 5518 2037 455
Fragments arranged by size:
5518 2037 455 221
BsaHI Gr'CG_yC
Cuts at: 4985 5031 5662 5910 6612 8108 4985
Size: 46 631 248 702 1496 5108
Fragments arranged by size:
5108 1496 702 631 248 46
BsaWI w'CCGG_w
Cuts at: 2151 5216 5420 5941 6619 6893 7040 7451 2151
Size: 3065 204 521 678 274 147 411 2931
Fragments arranged by size:
3065 2931 678 521 411 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 507 537 951 981 994 1024 2213 2243 2374
Size: 30 414 30 13 30 1189 30 131
Cuts at: 2374 2404 2626 2656 3274 3304 3493 3523 4384
Size: 30 222 30 618 30 189 30 861
Cuts at: 4384 4414 4975 5005 5098 5128 507
Size: 30 561 30 93 30 3610
Fragments arranged by size:
3610 1189 861 618 561 414 222 189
131 93 30 30 30 30 30 30
30 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 463 517 521 988 1309 1591 1606 3461 4252
Size: 54 4 467 321 282 15 1855 791
Cuts at: 4252 4261 4264 5106 463
Size: 9 3 842 3588
Fragments arranged by size:
3588 1855 842 791 467 321 282 54
15 9 4 3
BseYI C'CCAG_C
Cuts at: 369 703 1061 2017 2370 2529 2645 3251 3384
Size: 334 358 956 353 159 116 606 133
Cuts at: 3384 4028 4201 4476 4481 5656 6414 6991 7487
Size: 644 173 275 5 1175 758 577 496
Cuts at: 7487 369
Size: 1113
Fragments arranged by size:
1175 1113 956 758 644 606 577 496
358 353 334 275 173 159 133 116
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1375 2155 2305 2743 3133 3298 4549 4858
Size: 689 780 150 438 390 165 1251 309
Cuts at: 4858 5172 686
Size: 314 3745
Fragments arranged by size:
3745 1251 780 689 438 390 314 309
165 150
BsiEI CG_ry'CG
Cuts at: 104 5043 5773 5819 7027 104
Size: 4939 730 46 1208 1308
Fragments arranged by size:
4939 1308 1208 730 46
BsiHKAI G_wGCw'C
Cuts at: 317 557 786 875 1093 1397 2300 3177 3511
Size: 240 229 89 218 304 903 877 334
Cuts at: 3511 3650 4054 4178 5245 6023 6213 7005 317
Size: 139 404 124 1067 778 190 792 1543
Fragments arranged by size:
1543 1067 903 877 792 778 404 334
304 240 229 218 190 139 124 89
BsmI GAATG_Cn'
Cuts at: 842 3005 842
Size: 2163 6068
BsmBI CGTCTCn'nnnn_
Cuts at: 2375 3415 3955 4806 5022 2375
Size: 1040 540 851 216 5584
Fragments arranged by size:
5584 1040 851 540 216
Bsp1286I G_dGCh'C
Cuts at: 164 173 289 317 398 429 489 517 557
Size: 9 116 28 81 31 60 28 40
Cuts at: 557 581 786 875 954 1030 1093 1144 1151
Size: 24 205 89 79 76 63 51 7
Cuts at: 1151 1397 1500 1733 1791 2300 2617 3105 3177
Size: 246 103 233 58 509 317 488 72
Cuts at: 3177 3440 3511 3650 3834 4054 4178 4387 4604
Size: 263 71 139 184 220 124 209 217
Cuts at: 4604 4637 4924 5023 5177 5245 5856 5949 6023
Size: 33 287 99 154 68 611 93 74
Cuts at: 6023 6213 6275 7005 164
Size: 190 62 730 1390
Fragments arranged by size:
1390 730 611 509 488 317 287 263
246 233 220 217 209 205 190 184
154 139 124 116 103 99 93 89
81 79 76 74 72 71 68 63
62 60 58 51 40 33 31 28
28 24 9 7
BspEI T'CCGG_A
Cuts at: 2151 7451 2151
Size: 5300 2931
BspHI T'CATG_A
Cuts at: 3542 7407 3542
Size: 3865 4366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1347 3256 3428 3576 4369 4781 5281 5495 5797
Size: 1909 172 148 793 412 500 214 302
Cuts at: 5797 6178 1347
Size: 381 3400
Fragments arranged by size:
3400 1909 793 500 412 381 302 214
172 148
BsrBI CCG'CTC
Cuts at: 50 6523 6577 8061 8226 50
Size: 6473 54 1484 165 55
Fragments arranged by size:
6473 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3871 4286 6143 3871
Size: 415 1857 5959
Fragments arranged by size:
5959 1857 415
BsrFI r'CCGG_y
Cuts at: 2561 2737 2829 5042 6229 6410 6619 2561
Size: 176 92 2213 1187 181 209 4173
Fragments arranged by size:
4173 2213 1187 209 181 176 92
BssSI C'ACGA_G
Cuts at: 2239 4669 6502 6860 2239
Size: 2430 1833 358 3610
Fragments arranged by size:
3610 2430 1833 358
BstAPI GCAn_nnn'nTGC
Cuts at: 321 799 1056 2264 3248 3870 4038 5608 321
Size: 478 257 1208 984 622 168 1570 2944
Fragments arranged by size:
2944 1570 1208 984 622 478 257 168
BstBI TT'CG_AA
Cuts at: 110 6592 110
Size: 6482 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 703 1740 2400 2505 2989 4941 703
Size: 1037 660 105 484 1952 3993
Fragments arranged by size:
3993 1952 1037 660 484 105
BstYI r'GATC_y
Cuts at: 1 242 344 719 1412 1667 1709 2034 2963
Size: 241 102 375 693 255 42 325 929
Cuts at: 2963 3020 3806 5256 5723 5746 6081 6327 7339
Size: 57 786 1450 467 23 335 246 1012
Cuts at: 7339 7425 7437 7783 1
Size: 86 12 346 449
Fragments arranged by size:
1450 1012 929 786 693 467 449 375
346 335 325 255 246 241 102 86
57 42 23 12
Bsu36I CC'TnA_GG
Cuts at: 971 1559 1792 4378 5118 971
Size: 588 233 2586 740 4084
Fragments arranged by size:
4084 2586 740 588 233
BtgI C'CryG_G
Cuts at: 125 1022 2246 2354 3272 3378 3572 4857 125
Size: 897 1224 108 918 106 194 1285 3499
Fragments arranged by size:
3499 1285 1224 918 897 194 108 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 284 6181 6362 6525 284
Size: 5897 181 163 1990
Fragments arranged by size:
5897 1990 181 163
BtsI GCAGTG_nn'
Cuts at: 1252 1478 2221 4220 4471 5189 5474 1252
Size: 226 743 1999 251 718 285 4009
Fragments arranged by size:
4009 1999 743 718 285 251 226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3897 3932 3897
Size: 35 8196
DraI TTT'AAA
Cuts at: 5230 5230
Size: 8231
DrdI GACnn_nn'nnGTC
Cuts at: 607 4888 5937 6795 607
Size: 4281 1049 858 2043
Fragments arranged by size:
4281 2043 1049 858
EaeI y'GGCC_r
Cuts at: 291 381 1019 1475 1724 3046 3464 4794 5816
Size: 90 638 456 249 1322 418 1330 1022
Cuts at: 5816 5990 6381 6408 7753 291
Size: 174 391 27 1345 769
Fragments arranged by size:
1345 1330 1322 1022 769 638 456 418
391 249 174 90 27
EagI C'GGCC_G
Cuts at: 5816 5816
Size: 8231
EarI CTCTTCn'nnn_
Cuts at: 880 1624 2876 3091 4187 4238 4650 4804 6254
Size: 744 1252 215 1096 51 412 154 1450
Cuts at: 6254 6464 880
Size: 210 2647
Fragments arranged by size:
2647 1450 1252 1096 744 412 215 210
154 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 3958 4243 6439 6749 6895 8041 193
Size: 3765 285 2196 310 146 1146 383
Fragments arranged by size:
3765 2196 1146 383 310 285 146
EcoICRI GAG'CTC
Cuts at: 1091 3509 5243 1091
Size: 2418 1734 4079
Fragments arranged by size:
4079 2418 1734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 520 608 681 1021 1027 1161 1429 1536
Size: 54 88 73 340 6 134 268 107
Cuts at: 1536 1648 1657 1856 2215 2284 2443 2601 2950
Size: 112 9 199 359 69 159 158 349
Cuts at: 2950 3132 3364 3525 3763 3997 4012 4078 4144
Size: 182 232 161 238 234 15 66 66
Cuts at: 4144 4582 4924 6056 6488 7235 7503 466
Size: 438 342 1132 432 747 268 1194
Fragments arranged by size:
1194 1132 747 438 432 359 349 342
340 268 268 238 234 232 199 182
161 159 158 134 112 107 88 73
69 66 66 54 15 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1176 1179 2608 4377 4957 5014 1176
Size: 3 1429 1769 580 57 4393
Fragments arranged by size:
4393 1769 1429 580 57 3
EcoO109I rG'GnC_Cy
Cuts at: 169 395 425 426 600 609 707 858 1797
Size: 226 30 1 174 9 98 151 939
Cuts at: 1797 2063 2165 2397 3028 3258 3525 4260 4634
Size: 266 102 232 631 230 267 735 374
Cuts at: 4634 4654 4695 4920 5122 5370 169
Size: 20 41 225 202 248 3030
Fragments arranged by size:
3030 939 735 631 374 267 266 248
232 230 226 225 202 174 151 102
98 41 30 20 9 1
EcoRI G'AATT_C
Cuts at: 151 5235 151
Size: 5084 3147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6250 6250
Size: 8231
FspI TGC'GCA
Cuts at: 6012 6012
Size: 8231
HaeII r_GCGC'y
Cuts at: 563 1515 2738 2979 3715 4074 5665 5913 6935
Size: 952 1223 241 736 359 1591 248 1022
Cuts at: 6935 7914 563
Size: 979 880
Fragments arranged by size:
1591 1223 1022 979 952 880 736 359
248 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 454 486 699 731 1057 1089 2372
Size: 32 151 32 213 32 326 32 1283
Cuts at: 2372 2374 2404 2406 2501 2533 3493 3525 4803
Size: 2 30 2 95 32 960 32 1278
Cuts at: 4803 4835 4975 5007 6073 6103 6105 6135 271
Size: 32 140 32 1066 30 2 30 2367
Fragments arranged by size:
2367 1283 1278 1066 960 326 213 151
140 95 32 32 32 32 32 32
32 32 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 997 1373 5270 997
Size: 376 3897 3958
Fragments arranged by size:
3958 3897 376
KasI G'GCGC_C
Cuts at: 5661 5909 5661
Size: 248 7983
KpnI G_GTAC'C
Cuts at: 5251 5251
Size: 8231
MluI A'CGCG_T
Cuts at: 7664 7664
Size: 8231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2750 4427 6902 7086 2693
Size: 57 1677 2475 184 3838
Fragments arranged by size:
3838 2475 1677 184 57
MscI TGG'CCA
Cuts at: 293 383 1021 1477 3048 3466 5992 293
Size: 90 638 456 1571 418 2526 2532
Fragments arranged by size:
2532 2526 1571 638 456 418 90
MslI CAynn'nnrTG
Cuts at: 628 2521 3773 4862 6220 6347 628
Size: 1893 1252 1089 1358 127 2512
Fragments arranged by size:
2512 1893 1358 1252 1089 127
MspA1I CmG'CkG
Cuts at: 498 779 924 1128 1156 2017 2100 2106 2202
Size: 281 145 204 28 861 83 6 96
Cuts at: 2202 2370 3380 3858 4101 5342 5656 6016 7029
Size: 168 1010 478 243 1241 314 360 1013
Cuts at: 7029 7274 498
Size: 245 1455
Fragments arranged by size:
1455 1241 1013 1010 861 478 360 314
281 245 243 204 168 145 96 83
28 6
NaeI GCC'GGC
Cuts at: 2739 2831 6412 2739
Size: 92 3581 4558
Fragments arranged by size:
4558 3581 92
NarI GG'CG_CC
Cuts at: 5662 5910 5662
Size: 248 7983
NcoI C'CATG_G
Cuts at: 125 1022 4857 125
Size: 897 3835 3499
Fragments arranged by size:
3835 3499 897
NgoMIV G'CCGG_C
Cuts at: 2737 2829 6410 2737
Size: 92 3581 4558
Fragments arranged by size:
4558 3581 92
NheI G'CTAG_C
Cuts at: 789 789
Size: 8231
NspI r_CATG'y
Cuts at: 184 633 904 1407 2473 2766 2829 3433 4519
Size: 449 271 503 1066 293 63 604 1086
Cuts at: 4519 4617 4973 5284 5784 6691 184
Size: 98 356 311 500 907 1724
Fragments arranged by size:
1724 1086 1066 907 604 503 500 449
356 311 293 271 98 63
PasI CC'CwG_GG
Cuts at: 165 1144 3318 4934 165
Size: 979 2174 1616 3462
Fragments arranged by size:
3462 2174 1616 979
PciI A'CATG_T
Cuts at: 629 6687 629
Size: 6058 2173
PflMI CCAn_nnn'nTGG
Cuts at: 213 1061 3044 3123 3237 3342 3840 4101 4803
Size: 848 1983 79 114 105 498 261 702
Cuts at: 4803 213
Size: 3641
Fragments arranged by size:
3641 1983 848 702 498 261 114 105
79
PfoI T'CCnGG_A
Cuts at: 611 914 1673 2030 2300 2927 3056 611
Size: 303 759 357 270 627 129 5786
Fragments arranged by size:
5786 759 627 357 303 270 129
PmeI GTTT'AAAC
Cuts at: 5230 5230
Size: 8231
PmlI CAC'GTG
Cuts at: 552 3771 552
Size: 3219 5012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 994 1026 3274 3306 3658 3690 4384 4416 5098
Size: 32 2248 32 352 32 694 32 682
Cuts at: 5098 5130 7370 7402 994
Size: 32 2240 32 1823
Fragments arranged by size:
2248 2240 1823 694 682 352 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 600 609 707 858 1797 2063 2165 4260 4695
Size: 9 98 151 939 266 102 2095 435
Cuts at: 4695 600
Size: 4136
Fragments arranged by size:
4136 2095 939 435 266 151 102 98
9
PspOMI G'GGCC_C
Cuts at: 169 394 425 4633 4920 169
Size: 225 31 4208 287 3480
Fragments arranged by size:
4208 3480 287 225 31
PspXI vC'TCGA_Gb
Cuts at: 2817 2817
Size: 8231
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1843 1875 1843
Size: 32 8199
PstI C_TGCA'G
Cuts at: 414 1259 1991 2099 2105 3665 3863 4070 4466
Size: 845 732 108 6 1560 198 207 396
Cuts at: 4466 4496 5278 5963 414
Size: 30 782 685 2682
Fragments arranged by size:
2682 1560 845 782 732 685 396 207
198 108 30 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8231
PvuII CAG'CTG
Cuts at: 498 924 1128 2017 2100 2202 2370 3858 4101
Size: 426 204 889 83 102 168 1488 243
Cuts at: 4101 5656 6016 498
Size: 1555 360 2713
Fragments arranged by size:
2713 1555 1488 889 426 360 243 204
168 102 83
RsrII CG'GwC_CG
Cuts at: 3945 6426 3945
Size: 2481 5750
SacI G_AGCT'C
Cuts at: 1093 3511 5245 1093
Size: 2418 1734 4079
Fragments arranged by size:
4079 2418 1734
SacII CC_GC'GG
Cuts at: 3381 3381
Size: 8231
SalI G'TCGA_C
Cuts at: 5268 5268
Size: 8231
SanDI GG'GwC_CC
Cuts at: 707 2063 707
Size: 1356 6875
SapI GCTCTTCn'nnn_
Cuts at: 880 6254 6464 880
Size: 5374 210 2647
Fragments arranged by size:
5374 2647 210
SbfI CC_TGCA'GG
Cuts at: 5278 5278
Size: 8231
ScaI AGT'ACT
Cuts at: 7748 7748
Size: 8231
SfcI C'TryA_G
Cuts at: 33 144 410 1255 1987 2095 2101 3661 3859
Size: 111 266 845 732 108 6 1560 198
Cuts at: 3859 4066 4462 4492 5152 5274 5959 6952 7143
Size: 207 396 30 660 122 685 993 191
Cuts at: 7143 33
Size: 1121
Fragments arranged by size:
1560 1121 993 845 732 685 660 396
266 207 198 191 122 111 108 30
6
SfoI GGC'GCC
Cuts at: 5663 5911 5663
Size: 248 7983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8231
SmaI CCC'GGG
Cuts at: 3523 5167 5253 3523
Size: 1644 86 6501
Fragments arranged by size:
6501 1644 86
SmlI C'TyrA_G
Cuts at: 2817 3669 3966 4438 4699 4849 5386 6301 6793
Size: 852 297 472 261 150 537 915 492
Cuts at: 6793 7055 2817
Size: 262 3993
Fragments arranged by size:
3993 915 852 537 492 472 297 262
261 150
SnaBI TAC'GTA
Cuts at: 5223 5223
Size: 8231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8231
SphI G_CATG'C
Cuts at: 2473 4973 5284 5784 2473
Size: 2500 311 500 4920
Fragments arranged by size:
4920 2500 500 311
SspI AAT'ATT
Cuts at: 3657 3657
Size: 8231
StuI AGG'CCT
Cuts at: 1072 3109 1072
Size: 2037 6194
StyI C'CwwG_G
Cuts at: 125 335 604 1022 1084 1464 2393 3024 3512
Size: 210 269 418 62 380 929 631 488
Cuts at: 3512 4155 4857 4953 5365 5719 125
Size: 643 702 96 412 354 2637
Fragments arranged by size:
2637 929 702 643 631 488 418 412
380 354 269 210 96 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 3991 5212 5814 5947 6406 6615 1380
Size: 2611 1221 602 133 459 209 2996
Fragments arranged by size:
2996 2611 1221 602 459 209 133
TatI w'GTAC_w
Cuts at: 7746 7746
Size: 8231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 507 539 951 983 2213 2245 2626 2658 5083
Size: 32 412 32 1230 32 381 32 2425
Cuts at: 5083 5115 7748 7780 507
Size: 32 2633 32 958
Fragments arranged by size:
2633 2425 1230 958 412 381 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6028 6028
Size: 8231
XbaI T'CTAG_A
Cuts at: 1872 2037 3160 5262 1872
Size: 165 1123 2102 4841
Fragments arranged by size:
4841 2102 1123 165
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1485 5726 1485
Size: 4241 3990
XhoI C'TCGA_G
Cuts at: 2817 3966 2817
Size: 1149 7082
XmaI C'CCGG_G
Cuts at: 3521 5165 5251 3521
Size: 1644 86 6501
Fragments arranged by size:
6501 1644 86
ZraI GAC'GTC
Cuts at: 4986 4986
Size: 8231
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI BfrBI BsiWI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PshAI PsiI
SexAI SfiI SgrAI SrfI SwaI XmnI