Restriction map for
pF1KSDA0084
(Circular) (Six-base) MAPSORT of: pF1KSDA0084.seq Check: 7164 from: 1 to: 4871
pF1KSDA0084 4871 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 661 1425 1909 661
Size: 764 484 3623
Fragments arranged by size:
3623 764 484
Acc65I G'GTAC_C
Cuts at: 1887 1887
Size: 4871
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2696 3128 3875 4143 1396
Size: 1300 432 747 268 2124
Fragments arranged by size:
2124 1300 747 432 268
AflIII A'CryG_T
Cuts at: 716 2223 3327 4304 716
Size: 1507 1104 977 1283
Fragments arranged by size:
1507 1283 1104 977
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AleI CACnn'nnGTG
Cuts at: 1342 1342
Size: 4871
AlwNI CAG_nnn'CTG
Cuts at: 333 591 2176 3743 333
Size: 258 1585 1567 1461
Fragments arranged by size:
1585 1567 1461 258
ApaI G_GGCC'C
Cuts at: 1657 1764 1657
Size: 107 4764
ApaLI G'TGCA_C
Cuts at: 3641 3641
Size: 4871
ApoI r'AATT_y
Cuts at: 595 1875 4224 4480 595
Size: 1280 2349 256 986
Fragments arranged by size:
2349 1280 986 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 1891 1891
Size: 4871
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 601 634 601
Size: 33 4838
BamHI G'GATC_C
Cuts at: 1896 1896
Size: 4871
BanI G'GyrC_C
Cuts at: 816 1887 2301 2549 2584 816
Size: 1071 414 248 35 3103
Fragments arranged by size:
3103 1071 414 248 35
BanII G_rGCy'C
Cuts at: 278 794 1657 1764 1885 2915 278
Size: 516 863 107 121 1030 2234
Fragments arranged by size:
2234 1030 863 516 121 107
BbeI G_GCGC'C
Cuts at: 2305 2553 2305
Size: 248 4623
BbsI GAAGACnn'nnnn_
Cuts at: 412 1125 1571 1577 412
Size: 713 446 6 3706
Fragments arranged by size:
3706 713 446 6
BbvCI CC'TCA_GC
Cuts at: 288 1823 288
Size: 1535 3336
BciVI GTATCCnnnnn_n'
Cuts at: 1398 2769 3530 4871 1398
Size: 1371 761 1341 1398
Fragments arranged by size:
1398 1371 1341 761
BclI T'GATC_A
Cuts at: 440 2391 440
Size: 1951 2920
BglII A'GATC_T
Cuts at: 457 959 2386 4423 457
Size: 502 1427 2037 905
Fragments arranged by size:
2037 1427 905 502
BlpI GC'TnA_GC
Cuts at: 1983 1983
Size: 4871
Bme1580I G_kGCm'C
Cuts at: 821 1301 1657 1764 1787 2496 2589 3645 821
Size: 480 356 107 23 709 93 1056 2047
Fragments arranged by size:
2047 1056 709 480 356 107 93 23
BmrI ACTGGGnnnn_n'
Cuts at: 755 1790 2083 2164 2499 4643 755
Size: 1035 293 81 335 2144 983
Fragments arranged by size:
2144 1035 983 335 293 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 813 960 3238 813
Size: 147 2278 2446
Fragments arranged by size:
2446 2278 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 355 436 745 1458 1610 1817 268
Size: 87 81 309 713 152 207 3322
Fragments arranged by size:
3322 713 309 207 152 87 81
Bpu10I CC'TnA_GC
Cuts at: 288 798 1823 288
Size: 510 1025 3336
Fragments arranged by size:
3336 1025 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2926 3418 3716 2047
Size: 879 492 298 3202
Fragments arranged by size:
3202 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1410 4575 1410
Size: 3165 1706
BsaAI yAC'GTr
Cuts at: 706 1296 1863 2224 706
Size: 590 567 361 3353
Fragments arranged by size:
3353 590 567 361
BsaBI GATnn'nnATC
Cuts at: 6 2385 4422 6
Size: 2379 2037 455
Fragments arranged by size:
2379 2037 455
BsaHI Gr'CG_yC
Cuts at: 409 1070 2302 2550 3252 4748 409
Size: 661 1232 248 702 1496 532
Fragments arranged by size:
1496 1232 702 661 532 248
BsaWI w'CCGG_w
Cuts at: 654 847 906 1103 2060 2581 3259 3533 3680
Size: 193 59 197 957 521 678 274 147
Cuts at: 3680 4091 654
Size: 411 1434
Fragments arranged by size:
1434 957 678 521 411 274 197 193
147 59
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1657 1657
Size: 4871
BseYI C'CCAG_C
Cuts at: 326 769 793 835 2296 3054 3631 4127 326
Size: 443 24 42 1461 758 577 496 1070
Fragments arranged by size:
1461 1070 758 577 496 443 42 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 403 777 1303 1753 355
Size: 48 374 526 450 3473
Fragments arranged by size:
3473 526 450 374 48
BsiEI CG_ry'CG
Cuts at: 104 2413 2459 3667 104
Size: 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 278 422 654 765 1088 1885 2663 2853 3645
Size: 144 232 111 323 797 778 190 792
Cuts at: 3645 278
Size: 1504
Fragments arranged by size:
1504 797 792 778 323 232 190 144
111
Bsp1286I G_dGCh'C
Cuts at: 278 422 654 765 794 821 1088 1301 1657
Size: 144 232 111 29 27 267 213 356
Cuts at: 1657 1764 1787 1885 2496 2589 2663 2853 2915
Size: 107 23 98 611 93 74 190 62
Cuts at: 2915 3645 278
Size: 730 1504
Fragments arranged by size:
1504 730 611 356 267 232 213 190
144 111 107 98 93 74 62 29
27 23
BspEI T'CCGG_A
Cuts at: 847 1103 4091 847
Size: 256 2988 1627
Fragments arranged by size:
2988 1627 256
BspHI T'CATG_A
Cuts at: 4047 4047
Size: 4871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 1921 2135 2437 2818 326
Size: 1595 214 302 381 2379
Fragments arranged by size:
2379 1595 381 302 214
BsrBI CCG'CTC
Cuts at: 50 399 1061 3163 3217 4701 4866 50
Size: 349 662 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 662 349 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2783 2783
Size: 4871
BsrFI r'CCGG_y
Cuts at: 1001 2869 3050 3259 1001
Size: 1868 181 209 2613
Fragments arranged by size:
2613 1868 209 181
BssSI C'ACGA_G
Cuts at: 3142 3500 3142
Size: 358 4513
BstAPI GCAn_nnn'nTGC
Cuts at: 2248 2248
Size: 4871
BstBI TT'CG_AA
Cuts at: 110 3232 110
Size: 3122 1749
BstYI r'GATC_y
Cuts at: 1 457 566 959 1896 2363 2386 2721 2967
Size: 456 109 393 937 467 23 335 246
Cuts at: 2967 3979 4065 4077 4423 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 937 467 456 449 393 346 335
246 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 203 203
Size: 4871
BtgI C'CryG_G
Cuts at: 125 125
Size: 4871
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2821 3002 3165 2821
Size: 181 163 4527
Fragments arranged by size:
4527 181 163
BtsI GCAGTG_nn'
Cuts at: 299 654 765 2114 299
Size: 355 111 1349 3056
Fragments arranged by size:
3056 1349 355 111
DraI TTT'AAA
Cuts at: 1870 1870
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 2577 3435 2577
Size: 858 4013
EaeI y'GGCC_r
Cuts at: 1047 2456 2630 3021 3048 4393 1047
Size: 1409 174 391 27 1345 1525
Fragments arranged by size:
1525 1409 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2456 2456
Size: 4871
EarI CTCTTCn'nnn_
Cuts at: 190 929 979 2894 3104 190
Size: 739 50 1915 210 1957
Fragments arranged by size:
1957 1915 739 210 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3079 3389 3535 4681 3079
Size: 310 146 1146 3269
Fragments arranged by size:
3269 1146 310 146
EcoICRI GAG'CTC
Cuts at: 276 1883 276
Size: 1607 3264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 355 436 745 1396 1458 1610 1817 2696
Size: 87 81 309 651 62 152 207 879
Cuts at: 2696 3128 3875 4143 268
Size: 432 747 268 996
Fragments arranged by size:
996 879 747 651 432 309 268 207
152 87 81 62
EcoO109I rG'GnC_Cy
Cuts at: 284 588 802 1653 1654 1760 1761 2010 284
Size: 304 214 851 1 106 1 249 3145
Fragments arranged by size:
3145 851 304 249 214 106 1 1
EcoRI G'AATT_C
Cuts at: 1875 1875
Size: 4871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4871
FspI TGC'GCA
Cuts at: 2652 2652
Size: 4871
HaeII r_GCGC'y
Cuts at: 2305 2553 3575 4554 2305
Size: 248 1022 979 2622
Fragments arranged by size:
2622 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1172 1204 1407 1439 2713 2743 2745
Size: 32 437 32 203 32 1274 30 2
Cuts at: 2745 2775 703
Size: 30 2799
Fragments arranged by size:
2799 1274 437 203 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1910 1910
Size: 4871
KasI G'GCGC_C
Cuts at: 2301 2549 2301
Size: 248 4623
KpnI G_GTAC'C
Cuts at: 1891 1891
Size: 4871
MluI A'CGCG_T
Cuts at: 716 4304 716
Size: 3588 1283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3542 3726 3542
Size: 184 4687
MscI TGG'CCA
Cuts at: 1049 2632 1049
Size: 1583 3288
MslI CAynn'nnrTG
Cuts at: 1120 1342 2860 2987 1120
Size: 222 1518 127 3004
Fragments arranged by size:
3004 1518 222 127
MspA1I CmG'CkG
Cuts at: 1683 1982 2296 2656 3669 3914 1683
Size: 299 314 360 1013 245 2640
Fragments arranged by size:
2640 1013 360 314 299 245
NaeI GCC'GGC
Cuts at: 1003 3052 1003
Size: 2049 2822
NarI GG'CG_CC
Cuts at: 2302 2550 2302
Size: 248 4623
NcoI C'CATG_G
Cuts at: 125 125
Size: 4871
NgoMIV G'CCGG_C
Cuts at: 1001 3050 1001
Size: 2049 2822
NspI r_CATG'y
Cuts at: 1119 1924 2424 3331 1119
Size: 805 500 907 2659
Fragments arranged by size:
2659 907 805 500
PasI CC'CwG_GG
Cuts at: 280 280
Size: 4871
PciI A'CATG_T
Cuts at: 3327 3327
Size: 4871
PflMI CCAn_nnn'nTGG
Cuts at: 333 333
Size: 4871
PfoI T'CCnGG_A
Cuts at: 245 332 569 1265 1794 245
Size: 87 237 696 529 3322
Fragments arranged by size:
3322 696 529 237 87
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PmlI CAC'GTG
Cuts at: 706 706
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4839
PpuMI rG'GwC_Cy
Cuts at: 284 284
Size: 4871
PspOMI G'GGCC_C
Cuts at: 1653 1760 1653
Size: 107 4764
PstI C_TGCA'G
Cuts at: 1918 2603 1918
Size: 685 4186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 1683 2296 2656 1683
Size: 613 360 3898
Fragments arranged by size:
3898 613 360
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 278 1885 278
Size: 1607 3264
SalI G'TCGA_C
Cuts at: 1908 1908
Size: 4871
SapI GCTCTTCn'nnn_
Cuts at: 929 979 2894 3104 929
Size: 50 1915 210 2696
Fragments arranged by size:
2696 1915 210 50
SbfI CC_TGCA'GG
Cuts at: 1918 1918
Size: 4871
ScaI AGT'ACT
Cuts at: 4388 4388
Size: 4871
SfcI C'TryA_G
Cuts at: 33 1914 2599 3592 3783 33
Size: 1881 685 993 191 1121
Fragments arranged by size:
1881 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2303 2551 2303
Size: 248 4623
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 1893 1893
Size: 4871
SmlI C'TyrA_G
Cuts at: 2026 2941 3433 3695 2026
Size: 915 492 262 3202
Fragments arranged by size:
3202 915 492 262
SnaBI TAC'GTA
Cuts at: 1863 1863
Size: 4871
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4871
SphI G_CATG'C
Cuts at: 1119 1924 2424 1119
Size: 805 500 3566
Fragments arranged by size:
3566 805 500
SspI AAT'ATT
Cuts at: 180 180
Size: 4871
StuI AGG'CCT
Cuts at: 201 201
Size: 4871
StyI C'CwwG_G
Cuts at: 125 920 1140 2005 2359 125
Size: 795 220 865 354 2637
Fragments arranged by size:
2637 865 795 354 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 1702 2454 2587 3046 3255 406
Size: 1296 752 133 459 209 2022
Fragments arranged by size:
2022 1296 752 459 209 133
TatI w'GTAC_w
Cuts at: 4386 4386
Size: 4871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4388 4420 4388
Size: 32 4839
Tth111I GACn'n_nGTC
Cuts at: 1421 2668 1421
Size: 1247 3624
XbaI T'CTAG_A
Cuts at: 1902 1902
Size: 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2366 2366
Size: 4871
XmaI C'CCGG_G
Cuts at: 1891 1891
Size: 4871
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI XmnI ZraI