Restriction map for
pF1KSDA0085
(Circular) (Six-base) MAPSORT of: pF1KSDA0085.seq Check: 7295 from: 1 to: 3923
pF1KSDA0085 3923 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 734 961 734
Size: 227 3696
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AflIII A'CryG_T
Cuts at: 737 1275 2379 3356 737
Size: 538 1104 977 1304
Fragments arranged by size:
1304 1104 977 538
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 549 1228 2795 549
Size: 679 1567 1677
Fragments arranged by size:
1677 1567 679
ApaI G_GGCC'C
Cuts at: 886 886
Size: 3923
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 943 943
Size: 3923
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 463 886 937 1967 463
Size: 423 51 1030 2419
Fragments arranged by size:
2419 1030 423 51
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbsI GAAGACnn'nnnn_
Cuts at: 874 874
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 212 689 770 1443 212
Size: 477 81 673 2692
Fragments arranged by size:
2692 673 477 81
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 602 886 1548 1641 2697 602
Size: 284 662 93 1056 1828
Fragments arranged by size:
1828 1056 662 284 93
BmrI ACTGGGnnnn_n'
Cuts at: 1135 1216 1551 3695 1135
Size: 81 335 2144 1363
Fragments arranged by size:
2144 1363 335 81
BmtI G_CTAG'C
Cuts at: 515 515
Size: 3923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 802 303
Size: 499 3424
Bpu10I CC'TnA_GC
Cuts at: 358 562 358
Size: 204 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1099 1978 2470 2768 472
Size: 627 879 492 298 1627
Fragments arranged by size:
1627 879 627 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 915 1276 915
Size: 361 3562
BsaBI GATnn'nnATC
Cuts at: 6 688 691 1437 3474 6
Size: 682 3 746 2037 455
Fragments arranged by size:
2037 746 682 455 3
BsaHI Gr'CG_yC
Cuts at: 268 1354 1602 2304 3800 268
Size: 1086 248 702 1496 391
Fragments arranged by size:
1496 1086 702 391 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 301
Size: 30 3893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 507 808 507
Size: 301 3622
BseYI C'CCAG_C
Cuts at: 456 646 885 1348 2106 2683 3179 456
Size: 190 239 463 758 577 496 1200
Fragments arranged by size:
1200 758 577 496 463 239 190
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 937 1715 1905 2697 242
Size: 695 778 190 792 1468
Fragments arranged by size:
1468 792 778 695 190
Bsp1286I G_dGCh'C
Cuts at: 242 463 602 886 937 1548 1641 1715 1905
Size: 221 139 284 51 611 93 74 190
Cuts at: 1905 1967 2697 242
Size: 62 730 1468
Fragments arranged by size:
1468 730 611 284 221 190 139 93
74 62 51
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 215 3099 215
Size: 2884 1039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 166 407 973 1187 1489 1870 166
Size: 241 566 214 302 381 2219
Fragments arranged by size:
2219 566 381 302 241 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 110 2284 110
Size: 2174 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 195 195
Size: 3923
BstYI r'GATC_y
Cuts at: 1 948 1415 1438 1773 2019 3031 3117 3129
Size: 947 467 23 335 246 1012 86 12
Cuts at: 3129 3475 1
Size: 346 449
Fragments arranged by size:
1012 947 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 298 298
Size: 3923
BtgI C'CryG_G
Cuts at: 125 258 125
Size: 133 3790
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 165 687 1873 2054 2217 165
Size: 522 1186 181 163 1871
Fragments arranged by size:
1871 1186 522 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 197 1508 1682 2073 2100 3445 197
Size: 1311 174 391 27 1345 675
Fragments arranged by size:
1345 1311 675 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 349 793 1946 2156 349
Size: 444 1153 210 2116
Fragments arranged by size:
2116 1153 444 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 2131 2441 2587 3733 139
Size: 1992 310 146 1146 329
Fragments arranged by size:
1992 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 802 1748 2180 2927 3195 303
Size: 499 946 432 747 268 1031
Fragments arranged by size:
1031 946 747 499 432 268
EcoO109I rG'GnC_Cy
Cuts at: 882 1062 882
Size: 180 3743
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3923
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 1357 1605 2627 3606 1357
Size: 248 1022 979 1674
Fragments arranged by size:
1674 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 1765 1795 1797 1827 301
Size: 32 1432 30 2 30 2397
Fragments arranged by size:
2397 1432 32 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 199 1684 199
Size: 1485 2438
MslI CAynn'nnrTG
Cuts at: 337 595 682 1912 2039 337
Size: 258 87 1230 127 2221
Fragments arranged by size:
2221 1230 258 127 87
MspA1I CmG'CkG
Cuts at: 546 1034 1348 1708 2721 2966 546
Size: 488 314 360 1013 245 1503
Fragments arranged by size:
1503 1013 488 360 314 245
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 125 258 125
Size: 133 3790
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NheI G'CTAG_C
Cuts at: 511 511
Size: 3923
NspI r_CATG'y
Cuts at: 741 976 1476 2383 741
Size: 235 500 907 2281
Fragments arranged by size:
2281 907 500 235
PciI A'CATG_T
Cuts at: 737 2379 737
Size: 1642 2281
PflMI CCAn_nnn'nTGG
Cuts at: 825 825
Size: 3923
PfoI T'CCnGG_A
Cuts at: 499 499
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PspOMI G'GGCC_C
Cuts at: 882 882
Size: 3923
PstI C_TGCA'G
Cuts at: 545 583 970 1655 545
Size: 38 387 685 2813
Fragments arranged by size:
2813 685 387 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 546 1348 1708 546
Size: 802 360 2761
Fragments arranged by size:
2761 802 360
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 349 1946 2156 349
Size: 1597 210 2116
Fragments arranged by size:
2116 1597 210
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SfcI C'TryA_G
Cuts at: 33 541 579 762 966 1651 2644 2835 33
Size: 508 38 183 204 685 993 191 1121
Fragments arranged by size:
1121 993 685 508 204 191 183 38
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 487 1078 1993 2485 2747 487
Size: 591 915 492 262 1663
Fragments arranged by size:
1663 915 591 492 262
SnaBI TAC'GTA
Cuts at: 915 915
Size: 3923
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StyI C'CwwG_G
Cuts at: 125 258 1057 1411 125
Size: 133 799 354 2637
Fragments arranged by size:
2637 799 354 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
XmnI ZraI