Restriction map for
pF1KSDA0088
(Circular) (Six-base) MAPSORT of: pF1KSDA0088.seq Check: 5919 from: 1 to: 5680
pF1KSDA0088 5680 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2669 2669
Size: 5680
AatII G_ACGT'C
Cuts at: 926 926
Size: 5680
AccI GT'mk_AC
Cuts at: 1235 2716 1235
Size: 1481 4199
Acc65I G'GTAC_C
Cuts at: 1823 2694 1823
Size: 871 4809
AclI AA'CG_TT
Cuts at: 5520 5520
Size: 5680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 2276 2492 3503 3935 4684 4952 658
Size: 1618 216 1011 432 749 268 1386
Fragments arranged by size:
1618 1386 1011 749 432 268 216
AfeI AGC'GCT
Cuts at: 1198 1603 1663 1198
Size: 405 60 5215
Fragments arranged by size:
5215 405 60
AflIII A'CryG_T
Cuts at: 1403 2384 3030 4136 5113 1403
Size: 981 646 1106 977 1970
Fragments arranged by size:
1970 1106 981 977 646
AgeI A'CCGG_T
Cuts at: 4068 4068
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 910 2113 2205 2470 2539 2983 4552 910
Size: 1203 92 265 69 444 1569 2038
Fragments arranged by size:
2038 1569 1203 444 265 92 69
ApaI G_GGCC'C
Cuts at: 384 432 944 384
Size: 48 512 5120
Fragments arranged by size:
5120 512 48
ApaLI G'TGCA_C
Cuts at: 1570 4450 1570
Size: 2880 2800
ApoI r'AATT_y
Cuts at: 2682 5033 5289 2682
Size: 2351 256 3073
Fragments arranged by size:
3073 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5680
AvaI C'yCGr_G
Cuts at: 350 1310 2698 350
Size: 960 1388 3332
Fragments arranged by size:
3332 1388 960
AvrII C'CTAG_G
Cuts at: 385 385
Size: 5680
BamHI G'GATC_C
Cuts at: 608 2703 608
Size: 2095 3585
BanI G'GyrC_C
Cuts at: 1823 2694 3108 3356 3391 1823
Size: 871 414 248 35 4112
Fragments arranged by size:
4112 871 414 248 35
BanII G_rGCy'C
Cuts at: 384 432 868 944 1432 2121 2366 2692 3722
Size: 48 436 76 488 689 245 326 1030
Cuts at: 3722 384
Size: 2342
Fragments arranged by size:
2342 1030 689 488 436 326 245 76
48
BbeI G_GCGC'C
Cuts at: 3112 3360 3112
Size: 248 5432
BbsI GAAGACnn'nnnn_
Cuts at: 835 918 2282 835
Size: 83 1364 4233
Fragments arranged by size:
4233 1364 83
BbvCI CC'TCA_GC
Cuts at: 263 416 1175 263
Size: 153 759 4768
Fragments arranged by size:
4768 759 153
BciVI GTATCCnnnnn_n'
Cuts at: 2242 3576 4339 5680 2242
Size: 1334 763 1341 2242
Fragments arranged by size:
2242 1341 1334 763
BclI T'GATC_A
Cuts at: 1086 2064 3198 1086
Size: 978 1134 3568
Fragments arranged by size:
3568 1134 978
BglI GCCn_nnn'nGGC
Cuts at: 1396 1396
Size: 5680
BglII A'GATC_T
Cuts at: 1715 3193 5232 1715
Size: 1478 2039 2163
Fragments arranged by size:
2163 2039 1478
BlpI GC'TnA_GC
Cuts at: 1350 1431 2460 2790 1350
Size: 81 1029 330 4240
Fragments arranged by size:
4240 1029 330 81
Bme1580I G_kGCm'C
Cuts at: 384 432 944 1574 1860 3303 3396 4454 384
Size: 48 512 630 286 1443 93 1058 1610
Fragments arranged by size:
1610 1443 1058 630 512 286 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 1157 1886 2890 2971 3306 5452 1157
Size: 729 1004 81 335 2146 1385
Fragments arranged by size:
2146 1385 1004 729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4045 4045
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 2135 2439 2533 2549 172
Size: 1963 304 94 16 3303
Fragments arranged by size:
3303 1963 304 94 16
Bpu10I CC'TnA_GC
Cuts at: 263 416 614 1175 263
Size: 153 198 561 4768
Fragments arranged by size:
4768 561 198 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 1489 2339 2854 3733 4227 4525 1213
Size: 276 850 515 879 494 298 2368
Fragments arranged by size:
2368 879 850 515 494 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 396 787 868 2593 5384 396
Size: 391 81 1725 2791 692
Fragments arranged by size:
2791 1725 692 391 81
BsaAI yAC'GTr
Cuts at: 2670 3031 2670
Size: 361 5319
BsaBI GATnn'nnATC
Cuts at: 6 3192 5231 6
Size: 3186 2039 455
Fragments arranged by size:
3186 2039 455
BsaHI Gr'CG_yC
Cuts at: 923 3109 3357 4061 5557 923
Size: 2186 248 704 1496 1046
Fragments arranged by size:
2186 1496 1046 704 248
BsaWI w'CCGG_w
Cuts at: 2867 3388 4068 4342 4489 4900 2867
Size: 521 680 274 147 411 3647
Fragments arranged by size:
3647 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2146 2176 2146
Size: 30 5650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 529 1291 484
Size: 45 762 4873
Fragments arranged by size:
4873 762 45
BseYI C'CCAG_C
Cuts at: 411 431 936 1514 1529 1658 3103 3861 4440
Size: 20 505 578 15 129 1445 758 579
Cuts at: 4440 4936 411
Size: 496 1155
Fragments arranged by size:
1445 1155 758 579 578 505 496 129
20 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2047
Size: 5680
BsiEI CG_ry'CG
Cuts at: 104 3220 3266 4476 104
Size: 3116 46 1210 1308
Fragments arranged by size:
3116 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 740 1538 1574 2366 2692 3470 3660 4454 740
Size: 798 36 792 326 778 190 794 1966
Fragments arranged by size:
1966 798 794 792 778 326 190 36
BsmI GAATG_Cn'
Cuts at: 2000 2000
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 384 432 740 868 944 1432 1538 1574 1860
Size: 48 308 128 76 488 106 36 286
Cuts at: 1860 2121 2366 2692 3303 3396 3470 3660 3722
Size: 261 245 326 611 93 74 190 62
Cuts at: 3722 4454 384
Size: 732 1610
Fragments arranged by size:
1610 732 611 488 326 308 286 261
245 190 128 106 93 76 74 62
48 36
BspEI T'CCGG_A
Cuts at: 4900 4900
Size: 5680
BspHI T'CATG_A
Cuts at: 2009 4856 2009
Size: 2847 2833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 866 1103 2219 2669 2728 2942 3244 3625 866
Size: 237 1116 450 59 214 302 381 2921
Fragments arranged by size:
2921 1116 450 381 302 237 214 59
BsrBI CCG'CTC
Cuts at: 50 2505 3970 4024 5510 5675 50
Size: 2455 1465 54 1486 165 55
Fragments arranged by size:
2455 1486 1465 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3590 3590
Size: 5680
BsrFI r'CCGG_y
Cuts at: 3676 3857 4068 3676
Size: 181 211 5288
Fragments arranged by size:
5288 211 181
BsrGI T'GTAC_A
Cuts at: 701 701
Size: 5680
BssSI C'ACGA_G
Cuts at: 1273 3949 4309 1273
Size: 2676 360 2644
Fragments arranged by size:
2676 2644 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3055 3055
Size: 5680
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1357 1494 1357
Size: 137 5543
BstXI CCAn_nnnn'nTGG
Cuts at: 509 564 1522 1666 509
Size: 55 958 144 4523
Fragments arranged by size:
4523 958 144 55
BstYI r'GATC_y
Cuts at: 1 608 1715 2560 2703 3170 3193 3528 3774
Size: 607 1107 845 143 467 23 335 246
Cuts at: 3774 4788 4874 4886 5232 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1107 1014 845 607 467 449 346 335
246 143 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 437 631 1490 2036 2342 437
Size: 194 859 546 306 3775
Fragments arranged by size:
3775 859 546 306 194
BtgI C'CryG_G
Cuts at: 257 1891 2121 257
Size: 1634 230 3816
Fragments arranged by size:
3816 1634 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 437 742 3628 3809 3972 437
Size: 305 2886 181 163 2145
Fragments arranged by size:
2886 2145 305 181 163
BtsI GCAGTG_nn'
Cuts at: 818 1691 2921 818
Size: 873 1230 3577
Fragments arranged by size:
3577 1230 873
DraI TTT'AAA
Cuts at: 2677 2677
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 3384 4244 3384
Size: 860 4820
EaeI y'GGCC_r
Cuts at: 295 1226 1342 1634 2145 3263 3437 3828 3855
Size: 931 116 292 511 1118 174 391 27
Cuts at: 3855 5202 295
Size: 1347 773
Fragments arranged by size:
1347 1118 931 773 511 391 292 174
116 27
EagI C'GGCC_G
Cuts at: 3263 3263
Size: 5680
EarI CTCTTCn'nnn_
Cuts at: 1012 3701 3911 1012
Size: 2689 210 2781
Fragments arranged by size:
2781 2689 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 1230 1786 3886 4198 4344 5490 292
Size: 938 556 2100 312 146 1146 482
Fragments arranged by size:
2100 1146 938 556 482 312 146
EcoICRI GAG'CTC
Cuts at: 2364 2690 2364
Size: 326 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 658 2135 2276 2439 2492 2533 2549 3503
Size: 486 1477 141 163 53 41 16 954
Cuts at: 3503 3935 4684 4952 172
Size: 432 749 268 900
Fragments arranged by size:
1477 954 900 749 486 432 268 163
141 53 41 16
EcoNI CCTnn'n_nnAGG
Cuts at: 316 2147 316
Size: 1831 3849
EcoO109I rG'GnC_Cy
Cuts at: 268 380 381 428 940 941 1158 1388 1640
Size: 112 1 47 512 1 217 230 252
Cuts at: 1640 2015 2817 268
Size: 375 802 3131
Fragments arranged by size:
3131 802 512 375 252 230 217 112
47 1 1
EcoRI G'AATT_C
Cuts at: 2682 2682
Size: 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 300 3697 300
Size: 3397 2283
FspI TGC'GCA
Cuts at: 2620 3459 2620
Size: 839 4841
HaeII r_GCGC'y
Cuts at: 1200 1605 1665 3112 3360 4384 5363 1200
Size: 405 60 1447 248 1024 979 1517
Fragments arranged by size:
1517 1447 1024 979 405 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2243 2275 3520 3550 3552 3582 2243
Size: 32 1245 30 2 30 4341
Fragments arranged by size:
4341 1245 32 30 30 2
HincII GTy'rAC
Cuts at: 254 2717 254
Size: 2463 3217
HpaI GTT'AAC
Cuts at: 254 254
Size: 5680
KasI G'GCGC_C
Cuts at: 3108 3356 3108
Size: 248 5432
KpnI G_GTAC'C
Cuts at: 1827 2698 1827
Size: 871 4809
MluI A'CGCG_T
Cuts at: 5113 5113
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 839 1015 4351 4535 839
Size: 176 3336 184 1984
Fragments arranged by size:
3336 1984 184 176
MscI TGG'CCA
Cuts at: 1228 1344 1636 2147 3439 1228
Size: 116 292 511 1292 3469
Fragments arranged by size:
3469 1292 511 292 116
MslI CAynn'nnrTG
Cuts at: 562 602 1133 1249 1541 2635 3667 3794 562
Size: 40 531 116 292 1094 1032 127 2448
Fragments arranged by size:
2448 1094 1032 531 292 127 116 40
MspA1I CmG'CkG
Cuts at: 415 1354 2539 2789 3103 3463 4478 4723 415
Size: 939 1185 250 314 360 1015 245 1372
Fragments arranged by size:
1372 1185 1015 939 360 314 250 245
NaeI GCC'GGC
Cuts at: 3859 3859
Size: 5680
NarI GG'CG_CC
Cuts at: 3109 3357 3109
Size: 248 5432
NcoI C'CATG_G
Cuts at: 257 1891 2121 257
Size: 1634 230 3816
Fragments arranged by size:
3816 1634 230
NgoMIV G'CCGG_C
Cuts at: 3857 3857
Size: 5680
NruI TCG'CGA
Cuts at: 761 4041 761
Size: 3280 2400
NspI r_CATG'y
Cuts at: 601 622 1132 1407 1864 2731 3231 4140 601
Size: 21 510 275 457 867 500 909 2141
Fragments arranged by size:
2141 909 867 510 500 457 275 21
PasI CC'CwG_GG
Cuts at: 449 449
Size: 5680
PciI A'CATG_T
Cuts at: 1403 4136 1403
Size: 2733 2947
PmeI GTTT'AAAC
Cuts at: 2677 2677
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 4819 4851 114
Size: 32 4673 32 943
Fragments arranged by size:
4673 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 268 1158 268
Size: 890 4790
PshAI GACnn'nnGTC
Cuts at: 326 1163 326
Size: 837 4843
PspOMI G'GGCC_C
Cuts at: 380 428 940 380
Size: 48 512 5120
Fragments arranged by size:
5120 512 48
PstI C_TGCA'G
Cuts at: 792 863 2725 3410 792
Size: 71 1862 685 3062
Fragments arranged by size:
3062 1862 685 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5680
PvuII CAG'CTG
Cuts at: 415 1354 2539 3103 3463 415
Size: 939 1185 564 360 2632
Fragments arranged by size:
2632 1185 939 564 360
RsrII CG'GwC_CG
Cuts at: 3873 3873
Size: 5680
SacI G_AGCT'C
Cuts at: 2366 2692 2366
Size: 326 5354
SalI G'TCGA_C
Cuts at: 2715 2715
Size: 5680
SanDI GG'GwC_CC
Cuts at: 268 1158 268
Size: 890 4790
SapI GCTCTTCn'nnn_
Cuts at: 3701 3911 3701
Size: 210 5470
SbfI CC_TGCA'GG
Cuts at: 863 2725 863
Size: 1862 3818
ScaI AGT'ACT
Cuts at: 2071 5197 2071
Size: 3126 2554
SfcI C'TryA_G
Cuts at: 33 788 859 2721 3406 4401 4592 33
Size: 755 71 1862 685 995 191 1121
Fragments arranged by size:
1862 1121 995 755 685 191 71
SfoI GGC'GCC
Cuts at: 3110 3358 3110
Size: 248 5432
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5680
SmaI CCC'GGG
Cuts at: 1312 2700 1312
Size: 1388 4292
SmlI C'TyrA_G
Cuts at: 1192 1504 2354 2833 3748 4242 4504 1192
Size: 312 850 479 915 494 262 2368
Fragments arranged by size:
2368 915 850 494 479 312 262
SnaBI TAC'GTA
Cuts at: 2670 2670
Size: 5680
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5680
SphI G_CATG'C
Cuts at: 622 2731 3231 622
Size: 2109 500 3071
Fragments arranged by size:
3071 2109 500
StyI C'CwwG_G
Cuts at: 257 385 1891 1934 2121 2148 2812 3166 257
Size: 128 1506 43 187 27 664 354 2771
Fragments arranged by size:
2771 1506 664 354 187 128 43 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 338 462 1768 1822 2013 3261 3394 3853 4064
Size: 124 1306 54 191 1248 133 459 211
Cuts at: 4064 338
Size: 1954
Fragments arranged by size:
1954 1306 1248 459 211 191 133 124
54
TatI w'GTAC_w
Cuts at: 701 2069 5195 701
Size: 1368 3126 1186
Fragments arranged by size:
3126 1368 1186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 284 316 5197 5229 284
Size: 32 4881 32 735
Fragments arranged by size:
4881 735 32 32
Tth111I GACn'n_nGTC
Cuts at: 3475 3475
Size: 5680
XbaI T'CTAG_A
Cuts at: 2709 2709
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1873 2188 3173 1873
Size: 315 985 4380
Fragments arranged by size:
4380 985 315
XmaI C'CCGG_G
Cuts at: 1310 2698 1310
Size: 1388 4292
XmnI GAAnn'nnTTC
Cuts at: 2000 2000
Size: 5680
ZraI GAC'GTC
Cuts at: 924 924
Size: 5680
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NsiI PacI PflMI PfoI PmlI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI