Restriction map for
pF1KSDA0098
(Circular) (Six-base) MAPSORT of: pF1KSDA0098.seq Check: 7784 from: 1 to: 4762
pF1KSDA0098 4762 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1798 1798
Size: 4762
Acc65I G'GTAC_C
Cuts at: 1776 1776
Size: 4762
AclI AA'CG_TT
Cuts at: 4602 4602
Size: 4762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1054 1763 2585 3017 3766 4034 940
Size: 114 709 822 432 749 268 1668
Fragments arranged by size:
1668 822 749 709 432 268 114
AflII C'TTAA_G
Cuts at: 357 357
Size: 4762
AflIII A'CryG_T
Cuts at: 880 2112 3218 4195 880
Size: 1232 1106 977 1447
Fragments arranged by size:
1447 1232 1106 977
AgeI A'CCGG_T
Cuts at: 3150 3150
Size: 4762
AhdI GACnn_n'nnGTC
Cuts at: 198 198
Size: 4762
AleI CACnn'nnGTG
Cuts at: 448 448
Size: 4762
AlwNI CAG_nnn'CTG
Cuts at: 2065 3634 2065
Size: 1569 3193
ApaLI G'TGCA_C
Cuts at: 3532 3532
Size: 4762
ApoI r'AATT_y
Cuts at: 976 1764 4115 4371 976
Size: 788 2351 256 1367
Fragments arranged by size:
2351 1367 788 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4762
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4762
AvaI C'yCGr_G
Cuts at: 1780 1780
Size: 4762
BamHI G'GATC_C
Cuts at: 1785 1785
Size: 4762
BanI G'GyrC_C
Cuts at: 164 466 1776 2190 2438 2473 164
Size: 302 1310 414 248 35 2453
Fragments arranged by size:
2453 1310 414 302 248 35
BanII G_rGCy'C
Cuts at: 649 1169 1774 2804 649
Size: 520 605 1030 2607
Fragments arranged by size:
2607 1030 605 520
BbeI G_GCGC'C
Cuts at: 168 2194 2442 168
Size: 2026 248 2488
Fragments arranged by size:
2488 2026 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 529 563 529
Size: 34 4728
BciVI GTATCCnnnnn_n'
Cuts at: 2658 3421 4762 2658
Size: 763 1341 2658
Fragments arranged by size:
2658 1341 763
BclI T'GATC_A
Cuts at: 375 1304 2280 375
Size: 929 976 2857
Fragments arranged by size:
2857 976 929
BglII A'GATC_T
Cuts at: 1208 2275 4314 1208
Size: 1067 2039 1656
Fragments arranged by size:
2039 1656 1067
BlpI GC'TnA_GC
Cuts at: 1872 1872
Size: 4762
Bme1580I G_kGCm'C
Cuts at: 1416 1447 2385 2478 3536 1416
Size: 31 938 93 1058 2642
Fragments arranged by size:
2642 1058 938 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 1972 2053 2388 4534 1972
Size: 81 335 2146 2200
Fragments arranged by size:
2200 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 4762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1248 1510 1754 802
Size: 446 262 244 3810
Fragments arranged by size:
3810 446 262 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 238 1936 2815 3309 3607 211
Size: 27 1698 879 494 298 1366
Fragments arranged by size:
1698 1366 879 494 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 4466 4466
Size: 4762
BsaAI yAC'GTr
Cuts at: 1752 2113 1752
Size: 361 4401
BsaBI GATnn'nnATC
Cuts at: 6 374 1126 2274 4313 6
Size: 368 752 1148 2039 455
Fragments arranged by size:
2039 1148 752 455 368
BsaHI Gr'CG_yC
Cuts at: 130 165 2191 2439 3143 4639 130
Size: 35 2026 248 704 1496 253
Fragments arranged by size:
2026 1496 704 253 248 35
BsaWI w'CCGG_w
Cuts at: 1352 1949 2470 3150 3424 3571 3982 1352
Size: 597 521 680 274 147 411 2132
Fragments arranged by size:
2132 680 597 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 440 470 440
Size: 30 4732
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1304 1327 1403 1304
Size: 23 76 4663
Fragments arranged by size:
4663 76 23
BseYI C'CCAG_C
Cuts at: 642 1094 1182 2185 2943 3522 4018 642
Size: 452 88 1003 758 579 496 1386
Fragments arranged by size:
1386 1003 758 579 496 452 88
BsiEI CG_ry'CG
Cuts at: 104 2302 2348 3558 104
Size: 2198 46 1210 1308
Fragments arranged by size:
2198 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1169 1774 2552 2742 3536 1169
Size: 605 778 190 794 2395
Fragments arranged by size:
2395 794 778 605 190
BsmI GAATG_Cn'
Cuts at: 506 702 506
Size: 196 4566
BsmBI CGTCTCn'nnnn_
Cuts at: 725 725
Size: 4762
Bsp1286I G_dGCh'C
Cuts at: 649 1169 1416 1447 1774 2385 2478 2552 2742
Size: 520 247 31 327 611 93 74 190
Cuts at: 2742 2804 3536 649
Size: 62 732 1875
Fragments arranged by size:
1875 732 611 520 327 247 190 93
74 62 31
BspEI T'CCGG_A
Cuts at: 1352 3982 1352
Size: 2630 2132
BspHI T'CATG_A
Cuts at: 3938 3938
Size: 4762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1810 2024 2326 2707 1810
Size: 214 302 381 3865
Fragments arranged by size:
3865 381 302 214
BsrBI CCG'CTC
Cuts at: 50 726 3052 3106 4592 4757 50
Size: 676 2326 54 1486 165 55
Fragments arranged by size:
2326 1486 676 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2672 2672
Size: 4762
BsrFI r'CCGG_y
Cuts at: 2758 2939 3150 2758
Size: 181 211 4370
Fragments arranged by size:
4370 211 181
BsrGI T'GTAC_A
Cuts at: 1200 1200
Size: 4762
BssSI C'ACGA_G
Cuts at: 3031 3391 3031
Size: 360 4402
BstAPI GCAn_nnn'nTGC
Cuts at: 2137 2137
Size: 4762
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4762
BstXI CCAn_nnnn'nTGG
Cuts at: 396 450 396
Size: 54 4708
BstYI r'GATC_y
Cuts at: 1 1208 1785 2252 2275 2610 2856 3870 3956
Size: 1207 577 467 23 335 246 1014 86
Cuts at: 3956 3968 4314 1
Size: 12 346 449
Fragments arranged by size:
1207 1014 577 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 134 1502 125
Size: 9 1368 3385
Fragments arranged by size:
3385 1368 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1113 2710 2891 3054 1113
Size: 1597 181 163 2821
Fragments arranged by size:
2821 1597 181 163
BtsI GCAGTG_nn'
Cuts at: 1258 2003 1258
Size: 745 4017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 833 868 833
Size: 35 4727
DraI TTT'AAA
Cuts at: 1759 1759
Size: 4762
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3326 2466
Size: 860 3902
EaeI y'GGCC_r
Cuts at: 2345 2519 2910 2937 4284 2345
Size: 174 391 27 1347 2823
Fragments arranged by size:
2823 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2345 2345
Size: 4762
EarI CTCTTCn'nnn_
Cuts at: 977 2783 2993 977
Size: 1806 210 2746
Fragments arranged by size:
2746 1806 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1158 1450 2968 3280 3426 4572 1158
Size: 292 1518 312 146 1146 1348
Fragments arranged by size:
1518 1348 1146 312 292 146
EcoICRI GAG'CTC
Cuts at: 1167 1772 1167
Size: 605 4157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 940 1054 1248 1510 1754 1763 2585 3017
Size: 138 114 194 262 244 9 822 432
Cuts at: 3017 3766 4034 802
Size: 749 268 1530
Fragments arranged by size:
1530 822 749 432 268 262 244 194
138 114 9
EcoO109I rG'GnC_Cy
Cuts at: 140 222 1105 1899 140
Size: 82 883 794 3003
Fragments arranged by size:
3003 883 794 82
EcoRI G'AATT_C
Cuts at: 1764 1764
Size: 4762
EcoRV GAT'ATC
Cuts at: 1406 1676 1406
Size: 270 4492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1203 2779 1203
Size: 1576 3186
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4762
HaeII r_GCGC'y
Cuts at: 168 2194 2442 3466 4445 168
Size: 2026 248 1024 979 485
Fragments arranged by size:
2026 1024 979 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 440 472 916 948 1130 1162 2602
Size: 32 224 32 444 32 182 32 1440
Cuts at: 2602 2632 2634 2664 184
Size: 30 2 30 2282
Fragments arranged by size:
2282 1440 444 224 182 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 597 660 738 1799 597
Size: 63 78 1061 3560
Fragments arranged by size:
3560 1061 78 63
KasI G'GCGC_C
Cuts at: 164 2190 2438 164
Size: 2026 248 2488
Fragments arranged by size:
2488 2026 248
KpnI G_GTAC'C
Cuts at: 1780 1780
Size: 4762
MfeI C'AATT_G
Cuts at: 490 490
Size: 4762
MluI A'CGCG_T
Cuts at: 4195 4195
Size: 4762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3433 3617 3433
Size: 184 4578
MscI TGG'CCA
Cuts at: 2521 2521
Size: 4762
MslI CAynn'nnrTG
Cuts at: 448 1501 2749 2876 448
Size: 1053 1248 127 2334
Fragments arranged by size:
2334 1248 1053 127
MspA1I CmG'CkG
Cuts at: 1871 2185 2545 3560 3805 1871
Size: 314 360 1015 245 2828
Fragments arranged by size:
2828 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2941 2941
Size: 4762
NarI GG'CG_CC
Cuts at: 165 2191 2439 165
Size: 2026 248 2488
Fragments arranged by size:
2488 2026 248
NcoI C'CATG_G
Cuts at: 125 134 1502 125
Size: 9 1368 3385
Fragments arranged by size:
3385 1368 9
NgoMIV G'CCGG_C
Cuts at: 2939 2939
Size: 4762
NruI TCG'CGA
Cuts at: 3123 3123
Size: 4762
NspI r_CATG'y
Cuts at: 884 1642 1813 2313 3222 884
Size: 758 171 500 909 2424
Fragments arranged by size:
2424 909 758 500 171
PciI A'CATG_T
Cuts at: 880 3218 880
Size: 2338 2424
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3901 3933 3901
Size: 32 4730
PpuMI rG'GwC_Cy
Cuts at: 140 1105 140
Size: 965 3797
PshAI GACnn'nnGTC
Cuts at: 934 1552 934
Size: 618 4144
PsiI TTA'TAA
Cuts at: 947 947
Size: 4762
PstI C_TGCA'G
Cuts at: 1807 2492 1807
Size: 685 4077
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4762
PvuII CAG'CTG
Cuts at: 2185 2545 2185
Size: 360 4402
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4762
SacI G_AGCT'C
Cuts at: 1169 1774 1169
Size: 605 4157
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4762
SanDI GG'GwC_CC
Cuts at: 140 140
Size: 4762
SapI GCTCTTCn'nnn_
Cuts at: 2783 2993 2783
Size: 210 4552
SbfI CC_TGCA'GG
Cuts at: 1807 1807
Size: 4762
ScaI AGT'ACT
Cuts at: 4279 4279
Size: 4762
SfcI C'TryA_G
Cuts at: 33 254 710 1803 2488 3483 3674 33
Size: 221 456 1093 685 995 191 1121
Fragments arranged by size:
1121 1093 995 685 456 221 191
SfoI GGC'GCC
Cuts at: 166 2192 2440 166
Size: 2026 248 2488
Fragments arranged by size:
2488 2026 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4762
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4762
SmlI C'TyrA_G
Cuts at: 217 226 357 1915 2830 3324 3586 217
Size: 9 131 1558 915 494 262 1393
Fragments arranged by size:
1558 1393 915 494 262 131 9
SnaBI TAC'GTA
Cuts at: 1752 1752
Size: 4762
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4762
SphI G_CATG'C
Cuts at: 1813 2313 1813
Size: 500 4262
StyI C'CwwG_G
Cuts at: 125 134 1502 1894 2248 125
Size: 9 1368 392 354 2639
Fragments arranged by size:
2639 1368 392 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 515 527 848 912 1564 2343 2476 2935 3146
Size: 12 321 64 652 779 133 459 211
Cuts at: 3146 515
Size: 2131
Fragments arranged by size:
2131 779 652 459 321 211 133 64
12
TatI w'GTAC_w
Cuts at: 1200 4277 1200
Size: 3077 1685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4279 4311 4279
Size: 32 4730
Tth111I GACn'n_nGTC
Cuts at: 2557 2557
Size: 4762
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 396 2255 132
Size: 264 1859 2639
Fragments arranged by size:
2639 1859 264
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4762
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI
NsiI PacI PasI PflMI PfoI PmlI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI