Restriction map for pF1KSDA0108
 (Circular) (Six-base) MAPSORT of: pF1KSDA0108.seq  Check: 9696  from: 1  to: 3836



pF1KSDA0108  3836 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    874     874 
   Size:       3836
 
Acc65I G'GTAC_C

Cuts at:    852     852 
   Size:       3836
 
AclI AA'CG_TT

Cuts at:   3676    3676 
   Size:       3836
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1661    2093    2840    3108    1661 
   Size:        432     747     268    2389

  Fragments arranged by size: 

                2389     747     432     268
 
AflIII A'CryG_T

Cuts at:   1188    2292    3269    1188 
   Size:       1104     977    1755

  Fragments arranged by size: 

                1755    1104     977
 
AgeI A'CCGG_T

Cuts at:    161    2224     161 
   Size:       2063    1773
 
AlwNI CAG_nnn'CTG

Cuts at:   1141    2708    1141 
   Size:       1567    2269
 
ApaLI G'TGCA_C

Cuts at:   2606    2606 
   Size:       3836
 
ApoI r'AATT_y

Cuts at:    840    3189    3445     840 
   Size:       2349     256    1231

  Fragments arranged by size: 

                2349    1231     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3836
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3836
 
AvaI C'yCGr_G

Cuts at:    856     856 
   Size:       3836
 
BamHI G'GATC_C

Cuts at:    861     861 
   Size:       3836
 
BanI G'GyrC_C

Cuts at:    852    1266    1514    1549     852 
   Size:        414     248      35    3139

  Fragments arranged by size: 

                3139     414     248      35
 
BanII G_rGCy'C

Cuts at:    850    1880     850 
   Size:       1030    2806
 
BbeI G_GCGC'C

Cuts at:   1270    1518    1270 
   Size:        248    3588
 
BciVI GTATCCnnnnn_n'

Cuts at:   1734    2495    3836    1734 
   Size:        761    1341    1734

  Fragments arranged by size: 

                1734    1341     761
 
BclI T'GATC_A

Cuts at:   1356    1356 
   Size:       3836
 
BglII A'GATC_T

Cuts at:   1351    3388    1351 
   Size:       2037    1799
 
BlpI GC'TnA_GC

Cuts at:    948     948 
   Size:       3836
 
Bme1580I G_kGCm'C

Cuts at:   1461    1554    2610    1461 
   Size:         93    1056    2687

  Fragments arranged by size: 

                2687    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1048    1129    1464    3608    1048 
   Size:         81     335    2144    1276

  Fragments arranged by size: 

                2144    1276     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2203    2203 
   Size:       3836
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    582     582 
   Size:       3836
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1012    1891    2383    2681    1012 
   Size:        879     492     298    2167

  Fragments arranged by size: 

                2167     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3540    3540 
   Size:       3836
 
BsaAI yAC'GTr

Cuts at:    828    1189     828 
   Size:        361    3475
 
BsaBI GATnn'nnATC

Cuts at:      6    1350    3387       6 
   Size:       1344    2037     455

  Fragments arranged by size: 

                2037    1344     455
 
BsaHI Gr'CG_yC

Cuts at:   1267    1515    2217    3713    1267 
   Size:        248     702    1496    1390

  Fragments arranged by size: 

                1496    1390     702     248
 
BsaWI w'CCGG_w

Cuts at:    161    1025    1546    2224    2498    2645    3056     161 
   Size:        864     521     678     274     147     411     941

  Fragments arranged by size: 

                 941     864     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    737     737 
   Size:       3836
 
BseYI C'CCAG_C

Cuts at:   1261    2019    2596    3092    1261 
   Size:        758     577     496    2005

  Fragments arranged by size: 

                2005     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1378    1424    2632     104 
   Size:       1274      46    1208    1308

  Fragments arranged by size: 

                1308    1274    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    850    1628    1818    2610     850 
   Size:        778     190     792    2076

  Fragments arranged by size: 

                2076     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    387     387 
   Size:       3836
 
Bsp1286I G_dGCh'C

Cuts at:    850    1461    1554    1628    1818    1880    2610     850 
   Size:        611      93      74     190      62     730    2076

  Fragments arranged by size: 

                2076     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3056    3056 
   Size:       3836
 
BspHI T'CATG_A

Cuts at:   3012    3012 
   Size:       3836
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    828     886    1100    1402    1783     828 
   Size:         58     214     302     381    2881

  Fragments arranged by size: 

                2881     381     302     214      58
 
BsrBI CCG'CTC

Cuts at:     50    2128    2182    3666    3831      50 
   Size:       2078      54    1484     165      55

  Fragments arranged by size: 

                2078    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1748    1748 
   Size:       3836
 
BsrFI r'CCGG_y

Cuts at:    161    1834    2015    2224     161 
   Size:       1673     181     209    1773

  Fragments arranged by size: 

                1773    1673     209     181
 
BssSI C'ACGA_G

Cuts at:   2107    2465    2107 
   Size:        358    3478
 
BstAPI GCAn_nnn'nTGC

Cuts at:    179    1213     179 
   Size:       1034    2802
 
BstBI TT'CG_AA

Cuts at:    110    2197     110 
   Size:       2087    1749
 
BstYI r'GATC_y

Cuts at:      1     861    1328    1351    1686    1932    2944    3030    3042 
   Size:        860     467      23     335     246    1012      86      12

Cuts at:   3042    3388       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     860     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       3836
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1786    1967    2130    1786 
   Size:        181     163    3492

  Fragments arranged by size: 

                3492     181     163
 
BtsI GCAGTG_nn'

Cuts at:    162    1079     162 
   Size:        917    2919
 
DraI TTT'AAA

Cuts at:    835     835 
   Size:       3836
 
DraIII CAC_nnn'GTG

Cuts at:    179     179 
   Size:       3836
 
DrdI GACnn_nn'nnGTC

Cuts at:   1542    2400    1542 
   Size:        858    2978
 
EaeI y'GGCC_r

Cuts at:    776    1421    1595    1986    2013    3358     776 
   Size:        645     174     391      27    1345    1254

  Fragments arranged by size: 

                1345    1254     645     391     174      27
 
EagI C'GGCC_G

Cuts at:   1421    1421 
   Size:       3836
 
EarI CTCTTCn'nnn_

Cuts at:   1859    2069    1859 
   Size:        210    3626
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2044    2354    2500    3646    2044 
   Size:        310     146    1146    2234

  Fragments arranged by size: 

                2234    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    848     848 
   Size:       3836
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    582    1661    2093    2840    3108     582 
   Size:       1079     432     747     268    1310

  Fragments arranged by size: 

                1310    1079     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    224     975     224 
   Size:        751    3085
 
EcoRI G'AATT_C

Cuts at:    840     840 
   Size:       3836
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1855    1855 
   Size:       3836
 
FspI TGC'GCA

Cuts at:   1617    1617 
   Size:       3836
 
HaeII r_GCGC'y

Cuts at:   1270    1518    2540    3519    1270 
   Size:        248    1022     979    1587

  Fragments arranged by size: 

                1587    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1678    1708    1710    1740    1678 
   Size:         30       2      30    3774

  Fragments arranged by size: 

                3774      30      30       2
 
HincII GTy'rAC

Cuts at:    306     875     306 
   Size:        569    3267
 
KasI G'GCGC_C

Cuts at:   1266    1514    1266 
   Size:        248    3588
 
KpnI G_GTAC'C

Cuts at:    856     856 
   Size:       3836
 
MluI A'CGCG_T

Cuts at:   3269    3269 
   Size:       3836
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2507    2691    2507 
   Size:        184    3652
 
MscI TGG'CCA

Cuts at:   1597    1597 
   Size:       3836
 
MslI CAynn'nnrTG

Cuts at:   1825    1952    1825 
   Size:        127    3709
 
MspA1I CmG'CkG

Cuts at:    301     602     947    1261    1621    2634    2879     301 
   Size:        301     345     314     360    1013     245    1258

  Fragments arranged by size: 

                1258    1013     360     345     314     301     245
 
NaeI GCC'GGC

Cuts at:   2017    2017 
   Size:       3836
 
NarI GG'CG_CC

Cuts at:   1267    1515    1267 
   Size:        248    3588
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       3836
 
NgoMIV G'CCGG_C

Cuts at:   2015    2015 
   Size:       3836
 
NspI r_CATG'y

Cuts at:    889    1389    2296     889 
   Size:        500     907    2429

  Fragments arranged by size: 

                2429     907     500
 
PciI A'CATG_T

Cuts at:   2292    2292 
   Size:       3836
 
PmeI GTTT'AAAC

Cuts at:    835     835 
   Size:       3836
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2975    3007    2975 
   Size:         32    3804
 
PpuMI rG'GwC_Cy

Cuts at:    224     224 
   Size:       3836
 
PsiI TTA'TAA

Cuts at:    400     400 
   Size:       3836
 
PstI C_TGCA'G

Cuts at:    883    1568     883 
   Size:        685    3151
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3836
 
PvuII CAG'CTG

Cuts at:    301     602    1261    1621     301 
   Size:        301     659     360    2516

  Fragments arranged by size: 

                2516     659     360     301
 
RsrII CG'GwC_CG

Cuts at:   2031    2031 
   Size:       3836
 
SacI G_AGCT'C

Cuts at:    850     850 
   Size:       3836
 
SalI G'TCGA_C

Cuts at:    873     873 
   Size:       3836
 
SapI GCTCTTCn'nnn_

Cuts at:   1859    2069    1859 
   Size:        210    3626
 
SbfI CC_TGCA'GG

Cuts at:    883     883 
   Size:       3836
 
ScaI AGT'ACT

Cuts at:   3353    3353 
   Size:       3836
 
SexAI A'CCwGG_T

Cuts at:    226     226 
   Size:       3836
 
SfcI C'TryA_G

Cuts at:     33     168     879    1564    2557    2748      33 
   Size:        135     711     685     993     191    1121

  Fragments arranged by size: 

                1121     993     711     685     191     135
 
SfoI GGC'GCC

Cuts at:   1268    1516    1268 
   Size:        248    3588
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3836
 
SmaI CCC'GGG

Cuts at:    858     858 
   Size:       3836
 
SmlI C'TyrA_G

Cuts at:    991    1906    2398    2660     991 
   Size:        915     492     262    2167

  Fragments arranged by size: 

                2167     915     492     262
 
SnaBI TAC'GTA

Cuts at:    828     828 
   Size:       3836
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3836
 
SphI G_CATG'C

Cuts at:    889    1389     889 
   Size:        500    3336
 
StyI C'CwwG_G

Cuts at:    125     590     970    1324     125 
   Size:        465     380     354    2637

  Fragments arranged by size: 

                2637     465     380     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1419    1552    2011    2220    1419 
   Size:        133     459     209    3035

  Fragments arranged by size: 

                3035     459     209     133
 
TatI w'GTAC_w

Cuts at:   3351    3351 
   Size:       3836
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    141     173    3353    3385     141 
   Size:         32    3180      32     592

  Fragments arranged by size: 

                3180     592      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    484    1633     484 
   Size:       1149    2687
 
XbaI T'CTAG_A

Cuts at:    867     867 
   Size:       3836
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1331    1331 
   Size:       3836
 
XmaI C'CCGG_G

Cuts at:    856     856 
   Size:       3836
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI
    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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