(Circular) (Six-base) MAPSORT of: pF1KSDA0108.seq Check: 9696 from: 1 to: 3836 pF1KSDA0108 3836 bp With 182 enzymes: SgfI * June 20, 2008 10:32 .. AccI GT'mk_AC Cuts at: 874 874 Size: 3836 Acc65I G'GTAC_C Cuts at: 852 852 Size: 3836 AclI AA'CG_TT Cuts at: 3676 3676 Size: 3836 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1661 2093 2840 3108 1661 Size: 432 747 268 2389 Fragments arranged by size: 2389 747 432 268 AflIII A'CryG_T Cuts at: 1188 2292 3269 1188 Size: 1104 977 1755 Fragments arranged by size: 1755 1104 977 AgeI A'CCGG_T Cuts at: 161 2224 161 Size: 2063 1773 AlwNI CAG_nnn'CTG Cuts at: 1141 2708 1141 Size: 1567 2269 ApaLI G'TGCA_C Cuts at: 2606 2606 Size: 3836 ApoI r'AATT_y Cuts at: 840 3189 3445 840 Size: 2349 256 1231 Fragments arranged by size: 2349 1231 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3836 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3836 AvaI C'yCGr_G Cuts at: 856 856 Size: 3836 BamHI G'GATC_C Cuts at: 861 861 Size: 3836 BanI G'GyrC_C Cuts at: 852 1266 1514 1549 852 Size: 414 248 35 3139 Fragments arranged by size: 3139 414 248 35 BanII G_rGCy'C Cuts at: 850 1880 850 Size: 1030 2806 BbeI G_GCGC'C Cuts at: 1270 1518 1270 Size: 248 3588 BciVI GTATCCnnnnn_n' Cuts at: 1734 2495 3836 1734 Size: 761 1341 1734 Fragments arranged by size: 1734 1341 761 BclI T'GATC_A Cuts at: 1356 1356 Size: 3836 BglII A'GATC_T Cuts at: 1351 3388 1351 Size: 2037 1799 BlpI GC'TnA_GC Cuts at: 948 948 Size: 3836 Bme1580I G_kGCm'C Cuts at: 1461 1554 2610 1461 Size: 93 1056 2687 Fragments arranged by size: 2687 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1048 1129 1464 3608 1048 Size: 81 335 2144 1276 Fragments arranged by size: 2144 1276 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 3836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 582 582 Size: 3836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1012 1891 2383 2681 1012 Size: 879 492 298 2167 Fragments arranged by size: 2167 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3540 3540 Size: 3836 BsaAI yAC'GTr Cuts at: 828 1189 828 Size: 361 3475 BsaBI GATnn'nnATC Cuts at: 6 1350 3387 6 Size: 1344 2037 455 Fragments arranged by size: 2037 1344 455 BsaHI Gr'CG_yC Cuts at: 1267 1515 2217 3713 1267 Size: 248 702 1496 1390 Fragments arranged by size: 1496 1390 702 248 BsaWI w'CCGG_w Cuts at: 161 1025 1546 2224 2498 2645 3056 161 Size: 864 521 678 274 147 411 941 Fragments arranged by size: 941 864 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 737 737 Size: 3836 BseYI C'CCAG_C Cuts at: 1261 2019 2596 3092 1261 Size: 758 577 496 2005 Fragments arranged by size: 2005 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1378 1424 2632 104 Size: 1274 46 1208 1308 Fragments arranged by size: 1308 1274 1208 46 BsiHKAI G_wGCw'C Cuts at: 850 1628 1818 2610 850 Size: 778 190 792 2076 Fragments arranged by size: 2076 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 387 387 Size: 3836 Bsp1286I G_dGCh'C Cuts at: 850 1461 1554 1628 1818 1880 2610 850 Size: 611 93 74 190 62 730 2076 Fragments arranged by size: 2076 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3056 3056 Size: 3836 BspHI T'CATG_A Cuts at: 3012 3012 Size: 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 828 886 1100 1402 1783 828 Size: 58 214 302 381 2881 Fragments arranged by size: 2881 381 302 214 58 BsrBI CCG'CTC Cuts at: 50 2128 2182 3666 3831 50 Size: 2078 54 1484 165 55 Fragments arranged by size: 2078 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1748 1748 Size: 3836 BsrFI r'CCGG_y Cuts at: 161 1834 2015 2224 161 Size: 1673 181 209 1773 Fragments arranged by size: 1773 1673 209 181 BssSI C'ACGA_G Cuts at: 2107 2465 2107 Size: 358 3478 BstAPI GCAn_nnn'nTGC Cuts at: 179 1213 179 Size: 1034 2802 BstBI TT'CG_AA Cuts at: 110 2197 110 Size: 2087 1749 BstYI r'GATC_y Cuts at: 1 861 1328 1351 1686 1932 2944 3030 3042 Size: 860 467 23 335 246 1012 86 12 Cuts at: 3042 3388 1 Size: 346 449 Fragments arranged by size: 1012 860 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 125 125 Size: 3836 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1786 1967 2130 1786 Size: 181 163 3492 Fragments arranged by size: 3492 181 163 BtsI GCAGTG_nn' Cuts at: 162 1079 162 Size: 917 2919 DraI TTT'AAA Cuts at: 835 835 Size: 3836 DraIII CAC_nnn'GTG Cuts at: 179 179 Size: 3836 DrdI GACnn_nn'nnGTC Cuts at: 1542 2400 1542 Size: 858 2978 EaeI y'GGCC_r Cuts at: 776 1421 1595 1986 2013 3358 776 Size: 645 174 391 27 1345 1254 Fragments arranged by size: 1345 1254 645 391 174 27 EagI C'GGCC_G Cuts at: 1421 1421 Size: 3836 EarI CTCTTCn'nnn_ Cuts at: 1859 2069 1859 Size: 210 3626 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2044 2354 2500 3646 2044 Size: 310 146 1146 2234 Fragments arranged by size: 2234 1146 310 146 EcoICRI GAG'CTC Cuts at: 848 848 Size: 3836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 582 1661 2093 2840 3108 582 Size: 1079 432 747 268 1310 Fragments arranged by size: 1310 1079 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 224 975 224 Size: 751 3085 EcoRI G'AATT_C Cuts at: 840 840 Size: 3836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1855 1855 Size: 3836 FspI TGC'GCA Cuts at: 1617 1617 Size: 3836 HaeII r_GCGC'y Cuts at: 1270 1518 2540 3519 1270 Size: 248 1022 979 1587 Fragments arranged by size: 1587 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1678 1708 1710 1740 1678 Size: 30 2 30 3774 Fragments arranged by size: 3774 30 30 2 HincII GTy'rAC Cuts at: 306 875 306 Size: 569 3267 KasI G'GCGC_C Cuts at: 1266 1514 1266 Size: 248 3588 KpnI G_GTAC'C Cuts at: 856 856 Size: 3836 MluI A'CGCG_T Cuts at: 3269 3269 Size: 3836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2507 2691 2507 Size: 184 3652 MscI TGG'CCA Cuts at: 1597 1597 Size: 3836 MslI CAynn'nnrTG Cuts at: 1825 1952 1825 Size: 127 3709 MspA1I CmG'CkG Cuts at: 301 602 947 1261 1621 2634 2879 301 Size: 301 345 314 360 1013 245 1258 Fragments arranged by size: 1258 1013 360 345 314 301 245 NaeI GCC'GGC Cuts at: 2017 2017 Size: 3836 NarI GG'CG_CC Cuts at: 1267 1515 1267 Size: 248 3588 NcoI C'CATG_G Cuts at: 125 125 Size: 3836 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 3836 NspI r_CATG'y Cuts at: 889 1389 2296 889 Size: 500 907 2429 Fragments arranged by size: 2429 907 500 PciI A'CATG_T Cuts at: 2292 2292 Size: 3836 PmeI GTTT'AAAC Cuts at: 835 835 Size: 3836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2975 3007 2975 Size: 32 3804 PpuMI rG'GwC_Cy Cuts at: 224 224 Size: 3836 PsiI TTA'TAA Cuts at: 400 400 Size: 3836 PstI C_TGCA'G Cuts at: 883 1568 883 Size: 685 3151 PvuI CG_AT'CG Cuts at: 104 104 Size: 3836 PvuII CAG'CTG Cuts at: 301 602 1261 1621 301 Size: 301 659 360 2516 Fragments arranged by size: 2516 659 360 301 RsrII CG'GwC_CG Cuts at: 2031 2031 Size: 3836 SacI G_AGCT'C Cuts at: 850 850 Size: 3836 SalI G'TCGA_C Cuts at: 873 873 Size: 3836 SapI GCTCTTCn'nnn_ Cuts at: 1859 2069 1859 Size: 210 3626 SbfI CC_TGCA'GG Cuts at: 883 883 Size: 3836 ScaI AGT'ACT Cuts at: 3353 3353 Size: 3836 SexAI A'CCwGG_T Cuts at: 226 226 Size: 3836 SfcI C'TryA_G Cuts at: 33 168 879 1564 2557 2748 33 Size: 135 711 685 993 191 1121 Fragments arranged by size: 1121 993 711 685 191 135 SfoI GGC'GCC Cuts at: 1268 1516 1268 Size: 248 3588 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3836 SmaI CCC'GGG Cuts at: 858 858 Size: 3836 SmlI C'TyrA_G Cuts at: 991 1906 2398 2660 991 Size: 915 492 262 2167 Fragments arranged by size: 2167 915 492 262 SnaBI TAC'GTA Cuts at: 828 828 Size: 3836 SpeI A'CTAG_T Cuts at: 67 67 Size: 3836 SphI G_CATG'C Cuts at: 889 1389 889 Size: 500 3336 StyI C'CwwG_G Cuts at: 125 590 970 1324 125 Size: 465 380 354 2637 Fragments arranged by size: 2637 465 380 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1419 1552 2011 2220 1419 Size: 133 459 209 3035 Fragments arranged by size: 3035 459 209 133 TatI w'GTAC_w Cuts at: 3351 3351 Size: 3836 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 141 173 3353 3385 141 Size: 32 3180 32 592 Fragments arranged by size: 3180 592 32 32 Tth111I GACn'n_nGTC Cuts at: 484 1633 484 Size: 1149 2687 XbaI T'CTAG_A Cuts at: 867 867 Size: 3836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1331 1331 Size: 3836 XmaI C'CCGG_G Cuts at: 856 856 Size: 3836 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI