Restriction map for
pF1KSDA0112
(Circular) (Six-base) MAPSORT of: pF1KSDA0112.seq Check: 5079 from: 1 to: 4232
pF1KSDA0112 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2489 3236 3504 2057
Size: 432 747 268 2785
Fragments arranged by size:
2785 747 432 268
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 217 217
Size: 4232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 619
Size: 32 4200
AlwNI CAG_nnn'CTG
Cuts at: 315 1537 3104 315
Size: 1222 1567 1443
Fragments arranged by size:
1567 1443 1222
ApaI G_GGCC'C
Cuts at: 1105 1105
Size: 4232
ApaLI G'TGCA_C
Cuts at: 202 3002 202
Size: 2800 1432
ApoI r'AATT_y
Cuts at: 1152 1236 3585 3841 1152
Size: 84 2349 256 1543
Fragments arranged by size:
2349 1543 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 572 860 1252 572
Size: 288 392 3552
Fragments arranged by size:
3552 392 288
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 603 855 1248 1662 1910 1945 603
Size: 252 393 414 248 35 2890
Fragments arranged by size:
2890 414 393 252 248 35
BanII G_rGCy'C
Cuts at: 410 868 1105 1246 2276 410
Size: 458 237 141 1030 2366
Fragments arranged by size:
2366 1030 458 237 141
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 514 514
Size: 4232
BbvCI CC'TCA_GC
Cuts at: 965 965
Size: 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 255 289 255
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 488 1052 488
Size: 564 3668
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 206 608 860 1105 1857 1950 3006 206
Size: 402 252 245 752 93 1056 1432
Fragments arranged by size:
1432 1056 752 402 252 245 93
BmrI ACTGGGnnnn_n'
Cuts at: 1444 1525 1860 4004 1444
Size: 81 335 2144 1672
Fragments arranged by size:
2144 1672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 4232
Bpu10I CC'TnA_GC
Cuts at: 965 965
Size: 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1408 2287 2779 3077 469
Size: 939 879 492 298 1624
Fragments arranged by size:
1624 939 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 810 3936 810
Size: 3126 1106
BsaAI yAC'GTr
Cuts at: 1224 1585 1224
Size: 361 3871
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 247 1663 1911 2613 4109 247
Size: 1416 248 702 1496 370
Fragments arranged by size:
1496 1416 702 370 248
BsaWI w'CCGG_w
Cuts at: 254 754 1421 1942 2620 2894 3041 3452 254
Size: 500 667 521 678 274 147 411 1034
Fragments arranged by size:
1034 678 667 521 500 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 208 238 619 649 208
Size: 30 381 30 3791
Fragments arranged by size:
3791 381 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 787 1021 1033 1047 1227 160
Size: 627 234 12 14 180 3165
Fragments arranged by size:
3165 627 234 180 14 12
BseYI C'CCAG_C
Cuts at: 554 730 777 1108 1657 2415 2992 3488 554
Size: 176 47 331 549 758 577 496 1298
Fragments arranged by size:
1298 758 577 549 496 331 176 47
BsiEI CG_ry'CG
Cuts at: 104 269 448 740 1774 1820 3028 104
Size: 165 179 292 1034 46 1208 1308
Fragments arranged by size:
1308 1208 1034 292 179 165 46
BsiHKAI G_wGCw'C
Cuts at: 206 1246 2024 2214 3006 206
Size: 1040 778 190 792 1432
Fragments arranged by size:
1432 1040 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 206 410 608 860 868 1105 1246 1857 1950
Size: 204 198 252 8 237 141 611 93
Cuts at: 1950 2024 2214 2276 3006 206
Size: 74 190 62 730 1432
Fragments arranged by size:
1432 730 611 252 237 204 198 190
141 93 74 62 8
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 947 3408 947
Size: 2461 1771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 1282 1496 1798 2179 248
Size: 1034 214 302 381 2301
Fragments arranged by size:
2301 1034 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 618 2144 618
Size: 1526 2706
BsrFI r'CCGG_y
Cuts at: 689 739 1070 2230 2411 2620 689
Size: 50 331 1160 181 209 2301
Fragments arranged by size:
2301 1160 331 209 181 50
BssHII G'CGCG_C
Cuts at: 478 567 710 478
Size: 89 143 4000
Fragments arranged by size:
4000 143 89
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 720 1609 720
Size: 889 3343
BstBI TT'CG_AA
Cuts at: 110 2593 110
Size: 2483 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 795 795
Size: 4232
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 365 448 125
Size: 240 83 3909
Fragments arranged by size:
3909 240 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 1475 1475
Size: 4232
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 451 537 671 737 797 1817 1991 2382 2409
Size: 86 134 66 60 1020 174 391 27
Cuts at: 2409 3754 451
Size: 1345 929
Fragments arranged by size:
1345 1020 929 391 174 134 86 66
60 27
EagI C'GGCC_G
Cuts at: 737 1817 737
Size: 1080 3152
EarI CTCTTCn'nnn_
Cuts at: 374 1117 2255 2465 374
Size: 743 1138 210 2141
Fragments arranged by size:
2141 1138 743 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 2057 2489 3236 3504 235
Size: 1822 432 747 268 963
Fragments arranged by size:
1822 963 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 844 844
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 312 1078 1101 1371 312
Size: 766 23 270 3173
Fragments arranged by size:
3173 766 270 23
EcoRI G'AATT_C
Cuts at: 1152 1236 1152
Size: 84 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 555 828 840 1200 1666 1914 2936 3915 555
Size: 273 12 360 466 248 1022 979 872
Fragments arranged by size:
1022 979 872 466 360 273 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2074 2104 2106 2136 2074
Size: 30 2 30 4170
Fragments arranged by size:
4170 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 911 2903 3087 230
Size: 681 1992 184 1375
Fragments arranged by size:
1992 1375 681 184
MscI TGG'CCA
Cuts at: 539 673 799 1993 539
Size: 134 126 1194 2778
Fragments arranged by size:
2778 1194 134 126
MslI CAynn'nnrTG
Cuts at: 199 793 2221 2348 199
Size: 594 1428 127 2083
Fragments arranged by size:
2083 1428 594 127
MspA1I CmG'CkG
Cuts at: 351 360 450 726 969 1343 1657 2017 3030
Size: 9 90 276 243 374 314 360 1013
Cuts at: 3030 3275 351
Size: 245 1308
Fragments arranged by size:
1308 1013 374 360 314 276 245 243
90 9
NaeI GCC'GGC
Cuts at: 741 1072 2413 741
Size: 331 1341 2560
Fragments arranged by size:
2560 1341 331
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 125 125
Size: 4232
NgoMIV G'CCGG_C
Cuts at: 739 1070 2411 739
Size: 331 1341 2560
Fragments arranged by size:
2560 1341 331
NspI r_CATG'y
Cuts at: 1285 1785 2692 1285
Size: 500 907 2825
Fragments arranged by size:
2825 907 500
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 141 590 141
Size: 449 3783
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 619 651 3371 3403 619
Size: 32 2720 32 1448
Fragments arranged by size:
2720 1448 32 32
PspOMI G'GGCC_C
Cuts at: 1101 1101
Size: 4232
PstI C_TGCA'G
Cuts at: 224 1279 1964 224
Size: 1055 685 2492
Fragments arranged by size:
2492 1055 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 351 360 726 969 1657 2017 351
Size: 9 366 243 688 360 2566
Fragments arranged by size:
2566 688 366 360 243 9
RsrII CG'GwC_CG
Cuts at: 252 2427 252
Size: 2175 2057
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SacII CC_GC'GG
Cuts at: 451 451
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SexAI A'CCwGG_T
Cuts at: 515 515
Size: 4232
SfcI C'TryA_G
Cuts at: 33 220 307 1275 1960 2953 3144 33
Size: 187 87 968 685 993 191 1121
Fragments arranged by size:
1121 993 968 685 191 187 87
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 574 862 1254 574
Size: 288 392 3552
Fragments arranged by size:
3552 392 288
SmlI C'TyrA_G
Cuts at: 484 1387 2302 2794 3056 484
Size: 903 915 492 262 1660
Fragments arranged by size:
1660 915 903 492 262
SnaBI TAC'GTA
Cuts at: 1224 1224
Size: 4232
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StyI C'CwwG_G
Cuts at: 125 623 800 845 1366 1720 125
Size: 498 177 45 521 354 2637
Fragments arranged by size:
2637 521 498 354 177 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1162 1815 1948 2407 2616 1162
Size: 653 133 459 209 2778
Fragments arranged by size:
2778 653 459 209 133
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 461 816 1727 372
Size: 89 355 911 2877
Fragments arranged by size:
2877 911 355 89
XmaI C'CCGG_G
Cuts at: 572 860 1252 572
Size: 288 392 3552
Fragments arranged by size:
3552 392 288
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI ZraI