Restriction map for
pF1KSDA0119
(Circular) (Six-base) MAPSORT of: pF1KSDA0119.seq Check: 8707 from: 1 to: 4108
pF1KSDA0119 4108 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4108
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4108
AclI AA'CG_TT
Cuts at: 3948 3948
Size: 4108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 2363 3112 3380 1931
Size: 432 749 268 2659
Fragments arranged by size:
2659 749 432 268
AflIII A'CryG_T
Cuts at: 1458 2564 3541 1458
Size: 1106 977 2025
Fragments arranged by size:
2025 1106 977
AgeI A'CCGG_T
Cuts at: 2496 2496
Size: 4108
AlwNI CAG_nnn'CTG
Cuts at: 1411 2980 1411
Size: 1569 2539
ApaLI G'TGCA_C
Cuts at: 2878 2878
Size: 4108
ApoI r'AATT_y
Cuts at: 1110 3461 3717 1110
Size: 2351 256 1501
Fragments arranged by size:
2351 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4108
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4108
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4108
BanI G'GyrC_C
Cuts at: 190 1122 1536 1784 1819 190
Size: 932 414 248 35 2479
Fragments arranged by size:
2479 932 414 248 35
BanII G_rGCy'C
Cuts at: 1120 2150 1120
Size: 1030 3078
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3860
BbsI GAAGACnn'nnnn_
Cuts at: 362 362
Size: 4108
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2767 4108 2004
Size: 763 1341 2004
Fragments arranged by size:
2004 1341 763
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4108
BglII A'GATC_T
Cuts at: 1621 3660 1621
Size: 2039 2069
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4108
Bme1580I G_kGCm'C
Cuts at: 1731 1824 2882 1731
Size: 93 1058 2957
Fragments arranged by size:
2957 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 835 1318 1399 1734 3880 835
Size: 483 81 335 2146 1063
Fragments arranged by size:
2146 1063 483 335 81
BmtI G_CTAG'C
Cuts at: 1064 1064
Size: 4108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 664 188
Size: 476 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1282 2161 2655 2953 655
Size: 627 879 494 298 1810
Fragments arranged by size:
1810 879 627 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3812 3812
Size: 4108
BsaAI yAC'GTr
Cuts at: 1098 1459 1098
Size: 361 3747
BsaBI GATnn'nnATC
Cuts at: 6 1620 3659 6
Size: 1614 2039 455
Fragments arranged by size:
2039 1614 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2489 3985 1537
Size: 248 704 1496 1660
Fragments arranged by size:
1660 1496 704 248
BsaWI w'CCGG_w
Cuts at: 719 1295 1816 2496 2770 2917 3328 719
Size: 576 521 680 274 147 411 1499
Fragments arranged by size:
1499 680 576 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 802
Size: 30 4078
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 4108
BseYI C'CCAG_C
Cuts at: 256 879 1531 2289 2868 3364 256
Size: 623 652 758 579 496 1000
Fragments arranged by size:
1000 758 652 623 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 988
Size: 4108
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2904 104
Size: 1544 46 1210 1308
Fragments arranged by size:
1544 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 780 830 1120 1898 2088 2882 780
Size: 50 290 778 190 794 2006
Fragments arranged by size:
2006 794 778 290 190 50
Bsp1286I G_dGCh'C
Cuts at: 780 830 1120 1731 1824 1898 2088 2150 2882
Size: 50 290 611 93 74 190 62 732
Cuts at: 2882 780
Size: 2006
Fragments arranged by size:
2006 732 611 290 190 93 74 62
50
BspEI T'CCGG_A
Cuts at: 3328 3328
Size: 4108
BspHI T'CATG_A
Cuts at: 3284 3284
Size: 4108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 294 960 1156 1370 1672 2053 294
Size: 666 196 214 302 381 2349
Fragments arranged by size:
2349 666 381 302 214 196
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3938 4103 50
Size: 2348 54 1486 165 55
Fragments arranged by size:
2348 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4108
BsrFI r'CCGG_y
Cuts at: 2104 2285 2496 2104
Size: 181 211 3716
Fragments arranged by size:
3716 211 181
BssSI C'ACGA_G
Cuts at: 2377 2737 2377
Size: 360 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4108
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4108
BstYI r'GATC_y
Cuts at: 1 552 784 1131 1598 1621 1956 2202 3216
Size: 551 232 347 467 23 335 246 1014
Cuts at: 3216 3302 3314 3660 1
Size: 86 12 346 449
Fragments arranged by size:
1014 551 467 449 347 346 335 246
232 86 23 12
BtgI C'CryG_G
Cuts at: 125 575 125
Size: 450 3658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3764
Fragments arranged by size:
3764 181 163
BtsI GCAGTG_nn'
Cuts at: 148 348 1349 148
Size: 200 1001 2907
Fragments arranged by size:
2907 1001 200
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4108
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2672 1812
Size: 860 3248
EaeI y'GGCC_r
Cuts at: 162 314 602 929 1691 1865 2256 2283 3630
Size: 152 288 327 762 174 391 27 1347
Cuts at: 3630 162
Size: 640
Fragments arranged by size:
1347 762 640 391 327 288 174 152
27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4108
EarI CTCTTCn'nnn_
Cuts at: 343 2129 2339 343
Size: 1786 210 2112
Fragments arranged by size:
2112 1786 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 253 2314 2626 2772 3918 253
Size: 2061 312 146 1146 443
Fragments arranged by size:
2061 1146 443 312 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 664 1931 2363 3112 3380 188
Size: 476 1267 432 749 268 916
Fragments arranged by size:
1267 916 749 476 432 268
EcoO109I rG'GnC_Cy
Cuts at: 837 1245 837
Size: 408 3700
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 242 2125 242
Size: 1883 2225
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4108
HaeII r_GCGC'y
Cuts at: 1540 1788 2812 3791 1540
Size: 248 1024 979 1857
Fragments arranged by size:
1857 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 302 334 802 834 1948 1978 1980 2010 302
Size: 32 468 32 1114 30 2 30 2400
Fragments arranged by size:
2400 1114 468 32 32 30 30 2
HincII GTy'rAC
Cuts at: 459 989 1145 459
Size: 530 156 3422
Fragments arranged by size:
3422 530 156
HindIII A'AGCT_T
Cuts at: 224 376 224
Size: 152 3956
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3860
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4108
MfeI C'AATT_G
Cuts at: 445 445
Size: 4108
MluI A'CGCG_T
Cuts at: 3541 3541
Size: 4108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 435 2779 2963 288
Size: 147 2344 184 1433
Fragments arranged by size:
2344 1433 184 147
MscI TGG'CCA
Cuts at: 164 316 604 931 1867 164
Size: 152 288 327 936 2405
Fragments arranged by size:
2405 936 327 288 152
MslI CAynn'nnrTG
Cuts at: 616 2095 2222 616
Size: 1479 127 2502
Fragments arranged by size:
2502 1479 127
MspA1I CmG'CkG
Cuts at: 642 906 1217 1531 1891 2906 3151 642
Size: 264 311 314 360 1015 245 1599
Fragments arranged by size:
1599 1015 360 314 311 264 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4108
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3860
NcoI C'CATG_G
Cuts at: 125 575 125
Size: 450 3658
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4108
NheI G'CTAG_C
Cuts at: 1060 1060
Size: 4108
NruI TCG'CGA
Cuts at: 2469 2469
Size: 4108
NspI r_CATG'y
Cuts at: 1159 1659 2568 1159
Size: 500 909 2699
Fragments arranged by size:
2699 909 500
PciI A'CATG_T
Cuts at: 2564 2564
Size: 4108
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3279 3247
Size: 32 4076
PpuMI rG'GwC_Cy
Cuts at: 837 837
Size: 4108
PstI C_TGCA'G
Cuts at: 911 996 1153 1838 911
Size: 85 157 685 3181
Fragments arranged by size:
3181 685 157 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4108
PvuII CAG'CTG
Cuts at: 642 906 1531 1891 642
Size: 264 625 360 2859
Fragments arranged by size:
2859 625 360 264
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4108
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4108
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4108
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3898
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4108
ScaI AGT'ACT
Cuts at: 3625 3625
Size: 4108
SexAI A'CCwGG_T
Cuts at: 692 692
Size: 4108
SfcI C'TryA_G
Cuts at: 33 771 907 992 1149 1834 2829 3020 33
Size: 738 136 85 157 685 995 191 1121
Fragments arranged by size:
1121 995 738 685 191 157 136 85
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3860
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4108
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4108
SmlI C'TyrA_G
Cuts at: 670 1261 2176 2670 2932 670
Size: 591 915 494 262 1846
Fragments arranged by size:
1846 915 591 494 262
SnaBI TAC'GTA
Cuts at: 1098 1098
Size: 4108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4108
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3608
SspI AAT'ATT
Cuts at: 539 1093 539
Size: 554 3554
StyI C'CwwG_G
Cuts at: 125 419 575 854 1240 1594 125
Size: 294 156 279 386 354 2639
Fragments arranged by size:
2639 386 354 294 279 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 795 1689 1822 2281 2492 795
Size: 894 133 459 211 2411
Fragments arranged by size:
2411 894 459 211 133
TatI w'GTAC_w
Cuts at: 674 3623 674
Size: 2949 1159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3625 3657 3625
Size: 32 4076
Tth111I GACn'n_nGTC
Cuts at: 841 1903 841
Size: 1062 3046
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 910 1601 910
Size: 691 3417
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4108
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NdeI NotI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI StuI SwaI XhoI
XmnI ZraI