Restriction map for
pF1KSDA0120
(Circular) (Six-base) MAPSORT of: pF1KSDA0120.seq Check: 9816 from: 1 to: 3736
pF1KSDA0120 3736 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3736
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3736
AclI AA'CG_TT
Cuts at: 3576 3576
Size: 3736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1991 2740 3008 1559
Size: 432 749 268 2287
Fragments arranged by size:
2287 749 432 268
AfeI AGC'GCT
Cuts at: 430 430
Size: 3736
AflIII A'CryG_T
Cuts at: 1086 2192 3169 1086
Size: 1106 977 1653
Fragments arranged by size:
1653 1106 977
AgeI A'CCGG_T
Cuts at: 2124 2124
Size: 3736
AlwNI CAG_nnn'CTG
Cuts at: 213 414 1039 2608 213
Size: 201 625 1569 1341
Fragments arranged by size:
1569 1341 625 201
ApaLI G'TGCA_C
Cuts at: 2506 2506
Size: 3736
ApoI r'AATT_y
Cuts at: 738 3089 3345 738
Size: 2351 256 1129
Fragments arranged by size:
2351 1129 256
AseI AT'TA_AT
Cuts at: 20 323 20
Size: 303 3433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3736
AvaI C'yCGr_G
Cuts at: 337 539 754 337
Size: 202 215 3319
Fragments arranged by size:
3319 215 202
BamHI G'GATC_C
Cuts at: 564 759 564
Size: 195 3541
BanI G'GyrC_C
Cuts at: 661 750 1164 1412 1447 661
Size: 89 414 248 35 2950
Fragments arranged by size:
2950 414 248 89 35
BanII G_rGCy'C
Cuts at: 320 554 748 1778 320
Size: 234 194 1030 2278
Fragments arranged by size:
2278 1030 234 194
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3488
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2395 3736 1632
Size: 763 1341 1632
Fragments arranged by size:
1632 1341 763
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3736
BglII A'GATC_T
Cuts at: 198 401 1249 3288 198
Size: 203 848 2039 646
Fragments arranged by size:
2039 848 646 203
BlpI GC'TnA_GC
Cuts at: 425 846 425
Size: 421 3315
Bme1580I G_kGCm'C
Cuts at: 664 1359 1452 2510 664
Size: 695 93 1058 1890
Fragments arranged by size:
1890 1058 695 93
BmrI ACTGGGnnnn_n'
Cuts at: 228 346 946 1027 1362 3508 228
Size: 118 600 81 335 2146 456
Fragments arranged by size:
2146 600 456 335 118 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 420 2101 420
Size: 1681 2055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 223
Size: 3736
Bpu10I CC'TnA_GC
Cuts at: 154 154
Size: 3736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 910 1789 2283 2581 277
Size: 633 879 494 298 1432
Fragments arranged by size:
1432 879 633 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3440 3440
Size: 3736
BsaAI yAC'GTr
Cuts at: 726 1087 726
Size: 361 3375
BsaBI GATnn'nnATC
Cuts at: 6 197 214 1248 3287 6
Size: 191 17 1034 2039 455
Fragments arranged by size:
2039 1034 455 191 17
BsaHI Gr'CG_yC
Cuts at: 676 1165 1413 2117 3613 676
Size: 489 248 704 1496 799
Fragments arranged by size:
1496 799 704 489 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2124 2398 2545 2956 923
Size: 521 680 274 147 411 1703
Fragments arranged by size:
1703 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 260 1159 1917 2496 2992 260
Size: 899 758 579 496 1004
Fragments arranged by size:
1004 899 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 520 187
Size: 333 3403
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2532 104
Size: 1172 46 1210 1308
Fragments arranged by size:
1308 1210 1172 46
BsiHKAI G_wGCw'C
Cuts at: 320 748 1526 1716 2510 320
Size: 428 778 190 794 1546
Fragments arranged by size:
1546 794 778 428 190
BsmBI CGTCTCn'nnnn_
Cuts at: 683 683
Size: 3736
Bsp1286I G_dGCh'C
Cuts at: 320 554 664 748 1359 1452 1526 1716 1778
Size: 234 110 84 611 93 74 190 62
Cuts at: 1778 2510 320
Size: 732 1546
Fragments arranged by size:
1546 732 611 234 190 110 93 84
74 62
BspEI T'CCGG_A
Cuts at: 2956 2956
Size: 3736
BspHI T'CATG_A
Cuts at: 2912 2912
Size: 3736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 150 784 998 1300 1681 150
Size: 634 214 302 381 2205
Fragments arranged by size:
2205 634 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3566 3731 50
Size: 1976 54 1486 165 55
Fragments arranged by size:
1976 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3736
BsrFI r'CCGG_y
Cuts at: 254 1732 1913 2124 254
Size: 1478 181 211 1866
Fragments arranged by size:
1866 1478 211 181
BssSI C'ACGA_G
Cuts at: 2005 2365 2005
Size: 360 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3736
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3736
BstYI r'GATC_y
Cuts at: 1 198 209 362 401 564 716 759 1226
Size: 197 11 153 39 163 152 43 467
Cuts at: 1226 1249 1584 1830 2844 2930 2942 3288 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 467 449 346 335 246 197 163
153 152 86 43 39 23 12 11
BtgI C'CryG_G
Cuts at: 125 377 669 125
Size: 252 292 3192
Fragments arranged by size:
3192 292 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3392
Fragments arranged by size:
3392 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3736
DraI TTT'AAA
Cuts at: 733 733
Size: 3736
DraIII CAC_nnn'GTG
Cuts at: 236 236
Size: 3736
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2300 1440
Size: 860 2876
EaeI y'GGCC_r
Cuts at: 128 1319 1493 1884 1911 3258 128
Size: 1191 174 391 27 1347 606
Fragments arranged by size:
1347 1191 606 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3736
EarI CTCTTCn'nnn_
Cuts at: 559 1757 1967 559
Size: 1198 210 2328
Fragments arranged by size:
2328 1198 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2254 2400 3546 1942
Size: 312 146 1146 2132
Fragments arranged by size:
2132 1146 312 146
EcoICRI GAG'CTC
Cuts at: 318 746 318
Size: 428 3308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1559 1991 2740 3008 223
Size: 1336 432 749 268 951
Fragments arranged by size:
1336 951 749 432 268
EcoO109I rG'GnC_Cy
Cuts at: 139 348 873 139
Size: 209 525 3002
Fragments arranged by size:
3002 525 209
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3736
FseI GG_CCGG'CC
Cuts at: 258 258
Size: 3736
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3736
HaeII r_GCGC'y
Cuts at: 432 1168 1416 2440 3419 432
Size: 736 248 1024 979 749
Fragments arranged by size:
1024 979 749 736 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3674
Fragments arranged by size:
3674 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3736
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3488
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3736
MluI A'CGCG_T
Cuts at: 3169 3169
Size: 3736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2407 2591 2407
Size: 184 3552
MscI TGG'CCA
Cuts at: 130 1495 130
Size: 1365 2371
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3609
MspA1I CmG'CkG
Cuts at: 424 504 624 671 845 1159 1519 2534 2779
Size: 80 120 47 174 314 360 1015 245
Cuts at: 2779 424
Size: 1381
Fragments arranged by size:
1381 1015 360 314 245 174 120 80
47
NaeI GCC'GGC
Cuts at: 256 1915 256
Size: 1659 2077
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3488
NcoI C'CATG_G
Cuts at: 125 377 125
Size: 252 3484
NdeI CA'TA_TG
Cuts at: 147 147
Size: 3736
NgoMIV G'CCGG_C
Cuts at: 254 1913 254
Size: 1659 2077
NruI TCG'CGA
Cuts at: 2097 2097
Size: 3736
NspI r_CATG'y
Cuts at: 787 1287 2196 787
Size: 500 909 2327
Fragments arranged by size:
2327 909 500
PciI A'CATG_T
Cuts at: 2192 2192
Size: 3736
PflMI CCAn_nnn'nTGG
Cuts at: 465 465
Size: 3736
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2907 2875
Size: 32 3704
PpuMI rG'GwC_Cy
Cuts at: 139 139
Size: 3736
PstI C_TGCA'G
Cuts at: 781 1466 781
Size: 685 3051
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3736
PvuII CAG'CTG
Cuts at: 424 624 1159 1519 424
Size: 200 535 360 2641
Fragments arranged by size:
2641 535 360 200
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3736
SacI G_AGCT'C
Cuts at: 320 748 320
Size: 428 3308
SacII CC_GC'GG
Cuts at: 672 672
Size: 3736
SalI G'TCGA_C
Cuts at: 771 771
Size: 3736
SapI GCTCTTCn'nnn_
Cuts at: 559 1757 1967 559
Size: 1198 210 2328
Fragments arranged by size:
2328 1198 210
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3736
ScaI AGT'ACT
Cuts at: 3253 3253
Size: 3736
SfcI C'TryA_G
Cuts at: 33 777 1462 2457 2648 33
Size: 744 685 995 191 1121
Fragments arranged by size:
1121 995 744 685 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3488
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3736
SmaI CCC'GGG
Cuts at: 756 756
Size: 3736
SmlI C'TyrA_G
Cuts at: 292 889 1804 2298 2560 292
Size: 597 915 494 262 1468
Fragments arranged by size:
1468 915 597 494 262
SnaBI TAC'GTA
Cuts at: 726 726
Size: 3736
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3736
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3236
StuI AGG'CCT
Cuts at: 370 370
Size: 3736
StyI C'CwwG_G
Cuts at: 125 377 590 868 1222 125
Size: 252 213 278 354 2639
Fragments arranged by size:
2639 354 278 252 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 247 511 1317 1450 1909 2120 247
Size: 264 806 133 459 211 1863
Fragments arranged by size:
1863 806 459 264 211 133
TatI w'GTAC_w
Cuts at: 3251 3251
Size: 3736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 701 733 3253 3285 701
Size: 32 2520 32 1152
Fragments arranged by size:
2520 1152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3736
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3736
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3736
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FspAI HindIII HpaI MfeI NheI NotI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI