Restriction map for
pF1KSDA0122
(Circular) (Six-base) MAPSORT of: pF1KSDA0122.seq Check: 2651 from: 1 to: 5924
pF1KSDA0122 5924 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 1829 1829
Size: 5924
AccI GT'mk_AC
Cuts at: 1421 1702 2660 2962 1421
Size: 281 958 302 4383
Fragments arranged by size:
4383 958 302 281
Acc65I G'GTAC_C
Cuts at: 1390 2940 1390
Size: 1550 4374
AclI AA'CG_TT
Cuts at: 5764 5764
Size: 5924
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 3749 4181 4928 5196 608
Size: 3141 432 747 268 1336
Fragments arranged by size:
3141 1336 747 432 268
AflIII A'CryG_T
Cuts at: 3276 4380 5357 3276
Size: 1104 977 3843
Fragments arranged by size:
3843 1104 977
AgeI A'CCGG_T
Cuts at: 4312 4312
Size: 5924
AhdI GACnn_n'nnGTC
Cuts at: 1593 1593
Size: 5924
AleI CACnn'nnGTG
Cuts at: 743 743
Size: 5924
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2731 2731
Size: 5924
AlwNI CAG_nnn'CTG
Cuts at: 547 864 897 930 1200 1209 1218 1938 2188
Size: 317 33 33 270 9 9 720 250
Cuts at: 2188 2197 2397 2656 3229 4796 547
Size: 9 200 259 573 1567 1675
Fragments arranged by size:
1675 1567 720 573 317 270 259 250
200 33 33 9 9 9
ApaI G_GGCC'C
Cuts at: 1083 1519 2100 2362 1083
Size: 436 581 262 4645
Fragments arranged by size:
4645 581 436 262
ApaLI G'TGCA_C
Cuts at: 2822 4694 2822
Size: 1872 4052
ApoI r'AATT_y
Cuts at: 2928 5277 5533 2928
Size: 2349 256 3319
Fragments arranged by size:
3319 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5924
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
AvaI C'yCGr_G
Cuts at: 345 416 1561 2212 2605 2678 2944 345
Size: 71 1145 651 393 73 266 3325
Fragments arranged by size:
3325 1145 651 393 266 73 71
BamHI G'GATC_C
Cuts at: 1542 2949 1542
Size: 1407 4517
BanI G'GyrC_C
Cuts at: 1086 1390 1507 2013 2450 2940 3354 3602 3637
Size: 304 117 506 437 490 414 248 35
Cuts at: 3637 1086
Size: 3373
Fragments arranged by size:
3373 506 490 437 414 304 248 117
35
BanII G_rGCy'C
Cuts at: 352 997 1083 1519 1739 1747 2100 2362 2471
Size: 645 86 436 220 8 353 262 109
Cuts at: 2471 2505 2621 2812 2938 3968 352
Size: 34 116 191 126 1030 2308
Fragments arranged by size:
2308 1030 645 436 353 262 220 191
126 116 109 86 34 8
BbeI G_GCGC'C
Cuts at: 3358 3606 3358
Size: 248 5676
BbsI GAAGACnn'nnnn_
Cuts at: 1469 1469
Size: 5924
BbvCI CC'TCA_GC
Cuts at: 523 523
Size: 5924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 803 837 2659 2693 803
Size: 34 1822 34 4034
Fragments arranged by size:
4034 1822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3822 4583 5924 3822
Size: 761 1341 3822
Fragments arranged by size:
3822 1341 761
BclI T'GATC_A
Cuts at: 3444 3444
Size: 5924
BglI GCCn_nnn'nGGC
Cuts at: 281 398 407 1327 1357 281
Size: 117 9 920 30 4848
Fragments arranged by size:
4848 920 117 30 9
BglII A'GATC_T
Cuts at: 3439 5476 3439
Size: 2037 3887
BlpI GC'TnA_GC
Cuts at: 919 3036 919
Size: 2117 3807
Bme1580I G_kGCm'C
Cuts at: 842 1083 1471 1510 1519 1681 2016 2100 2362
Size: 241 388 39 9 162 335 84 262
Cuts at: 2362 2826 3549 3642 4698 842
Size: 464 723 93 1056 2068
Fragments arranged by size:
2068 1056 723 464 388 335 262 241
162 93 84 39 9
BmgBI CAC'GTC
Cuts at: 142 142
Size: 5924
BmrI ACTGGGnnnn_n'
Cuts at: 913 1347 1899 1953 2904 3136 3217 3552 5696
Size: 434 552 54 951 232 81 335 2144
Cuts at: 5696 913
Size: 1141
Fragments arranged by size:
2144 1141 951 552 434 335 232 81
54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1023 4291 1023
Size: 3268 2656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 1723 1999 1571
Size: 152 276 5496
Fragments arranged by size:
5496 276 152
Bpu10I CC'TnA_GC
Cuts at: 523 2363 523
Size: 1840 4084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2506 3100 3979 4471 4769 1486
Size: 1020 594 879 492 298 2641
Fragments arranged by size:
2641 1020 879 594 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 215 1843 5628 215
Size: 1628 3785 511
Fragments arranged by size:
3785 1628 511
BsaAI yAC'GTr
Cuts at: 2916 3277 2916
Size: 361 5563
BsaBI GATnn'nnATC
Cuts at: 6 3438 5475 6
Size: 3432 2037 455
Fragments arranged by size:
3432 2037 455
BsaHI Gr'CG_yC
Cuts at: 794 1826 2418 3355 3603 4305 5801 794
Size: 1032 592 937 248 702 1496 917
Fragments arranged by size:
1496 1032 937 917 702 592 248
BsaWI w'CCGG_w
Cuts at: 3113 3634 4312 4586 4733 5144 3113
Size: 521 678 274 147 411 3893
Fragments arranged by size:
3893 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 325 355 1276 1306 1885 1915 325
Size: 30 921 30 579 30 4334
Fragments arranged by size:
4334 921 579 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 478 481 484 487 490 493 502 505
Size: 153 3 3 3 3 3 9 3
Cuts at: 505 508 511 1098 1423 2353 2356 2386 2640
Size: 3 3 587 325 930 3 30 254
Cuts at: 2640 325
Size: 3609
Fragments arranged by size:
3609 930 587 325 254 153 30 9
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 1193 1619 2093 2428 2688 3349 4107 4684 5180
Size: 426 474 335 260 661 758 577 496
Cuts at: 5180 1193
Size: 1937
Fragments arranged by size:
1937 758 661 577 496 474 426 335
260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2594 2860 1204
Size: 1390 266 4268
Fragments arranged by size:
4268 1390 266
BsiEI CG_ry'CG
Cuts at: 104 1421 1991 2283 3466 3512 4720 104
Size: 1317 570 292 1183 46 1208 1308
Fragments arranged by size:
1317 1308 1208 1183 570 292 46
BsiHKAI G_wGCw'C
Cuts at: 1727 1739 2229 2826 2938 3716 3906 4698 1727
Size: 12 490 597 112 778 190 792 2953
Fragments arranged by size:
2953 792 778 597 490 190 112 12
BsmBI CGTCTCn'nnnn_
Cuts at: 413 1833 413
Size: 1420 4504
Bsp1286I G_dGCh'C
Cuts at: 352 842 997 1083 1471 1510 1519 1681 1727
Size: 490 155 86 388 39 9 162 46
Cuts at: 1727 1739 1747 2016 2100 2229 2362 2471 2505
Size: 12 8 269 84 129 133 109 34
Cuts at: 2505 2621 2812 2826 2938 3549 3642 3716 3906
Size: 116 191 14 112 611 93 74 190
Cuts at: 3906 3968 4698 352
Size: 62 730 1578
Fragments arranged by size:
1578 730 611 490 388 269 191 190
162 155 133 129 116 112 109 93
86 84 74 62 46 39 34 14
12 9 8
BspEI T'CCGG_A
Cuts at: 5144 5144
Size: 5924
BspHI T'CATG_A
Cuts at: 5100 5100
Size: 5924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 2974 3188 3490 3871 1546
Size: 1428 214 302 381 3599
Fragments arranged by size:
3599 1428 381 302 214
BsrBI CCG'CTC
Cuts at: 50 188 2367 4216 4270 5754 5919 50
Size: 138 2179 1849 54 1484 165 55
Fragments arranged by size:
2179 1849 1484 165 138 55 54
BsrDI GCAATG_nn'
Cuts at: 1340 3836 1340
Size: 2496 3428
BsrFI r'CCGG_y
Cuts at: 397 1416 1773 1879 2495 3922 4103 4312 397
Size: 1019 357 106 616 1427 181 209 2009
Fragments arranged by size:
2009 1427 1019 616 357 209 181 106
BssSI C'ACGA_G
Cuts at: 876 4195 4553 876
Size: 3319 358 2247
Fragments arranged by size:
3319 2247 358
BstAPI GCAn_nnn'nTGC
Cuts at: 585 864 897 1209 1218 3301 585
Size: 279 33 312 9 2083 3208
Fragments arranged by size:
3208 2083 312 279 33 9
BstBI TT'CG_AA
Cuts at: 110 4285 110
Size: 4175 1749
BstEII G'GTnAC_C
Cuts at: 2844 2889 2844
Size: 45 5879
BstXI CCAn_nnnn'nTGG
Cuts at: 2085 2085
Size: 5924
BstYI r'GATC_y
Cuts at: 1 1205 1542 2256 2949 3416 3439 3774 4020
Size: 1204 337 714 693 467 23 335 246
Cuts at: 4020 5032 5118 5130 5476 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1204 1012 714 693 467 449 346 337
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1703 1703
Size: 5924
BtgI C'CryG_G
Cuts at: 125 561 1061 1152 1491 2726 125
Size: 436 500 91 339 1235 3323
Fragments arranged by size:
3323 1235 500 436 339 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3874 4055 4218 3874
Size: 181 163 5580
Fragments arranged by size:
5580 181 163
BtsI GCAGTG_nn'
Cuts at: 895 1334 1679 1787 2186 2700 2872 3167 895
Size: 439 345 108 399 514 172 295 3652
Fragments arranged by size:
3652 514 439 399 345 295 172 108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 771 806 771
Size: 35 5889
DraI TTT'AAA
Cuts at: 2923 2923
Size: 5924
DraIII CAC_nnn'GTG
Cuts at: 585 907 585
Size: 322 5602
DrdI GACnn_nn'nnGTC
Cuts at: 146 3630 4488 146
Size: 3484 858 1582
Fragments arranged by size:
3484 1582 858
EaeI y'GGCC_r
Cuts at: 165 564 909 1328 1988 2270 3509 3683 4074
Size: 399 345 419 660 282 1239 174 391
Cuts at: 4074 4101 5446 165
Size: 27 1345 643
Fragments arranged by size:
1345 1239 660 643 419 399 391 345
282 174 27
EagI C'GGCC_G
Cuts at: 1988 3509 1988
Size: 1521 4403
EarI CTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1974 4132 4442 4588 5734 1974
Size: 2158 310 146 1146 2164
Fragments arranged by size:
2164 2158 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1737 2936 1737
Size: 1199 4725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1571 1723 1999 3749 4181 4928 5196 608
Size: 963 152 276 1750 432 747 268 1336
Fragments arranged by size:
1750 1336 963 747 432 276 268 152
EcoNI CCTnn'n_nnAGG
Cuts at: 988 2297 988
Size: 1309 4615
EcoO109I rG'GnC_Cy
Cuts at: 927 1079 1080 1215 1515 1516 1558 2358 2359
Size: 152 1 135 300 1 42 800 1
Cuts at: 2359 3063 927
Size: 704 3788
Fragments arranged by size:
3788 800 704 300 152 135 42 1
1 1
EcoRI G'AATT_C
Cuts at: 2928 2928
Size: 5924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3943 3943
Size: 5924
FspI TGC'GCA
Cuts at: 1039 3705 1039
Size: 2666 3258
HaeII r_GCGC'y
Cuts at: 2443 3358 3606 4628 5607 2443
Size: 915 248 1022 979 2760
Fragments arranged by size:
2760 1022 979 915 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 870 902 1276 1308 1579 1611 1837
Size: 32 549 32 374 32 271 32 226
Cuts at: 1837 1869 1885 1917 3766 3796 3798 3828 289
Size: 32 16 32 1849 30 2 30 2385
Fragments arranged by size:
2385 1849 549 374 271 226 32 32
32 32 32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 1422 2963 1422
Size: 1541 4383
KasI G'GCGC_C
Cuts at: 3354 3602 3354
Size: 248 5676
KpnI G_GTAC'C
Cuts at: 1394 2944 1394
Size: 1550 4374
MluI A'CGCG_T
Cuts at: 5357 5357
Size: 5924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1136 2692 4595 4779 1136
Size: 1556 1903 184 2281
Fragments arranged by size:
2281 1903 1556 184
MscI TGG'CCA
Cuts at: 566 1330 2272 3685 566
Size: 764 942 1413 2805
Fragments arranged by size:
2805 1413 942 764
MslI CAynn'nnrTG
Cuts at: 293 517 743 2251 3913 4040 293
Size: 224 226 1508 1662 127 2177
Fragments arranged by size:
2177 1662 1508 226 224 127
MspA1I CmG'CkG
Cuts at: 570 1154 1197 1720 2321 2578 2747 3035 3349
Size: 584 43 523 601 257 169 288 314
Cuts at: 3349 3709 4722 4967 570
Size: 360 1013 245 1527
Fragments arranged by size:
1527 1013 601 584 523 360 314 288
257 245 169 43
NaeI GCC'GGC
Cuts at: 4105 4105
Size: 5924
NarI GG'CG_CC
Cuts at: 3355 3603 3355
Size: 248 5676
NcoI C'CATG_G
Cuts at: 125 1061 1491 2726 125
Size: 936 430 1235 3323
Fragments arranged by size:
3323 1235 936 430
NgoMIV G'CCGG_C
Cuts at: 4103 4103
Size: 5924
NruI TCG'CGA
Cuts at: 263 263
Size: 5924
NspI r_CATG'y
Cuts at: 2977 3477 4384 2977
Size: 500 907 4517
Fragments arranged by size:
4517 907 500
PasI CC'CwG_GG
Cuts at: 960 960
Size: 5924
PciI A'CATG_T
Cuts at: 4380 4380
Size: 5924
PflMI CCAn_nnn'nTGG
Cuts at: 907 2732 907
Size: 1825 4099
PmeI GTTT'AAAC
Cuts at: 2923 2923
Size: 5924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 5063
Size: 32 5892
PpuMI rG'GwC_Cy
Cuts at: 1558 1558
Size: 5924
PspOMI G'GGCC_C
Cuts at: 1079 1515 2096 2358 1079
Size: 436 581 262 4645
Fragments arranged by size:
4645 581 436 262
PstI C_TGCA'G
Cuts at: 575 1205 1214 2193 2723 2971 3656 575
Size: 630 9 979 530 248 685 2843
Fragments arranged by size:
2843 979 685 630 530 248 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5924
PvuII CAG'CTG
Cuts at: 570 1197 2578 3349 3709 570
Size: 627 1381 771 360 2785
Fragments arranged by size:
2785 1381 771 627 360
RsrII CG'GwC_CG
Cuts at: 449 4119 449
Size: 3670 2254
SacI G_AGCT'C
Cuts at: 1739 2938 1739
Size: 1199 4725
SacII CC_GC'GG
Cuts at: 1155 1155
Size: 5924
SalI G'TCGA_C
Cuts at: 1420 2961 1420
Size: 1541 4383
SapI GCTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
SbfI CC_TGCA'GG
Cuts at: 1214 2971 1214
Size: 1757 4167
ScaI AGT'ACT
Cuts at: 5441 5441
Size: 5924
SfcI C'TryA_G
Cuts at: 33 571 741 1201 1210 1425 1803 2189 2316
Size: 538 170 460 9 215 378 386 127
Cuts at: 2316 2657 2719 2967 3652 4645 4836 33
Size: 341 62 248 685 993 191 1121
Fragments arranged by size:
1121 993 685 538 460 386 378 341
248 215 191 170 127 62 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 1357 1357
Size: 5924
SfoI GGC'GCC
Cuts at: 3356 3604 3356
Size: 248 5676
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
SgrAI Cr'CCGG_yG
Cuts at: 2495 2495
Size: 5924
SmaI CCC'GGG
Cuts at: 347 2946 347
Size: 2599 3325
SmlI C'TyrA_G
Cuts at: 1501 2212 2485 3079 3994 4486 4748 1501
Size: 711 273 594 915 492 262 2677
Fragments arranged by size:
2677 915 711 594 492 273 262
SnaBI TAC'GTA
Cuts at: 2916 2916
Size: 5924
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5924
SphI G_CATG'C
Cuts at: 2977 3477 2977
Size: 500 5424
StuI AGG'CCT
Cuts at: 340 1372 1567 340
Size: 1032 195 4697
Fragments arranged by size:
4697 1032 195
StyI C'CwwG_G
Cuts at: 125 1061 1268 1377 1491 1511 2078 2726 3058
Size: 936 207 109 114 20 567 648 332
Cuts at: 3058 3412 125
Size: 354 2637
Fragments arranged by size:
2637 936 648 567 354 332 207 114
109 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 466 895 1238 2809 3507 3640 4099 4308 466
Size: 429 343 1571 698 133 459 209 2082
Fragments arranged by size:
2082 1571 698 459 429 343 209 133
TatI w'GTAC_w
Cuts at: 1940 5439 1940
Size: 3499 2425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 763 795 5441 5473 763
Size: 32 4646 32 1214
Fragments arranged by size:
4646 1214 32 32
Tth111I GACn'n_nGTC
Cuts at: 3721 3721
Size: 5924
XbaI T'CTAG_A
Cuts at: 2955 2955
Size: 5924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3419 3419
Size: 5924
XhoI C'TCGA_G
Cuts at: 2212 2212
Size: 5924
XmaI C'CCGG_G
Cuts at: 345 2944 345
Size: 2599 3325
XmnI GAAnn'nnTTC
Cuts at: 823 823
Size: 5924
ZraI GAC'GTC
Cuts at: 1827 1827
Size: 5924
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsrGI BssHII Bsu36I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SrfI SspI SwaI