Restriction map for
pF1KSDA0123
(Circular) (Six-base) MAPSORT of: pF1KSDA0123.seq Check: 9650 from: 1 to: 4712
pF1KSDA0123 4712 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1470 1470
Size: 4712
AccI GT'mk_AC
Cuts at: 481 1750 481
Size: 1269 3443
Acc65I G'GTAC_C
Cuts at: 1728 1728
Size: 4712
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2969 3716 3984 2537
Size: 432 747 268 3265
Fragments arranged by size:
3265 747 432 268
AflIII A'CryG_T
Cuts at: 1034 2064 3168 4145 1034
Size: 1030 1104 977 1601
Fragments arranged by size:
1601 1104 1030 977
AgeI A'CCGG_T
Cuts at: 3100 3100
Size: 4712
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1429 1461 1429
Size: 32 4680
AlwNI CAG_nnn'CTG
Cuts at: 1485 2017 3584 1485
Size: 532 1567 2613
Fragments arranged by size:
2613 1567 532
ApaI G_GGCC'C
Cuts at: 1057 1190 1057
Size: 133 4579
ApaLI G'TGCA_C
Cuts at: 1521 3482 1521
Size: 1961 2751
ApoI r'AATT_y
Cuts at: 398 509 1716 4065 4321 398
Size: 111 1207 2349 256 789
Fragments arranged by size:
2349 1207 789 256 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 4712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 168 1069 1732 168
Size: 901 663 3148
Fragments arranged by size:
3148 901 663
BamHI G'GATC_C
Cuts at: 1737 1737
Size: 4712
BanI G'GyrC_C
Cuts at: 235 1235 1670 1728 2142 2390 2425 235
Size: 1000 435 58 414 248 35 2522
Fragments arranged by size:
2522 1000 435 414 248 58 35
BanII G_rGCy'C
Cuts at: 169 1057 1076 1190 1726 2756 169
Size: 888 19 114 536 1030 2125
Fragments arranged by size:
2125 1030 888 536 114 19
BbeI G_GCGC'C
Cuts at: 1674 2146 2394 1674
Size: 472 248 3992
Fragments arranged by size:
3992 472 248
BbvCI CC'TCA_GC
Cuts at: 193 193
Size: 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 138 172 138
Size: 34 4678
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 2232 2232
Size: 4712
BglI GCCn_nnn'nGGC
Cuts at: 625 625
Size: 4712
BglII A'GATC_T
Cuts at: 988 2227 4264 988
Size: 1239 2037 1436
Fragments arranged by size:
2037 1436 1239
BlpI GC'TnA_GC
Cuts at: 1824 1824
Size: 4712
Bme1580I G_kGCm'C
Cuts at: 927 1057 1190 1525 2337 2430 3486 927
Size: 130 133 335 812 93 1056 2153
Fragments arranged by size:
2153 1056 812 335 133 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 993 1016 1924 2005 2340 4484 993
Size: 23 908 81 335 2144 1221
Fragments arranged by size:
2144 1221 908 335 81 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1122 3079 1122
Size: 1957 2755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 721 1117 1135 1191 1482 293
Size: 428 396 18 56 291 3523
Fragments arranged by size:
3523 428 396 291 56 18
Bpu10I CC'TnA_GC
Cuts at: 193 193
Size: 4712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2767 3259 3557 1888
Size: 879 492 298 3043
Fragments arranged by size:
3043 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4416 4416
Size: 4712
BsaAI yAC'GTr
Cuts at: 1631 1704 2065 1631
Size: 73 361 4278
Fragments arranged by size:
4278 361 73
BsaBI GATnn'nnATC
Cuts at: 6 2226 4263 6
Size: 2220 2037 455
Fragments arranged by size:
2220 2037 455
BsaHI Gr'CG_yC
Cuts at: 1671 2143 2391 3093 4589 1671
Size: 472 248 702 1496 1794
Fragments arranged by size:
1794 1496 702 472 248
BsaWI w'CCGG_w
Cuts at: 1697 1901 2422 3100 3374 3521 3932 1697
Size: 204 521 678 274 147 411 2477
Fragments arranged by size:
2477 678 521 411 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1243 1273 1243
Size: 30 4682
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1132 1132
Size: 4712
BseYI C'CCAG_C
Cuts at: 2137 2895 3472 3968 2137
Size: 758 577 496 2881
Fragments arranged by size:
2881 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 873 2254 2300 3508 104
Size: 769 1381 46 1208 1308
Fragments arranged by size:
1381 1308 1208 769 46
BsiHKAI G_wGCw'C
Cuts at: 905 1076 1229 1525 1640 1726 2504 2694 3486
Size: 171 153 296 115 86 778 190 792
Cuts at: 3486 905
Size: 2131
Fragments arranged by size:
2131 792 778 296 190 171 153 115
86
BsmI GAATG_Cn'
Cuts at: 840 840
Size: 4712
Bsp1286I G_dGCh'C
Cuts at: 169 905 927 1057 1076 1190 1229 1525 1640
Size: 736 22 130 19 114 39 296 115
Cuts at: 1640 1726 2337 2430 2504 2694 2756 3486 169
Size: 86 611 93 74 190 62 730 1395
Fragments arranged by size:
1395 736 730 611 296 190 130 115
114 93 86 74 62 39 22 19
BspEI T'CCGG_A
Cuts at: 3932 3932
Size: 4712
BspHI T'CATG_A
Cuts at: 753 1430 3888 753
Size: 677 2458 1577
Fragments arranged by size:
2458 1577 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 722 1470 1631 1762 1976 2278 2659 722
Size: 748 161 131 214 302 381 2775
Fragments arranged by size:
2775 748 381 302 214 161 131
BsrBI CCG'CTC
Cuts at: 50 3004 3058 4542 4707 50
Size: 2954 54 1484 165 55
Fragments arranged by size:
2954 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2624 2624
Size: 4712
BsrFI r'CCGG_y
Cuts at: 886 951 1590 1688 2710 2891 3100 886
Size: 65 639 98 1022 181 209 2498
Fragments arranged by size:
2498 1022 639 209 181 98 65
BsrGI T'GTAC_A
Cuts at: 388 388
Size: 4712
BssSI C'ACGA_G
Cuts at: 904 1513 2983 3341 904
Size: 609 1470 358 2275
Fragments arranged by size:
2275 1470 609 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2089 2089
Size: 4712
BstBI TT'CG_AA
Cuts at: 110 3073 110
Size: 2963 1749
BstEII G'GTnAC_C
Cuts at: 1609 1609
Size: 4712
BstXI CCAn_nnnn'nTGG
Cuts at: 1006 1006
Size: 4712
BstYI r'GATC_y
Cuts at: 1 988 1737 2204 2227 2562 2808 3820 3906
Size: 987 749 467 23 335 246 1012 86
Cuts at: 3906 3918 4264 1
Size: 12 346 449
Fragments arranged by size:
1012 987 749 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 850 850
Size: 4712
BtgI C'CryG_G
Cuts at: 125 974 1379 125
Size: 849 405 3458
Fragments arranged by size:
3458 849 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2662 2843 3006 2662
Size: 181 163 4368
Fragments arranged by size:
4368 181 163
BtsI GCAGTG_nn'
Cuts at: 1145 1601 1955 1145
Size: 456 354 3902
Fragments arranged by size:
3902 456 354
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2418 3276 1653
Size: 765 858 3089
Fragments arranged by size:
3089 858 765
EaeI y'GGCC_r
Cuts at: 884 1487 1598 2297 2471 2862 2889 4234 884
Size: 603 111 699 174 391 27 1345 1362
Fragments arranged by size:
1362 1345 699 603 391 174 111 27
EagI C'GGCC_G
Cuts at: 2297 2297
Size: 4712
EarI CTCTTCn'nnn_
Cuts at: 264 1552 1699 2735 2945 264
Size: 1288 147 1036 210 2031
Fragments arranged by size:
2031 1288 1036 210 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1175 1388 2920 3230 3376 4522 1175
Size: 213 1532 310 146 1146 1365
Fragments arranged by size:
1532 1365 1146 310 213 146
EcoICRI GAG'CTC
Cuts at: 1074 1724 1074
Size: 650 4062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 721 1117 1135 1191 1482 2537 2969 3716
Size: 428 396 18 56 291 1055 432 747
Cuts at: 3716 3984 293
Size: 268 1021
Fragments arranged by size:
1055 1021 747 432 428 396 291 268
56 18
EcoNI CCTnn'n_nnAGG
Cuts at: 849 1117 849
Size: 268 4444
EcoO109I rG'GnC_Cy
Cuts at: 705 1851 705
Size: 1146 3566
EcoRI G'AATT_C
Cuts at: 398 1716 398
Size: 1318 3394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4712
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 1313 1674 2146 2394 3416 4395 1313
Size: 361 472 248 1022 979 1630
Fragments arranged by size:
1630 1022 979 472 361 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2554 2584 2586 2616 2554
Size: 30 2 30 4650
Fragments arranged by size:
4650 30 30 2
HincII GTy'rAC
Cuts at: 1751 1751
Size: 4712
KasI G'GCGC_C
Cuts at: 1670 2142 2390 1670
Size: 472 248 3992
Fragments arranged by size:
3992 472 248
KpnI G_GTAC'C
Cuts at: 1732 1732
Size: 4712
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 1071 3383 3567 503
Size: 568 2312 184 1648
Fragments arranged by size:
2312 1648 568 184
MscI TGG'CCA
Cuts at: 1489 2473 1489
Size: 984 3728
MslI CAynn'nnrTG
Cuts at: 571 745 1153 2701 2828 571
Size: 174 408 1548 127 2455
Fragments arranged by size:
2455 1548 408 174 127
MspA1I CmG'CkG
Cuts at: 1416 1823 2137 2497 3510 3755 1416
Size: 407 314 360 1013 245 2373
Fragments arranged by size:
2373 1013 407 360 314 245
NaeI GCC'GGC
Cuts at: 888 953 1592 2893 888
Size: 65 639 1301 2707
Fragments arranged by size:
2707 1301 639 65
NarI GG'CG_CC
Cuts at: 1671 2143 2391 1671
Size: 472 248 3992
Fragments arranged by size:
3992 472 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4712
NgoMIV G'CCGG_C
Cuts at: 886 951 1590 2891 886
Size: 65 639 1301 2707
Fragments arranged by size:
2707 1301 639 65
NspI r_CATG'y
Cuts at: 1038 1203 1765 2265 3172 1038
Size: 165 562 500 907 2578
Fragments arranged by size:
2578 907 562 500 165
PasI CC'CwG_GG
Cuts at: 1605 1605
Size: 4712
PciI A'CATG_T
Cuts at: 1034 3168 1034
Size: 2134 2578
PfoI T'CCnGG_A
Cuts at: 1112 1112
Size: 4712
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PpuMI rG'GwC_Cy
Cuts at: 705 705
Size: 4712
PspOMI G'GGCC_C
Cuts at: 1053 1186 1053
Size: 133 4579
PstI C_TGCA'G
Cuts at: 644 1759 2444 644
Size: 1115 685 2912
Fragments arranged by size:
2912 1115 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 1416 2137 2497 1416
Size: 721 360 3631
Fragments arranged by size:
3631 721 360
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 1076 1726 1076
Size: 650 4062
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 1699 2735 2945 1699
Size: 1036 210 3466
Fragments arranged by size:
3466 1036 210
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 4229 4229
Size: 4712
SfcI C'TryA_G
Cuts at: 33 296 640 1755 2440 3433 3624 33
Size: 263 344 1115 685 993 191 1121
Fragments arranged by size:
1121 1115 993 685 344 263 191
SfoI GGC'GCC
Cuts at: 1672 2144 2392 1672
Size: 472 248 3992
Fragments arranged by size:
3992 472 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 1734 1734
Size: 4712
SmlI C'TyrA_G
Cuts at: 1867 2782 3274 3536 1867
Size: 915 492 262 3043
Fragments arranged by size:
3043 915 492 262
SnaBI TAC'GTA
Cuts at: 1704 1704
Size: 4712
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
StuI AGG'CCT
Cuts at: 1452 1452
Size: 4712
StyI C'CwwG_G
Cuts at: 125 1846 2200 125
Size: 1721 354 2637
Fragments arranged by size:
2637 1721 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1597 2295 2428 2887 3096 1597
Size: 698 133 459 209 3213
Fragments arranged by size:
3213 698 459 209 133
TatI w'GTAC_w
Cuts at: 388 1001 4227 388
Size: 613 3226 873
Fragments arranged by size:
3226 873 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 4680
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1047 2207 132
Size: 915 1160 2637
Fragments arranged by size:
2637 1160 915
XmaI C'CCGG_G
Cuts at: 1732 1732
Size: 4712
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsgI BsiWI BsmBI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI