Restriction map for
pF1KSDA0126
(Circular) (Six-base) MAPSORT of: pF1KSDA0126.seq Check: 3160 from: 1 to: 6356
pF1KSDA0126 6356 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1899 1899
Size: 6356
AccI GT'mk_AC
Cuts at: 3394 3394
Size: 6356
Acc65I G'GTAC_C
Cuts at: 2710 3372 2710
Size: 662 5694
AclI AA'CG_TT
Cuts at: 6196 6196
Size: 6356
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1321 1495 2832 4181 4613 5360 5628 253
Size: 1068 174 1337 1349 432 747 268 981
Fragments arranged by size:
1349 1337 1068 981 747 432 268 174
AflIII A'CryG_T
Cuts at: 2265 2408 3708 4812 5789 2265
Size: 143 1300 1104 977 2832
Fragments arranged by size:
2832 1300 1104 977 143
AgeI A'CCGG_T
Cuts at: 4744 4744
Size: 6356
AleI CACnn'nnGTG
Cuts at: 3147 3147
Size: 6356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 744 1391 744
Size: 647 5709
AlwNI CAG_nnn'CTG
Cuts at: 1299 3661 5228 1299
Size: 2362 1567 2427
Fragments arranged by size:
2427 2362 1567
ApaLI G'TGCA_C
Cuts at: 5126 5126
Size: 6356
ApoI r'AATT_y
Cuts at: 304 2023 2335 2469 2854 2925 3360 5709 5965
Size: 1719 312 134 385 71 435 2349 256
Cuts at: 5965 304
Size: 695
Fragments arranged by size:
2349 1719 695 435 385 312 256 134
71
AseI AT'TA_AT
Cuts at: 20 20
Size: 6356
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6356
AvaI C'yCGr_G
Cuts at: 3376 3376
Size: 6356
BamHI G'GATC_C
Cuts at: 3126 3381 3126
Size: 255 6101
BanI G'GyrC_C
Cuts at: 2710 2827 3372 3786 4034 4069 2710
Size: 117 545 414 248 35 4997
Fragments arranged by size:
4997 545 414 248 117 35
BanII G_rGCy'C
Cuts at: 846 1001 1960 3370 4400 846
Size: 155 959 1410 1030 2802
Fragments arranged by size:
2802 1410 1030 959 155
BbeI G_GCGC'C
Cuts at: 3790 4038 3790
Size: 248 6108
BbsI GAAGACnn'nnnn_
Cuts at: 1132 1132
Size: 6356
BciVI GTATCCnnnnn_n'
Cuts at: 2253 2402 4254 5015 6356 2253
Size: 149 1852 761 1341 2253
Fragments arranged by size:
2253 1852 1341 761 149
BclI T'GATC_A
Cuts at: 1725 3876 1725
Size: 2151 4205
BfrBI ATG'CAT
Cuts at: 1021 1021
Size: 6356
BglII A'GATC_T
Cuts at: 779 863 990 1286 3871 5908 779
Size: 84 127 296 2585 2037 1227
Fragments arranged by size:
2585 2037 1227 296 127 84
BlpI GC'TnA_GC
Cuts at: 1784 2768 3468 1784
Size: 984 700 4672
Fragments arranged by size:
4672 984 700
Bme1580I G_kGCm'C
Cuts at: 1545 2945 3981 4074 5130 1545
Size: 1400 1036 93 1056 2771
Fragments arranged by size:
2771 1400 1056 1036 93
BmrI ACTGGGnnnn_n'
Cuts at: 3568 3649 3984 6128 3568
Size: 81 335 2144 3796
Fragments arranged by size:
3796 2144 335 81
BmtI G_CTAG'C
Cuts at: 263 263
Size: 6356
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4723 4723
Size: 6356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1307 2128 2606 1297
Size: 10 821 478 5047
Fragments arranged by size:
5047 821 478 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 1531 3532 4411 4903 5201 141
Size: 1390 2001 879 492 298 1296
Fragments arranged by size:
2001 1390 1296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2339 6060 2339
Size: 3721 2635
BsaAI yAC'GTr
Cuts at: 3348 3709 3348
Size: 361 5995
BsaBI GATnn'nnATC
Cuts at: 6 1843 2426 3870 5907 6
Size: 1837 583 1444 2037 455
Fragments arranged by size:
2037 1837 1444 583 455
BsaHI Gr'CG_yC
Cuts at: 3787 4035 4737 6233 3787
Size: 248 702 1496 3910
Fragments arranged by size:
3910 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2258 3545 4066 4744 5018 5165 5576 2258
Size: 1287 521 678 274 147 411 3038
Fragments arranged by size:
3038 1287 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 1627 1657 2483 2513 643
Size: 30 954 30 826 30 4486
Fragments arranged by size:
4486 954 826 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1507 2935 1507
Size: 1428 4928
BseYI C'CCAG_C
Cuts at: 255 427 1134 1623 2870 3781 4539 5116 5612
Size: 172 707 489 1247 911 758 577 496
Cuts at: 5612 255
Size: 999
Fragments arranged by size:
1247 999 911 758 707 577 496 489
172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 750 1651 2877 745
Size: 5 901 1226 4224
Fragments arranged by size:
4224 1226 901 5
BsiEI CG_ry'CG
Cuts at: 104 3898 3944 5152 104
Size: 3794 46 1208 1308
Fragments arranged by size:
3794 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1001 3142 3370 4148 4338 5130 1001
Size: 2141 228 778 190 792 2227
Fragments arranged by size:
2227 2141 792 778 228 190
Bsp1286I G_dGCh'C
Cuts at: 846 1001 1545 1960 2945 3142 3370 3981 4074
Size: 155 544 415 985 197 228 611 93
Cuts at: 4074 4148 4338 4400 5130 846
Size: 74 190 62 730 2072
Fragments arranged by size:
2072 985 730 611 544 415 228 197
190 155 93 74 62
BspEI T'CCGG_A
Cuts at: 5576 5576
Size: 6356
BspHI T'CATG_A
Cuts at: 950 5532 950
Size: 4582 1774
BspMI ACCTGCnnnn'nnnn_
Cuts at: 716 1755 1899 2522 3406 3620 3922 4303 716
Size: 1039 144 623 884 214 302 381 2769
Fragments arranged by size:
2769 1039 884 623 381 302 214 144
BsrBI CCG'CTC
Cuts at: 50 335 4648 4702 6186 6351 50
Size: 285 4313 54 1484 165 55
Fragments arranged by size:
4313 1484 285 165 55 54
BsrDI GCAATG_nn'
Cuts at: 528 1956 3200 4268 528
Size: 1428 1244 1068 2616
Fragments arranged by size:
2616 1428 1244 1068
BsrFI r'CCGG_y
Cuts at: 4354 4535 4744 4354
Size: 181 209 5966
Fragments arranged by size:
5966 209 181
BsrGI T'GTAC_A
Cuts at: 1584 1584
Size: 6356
BssSI C'ACGA_G
Cuts at: 4627 4985 4627
Size: 358 5998
BstAPI GCAn_nnn'nTGC
Cuts at: 1761 3733 1761
Size: 1972 4384
BstBI TT'CG_AA
Cuts at: 110 4717 110
Size: 4607 1749
BstYI r'GATC_y
Cuts at: 1 395 561 779 863 990 1007 1286 1504
Size: 394 166 218 84 127 17 279 218
Cuts at: 1504 3126 3193 3231 3266 3381 3848 3871 4206
Size: 1622 67 38 35 115 467 23 335
Cuts at: 4206 4452 5464 5550 5562 5908 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1622 1012 467 449 394 346 335 279
246 218 218 166 127 115 86 84
67 38 35 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2173 2173
Size: 6356
BtgI C'CryG_G
Cuts at: 1916 3257 1916
Size: 1341 5015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4306 4487 4650 4306
Size: 181 163 6012
Fragments arranged by size:
6012 181 163
BtsI GCAGTG_nn'
Cuts at: 728 733 2210 3599 728
Size: 5 1477 1389 3485
Fragments arranged by size:
3485 1477 1389 5
DraI TTT'AAA
Cuts at: 3355 3355
Size: 6356
DrdI GACnn_nn'nnGTC
Cuts at: 4062 4920 4062
Size: 858 5498
EaeI y'GGCC_r
Cuts at: 1940 3271 3941 4115 4506 4533 5878 1940
Size: 1331 670 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 1331 670 391 174 27
EagI C'GGCC_G
Cuts at: 3941 3941
Size: 6356
EarI CTCTTCn'nnn_
Cuts at: 544 677 890 3083 4379 4589 544
Size: 133 213 2193 1296 210 2311
Fragments arranged by size:
2311 2193 1296 213 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 4564 4874 5020 6166 2053
Size: 2511 310 146 1146 2243
Fragments arranged by size:
2511 2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 999 3368 999
Size: 2369 3987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1297 1307 1321 1495 2128 2606 2832 4181
Size: 1044 10 14 174 633 478 226 1349
Cuts at: 4181 4613 5360 5628 253
Size: 432 747 268 981
Fragments arranged by size:
1349 1044 981 747 633 478 432 268
226 174 14 10
EcoO109I rG'GnC_Cy
Cuts at: 534 1098 3495 534
Size: 564 2397 3395
Fragments arranged by size:
3395 2397 564
EcoRI G'AATT_C
Cuts at: 304 3360 304
Size: 3056 3300
EcoRV GAT'ATC
Cuts at: 2889 2889
Size: 6356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 526 2763 4375 526
Size: 2237 1612 2507
Fragments arranged by size:
2507 2237 1612
FspI TGC'GCA
Cuts at: 4137 4137
Size: 6356
HaeII r_GCGC'y
Cuts at: 3790 4038 5060 6039 3790
Size: 248 1022 979 4107
Fragments arranged by size:
4107 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1833 1865 2217 2249 2446 2478 2725 2757 3200
Size: 32 352 32 197 32 247 32 443
Cuts at: 3200 3232 4198 4228 4230 4260 1833
Size: 32 966 30 2 30 3929
Fragments arranged by size:
3929 966 443 352 247 197 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 789 3395 789
Size: 2606 3750
KasI G'GCGC_C
Cuts at: 3786 4034 3786
Size: 248 6108
KpnI G_GTAC'C
Cuts at: 2714 3376 2714
Size: 662 5694
MluI A'CGCG_T
Cuts at: 5789 5789
Size: 6356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2177 3102 3324 5027 5211 2177
Size: 925 222 1703 184 3322
Fragments arranged by size:
3322 1703 925 222 184
MscI TGG'CCA
Cuts at: 1942 4117 1942
Size: 2175 4181
MslI CAynn'nnrTG
Cuts at: 1373 2782 3145 3147 4345 4472 1373
Size: 1409 363 2 1198 127 3257
Fragments arranged by size:
3257 1409 1198 363 127 2
MspA1I CmG'CkG
Cuts at: 231 1627 2670 3467 3781 4141 5154 5399 231
Size: 1396 1043 797 314 360 1013 245 1188
Fragments arranged by size:
1396 1188 1043 1013 797 360 314 245
NaeI GCC'GGC
Cuts at: 4537 4537
Size: 6356
NarI GG'CG_CC
Cuts at: 3787 4035 3787
Size: 248 6108
NcoI C'CATG_G
Cuts at: 1916 3257 1916
Size: 1341 5015
NgoMIV G'CCGG_C
Cuts at: 4535 4535
Size: 6356
NheI G'CTAG_C
Cuts at: 259 259
Size: 6356
NsiI A_TGCA'T
Cuts at: 1023 1023
Size: 6356
NspI r_CATG'y
Cuts at: 1372 2116 2412 3409 3909 4816 1372
Size: 744 296 997 500 907 2912
Fragments arranged by size:
2912 997 907 744 500 296
PacI TTA_AT'TAA
Cuts at: 581 581
Size: 6356
PciI A'CATG_T
Cuts at: 2408 4812 2408
Size: 2404 3952
PflMI CCAn_nnn'nTGG
Cuts at: 1394 1394
Size: 6356
PfoI T'CCnGG_A
Cuts at: 3122 3122
Size: 6356
PmeI GTTT'AAAC
Cuts at: 3355 3355
Size: 6356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5495 5527 5495
Size: 32 6324
PpuMI rG'GwC_Cy
Cuts at: 1098 1098
Size: 6356
PstI C_TGCA'G
Cuts at: 1766 3403 4088 1766
Size: 1637 685 4034
Fragments arranged by size:
4034 1637 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6356
PvuII CAG'CTG
Cuts at: 231 1627 2670 3781 4141 231
Size: 1396 1043 1111 360 2446
Fragments arranged by size:
2446 1396 1111 1043 360
RsrII CG'GwC_CG
Cuts at: 4551 4551
Size: 6356
SacI G_AGCT'C
Cuts at: 1001 3370 1001
Size: 2369 3987
SalI G'TCGA_C
Cuts at: 3393 3393
Size: 6356
SapI GCTCTTCn'nnn_
Cuts at: 4379 4589 4379
Size: 210 6146
SbfI CC_TGCA'GG
Cuts at: 3403 3403
Size: 6356
ScaI AGT'ACT
Cuts at: 5873 5873
Size: 6356
SexAI A'CCwGG_T
Cuts at: 2807 2807
Size: 6356
SfcI C'TryA_G
Cuts at: 33 1762 1998 2653 3399 4084 5077 5268 33
Size: 1729 236 655 746 685 993 191 1121
Fragments arranged by size:
1729 1121 993 746 685 655 236 191
SfoI GGC'GCC
Cuts at: 3788 4036 3788
Size: 248 6108
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6356
SmaI CCC'GGG
Cuts at: 3378 3378
Size: 6356
SmlI C'TyrA_G
Cuts at: 156 1546 3511 4426 4918 5180 156
Size: 1390 1965 915 492 262 1332
Fragments arranged by size:
1965 1390 1332 915 492 262
SnaBI TAC'GTA
Cuts at: 3348 3348
Size: 6356
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6356
SphI G_CATG'C
Cuts at: 1372 3409 3909 1372
Size: 2037 500 3819
Fragments arranged by size:
3819 2037 500
StyI C'CwwG_G
Cuts at: 677 1094 1916 2237 2945 3257 3490 3844 677
Size: 417 822 321 708 312 233 354 3189
Fragments arranged by size:
3189 822 708 417 354 321 312 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1450 2032 2815 3939 4072 4531 4740 1450
Size: 582 783 1124 133 459 209 3066
Fragments arranged by size:
3066 1124 783 582 459 209 133
TatI w'GTAC_w
Cuts at: 851 1239 1584 1691 1700 5871 851
Size: 388 345 107 9 4171 1336
Fragments arranged by size:
4171 1336 388 345 107 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1512 1544 2483 2515 5873 5905 1512
Size: 32 939 32 3358 32 1963
Fragments arranged by size:
3358 1963 939 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4153 4153
Size: 6356
XbaI T'CTAG_A
Cuts at: 993 3387 993
Size: 2394 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2538 3183 3851 2538
Size: 645 668 5043
Fragments arranged by size:
5043 668 645
XmaI C'CCGG_G
Cuts at: 3376 3376
Size: 6356
XmnI GAAnn'nnTTC
Cuts at: 1085 2794 1085
Size: 1709 4647
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PacI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI Bpu10I BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI
XhoI ZraI