Restriction map for
pF1KSDA0127
(Circular) (Six-base) MAPSORT of: pF1KSDA0127.seq Check: 2549 from: 1 to: 4081
pF1KSDA0127 4081 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 826 826
Size: 4081
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4081
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4081
AclI AA'CG_TT
Cuts at: 3921 3921
Size: 4081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 1904 2336 3085 3353 429
Size: 1475 432 749 268 1157
Fragments arranged by size:
1475 1157 749 432 268
AflIII A'CryG_T
Cuts at: 326 548 1431 2537 3514 326
Size: 222 883 1106 977 893
Fragments arranged by size:
1106 977 893 883 222
AgeI A'CCGG_T
Cuts at: 2469 2469
Size: 4081
AlwNI CAG_nnn'CTG
Cuts at: 1384 2953 1384
Size: 1569 2512
ApaLI G'TGCA_C
Cuts at: 2851 2851
Size: 4081
ApoI r'AATT_y
Cuts at: 807 1083 3434 3690 807
Size: 276 2351 256 1198
Fragments arranged by size:
2351 1198 276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4081
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4081
AvaI C'yCGr_G
Cuts at: 532 1099 532
Size: 567 3514
BamHI G'GATC_C
Cuts at: 338 1104 338
Size: 766 3315
BanI G'GyrC_C
Cuts at: 736 1095 1509 1757 1792 736
Size: 359 414 248 35 3025
Fragments arranged by size:
3025 414 359 248 35
BanII G_rGCy'C
Cuts at: 296 413 662 1093 2123 296
Size: 117 249 431 1030 2254
Fragments arranged by size:
2254 1030 431 249 117
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3833
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2740 4081 1977
Size: 763 1341 1977
Fragments arranged by size:
1977 1341 763
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4081
BglI GCCn_nnn'nGGC
Cuts at: 439 439
Size: 4081
BglII A'GATC_T
Cuts at: 1594 3633 1594
Size: 2039 2042
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4081
Bme1580I G_kGCm'C
Cuts at: 1704 1797 2855 1704
Size: 93 1058 2930
Fragments arranged by size:
2930 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1372 1707 3853 1291
Size: 81 335 2146 1519
Fragments arranged by size:
2146 1519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 587 708 523
Size: 64 121 3896
Fragments arranged by size:
3896 121 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 1255 2134 2628 2926 743
Size: 512 879 494 298 1898
Fragments arranged by size:
1898 879 512 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3785 3785
Size: 4081
BsaAI yAC'GTr
Cuts at: 1071 1432 1071
Size: 361 3720
BsaBI GATnn'nnATC
Cuts at: 6 1593 3632 6
Size: 1587 2039 455
Fragments arranged by size:
2039 1587 455
BsaHI Gr'CG_yC
Cuts at: 457 823 1510 1758 2462 3958 457
Size: 366 687 248 704 1496 580
Fragments arranged by size:
1496 704 687 580 366 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2469 2743 2890 3301 1268
Size: 521 680 274 147 411 2048
Fragments arranged by size:
2048 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 616 646 960 990 616
Size: 30 314 30 3707
Fragments arranged by size:
3707 314 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 381 624 670 952 381
Size: 243 46 282 3510
Fragments arranged by size:
3510 282 243 46
BseYI C'CCAG_C
Cuts at: 295 395 412 1504 2262 2841 3337 295
Size: 100 17 1092 758 579 496 1039
Fragments arranged by size:
1092 1039 758 579 496 100 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 891
Size: 4081
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2877 104
Size: 1517 46 1210 1308
Fragments arranged by size:
1517 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 662 1093 1871 2061 2855 662
Size: 431 778 190 794 1888
Fragments arranged by size:
1888 794 778 431 190
Bsp1286I G_dGCh'C
Cuts at: 296 413 662 1093 1704 1797 1871 2061 2123
Size: 117 249 431 611 93 74 190 62
Cuts at: 2123 2855 296
Size: 732 1522
Fragments arranged by size:
1522 732 611 431 249 190 117 93
74 62
BspEI T'CCGG_A
Cuts at: 3301 3301
Size: 4081
BspHI T'CATG_A
Cuts at: 3257 3257
Size: 4081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3184
Fragments arranged by size:
3184 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3911 4076 50
Size: 2321 54 1486 165 55
Fragments arranged by size:
2321 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4081
BsrFI r'CCGG_y
Cuts at: 438 2077 2258 2469 438
Size: 1639 181 211 2050
Fragments arranged by size:
2050 1639 211 181
BssSI C'ACGA_G
Cuts at: 2350 2710 2350
Size: 360 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 1456
Size: 4081
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4081
BstYI r'GATC_y
Cuts at: 1 338 1104 1571 1594 1929 2175 3189 3275
Size: 337 766 467 23 335 246 1014 86
Cuts at: 3275 3287 3633 1
Size: 12 346 449
Fragments arranged by size:
1014 766 467 449 346 337 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 370 370
Size: 4081
BtgI C'CryG_G
Cuts at: 578 692 827 578
Size: 114 135 3832
Fragments arranged by size:
3832 135 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3737
Fragments arranged by size:
3737 181 163
BtsI GCAGTG_nn'
Cuts at: 526 1322 526
Size: 796 3285
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4081
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2645 1785
Size: 860 3221
EaeI y'GGCC_r
Cuts at: 689 1664 1838 2229 2256 3603 689
Size: 975 174 391 27 1347 1167
Fragments arranged by size:
1347 1167 975 391 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4081
EarI CTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3871
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 351 2287 2599 2745 3891 351
Size: 1936 312 146 1146 541
Fragments arranged by size:
1936 1146 541 312 146
EcoICRI GAG'CTC
Cuts at: 660 1091 660
Size: 431 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 523 587 708 1904 2336 3085 3353 429
Size: 94 64 121 1196 432 749 268 1157
Fragments arranged by size:
1196 1157 749 432 268 121 94 64
EcoO109I rG'GnC_Cy
Cuts at: 281 432 719 1064 1218 281
Size: 151 287 345 154 3144
Fragments arranged by size:
3144 345 287 154 151
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4081
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1028 2098 1028
Size: 1070 3011
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4081
HaeII r_GCGC'y
Cuts at: 237 1513 1761 2785 3764 237
Size: 1276 248 1024 979 554
Fragments arranged by size:
1276 1024 979 554 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 616 648 780 812 1921 1951 1953
Size: 32 391 32 132 32 1109 30 2
Cuts at: 1953 1983 193
Size: 30 2291
Fragments arranged by size:
2291 1109 391 132 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4081
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3833
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4081
MluI A'CGCG_T
Cuts at: 3514 3514
Size: 4081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2752 2936 2752
Size: 184 3897
MscI TGG'CCA
Cuts at: 1840 1840
Size: 4081
MslI CAynn'nnrTG
Cuts at: 167 1051 2068 2195 167
Size: 884 1017 127 2053
Fragments arranged by size:
2053 1017 884 127
MspA1I CmG'CkG
Cuts at: 1190 1504 1864 2879 3124 1190
Size: 314 360 1015 245 2147
Fragments arranged by size:
2147 1015 360 314 245
NaeI GCC'GGC
Cuts at: 440 2260 440
Size: 1820 2261
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3833
NgoMIV G'CCGG_C
Cuts at: 438 2258 438
Size: 1820 2261
NruI TCG'CGA
Cuts at: 2442 2442
Size: 4081
NspI r_CATG'y
Cuts at: 330 1132 1632 2541 330
Size: 802 500 909 1870
Fragments arranged by size:
1870 909 802 500
PacI TTA_AT'TAA
Cuts at: 320 320
Size: 4081
PciI A'CATG_T
Cuts at: 326 2537 326
Size: 2211 1870
PflMI CCAn_nnn'nTGG
Cuts at: 1047 1047
Size: 4081
PfoI T'CCnGG_A
Cuts at: 341 341
Size: 4081
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3252 3220
Size: 32 4049
PpuMI rG'GwC_Cy
Cuts at: 719 1064 719
Size: 345 3736
PspXI vC'TCGA_Gb
Cuts at: 532 532
Size: 4081
PstI C_TGCA'G
Cuts at: 1126 1811 1126
Size: 685 3396
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4081
PvuII CAG'CTG
Cuts at: 1504 1864 1504
Size: 360 3721
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4081
SacI G_AGCT'C
Cuts at: 662 1093 662
Size: 431 3650
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4081
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3871
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4081
ScaI AGT'ACT
Cuts at: 3598 3598
Size: 4081
SfcI C'TryA_G
Cuts at: 33 1122 1807 2802 2993 33
Size: 1089 685 995 191 1121
Fragments arranged by size:
1121 1089 995 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 439 439
Size: 4081
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3833
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4081
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4081
SmlI C'TyrA_G
Cuts at: 532 758 1234 2149 2643 2905 532
Size: 226 476 915 494 262 1708
Fragments arranged by size:
1708 915 494 476 262 226
SnaBI TAC'GTA
Cuts at: 1071 1071
Size: 4081
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4081
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3581
StuI AGG'CCT
Cuts at: 508 508
Size: 4081
StyI C'CwwG_G
Cuts at: 212 641 1213 1567 212
Size: 429 572 354 2726
Fragments arranged by size:
2726 572 429 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 1662 1795 2254 2465 754
Size: 908 133 459 211 2370
Fragments arranged by size:
2370 908 459 211 133
TatI w'GTAC_w
Cuts at: 3596 3596
Size: 4081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 442 474 3598 3630 442
Size: 32 3124 32 893
Fragments arranged by size:
3124 893 32 32
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4081
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1574 1574
Size: 4081
XhoI C'TCGA_G
Cuts at: 532 532
Size: 4081
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4081
ZraI GAC'GTC
Cuts at: 824 824
Size: 4081
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI
PacI PciI PflMI PfoI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NsiI PasI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SgrAI SrfI SspI SwaI XmnI