Restriction map for
pF1KSDA0135
(Circular) (Six-base) MAPSORT of: pF1KSDA0135.seq Check: 1875 from: 1 to: 7129
pF1KSDA0135 7129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 1358 1358
Size: 7129
AccI GT'mk_AC
Cuts at: 2847 4165 2847
Size: 1318 5811
Acc65I G'GTAC_C
Cuts at: 1042 4143 1042
Size: 3101 4028
AclI AA'CG_TT
Cuts at: 6969 6969
Size: 7129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 1793 4952 5384 6133 6401 341
Size: 1452 3159 432 749 268 1069
Fragments arranged by size:
3159 1452 1069 749 432 268
AfeI AGC'GCT
Cuts at: 314 314
Size: 7129
AflIII A'CryG_T
Cuts at: 914 2661 2696 4479 5585 6562 914
Size: 1747 35 1783 1106 977 1481
Fragments arranged by size:
1783 1747 1481 1106 977 35
AgeI A'CCGG_T
Cuts at: 1102 5517 1102
Size: 4415 2714
AhdI GACnn_n'nnGTC
Cuts at: 2388 2388
Size: 7129
AleI CACnn'nnGTG
Cuts at: 724 2749 724
Size: 2025 5104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 410 1676 410
Size: 1266 5863
AlwNI CAG_nnn'CTG
Cuts at: 296 609 652 766 1638 3047 3853 4432 6001
Size: 313 43 114 872 1409 806 579 1569
Cuts at: 6001 296
Size: 1424
Fragments arranged by size:
1569 1424 1409 872 806 579 313 114
43
ApaI G_GGCC'C
Cuts at: 211 2884 4048 211
Size: 2673 1164 3292
Fragments arranged by size:
3292 2673 1164
ApaLI G'TGCA_C
Cuts at: 1621 3653 5899 1621
Size: 2032 2246 2851
Fragments arranged by size:
2851 2246 2032
ApoI r'AATT_y
Cuts at: 2334 4131 6482 6738 2334
Size: 1797 2351 256 2725
Fragments arranged by size:
2725 2351 1797 256
AseI AT'TA_AT
Cuts at: 20 1442 20
Size: 1422 5707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7129
AvaI C'yCGr_G
Cuts at: 1475 4090 4147 1475
Size: 2615 57 4457
Fragments arranged by size:
4457 2615 57
BamHI G'GATC_C
Cuts at: 1431 3252 4095 4152 1431
Size: 1821 843 57 4408
Fragments arranged by size:
4408 1821 843 57
BanI G'GyrC_C
Cuts at: 868 1042 2530 2800 4143 4557 4805 4840 868
Size: 174 1488 270 1343 414 248 35 3157
Fragments arranged by size:
3157 1488 1343 414 270 248 174 35
BanII G_rGCy'C
Cuts at: 211 236 337 587 827 1253 1699 2148 2455
Size: 25 101 250 240 426 446 449 307
Cuts at: 2455 2876 2884 3008 3062 3136 3434 4048 4141
Size: 421 8 124 54 74 298 614 93
Cuts at: 4141 5171 211
Size: 1030 2169
Fragments arranged by size:
2169 1030 614 449 446 426 421 307
298 250 240 124 101 93 74 54
25 8
BbeI G_GCGC'C
Cuts at: 4561 4809 4561
Size: 248 6881
BbsI GAAGACnn'nnnn_
Cuts at: 329 329
Size: 7129
BbvCI CC'TCA_GC
Cuts at: 252 670 1418 1574 1790 2262 3557 252
Size: 418 748 156 216 472 1295 3824
Fragments arranged by size:
3824 1295 748 472 418 216 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2015 2049 3634 3668 2015
Size: 34 1585 34 5476
Fragments arranged by size:
5476 1585 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3490 5025 5788 7129 3490
Size: 1535 763 1341 3490
Fragments arranged by size:
3490 1535 1341 763
BclI T'GATC_A
Cuts at: 4647 4647
Size: 7129
BglI GCCn_nnn'nGGC
Cuts at: 2978 3140 2978
Size: 162 6967
BglII A'GATC_T
Cuts at: 4642 6681 4642
Size: 2039 5090
BlpI GC'TnA_GC
Cuts at: 230 3002 4239 230
Size: 2772 1237 3120
Fragments arranged by size:
3120 2772 1237
Bme1580I G_kGCm'C
Cuts at: 211 1475 1625 2704 2884 3657 4048 4752 4845
Size: 1264 150 1079 180 773 391 704 93
Cuts at: 4845 5903 211
Size: 1058 1437
Fragments arranged by size:
1437 1264 1079 1058 773 704 391 180
150 93
BmgBI CAC'GTC
Cuts at: 878 930 878
Size: 52 7077
BmrI ACTGGGnnnn_n'
Cuts at: 2121 4339 4420 4755 6901 2121
Size: 2218 81 335 2146 2349
Fragments arranged by size:
2349 2218 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 855 1570 2451 5494 855
Size: 715 881 3043 2490
Fragments arranged by size:
3043 2490 881 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1565 1568 1763 2461 2860 3100 3718 3784
Size: 724 3 195 698 399 240 618 66
Cuts at: 3784 4009 841
Size: 225 3961
Fragments arranged by size:
3961 724 698 618 399 240 225 195
66 3
Bpu10I CC'TnA_GC
Cuts at: 252 268 404 670 1418 1574 1790 2262 3557
Size: 16 136 266 748 156 216 472 1295
Cuts at: 3557 4028 252
Size: 471 3353
Fragments arranged by size:
3353 1295 748 472 471 266 216 156
136 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 2338 2623 4303 5182 5676 5974 991
Size: 1347 285 1680 879 494 298 2146
Fragments arranged by size:
2146 1680 1347 879 494 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 843 2225 3713 3763 6833 843
Size: 1382 1488 50 3070 1139
Fragments arranged by size:
3070 1488 1382 1139 50
BsaAI yAC'GTr
Cuts at: 915 2699 4119 4480 915
Size: 1784 1420 361 3564
Fragments arranged by size:
3564 1784 1420 361
BsaBI GATnn'nnATC
Cuts at: 6 4641 6680 6
Size: 4635 2039 455
Fragments arranged by size:
4635 2039 455
BsaHI Gr'CG_yC
Cuts at: 1355 4558 4806 5510 7006 1355
Size: 3203 248 704 1496 1478
Fragments arranged by size:
3203 1496 1478 704 248
BsaWI w'CCGG_w
Cuts at: 1102 1487 3010 3657 4316 4837 5517 5791 5938
Size: 385 1523 647 659 521 680 274 147
Cuts at: 5938 6349 1102
Size: 411 1882
Fragments arranged by size:
1882 1523 680 659 647 521 411 385
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2107 2137 3691 3721 3996 4026 2107
Size: 30 1554 30 275 30 5210
Fragments arranged by size:
5210 1554 275 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 1105 2250 2728 2911 3070 3757 3781 691
Size: 414 1145 478 183 159 687 24 4039
Fragments arranged by size:
4039 1145 687 478 414 183 159 24
BseYI C'CCAG_C
Cuts at: 210 235 934 1081 1413 1785 1838 1867 1888
Size: 25 699 147 332 372 53 29 21
Cuts at: 1888 2008 2067 3016 3138 4552 5310 5889 6385
Size: 120 59 949 122 1414 758 579 496
Cuts at: 6385 210
Size: 954
Fragments arranged by size:
1414 954 949 758 699 579 496 372
332 147 122 120 59 53 29 25
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 370 988 1137 2232 200
Size: 170 618 149 1095 5097
Fragments arranged by size:
5097 1095 618 170 149
BsiEI CG_ry'CG
Cuts at: 104 2085 3411 3596 4669 4715 5925 104
Size: 1981 1326 185 1073 46 1210 1308
Fragments arranged by size:
1981 1326 1308 1210 1073 185 46
BsiHKAI G_wGCw'C
Cuts at: 337 410 683 1253 1625 2876 3008 3035 3308
Size: 73 273 570 372 1251 132 27 273
Cuts at: 3308 3657 4141 4919 5109 5903 337
Size: 349 484 778 190 794 1563
Fragments arranged by size:
1563 1251 794 778 570 484 372 349
273 273 190 132 73 27
BsmBI CGTCTCn'nnnn_
Cuts at: 2822 3852 2822
Size: 1030 6099
Bsp1286I G_dGCh'C
Cuts at: 211 236 337 410 587 683 827 1253 1475
Size: 25 101 73 177 96 144 426 222
Cuts at: 1475 1625 1699 2148 2455 2704 2876 2884 3008
Size: 150 74 449 307 249 172 8 124
Cuts at: 3008 3035 3062 3136 3308 3434 3657 4048 4141
Size: 27 27 74 172 126 223 391 93
Cuts at: 4141 4752 4845 4919 5109 5171 5903 211
Size: 611 93 74 190 62 732 1437
Fragments arranged by size:
1437 732 611 449 426 391 307 249
223 222 190 177 172 172 150 144
126 124 101 96 93 93 74 74
74 73 62 27 27 25 8
BspEI T'CCGG_A
Cuts at: 1487 6349 1487
Size: 4862 2267
BspHI T'CATG_A
Cuts at: 3818 6305 3818
Size: 2487 4642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 1715 2110 3509 4177 4391 4693 5074 230
Size: 1485 395 1399 668 214 302 381 2285
Fragments arranged by size:
2285 1485 1399 668 395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1124 1848 2200 5419 5473 6959 7124 50
Size: 1074 724 352 3219 54 1486 165 55
Fragments arranged by size:
3219 1486 1074 724 352 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1368 5039 1368
Size: 3671 3458
BsrFI r'CCGG_y
Cuts at: 1102 2527 5125 5306 5517 1102
Size: 1425 2598 181 211 2714
Fragments arranged by size:
2714 2598 1425 211 181
BsrGI T'GTAC_A
Cuts at: 373 3725 373
Size: 3352 3777
BssSI C'ACGA_G
Cuts at: 5398 5758 5398
Size: 360 6769
BstAPI GCAn_nnn'nTGC
Cuts at: 4504 4504
Size: 7129
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7129
BstEII G'GTnAC_C
Cuts at: 2736 2736
Size: 7129
BstXI CCAn_nnnn'nTGG
Cuts at: 865 1350 3024 3072 865
Size: 485 1674 48 4922
Fragments arranged by size:
4922 1674 485 48
BstYI r'GATC_y
Cuts at: 1 554 1431 1484 1707 2789 3252 4095 4152
Size: 553 877 53 223 1082 463 843 57
Cuts at: 4152 4619 4642 4977 5223 6237 6323 6335 6681
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6681 1
Size: 449
Fragments arranged by size:
1082 1014 877 843 553 467 463 449
346 335 246 223 86 57 53 23
12
BstZ17I GTA'TAC
Cuts at: 2848 2848
Size: 7129
Bsu36I CC'TnA_GG
Cuts at: 471 2690 471
Size: 2219 4910
BtgI C'CryG_G
Cuts at: 125 427 827 1197 1396 1898 2062 2282 3773
Size: 302 400 370 199 502 164 220 1491
Cuts at: 3773 3892 125
Size: 119 3362
Fragments arranged by size:
3362 1491 502 400 370 302 220 199
164 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 878 5077 5258 5421 878
Size: 4199 181 163 2586
Fragments arranged by size:
4199 2586 181 163
BtsI GCAGTG_nn'
Cuts at: 224 387 441 1658 1809 1950 2315 2453 3150
Size: 163 54 1217 151 141 365 138 697
Cuts at: 3150 3497 4370 224
Size: 347 873 2983
Fragments arranged by size:
2983 1217 873 697 365 347 163 151
141 138 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1350 1385 1350
Size: 35 7094
DraI TTT'AAA
Cuts at: 4126 4126
Size: 7129
DraIII CAC_nnn'GTG
Cuts at: 1470 1470
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 4833 5693 4833
Size: 860 6269
EaeI y'GGCC_r
Cuts at: 615 696 984 1340 1461 1901 2279 2547 3593
Size: 81 288 356 121 440 378 268 1046
Cuts at: 3593 4071 4712 4886 5277 5304 6651 615
Size: 478 641 174 391 27 1347 1093
Fragments arranged by size:
1347 1093 1046 641 478 440 391 378
356 288 268 174 121 81 27
EagI C'GGCC_G
Cuts at: 3593 4712 3593
Size: 1119 6010
EarI CTCTTCn'nnn_
Cuts at: 149 772 1023 2084 2442 3400 3929 5150 5360
Size: 623 251 1061 358 958 529 1221 210
Cuts at: 5360 149
Size: 1918
Fragments arranged by size:
1918 1221 1061 958 623 529 358 251
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5335 5647 5793 6939 5335
Size: 312 146 1146 5525
Fragments arranged by size:
5525 1146 312 146
EcoICRI GAG'CTC
Cuts at: 335 1251 2874 3006 4139 335
Size: 916 1623 132 1133 3325
Fragments arranged by size:
3325 1623 1133 916 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 841 1565 1568 1763 1793 2461 2860 3100
Size: 500 724 3 195 30 668 399 240
Cuts at: 3100 3718 3784 4009 4952 5384 6133 6401 341
Size: 618 66 225 943 432 749 268 1069
Fragments arranged by size:
1069 943 749 724 668 618 500 432
399 268 240 225 195 66 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 673 1583 673
Size: 910 6219
EcoO109I rG'GnC_Cy
Cuts at: 207 606 666 1410 2298 2880 2881 2951 3149
Size: 399 60 744 888 582 1 70 198
Cuts at: 3149 4044 4045 4266 207
Size: 895 1 221 3070
Fragments arranged by size:
3070 895 888 744 582 399 221 198
70 60 1 1
EcoRI G'AATT_C
Cuts at: 2334 4131 2334
Size: 1797 5332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 543 2379 5146 543
Size: 1836 2767 2526
Fragments arranged by size:
2767 2526 1836
FspI TGC'GCA
Cuts at: 4908 4908
Size: 7129
HaeII r_GCGC'y
Cuts at: 316 798 1639 4561 4809 5833 6812 316
Size: 482 841 2922 248 1024 979 633
Fragments arranged by size:
2922 1024 979 841 633 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1469 1501 2034 2066 2107 2139 2374 2406 3237
Size: 32 533 32 41 32 235 32 831
Cuts at: 3237 3269 3574 3606 3691 3693 3723 3725 4969
Size: 32 305 32 85 2 30 2 1244
Cuts at: 4969 4999 5001 5031 1469
Size: 30 2 30 3567
Fragments arranged by size:
3567 1244 831 533 305 235 85 41
32 32 32 32 32 32 30 30
30 2 2 2
HincII GTy'rAC
Cuts at: 2541 4166 2541
Size: 1625 5504
HindIII A'AGCT_T
Cuts at: 575 575
Size: 7129
KasI G'GCGC_C
Cuts at: 4557 4805 4557
Size: 248 6881
KpnI G_GTAC'C
Cuts at: 1046 4147 1046
Size: 3101 4028
MluI A'CGCG_T
Cuts at: 6562 6562
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1005 2200 2614 2657 3223 3477 5800 5984 1005
Size: 1195 414 43 566 254 2323 184 2150
Fragments arranged by size:
2323 2150 1195 566 414 254 184 43
MscI TGG'CCA
Cuts at: 617 986 1342 1463 1903 2281 2549 4073 4888
Size: 369 356 121 440 378 268 1524 815
Cuts at: 4888 617
Size: 2858
Fragments arranged by size:
2858 1524 815 440 378 369 356 268
121
MslI CAynn'nnrTG
Cuts at: 724 2749 5116 5243 724
Size: 2025 2367 127 2610
Fragments arranged by size:
2610 2367 2025 127
MspA1I CmG'CkG
Cuts at: 214 429 1346 1417 1789 1871 1926 2121 2784
Size: 215 917 71 372 82 55 195 663
Cuts at: 2784 2998 3138 3979 4238 4552 4912 5927 6172
Size: 214 140 841 259 314 360 1015 245
Cuts at: 6172 214
Size: 1171
Fragments arranged by size:
1171 1015 917 841 663 372 360 314
259 245 215 214 195 140 82 71
55
NaeI GCC'GGC
Cuts at: 5308 5308
Size: 7129
NarI GG'CG_CC
Cuts at: 4558 4806 4558
Size: 248 6881
NcoI C'CATG_G
Cuts at: 125 125
Size: 7129
NgoMIV G'CCGG_C
Cuts at: 5306 5306
Size: 7129
NruI TCG'CGA
Cuts at: 5490 5490
Size: 7129
NspI r_CATG'y
Cuts at: 1818 4180 4680 5589 1818
Size: 2362 500 909 3358
Fragments arranged by size:
3358 2362 909 500
PasI CC'CwG_GG
Cuts at: 2293 2293
Size: 7129
PciI A'CATG_T
Cuts at: 5585 5585
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 3047 3964 3047
Size: 917 6212
PfoI T'CCnGG_A
Cuts at: 818 2344 2477 2498 2564 2792 2809 818
Size: 1526 133 21 66 228 17 5138
Fragments arranged by size:
5138 1526 228 133 66 21 17
PmeI GTTT'AAAC
Cuts at: 4126 4126
Size: 7129
PmlI CAC'GTG
Cuts at: 2699 2699
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6268 6300 6268
Size: 32 7097
PpuMI rG'GwC_Cy
Cuts at: 606 1410 2951 606
Size: 804 1541 4784
Fragments arranged by size:
4784 1541 804
PshAI GACnn'nnGTC
Cuts at: 847 847
Size: 7129
PspOMI G'GGCC_C
Cuts at: 207 2880 4044 207
Size: 2673 1164 3292
Fragments arranged by size:
3292 2673 1164
PstI C_TGCA'G
Cuts at: 2099 2783 3845 4174 4859 2099
Size: 684 1062 329 685 4369
Fragments arranged by size:
4369 1062 685 684 329
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7129
PvuII CAG'CTG
Cuts at: 214 1346 1417 1789 1926 2121 4552 4912 214
Size: 1132 71 372 137 195 2431 360 2431
Fragments arranged by size:
2431 2431 1132 372 360 195 137 71
RsrII CG'GwC_CG
Cuts at: 5322 5322
Size: 7129
SacI G_AGCT'C
Cuts at: 337 1253 2876 3008 4141 337
Size: 916 1623 132 1133 3325
Fragments arranged by size:
3325 1623 1133 916 132
SacII CC_GC'GG
Cuts at: 430 430
Size: 7129
SalI G'TCGA_C
Cuts at: 4164 4164
Size: 7129
SapI GCTCTTCn'nnn_
Cuts at: 1023 2084 5150 5360 1023
Size: 1061 3066 210 2792
Fragments arranged by size:
3066 2792 1061 210
SbfI CC_TGCA'GG
Cuts at: 4174 4174
Size: 7129
ScaI AGT'ACT
Cuts at: 3109 3649 6646 3109
Size: 540 2997 3592
Fragments arranged by size:
3592 2997 540
SexAI A'CCwGG_T
Cuts at: 3800 3800
Size: 7129
SfcI C'TryA_G
Cuts at: 33 1290 2095 2779 3681 3841 4170 4855 5850
Size: 1257 805 684 902 160 329 685 995
Cuts at: 5850 6041 33
Size: 191 1121
Fragments arranged by size:
1257 1121 995 902 805 685 684 329
191 160
SfoI GGC'GCC
Cuts at: 4559 4807 4559
Size: 248 6881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1102 1102
Size: 7129
SmaI CCC'GGG
Cuts at: 4092 4149 4092
Size: 57 7072
SmlI C'TyrA_G
Cuts at: 1006 2353 2602 4282 5197 5691 5953 1006
Size: 1347 249 1680 915 494 262 2182
Fragments arranged by size:
2182 1680 1347 915 494 262 249
SnaBI TAC'GTA
Cuts at: 4119 4119
Size: 7129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7129
SphI G_CATG'C
Cuts at: 4180 4680 4180
Size: 500 6629
SspI AAT'ATT
Cuts at: 369 369
Size: 7129
StuI AGG'CCT
Cuts at: 2030 2030
Size: 7129
StyI C'CwwG_G
Cuts at: 125 1388 1435 1643 1664 1916 2276 2441 2544
Size: 1263 47 208 21 252 360 165 103
Cuts at: 2544 2550 2719 3065 3345 3869 4074 4261 4615
Size: 6 169 346 280 524 205 187 354
Cuts at: 4615 125
Size: 2639
Fragments arranged by size:
2639 1263 524 360 354 346 280 252
208 205 187 169 165 103 47 21
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1134 1361 2099 3001 4710 4843 5302 5513 1134
Size: 227 738 902 1709 133 459 211 2750
Fragments arranged by size:
2750 1709 902 738 459 227 211 133
TatI w'GTAC_w
Cuts at: 373 3107 3119 3647 3725 6644 373
Size: 2734 12 528 78 2919 858
Fragments arranged by size:
2919 2734 858 528 78 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2653 2685 3925 3957 3996 4028 6646 6678 2653
Size: 32 1240 32 39 32 2618 32 3104
Fragments arranged by size:
3104 2618 1240 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1597 4924 1597
Size: 3327 3802
XbaI T'CTAG_A
Cuts at: 2052 4158 2052
Size: 2106 5023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 531 610 767 1923 3135 4622 531
Size: 79 157 1156 1212 1487 3038
Fragments arranged by size:
3038 1487 1212 1156 157 79
XmaI C'CCGG_G
Cuts at: 4090 4147 4090
Size: 57 7072
XmnI GAAnn'nnTTC
Cuts at: 2171 3751 2171
Size: 1580 5549
ZraI GAC'GTC
Cuts at: 1356 1356
Size: 7129
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BssHII ClaI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NsiI PacI PsiI PspXI
PsrI SanDI SfiI SrfI SwaI XhoI