Restriction map for
pF1KSDA0137
(Circular) (Six-base) MAPSORT of: pF1KSDA0137.seq Check: 7894 from: 1 to: 5435
pF1KSDA0137 5435 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2473 2473
Size: 5435
Acc65I G'GTAC_C
Cuts at: 2451 2451
Size: 5435
AclI AA'CG_TT
Cuts at: 1392 5275 1392
Size: 3883 1552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 2339 3260 3692 4439 4707 1795
Size: 544 921 432 747 268 2523
Fragments arranged by size:
2523 921 747 544 432 268
AflII C'TTAA_G
Cuts at: 1885 1885
Size: 5435
AflIII A'CryG_T
Cuts at: 2787 3891 4868 2787
Size: 1104 977 3354
Fragments arranged by size:
3354 1104 977
AgeI A'CCGG_T
Cuts at: 2226 3823 2226
Size: 1597 3838
AleI CACnn'nnGTG
Cuts at: 1421 1421
Size: 5435
AlwNI CAG_nnn'CTG
Cuts at: 819 2740 4307 819
Size: 1921 1567 1947
Fragments arranged by size:
1947 1921 1567
ApaLI G'TGCA_C
Cuts at: 2299 4205 2299
Size: 1906 3529
ApoI r'AATT_y
Cuts at: 642 1281 1328 2349 2439 4788 5044 642
Size: 639 47 1021 90 2349 256 1033
Fragments arranged by size:
2349 1033 1021 639 256 90 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 5435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
AvaI C'yCGr_G
Cuts at: 198 1024 2455 198
Size: 826 1431 3178
Fragments arranged by size:
3178 1431 826
BamHI G'GATC_C
Cuts at: 291 2460 291
Size: 2169 3266
BanI G'GyrC_C
Cuts at: 1200 2451 2865 3113 3148 1200
Size: 1251 414 248 35 3487
Fragments arranged by size:
3487 1251 414 248 35
BanII G_rGCy'C
Cuts at: 205 2449 3479 205
Size: 2244 1030 2161
Fragments arranged by size:
2244 2161 1030
BbeI G_GCGC'C
Cuts at: 2869 3117 2869
Size: 248 5187
BciVI GTATCCnnnnn_n'
Cuts at: 1707 3333 4094 5435 1707
Size: 1626 761 1341 1707
Fragments arranged by size:
1707 1626 1341 761
BclI T'GATC_A
Cuts at: 2955 2955
Size: 5435
BglII A'GATC_T
Cuts at: 2950 4987 2950
Size: 2037 3398
BlpI GC'TnA_GC
Cuts at: 2547 2547
Size: 5435
Bme1580I G_kGCm'C
Cuts at: 1031 2303 3060 3153 4209 1031
Size: 1272 757 93 1056 2257
Fragments arranged by size:
2257 1272 1056 757 93
BmgBI CAC'GTC
Cuts at: 188 550 188
Size: 362 5073
BmrI ACTGGGnnnn_n'
Cuts at: 2647 2728 3063 5207 2647
Size: 81 335 2144 2875
Fragments arranged by size:
2875 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3802 3802
Size: 5435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 1623 350
Size: 1273 4162
Bpu10I CC'TnA_GC
Cuts at: 853 853
Size: 5435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 2611 3490 3982 4280 582
Size: 2029 879 492 298 1737
Fragments arranged by size:
2029 1737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5139 5139
Size: 5435
BsaAI yAC'GTr
Cuts at: 1423 2427 2788 1423
Size: 1004 361 4070
Fragments arranged by size:
4070 1004 361
BsaBI GATnn'nnATC
Cuts at: 6 1769 2949 4986 6
Size: 1763 1180 2037 455
Fragments arranged by size:
2037 1763 1180 455
BsaHI Gr'CG_yC
Cuts at: 2866 3114 3816 5312 2866
Size: 248 702 1496 2989
Fragments arranged by size:
2989 1496 702 248
BsaWI w'CCGG_w
Cuts at: 459 2226 2624 3145 3823 4097 4244 4655 459
Size: 1767 398 521 678 274 147 411 1239
Fragments arranged by size:
1767 1239 678 521 411 398 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 626 626
Size: 5435
BseYI C'CCAG_C
Cuts at: 176 2374 2860 3618 4195 4691 176
Size: 2198 486 758 577 496 920
Fragments arranged by size:
2198 920 758 577 496 486
BsiEI CG_ry'CG
Cuts at: 104 682 2977 3023 4231 104
Size: 578 2295 46 1208 1308
Fragments arranged by size:
2295 1308 1208 578 46
BsiHKAI G_wGCw'C
Cuts at: 349 2303 2449 3227 3417 4209 349
Size: 1954 146 778 190 792 1575
Fragments arranged by size:
1954 1575 792 778 190 146
BsmBI CGTCTCn'nnnn_
Cuts at: 194 194
Size: 5435
Bsp1286I G_dGCh'C
Cuts at: 205 349 1031 2303 2449 3060 3153 3227 3417
Size: 144 682 1272 146 611 93 74 190
Cuts at: 3417 3479 4209 205
Size: 62 730 1431
Fragments arranged by size:
1431 1272 730 682 611 190 146 144
93 74 62
BspEI T'CCGG_A
Cuts at: 4655 4655
Size: 5435
BspHI T'CATG_A
Cuts at: 1314 4611 1314
Size: 3297 2138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2485 2699 3001 3382 2485
Size: 214 302 381 4538
Fragments arranged by size:
4538 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3727 3781 5265 5430 50
Size: 3677 54 1484 165 55
Fragments arranged by size:
3677 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 275 1761 3347 275
Size: 1486 1586 2363
Fragments arranged by size:
2363 1586 1486
BsrFI r'CCGG_y
Cuts at: 2226 3433 3614 3823 2226
Size: 1207 181 209 3838
Fragments arranged by size:
3838 1207 209 181
BsrGI T'GTAC_A
Cuts at: 729 1729 729
Size: 1000 4435
BssSI C'ACGA_G
Cuts at: 3706 4064 3706
Size: 358 5077
BstAPI GCAn_nnn'nTGC
Cuts at: 2812 2812
Size: 5435
BstBI TT'CG_AA
Cuts at: 110 3796 110
Size: 3686 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1965 1965
Size: 5435
BstYI r'GATC_y
Cuts at: 1 291 430 1998 2460 2927 2950 3285 3531
Size: 290 139 1568 462 467 23 335 246
Cuts at: 3531 4543 4629 4641 4987 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1568 1012 467 462 449 346 335 290
246 139 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3385 3566 3729 3385
Size: 181 163 5091
Fragments arranged by size:
5091 181 163
BtsI GCAGTG_nn'
Cuts at: 150 375 2244 2678 150
Size: 225 1869 434 2907
Fragments arranged by size:
2907 1869 434 225
ClaI AT'CG_AT
Cuts at: 1014 2289 1014
Size: 1275 4160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1277 1312 1277
Size: 35 5400
DraI TTT'AAA
Cuts at: 753 2434 753
Size: 1681 3754
DrdI GACnn_nn'nnGTC
Cuts at: 3141 3999 3141
Size: 858 4577
EaeI y'GGCC_r
Cuts at: 215 552 3020 3194 3585 3612 4957 215
Size: 337 2468 174 391 27 1345 693
Fragments arranged by size:
2468 1345 693 391 337 174 27
EagI C'GGCC_G
Cuts at: 3020 3020
Size: 5435
EarI CTCTTCn'nnn_
Cuts at: 994 1358 3458 3668 994
Size: 364 2100 210 2761
Fragments arranged by size:
2761 2100 364 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3643 3953 4099 5245 3643
Size: 310 146 1146 3833
Fragments arranged by size:
3833 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2447 2447
Size: 5435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 1623 1795 2339 3260 3692 4439 4707 350
Size: 1273 172 544 921 432 747 268 1078
Fragments arranged by size:
1273 1078 921 747 544 432 268 172
EcoO109I rG'GnC_Cy
Cuts at: 221 816 2574 221
Size: 595 1758 3082
Fragments arranged by size:
3082 1758 595
EcoRI G'AATT_C
Cuts at: 2439 2439
Size: 5435
EcoRV GAT'ATC
Cuts at: 1846 1846
Size: 5435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 3454 2191
Size: 1263 4172
FspI TGC'GCA
Cuts at: 3216 3216
Size: 5435
HaeII r_GCGC'y
Cuts at: 1055 2869 3117 4139 5118 1055
Size: 1814 248 1022 979 1372
Fragments arranged by size:
1814 1372 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 1007 1039 1690 1722 2083 2115 3277
Size: 32 542 32 651 32 361 32 1162
Cuts at: 3277 3307 3309 3339 433
Size: 30 2 30 2529
Fragments arranged by size:
2529 1162 651 542 361 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1298 2474 1298
Size: 1176 4259
HindIII A'AGCT_T
Cuts at: 423 423
Size: 5435
KasI G'GCGC_C
Cuts at: 2865 3113 2865
Size: 248 5187
KpnI G_GTAC'C
Cuts at: 2455 2455
Size: 5435
MluI A'CGCG_T
Cuts at: 4868 4868
Size: 5435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 217 354 2037 2085 2105 4106 4290 217
Size: 137 1683 48 20 2001 184 1362
Fragments arranged by size:
2001 1683 1362 184 137 48 20
MscI TGG'CCA
Cuts at: 554 3196 554
Size: 2642 2793
MslI CAynn'nnrTG
Cuts at: 940 1421 1634 3424 3551 940
Size: 481 213 1790 127 2824
Fragments arranged by size:
2824 1790 481 213 127
MspA1I CmG'CkG
Cuts at: 2307 2546 2860 3220 4233 4478 2307
Size: 239 314 360 1013 245 3264
Fragments arranged by size:
3264 1013 360 314 245 239
NaeI GCC'GGC
Cuts at: 3616 3616
Size: 5435
NarI GG'CG_CC
Cuts at: 2866 3114 2866
Size: 248 5187
NgoMIV G'CCGG_C
Cuts at: 3614 3614
Size: 5435
NspI r_CATG'y
Cuts at: 1639 1925 2488 2988 3895 1639
Size: 286 563 500 907 3179
Fragments arranged by size:
3179 907 563 500 286
PasI CC'CwG_GG
Cuts at: 257 2010 257
Size: 1753 3682
PciI A'CATG_T
Cuts at: 3891 3891
Size: 5435
PflMI CCAn_nnn'nTGG
Cuts at: 819 819
Size: 5435
PfoI T'CCnGG_A
Cuts at: 247 812 247
Size: 565 4870
PmeI GTTT'AAAC
Cuts at: 2434 2434
Size: 5435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 4574
Size: 32 5403
PpuMI rG'GwC_Cy
Cuts at: 221 816 221
Size: 595 4840
PsiI TTA'TAA
Cuts at: 2257 2257
Size: 5435
PstI C_TGCA'G
Cuts at: 1645 2482 3167 1645
Size: 837 685 3913
Fragments arranged by size:
3913 837 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5435
PvuII CAG'CTG
Cuts at: 2307 2860 3220 2307
Size: 553 360 4522
Fragments arranged by size:
4522 553 360
RsrII CG'GwC_CG
Cuts at: 3630 3630
Size: 5435
SacI G_AGCT'C
Cuts at: 2449 2449
Size: 5435
SalI G'TCGA_C
Cuts at: 2472 2472
Size: 5435
SapI GCTCTTCn'nnn_
Cuts at: 3458 3668 3458
Size: 210 5225
SbfI CC_TGCA'GG
Cuts at: 2482 2482
Size: 5435
ScaI AGT'ACT
Cuts at: 4952 4952
Size: 5435
SfcI C'TryA_G
Cuts at: 33 1641 2478 3163 4156 4347 33
Size: 1608 837 685 993 191 1121
Fragments arranged by size:
1608 1121 993 837 685 191
SfoI GGC'GCC
Cuts at: 2867 3115 2867
Size: 248 5187
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
SmaI CCC'GGG
Cuts at: 200 2457 200
Size: 2257 3178
SmlI C'TyrA_G
Cuts at: 597 1885 2590 3505 3997 4259 597
Size: 1288 705 915 492 262 1773
Fragments arranged by size:
1773 1288 915 705 492 262
SnaBI TAC'GTA
Cuts at: 2427 2427
Size: 5435
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5435
SphI G_CATG'C
Cuts at: 2488 2988 2488
Size: 500 4935
SspI AAT'ATT
Cuts at: 2192 2192
Size: 5435
StyI C'CwwG_G
Cuts at: 1709 2246 2569 2923 1709
Size: 537 323 354 4221
Fragments arranged by size:
4221 537 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 696 1107 1487 1799 3018 3151 3610 3819 696
Size: 411 380 312 1219 133 459 209 2312
Fragments arranged by size:
2312 1219 459 411 380 312 209 133
TatI w'GTAC_w
Cuts at: 729 970 1729 4950 729
Size: 241 759 3221 1214
Fragments arranged by size:
3221 1214 759 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4952 4984 4952
Size: 32 5403
Tth111I GACn'n_nGTC
Cuts at: 3232 3232
Size: 5435
XbaI T'CTAG_A
Cuts at: 2466 2466
Size: 5435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 142 589 2930 142
Size: 447 2341 2647
Fragments arranged by size:
2647 2341 447
XmaI C'CCGG_G
Cuts at: 198 2455 198
Size: 2257 3178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsgI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I
BtgI DraIII EcoNI FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI ZraI