Restriction map for
pF1KSDA0139
(Circular) (Six-base) MAPSORT of: pF1KSDA0139.seq Check: 6460 from: 1 to: 7283
pF1KSDA0139 7283 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1440 1440
Size: 7283
AccI GT'mk_AC
Cuts at: 1081 4321 1081
Size: 3240 4043
Acc65I G'GTAC_C
Cuts at: 4299 4299
Size: 7283
AclI AA'CG_TT
Cuts at: 2232 7123 2232
Size: 4891 2392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1655 2855 3825 5108 5540 6287 6555 449
Size: 1206 1200 970 1283 432 747 268 1177
Fragments arranged by size:
1283 1206 1200 1177 970 747 432 268
AfeI AGC'GCT
Cuts at: 2262 2262
Size: 7283
AflIII A'CryG_T
Cuts at: 691 4635 5739 6716 691
Size: 3944 1104 977 1258
Fragments arranged by size:
3944 1258 1104 977
AgeI A'CCGG_T
Cuts at: 5671 5671
Size: 7283
AhdI GACnn_n'nnGTC
Cuts at: 3640 3640
Size: 7283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3037 3069 3037
Size: 32 7251
AlwNI CAG_nnn'CTG
Cuts at: 2845 4588 6155 2845
Size: 1743 1567 3973
Fragments arranged by size:
3973 1743 1567
ApaI G_GGCC'C
Cuts at: 3541 3541
Size: 7283
ApaLI G'TGCA_C
Cuts at: 6053 6053
Size: 7283
ApoI r'AATT_y
Cuts at: 175 577 673 703 1315 1803 2243 3506 4287
Size: 402 96 30 612 488 440 1263 781
Cuts at: 4287 6636 6892 175
Size: 2349 256 566
Fragments arranged by size:
2349 1263 781 612 566 488 440 402
256 96 30
AseI AT'TA_AT
Cuts at: 20 1238 20
Size: 1218 6065
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7283
AvaI C'yCGr_G
Cuts at: 1628 3701 4303 1628
Size: 2073 602 4608
Fragments arranged by size:
4608 2073 602
AvrII C'CTAG_G
Cuts at: 3850 3850
Size: 7283
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2638 2671 2638
Size: 33 7250
BamHI G'GATC_C
Cuts at: 1826 2118 4308 1826
Size: 292 2190 4801
Fragments arranged by size:
4801 2190 292
BanI G'GyrC_C
Cuts at: 2071 2815 4299 4713 4961 4996 2071
Size: 744 1484 414 248 35 4358
Fragments arranged by size:
4358 1484 744 414 248 35
BanII G_rGCy'C
Cuts at: 2969 3541 4297 5327 2969
Size: 572 756 1030 4925
Fragments arranged by size:
4925 1030 756 572
BbeI G_GCGC'C
Cuts at: 4717 4965 4717
Size: 248 7035
BbsI GAAGACnn'nnnn_
Cuts at: 536 1198 2108 2396 2760 3143 3852 536
Size: 662 910 288 364 383 709 3967
Fragments arranged by size:
3967 910 709 662 383 364 288
BbvCI CC'TCA_GC
Cuts at: 1946 3884 1946
Size: 1938 5345
BciVI GTATCCnnnnn_n'
Cuts at: 5181 5942 7283 5181
Size: 761 1341 5181
Fragments arranged by size:
5181 1341 761
BclI T'GATC_A
Cuts at: 1578 4803 1578
Size: 3225 4058
BfrBI ATG'CAT
Cuts at: 810 1003 810
Size: 193 7090
BglI GCCn_nnn'nGGC
Cuts at: 3090 3090
Size: 7283
BglII A'GATC_T
Cuts at: 3987 4798 6835 3987
Size: 811 2037 4435
Fragments arranged by size:
4435 2037 811
BlpI GC'TnA_GC
Cuts at: 4395 4395
Size: 7283
Bme1580I G_kGCm'C
Cuts at: 3541 4908 5001 6057 3541
Size: 1367 93 1056 4767
Fragments arranged by size:
4767 1367 1056 93
BmgBI CAC'GTC
Cuts at: 2884 3034 3244 3577 4060 2884
Size: 150 210 333 483 6107
Fragments arranged by size:
6107 483 333 210 150
BmrI ACTGGGnnnn_n'
Cuts at: 2077 4495 4576 4911 7055 2077
Size: 2418 81 335 2144 2305
Fragments arranged by size:
2418 2305 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4203 5650 4203
Size: 1447 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1912 2176 2841 3888 3921 403
Size: 1509 264 665 1047 33 3765
Fragments arranged by size:
3765 1509 1047 665 264 33
Bpu10I CC'TnA_GC
Cuts at: 1124 1946 3884 1124
Size: 822 1938 4523
Fragments arranged by size:
4523 1938 822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1891 2128 3600 3894 4459 5338 5830 6128
Size: 1689 237 1472 294 565 879 492 298
Cuts at: 6128 202
Size: 1357
Fragments arranged by size:
1689 1472 1357 879 565 492 298 294
237
BsaI GGTCTCn'nnnn_
Cuts at: 2915 3627 4130 4142 4148 6987 2915
Size: 712 503 12 6 2839 3211
Fragments arranged by size:
3211 2839 712 503 12 6
BsaAI yAC'GTr
Cuts at: 4275 4636 4275
Size: 361 6922
BsaBI GATnn'nnATC
Cuts at: 6 3350 3470 3560 3587 3824 4797 6834 6
Size: 3344 120 90 27 237 973 2037 455
Fragments arranged by size:
3344 2037 973 455 237 120 90 27
BsaHI Gr'CG_yC
Cuts at: 2940 3153 4714 4962 5664 7160 2940
Size: 213 1561 248 702 1496 3063
Fragments arranged by size:
3063 1561 1496 702 248 213
BsaWI w'CCGG_w
Cuts at: 1389 4472 4993 5671 5945 6092 6503 1389
Size: 3083 521 678 274 147 411 2169
Fragments arranged by size:
3083 2169 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1867 2575 2578 3290 3697 3790 4094 1867
Size: 708 3 712 407 93 304 5056
Fragments arranged by size:
5056 712 708 407 304 93 3
BseYI C'CCAG_C
Cuts at: 659 3689 4122 4708 5466 6043 6539 659
Size: 3030 433 586 758 577 496 1403
Fragments arranged by size:
3030 1403 758 586 577 496 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 3542 2408
Size: 1134 6149
BsiEI CG_ry'CG
Cuts at: 104 4825 4871 6079 104
Size: 4721 46 1208 1308
Fragments arranged by size:
4721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1820 4297 5075 5265 6057 1820
Size: 2477 778 190 792 3046
Fragments arranged by size:
3046 2477 792 778 190
BsiWI C'GTAC_G
Cuts at: 1129 1129
Size: 7283
BsmI GAATG_Cn'
Cuts at: 737 871 2080 2387 737
Size: 134 1209 307 5633
Fragments arranged by size:
5633 1209 307 134
BsmBI CGTCTCn'nnnn_
Cuts at: 2874 3989 4050 2874
Size: 1115 61 6107
Fragments arranged by size:
6107 1115 61
Bsp1286I G_dGCh'C
Cuts at: 1820 2969 3541 4297 4908 5001 5075 5265 5327
Size: 1149 572 756 611 93 74 190 62
Cuts at: 5327 6057 1820
Size: 730 3046
Fragments arranged by size:
3046 1149 756 730 611 572 190 93
74 62
BspEI T'CCGG_A
Cuts at: 6503 6503
Size: 7283
BspHI T'CATG_A
Cuts at: 6459 6459
Size: 7283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1440 1557 4333 4547 4849 5230 1440
Size: 117 2776 214 302 381 3493
Fragments arranged by size:
3493 2776 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 2601 2664 2931 4095 5575 5629 7113 7278
Size: 2551 63 267 1164 1480 54 1484 165
Cuts at: 7278 50
Size: 55
Fragments arranged by size:
2551 1484 1480 1164 267 165 63 55
54
BsrDI GCAATG_nn'
Cuts at: 1554 5195 1554
Size: 3641 3642
BsrFI r'CCGG_y
Cuts at: 129 3080 3962 5281 5462 5671 129
Size: 2951 882 1319 181 209 1741
Fragments arranged by size:
2951 1741 1319 882 209 181
BssHII G'CGCG_C
Cuts at: 2616 2616
Size: 7283
BssSI C'ACGA_G
Cuts at: 5554 5912 5554
Size: 358 6925
BstAPI GCAn_nnn'nTGC
Cuts at: 1407 1665 4660 1407
Size: 258 2995 4030
Fragments arranged by size:
4030 2995 258
BstBI TT'CG_AA
Cuts at: 110 5644 110
Size: 5534 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 813 2235 3656 813
Size: 1422 1421 4440
Fragments arranged by size:
4440 1422 1421
BstYI r'GATC_y
Cuts at: 1 303 474 1606 1826 2118 2292 3987 4308
Size: 302 171 1132 220 292 174 1695 321
Cuts at: 4308 4775 4798 5133 5379 6391 6477 6489 6835
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6835 1
Size: 449
Fragments arranged by size:
1695 1132 1012 467 449 346 335 321
302 292 246 220 174 171 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 3041 3041
Size: 7283
BtgI C'CryG_G
Cuts at: 568 568
Size: 7283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5233 5414 5577 5233
Size: 181 163 6939
Fragments arranged by size:
6939 181 163
BtsI GCAGTG_nn'
Cuts at: 607 1787 4526 607
Size: 1180 2739 3364
Fragments arranged by size:
3364 2739 1180
ClaI AT'CG_AT
Cuts at: 2493 2493
Size: 7283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3633 3668 3633
Size: 35 7248
DraI TTT'AAA
Cuts at: 4282 4282
Size: 7283
DrdI GACnn_nn'nnGTC
Cuts at: 1701 4989 5847 1701
Size: 3288 858 3137
Fragments arranged by size:
3288 3137 858
EaeI y'GGCC_r
Cuts at: 4868 5042 5433 5460 6805 4868
Size: 174 391 27 1345 5346
Fragments arranged by size:
5346 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4868 4868
Size: 7283
EarI CTCTTCn'nnn_
Cuts at: 1946 2459 2552 2600 2636 2741 2850 2913 2921
Size: 513 93 48 36 105 109 63 8
Cuts at: 2921 3867 4005 4085 5306 5516 1946
Size: 946 138 80 1221 210 3713
Fragments arranged by size:
3713 1221 946 513 210 138 109 105
93 80 63 48 36 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1426 5491 5801 5947 7093 1426
Size: 4065 310 146 1146 1616
Fragments arranged by size:
4065 1616 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4295 4295
Size: 7283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 449 1655 1912 2176 2841 2855 3825 3888
Size: 46 1206 257 264 665 14 970 63
Cuts at: 3888 3921 5108 5540 6287 6555 403
Size: 33 1187 432 747 268 1131
Fragments arranged by size:
1206 1187 1131 970 747 665 432 268
264 257 63 46 33 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3182 3182
Size: 7283
EcoO109I rG'GnC_Cy
Cuts at: 2830 3025 3328 3358 3388 3418 3448 3478 3537
Size: 195 303 30 30 30 30 30 59
Cuts at: 3537 3538 3789 4015 4098 4422 2830
Size: 1 251 226 83 324 5691
Fragments arranged by size:
5691 324 303 251 226 195 83 59
30 30 30 30 30 1
EcoRI G'AATT_C
Cuts at: 703 3506 4287 703
Size: 2803 781 3699
Fragments arranged by size:
3699 2803 781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 1685 1885 5302 336
Size: 1349 200 3417 2317
Fragments arranged by size:
3417 2317 1349 200
FspI TGC'GCA
Cuts at: 5064 5064
Size: 7283
HaeII r_GCGC'y
Cuts at: 642 756 1869 2264 3284 4717 4965 5987 6966
Size: 114 1113 395 1020 1433 248 1022 979
Cuts at: 6966 642
Size: 959
Fragments arranged by size:
1433 1113 1022 1020 979 959 395 248
114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 1035 1067 1635 1667 1818 1850 2893
Size: 32 216 32 568 32 151 32 1043
Cuts at: 2893 2925 3040 3072 3280 3283 3312 3315 3626
Size: 32 115 32 208 3 29 3 311
Cuts at: 3626 3658 3678 3710 4007 4039 4198 4230 5125
Size: 32 20 32 297 32 159 32 895
Cuts at: 5125 5155 5157 5187 787
Size: 30 2 30 2883
Fragments arranged by size:
2883 1043 895 568 311 297 216 208
159 151 115 32 32 32 32 32
32 32 32 32 32 30 30 29
20 3 3 2
HincII GTy'rAC
Cuts at: 361 4322 361
Size: 3961 3322
HindIII A'AGCT_T
Cuts at: 665 665
Size: 7283
KasI G'GCGC_C
Cuts at: 4713 4961 4713
Size: 248 7035
KpnI G_GTAC'C
Cuts at: 4303 4303
Size: 7283
MfeI C'AATT_G
Cuts at: 1299 1847 1299
Size: 548 6735
MluI A'CGCG_T
Cuts at: 691 6716 691
Size: 6025 1258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1545 2693 3323 3443 5954 6138 1545
Size: 1148 630 120 2511 184 2690
Fragments arranged by size:
2690 2511 1148 630 184 120
MscI TGG'CCA
Cuts at: 5044 5044
Size: 7283
MslI CAynn'nnrTG
Cuts at: 3161 5272 5399 3161
Size: 2111 127 5045
Fragments arranged by size:
5045 2111 127
MspA1I CmG'CkG
Cuts at: 834 1692 1824 3220 3883 4394 4708 5068 6081
Size: 858 132 1396 663 511 314 360 1013
Cuts at: 6081 6326 834
Size: 245 1791
Fragments arranged by size:
1791 1396 1013 858 663 511 360 314
245 132
NaeI GCC'GGC
Cuts at: 131 5464 131
Size: 5333 1950
NarI GG'CG_CC
Cuts at: 4714 4962 4714
Size: 248 7035
NcoI C'CATG_G
Cuts at: 568 568
Size: 7283
NgoMIV G'CCGG_C
Cuts at: 129 5462 129
Size: 5333 1950
NsiI A_TGCA'T
Cuts at: 812 1005 812
Size: 193 7090
NspI r_CATG'y
Cuts at: 1668 4336 4836 5743 1668
Size: 2668 500 907 3208
Fragments arranged by size:
3208 2668 907 500
PciI A'CATG_T
Cuts at: 5739 5739
Size: 7283
PflMI CCAn_nnn'nTGG
Cuts at: 3244 3244
Size: 7283
PmeI GTTT'AAAC
Cuts at: 4282 4282
Size: 7283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6422 6454 6422
Size: 32 7251
PpuMI rG'GwC_Cy
Cuts at: 2830 3328 3358 3388 3418 3448 3478 3789 4015
Size: 498 30 30 30 30 30 311 226
Cuts at: 4015 4098 2830
Size: 83 6015
Fragments arranged by size:
6015 498 311 226 83 30 30 30
30 30
PshAI GACnn'nnGTC
Cuts at: 3326 3386 3416 3446 3911 3326
Size: 60 30 30 465 6698
Fragments arranged by size:
6698 465 60 30 30
PspOMI G'GGCC_C
Cuts at: 3537 3537
Size: 7283
PstI C_TGCA'G
Cuts at: 1448 4330 5015 1448
Size: 2882 685 3716
Fragments arranged by size:
3716 2882 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7283
PvuII CAG'CTG
Cuts at: 834 1692 3220 3883 4708 5068 834
Size: 858 1528 663 825 360 3049
Fragments arranged by size:
3049 1528 858 825 663 360
RsrII CG'GwC_CG
Cuts at: 5478 5478
Size: 7283
SacI G_AGCT'C
Cuts at: 4297 4297
Size: 7283
SalI G'TCGA_C
Cuts at: 4320 4320
Size: 7283
SanDI GG'GwC_CC
Cuts at: 3328 3358 3388 3418 3448 4098 3328
Size: 30 30 30 30 650 6513
Fragments arranged by size:
6513 650 30 30 30 30
SapI GCTCTTCn'nnn_
Cuts at: 2459 2636 2921 4085 5306 5516 2459
Size: 177 285 1164 1221 210 4226
Fragments arranged by size:
4226 1221 1164 285 210 177
SbfI CC_TGCA'GG
Cuts at: 4330 4330
Size: 7283
ScaI AGT'ACT
Cuts at: 1712 6800 1712
Size: 5088 2195
SexAI A'CCwGG_T
Cuts at: 3621 3621
Size: 7283
SfcI C'TryA_G
Cuts at: 33 1444 2544 4326 5011 6004 6195 33
Size: 1411 1100 1782 685 993 191 1121
Fragments arranged by size:
1782 1411 1121 1100 993 685 191
SfoI GGC'GCC
Cuts at: 4715 4963 4715
Size: 248 7035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7283
SmaI CCC'GGG
Cuts at: 4305 4305
Size: 7283
SmlI C'TyrA_G
Cuts at: 181 1628 1870 2107 3615 3701 3909 4438 5353
Size: 1447 242 237 1508 86 208 529 915
Cuts at: 5353 5845 6107 181
Size: 492 262 1357
Fragments arranged by size:
1508 1447 1357 915 529 492 262 242
237 208 86
SnaBI TAC'GTA
Cuts at: 4275 4275
Size: 7283
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7283
SphI G_CATG'C
Cuts at: 1668 4336 4836 1668
Size: 2668 500 4115
Fragments arranged by size:
4115 2668 500
SspI AAT'ATT
Cuts at: 486 1884 486
Size: 1398 5885
StuI AGG'CCT
Cuts at: 3114 4186 3114
Size: 1072 6211
StyI C'CwwG_G
Cuts at: 568 3481 3541 3628 3850 4417 4771 568
Size: 2913 60 87 222 567 354 3080
Fragments arranged by size:
3080 2913 567 354 222 87 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2059 4022 4154 4166 4172 4866 4999 5458 5667
Size: 1963 132 12 6 694 133 459 209
Cuts at: 5667 2059
Size: 3675
Fragments arranged by size:
3675 1963 694 459 209 133 132 12
6
TatI w'GTAC_w
Cuts at: 1260 1710 6798 1260
Size: 450 5088 1745
Fragments arranged by size:
5088 1745 450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6800 6832 6800
Size: 32 7251
Tth111I GACn'n_nGTC
Cuts at: 3035 3623 5080 3035
Size: 588 1457 5238
Fragments arranged by size:
5238 1457 588
XbaI T'CTAG_A
Cuts at: 477 628 1033 2776 4314 477
Size: 151 405 1743 1538 3446
Fragments arranged by size:
3446 1743 1538 405 151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2873 3339 3369 3399 3429 3459 4778 2873
Size: 466 30 30 30 30 1319 5378
Fragments arranged by size:
5378 1319 466 30 30 30 30
XhoI C'TCGA_G
Cuts at: 1628 3701 1628
Size: 2073 5210
XmaI C'CCGG_G
Cuts at: 4303 4303
Size: 7283
XmnI GAAnn'nnTTC
Cuts at: 624 890 2243 624
Size: 266 1353 5664
Fragments arranged by size:
5664 1353 266
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AscI BcgI BmtI BsaXI
BsrGI BstEII BstZ17I DraIII EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PsiI PspXI PsrI SacII SfiI SgrAI SrfI SwaI
ZraI