Restriction map for
pF1KSDA0141
(Circular) (Six-base) MAPSORT of: pF1KSDA0141.seq Check: 6606 from: 1 to: 4684
pF1KSDA0141 4684 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1720 1720
Size: 4684
Acc65I G'GTAC_C
Cuts at: 1289 1698 1289
Size: 409 4275
AclI AA'CG_TT
Cuts at: 4524 4524
Size: 4684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1489 2507 2939 3688 3956 1171
Size: 318 1018 432 749 268 1899
Fragments arranged by size:
1899 1018 749 432 318 268
AflIII A'CryG_T
Cuts at: 2034 3140 4117 2034
Size: 1106 977 2601
Fragments arranged by size:
2601 1106 977
AgeI A'CCGG_T
Cuts at: 3072 3072
Size: 4684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 986 986
Size: 4684
AlwNI CAG_nnn'CTG
Cuts at: 1987 3556 1987
Size: 1569 3115
ApaI G_GGCC'C
Cuts at: 1445 1445
Size: 4684
ApaLI G'TGCA_C
Cuts at: 3454 3454
Size: 4684
ApoI r'AATT_y
Cuts at: 1301 1686 4037 4293 1301
Size: 385 2351 256 1692
Fragments arranged by size:
2351 1692 385 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4684
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4684
AvaI C'yCGr_G
Cuts at: 138 1195 1437 1542 1702 138
Size: 1057 242 105 160 3120
Fragments arranged by size:
3120 1057 242 160 105
BamHI G'GATC_C
Cuts at: 1707 1707
Size: 4684
BanI G'GyrC_C
Cuts at: 131 524 1000 1088 1289 1698 2112 2360 2395
Size: 393 476 88 201 409 414 248 35
Cuts at: 2395 131
Size: 2420
Fragments arranged by size:
2420 476 414 409 393 248 201 88
35
BanII G_rGCy'C
Cuts at: 159 672 1220 1413 1445 1696 2726 159
Size: 513 548 193 32 251 1030 2117
Fragments arranged by size:
2117 1030 548 513 251 193 32
BbeI G_GCGC'C
Cuts at: 135 2116 2364 135
Size: 1981 248 2455
Fragments arranged by size:
2455 1981 248
BbsI GAAGACnn'nnnn_
Cuts at: 338 338
Size: 4684
BbvCI CC'TCA_GC
Cuts at: 461 627 764 461
Size: 166 137 4381
Fragments arranged by size:
4381 166 137
BciVI GTATCCnnnnn_n'
Cuts at: 2580 3343 4684 2580
Size: 763 1341 2580
Fragments arranged by size:
2580 1341 763
BclI T'GATC_A
Cuts at: 2202 2202
Size: 4684
BglI GCCn_nnn'nGGC
Cuts at: 406 569 406
Size: 163 4521
BglII A'GATC_T
Cuts at: 2197 4236 2197
Size: 2039 2645
BlpI GC'TnA_GC
Cuts at: 632 1794 632
Size: 1162 3522
Bme1580I G_kGCm'C
Cuts at: 394 1445 2307 2400 3458 394
Size: 1051 862 93 1058 1620
Fragments arranged by size:
1620 1058 1051 862 93
BmrI ACTGGGnnnn_n'
Cuts at: 181 1894 1975 2310 4456 181
Size: 1713 81 335 2146 409
Fragments arranged by size:
2146 1713 409 335 81
BmtI G_CTAG'C
Cuts at: 1516 1516
Size: 4684
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 212 686 3049 212
Size: 474 2363 1847
Fragments arranged by size:
2363 1847 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 1464 1590 207
Size: 1257 126 3301
Fragments arranged by size:
3301 1257 126
Bpu10I CC'TnA_GC
Cuts at: 461 627 764 1124 461
Size: 166 137 360 4021
Fragments arranged by size:
4021 360 166 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1194 1371 1858 2737 3231 3529 832
Size: 362 177 487 879 494 298 1987
Fragments arranged by size:
1987 879 494 487 362 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 1094 4388 1094
Size: 3294 1390
BsaAI yAC'GTr
Cuts at: 1674 2035 1674
Size: 361 4323
BsaBI GATnn'nnATC
Cuts at: 6 2196 4235 6
Size: 2190 2039 455
Fragments arranged by size:
2190 2039 455
BsaHI Gr'CG_yC
Cuts at: 132 2113 2361 3065 4561 132
Size: 1981 248 704 1496 255
Fragments arranged by size:
1981 1496 704 255 248
BsaWI w'CCGG_w
Cuts at: 1871 2392 3072 3346 3493 3904 1871
Size: 521 680 274 147 411 2651
Fragments arranged by size:
2651 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 539 547 569 517
Size: 22 8 22 4632
Fragments arranged by size:
4632 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 225 525 680 829 1555 225
Size: 300 155 149 726 3354
Fragments arranged by size:
3354 726 300 155 149
BseYI C'CCAG_C
Cuts at: 559 573 758 1104 1444 2107 2865 3444 3940
Size: 14 185 346 340 663 758 579 496
Cuts at: 3940 559
Size: 1303
Fragments arranged by size:
1303 758 663 579 496 346 340 185
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1348 1348
Size: 4684
BsiEI CG_ry'CG
Cuts at: 104 2224 2270 3480 104
Size: 2120 46 1210 1308
Fragments arranged by size:
2120 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 159 672 1549 1696 2474 2664 3458 159
Size: 513 877 147 778 190 794 1385
Fragments arranged by size:
1385 877 794 778 513 190 147
Bsp1286I G_dGCh'C
Cuts at: 159 394 672 1220 1413 1445 1549 1696 2307
Size: 235 278 548 193 32 104 147 611
Cuts at: 2307 2400 2474 2664 2726 3458 159
Size: 93 74 190 62 732 1385
Fragments arranged by size:
1385 732 611 548 278 235 193 190
147 104 93 74 62 32
BspEI T'CCGG_A
Cuts at: 3904 3904
Size: 4684
BspHI T'CATG_A
Cuts at: 984 3860 984
Size: 2876 1808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1277 1732 1946 2248 2629 1277
Size: 455 214 302 381 3332
Fragments arranged by size:
3332 455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 299 1128 1375 2974 3028 4514 4679 50
Size: 249 829 247 1599 54 1486 165 55
Fragments arranged by size:
1599 1486 829 249 247 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2594 2594
Size: 4684
BsrFI r'CCGG_y
Cuts at: 2680 2861 3072 2680
Size: 181 211 4292
Fragments arranged by size:
4292 211 181
BssSI C'ACGA_G
Cuts at: 294 2953 3313 294
Size: 2659 360 1665
Fragments arranged by size:
2659 1665 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2059 2059
Size: 4684
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4684
BstXI CCAn_nnnn'nTGG
Cuts at: 246 246
Size: 4684
BstYI r'GATC_y
Cuts at: 1 428 1707 2174 2197 2532 2778 3792 3878
Size: 427 1279 467 23 335 246 1014 86
Cuts at: 3878 3890 4236 1
Size: 12 346 449
Fragments arranged by size:
1279 1014 467 449 427 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 595 677 595
Size: 82 4602
BtgI C'CryG_G
Cuts at: 282 315 945 1421 282
Size: 33 630 476 3545
Fragments arranged by size:
3545 630 476 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 846 2632 2813 2976 846
Size: 1786 181 163 2554
Fragments arranged by size:
2554 1786 181 163
BtsI GCAGTG_nn'
Cuts at: 474 538 1145 1925 474
Size: 64 607 780 3233
Fragments arranged by size:
3233 780 607 64
DraI TTT'AAA
Cuts at: 1681 1681
Size: 4684
DrdI GACnn_nn'nnGTC
Cuts at: 201 2388 3248 201
Size: 2187 860 1637
Fragments arranged by size:
2187 1637 860
EaeI y'GGCC_r
Cuts at: 398 612 2267 2441 2832 2859 4206 398
Size: 214 1655 174 391 27 1347 876
Fragments arranged by size:
1655 1347 876 391 214 174 27
EagI C'GGCC_G
Cuts at: 2267 2267
Size: 4684
EarI CTCTTCn'nnn_
Cuts at: 164 847 889 1116 2705 2915 164
Size: 683 42 227 1589 210 1933
Fragments arranged by size:
1933 1589 683 227 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2890 3202 3348 4494 2890
Size: 312 146 1146 3080
Fragments arranged by size:
3080 1146 312 146
EcoICRI GAG'CTC
Cuts at: 157 670 1694 157
Size: 513 1024 3147
Fragments arranged by size:
3147 1024 513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 1171 1464 1489 1590 2507 2939 3688 3956
Size: 964 293 25 101 917 432 749 268
Cuts at: 3956 207
Size: 935
Fragments arranged by size:
964 935 917 749 432 293 268 101
25
EcoNI CCTnn'n_nnAGG
Cuts at: 814 1608 814
Size: 794 3890
EcoO109I rG'GnC_Cy
Cuts at: 175 277 311 457 681 697 1441 1821 175
Size: 102 34 146 224 16 744 380 3038
Fragments arranged by size:
3038 744 380 224 146 102 34 16
EcoRI G'AATT_C
Cuts at: 1686 1686
Size: 4684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 2701 348
Size: 2353 2331
FspI TGC'GCA
Cuts at: 2463 2463
Size: 4684
HaeII r_GCGC'y
Cuts at: 135 2116 2364 3388 4367 135
Size: 1981 248 1024 979 452
Fragments arranged by size:
1981 1024 979 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 167 199 288 320 367 399 536 568 1093
Size: 32 89 32 47 32 137 32 525
Cuts at: 1093 1125 2524 2554 2556 2586 167
Size: 32 1399 30 2 30 2265
Fragments arranged by size:
2265 1399 525 137 89 47 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1721 1721
Size: 4684
HindIII A'AGCT_T
Cuts at: 1187 1187
Size: 4684
KasI G'GCGC_C
Cuts at: 131 2112 2360 131
Size: 1981 248 2455
Fragments arranged by size:
2455 1981 248
KpnI G_GTAC'C
Cuts at: 1293 1702 1293
Size: 409 4275
MluI A'CGCG_T
Cuts at: 4117 4117
Size: 4684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3355 3539 3355
Size: 184 4500
MscI TGG'CCA
Cuts at: 614 2443 614
Size: 1829 2855
MslI CAynn'nnrTG
Cuts at: 989 2671 2798 989
Size: 1682 127 2875
Fragments arranged by size:
2875 1682 127
MspA1I CmG'CkG
Cuts at: 417 465 947 1793 2107 2467 3482 3727 417
Size: 48 482 846 314 360 1015 245 1374
Fragments arranged by size:
1374 1015 846 482 360 314 245 48
NaeI GCC'GGC
Cuts at: 2863 2863
Size: 4684
NarI GG'CG_CC
Cuts at: 132 2113 2361 132
Size: 1981 248 2455
Fragments arranged by size:
2455 1981 248
NcoI C'CATG_G
Cuts at: 282 315 1421 282
Size: 33 1106 3545
Fragments arranged by size:
3545 1106 33
NgoMIV G'CCGG_C
Cuts at: 2861 2861
Size: 4684
NheI G'CTAG_C
Cuts at: 1512 1512
Size: 4684
NruI TCG'CGA
Cuts at: 3045 3045
Size: 4684
NspI r_CATG'y
Cuts at: 724 1540 1735 2235 3144 724
Size: 816 195 500 909 2264
Fragments arranged by size:
2264 909 816 500 195
PasI CC'CwG_GG
Cuts at: 1007 1007
Size: 4684
PciI A'CATG_T
Cuts at: 3140 3140
Size: 4684
PflMI CCAn_nnn'nTGG
Cuts at: 386 1065 386
Size: 679 4005
PmeI GTTT'AAAC
Cuts at: 1681 1681
Size: 4684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3823 3855 3823
Size: 32 4652
PpuMI rG'GwC_Cy
Cuts at: 175 277 311 457 681 697 175
Size: 102 34 146 224 16 4162
Fragments arranged by size:
4162 224 146 102 34 16
PshAI GACnn'nnGTC
Cuts at: 1470 1470
Size: 4684
PspOMI G'GGCC_C
Cuts at: 1441 1441
Size: 4684
PspXI vC'TCGA_Gb
Cuts at: 1542 1542
Size: 4684
PstI C_TGCA'G
Cuts at: 416 942 1164 1434 1498 1573 1729 2414 416
Size: 526 222 270 64 75 156 685 2686
Fragments arranged by size:
2686 685 526 270 222 156 75 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4684
PvuII CAG'CTG
Cuts at: 417 2107 2467 417
Size: 1690 360 2634
Fragments arranged by size:
2634 1690 360
RsrII CG'GwC_CG
Cuts at: 2877 2877
Size: 4684
SacI G_AGCT'C
Cuts at: 159 672 1696 159
Size: 513 1024 3147
Fragments arranged by size:
3147 1024 513
SacII CC_GC'GG
Cuts at: 948 948
Size: 4684
SalI G'TCGA_C
Cuts at: 1719 1719
Size: 4684
SapI GCTCTTCn'nnn_
Cuts at: 164 847 2705 2915 164
Size: 683 1858 210 1933
Fragments arranged by size:
1933 1858 683 210
SbfI CC_TGCA'GG
Cuts at: 1729 1729
Size: 4684
ScaI AGT'ACT
Cuts at: 4201 4201
Size: 4684
SfcI C'TryA_G
Cuts at: 33 412 938 951 1160 1430 1494 1569 1725
Size: 379 526 13 209 270 64 75 156
Cuts at: 1725 2410 3405 3596 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 526 379 270 209 191
156 75 64 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 406 406
Size: 4684
SfoI GGC'GCC
Cuts at: 133 2114 2362 133
Size: 1981 248 2455
Fragments arranged by size:
2455 1981 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4684
SmaI CCC'GGG
Cuts at: 140 1439 1704 140
Size: 1299 265 3120
Fragments arranged by size:
3120 1299 265
SmlI C'TyrA_G
Cuts at: 811 1173 1350 1542 1837 2752 3246 3508 811
Size: 362 177 192 295 915 494 262 1987
Fragments arranged by size:
1987 915 494 362 295 262 192 177
SnaBI TAC'GTA
Cuts at: 1674 1674
Size: 4684
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4684
SphI G_CATG'C
Cuts at: 724 1735 2235 724
Size: 1011 500 3173
Fragments arranged by size:
3173 1011 500
StuI AGG'CCT
Cuts at: 1319 1348 1319
Size: 29 4655
StyI C'CwwG_G
Cuts at: 282 307 315 1211 1296 1320 1421 1557 1816
Size: 25 8 896 85 24 101 136 259
Cuts at: 1816 2170 282
Size: 354 2796
Fragments arranged by size:
2796 896 354 259 136 101 85 25
24 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 542 2265 2398 2857 3068 542
Size: 1723 133 459 211 2158
Fragments arranged by size:
2158 1723 459 211 133
TatI w'GTAC_w
Cuts at: 965 4199 965
Size: 3234 1450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 4201 4233 517
Size: 32 3652 32 968
Fragments arranged by size:
3652 968 32 32
Tth111I GACn'n_nGTC
Cuts at: 2479 2479
Size: 4684
XbaI T'CTAG_A
Cuts at: 1713 1713
Size: 4684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1112 2177 1112
Size: 1065 3619
XhoI C'TCGA_G
Cuts at: 1542 1542
Size: 4684
XmaI C'CCGG_G
Cuts at: 138 1437 1702 138
Size: 1299 265 3120
Fragments arranged by size:
3120 1299 265
XmnI GAAnn'nnTTC
Cuts at: 1474 1474
Size: 4684
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NotI NsiI PacI
PfoI PmlI PsiI PsrI SanDI SexAI SgrAI SrfI
SspI SwaI ZraI