Restriction map for
pF1KSDA0147
(Circular) (Six-base) MAPSORT of: pF1KSDA0147.seq Check: 7694 from: 1 to: 8027
pF1KSDA0147 8027 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1369 1369
Size: 8027
AccI GT'mk_AC
Cuts at: 944 5065 944
Size: 4121 3906
Acc65I G'GTAC_C
Cuts at: 3972 5043 3972
Size: 1071 6956
AclI AA'CG_TT
Cuts at: 7867 7867
Size: 8027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 940 2192 2730 3937 4964 5852 6284 7031
Size: 496 1252 538 1207 1027 888 432 747
Cuts at: 7031 7299 444
Size: 268 1172
Fragments arranged by size:
1252 1207 1172 1027 888 747 538 496
432 268
AfeI AGC'GCT
Cuts at: 3954 3954
Size: 8027
AflIII A'CryG_T
Cuts at: 389 803 5379 6483 7460 389
Size: 414 4576 1104 977 956
Fragments arranged by size:
4576 1104 977 956 414
AgeI A'CCGG_T
Cuts at: 6415 6415
Size: 8027
AhdI GACnn_n'nnGTC
Cuts at: 1632 1632
Size: 8027
AleI CACnn'nnGTG
Cuts at: 2542 4774 2542
Size: 2232 5795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2560 3295 2560
Size: 735 7292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 460 492 3416 3448 460
Size: 32 2924 32 5039
Fragments arranged by size:
5039 2924 32 32
AlwNI CAG_nnn'CTG
Cuts at: 552 885 1242 1476 1630 1720 3618 3864 3915
Size: 333 357 234 154 90 1898 246 51
Cuts at: 3915 4335 4354 5332 6899 552
Size: 420 19 978 1567 1680
Fragments arranged by size:
1898 1680 1567 978 420 357 333 246
234 154 90 51 19
ApaI G_GGCC'C
Cuts at: 1741 2041 2097 2693 2983 3163 3832 3878 4243
Size: 300 56 596 290 180 669 46 365
Cuts at: 4243 4602 4721 4942 5005 1741
Size: 359 119 221 63 4763
Fragments arranged by size:
4763 669 596 365 359 300 290 221
180 119 63 56 46
ApaLI G'TGCA_C
Cuts at: 6797 6797
Size: 8027
ApoI r'AATT_y
Cuts at: 5031 7380 7636 5031
Size: 2349 256 5422
Fragments arranged by size:
5422 2349 256
AseI AT'TA_AT
Cuts at: 20 2879 20
Size: 2859 5168
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8027
AvaI C'yCGr_G
Cuts at: 352 556 772 1096 1111 1396 1732 1858 1990
Size: 204 216 324 15 285 336 126 132
Cuts at: 1990 2050 2090 2408 2435 2614 2633 2686 3061
Size: 60 40 318 27 179 19 53 375
Cuts at: 3061 3411 3877 4437 4567 4809 5047 352
Size: 350 466 560 130 242 238 3332
Fragments arranged by size:
3332 560 466 375 350 336 324 318
285 242 238 216 204 179 132 130
126 60 53 40 27 19 15
BamHI G'GATC_C
Cuts at: 5052 5052
Size: 8027
BanI G'GyrC_C
Cuts at: 785 2494 3066 3405 3441 3478 3972 4489 4520
Size: 1709 572 339 36 37 494 517 31
Cuts at: 4520 4563 4988 5043 5457 5705 5740 785
Size: 43 425 55 414 248 35 3072
Fragments arranged by size:
3072 1709 572 517 494 425 414 339
248 55 43 37 36 35 31
BanII G_rGCy'C
Cuts at: 599 711 773 842 1568 1733 1741 1826 2041
Size: 112 62 69 726 165 8 85 215
Cuts at: 2041 2088 2097 2368 2693 2983 3163 3547 3823
Size: 47 9 271 325 290 180 384 276
Cuts at: 3823 3832 3878 4243 4444 4462 4595 4602 4721
Size: 9 46 365 201 18 133 7 119
Cuts at: 4721 4942 5005 5041 6071 599
Size: 221 63 36 1030 2555
Fragments arranged by size:
2555 1030 726 384 365 325 290 276
271 221 215 201 180 165 133 119
112 85 69 63 62 47 46 36
18 9 9 8 7
BbeI G_GCGC'C
Cuts at: 789 2498 4493 4524 4992 5461 5709 789
Size: 1709 1995 31 468 469 248 3107
Fragments arranged by size:
3107 1995 1709 469 468 248 31
BbsI GAAGACnn'nnnn_
Cuts at: 1697 1805 4787 1697
Size: 108 2982 4937
Fragments arranged by size:
4937 2982 108
BbvCI CC'TCA_GC
Cuts at: 1505 1748 4684 4835 1505
Size: 243 2936 151 4697
Fragments arranged by size:
4697 2936 243 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1185 1219 2809 2843 4600 4634 1185
Size: 34 1590 34 1757 34 4578
Fragments arranged by size:
4578 1757 1590 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5925 6686 8027 5925
Size: 761 1341 5925
Fragments arranged by size:
5925 1341 761
BclI T'GATC_A
Cuts at: 5547 5547
Size: 8027
BglI GCCn_nnn'nGGC
Cuts at: 2002 2518 2610 2719 3494 3623 3827 4090 4099
Size: 516 92 109 775 129 204 263 9
Cuts at: 4099 4606 2002
Size: 507 5423
Fragments arranged by size:
5423 775 516 507 263 204 129 109
92 9
BglII A'GATC_T
Cuts at: 215 1970 5542 7579 215
Size: 1755 3572 2037 663
Fragments arranged by size:
3572 2037 1755 663
BlpI GC'TnA_GC
Cuts at: 836 1727 3817 5139 836
Size: 891 2090 1322 3724
Fragments arranged by size:
3724 2090 1322 891
Bme1580I G_kGCm'C
Cuts at: 1741 2041 2097 2693 2848 2983 3163 3357 3832
Size: 300 56 596 155 135 180 194 475
Cuts at: 3832 3878 3950 4109 4229 4243 4578 4602 4711
Size: 46 72 159 120 14 335 24 109
Cuts at: 4711 4721 4942 5005 5012 5652 5745 6801 1741
Size: 10 221 63 7 640 93 1056 2967
Fragments arranged by size:
2967 1056 640 596 475 335 300 221
194 180 159 155 135 120 109 93
72 63 56 46 24 14 10 7
BmgBI CAC'GTC
Cuts at: 390 564 804 1218 2892 3321 390
Size: 174 240 414 1674 429 5096
Fragments arranged by size:
5096 1674 429 414 240 174
BmrI ACTGGGnnnn_n'
Cuts at: 2339 3121 3879 5239 5320 5655 7799 2339
Size: 782 758 1360 81 335 2144 2567
Fragments arranged by size:
2567 2144 1360 782 758 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1014 3813 6394 1014
Size: 2799 2581 2647
Fragments arranged by size:
2799 2647 2581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 462 466 613 841 1455 1498 2462 2488
Size: 203 4 147 228 614 43 964 26
Cuts at: 2488 3394 3745 3808 4001 4010 4234 4624 4744
Size: 906 351 63 193 9 224 390 120
Cuts at: 4744 259
Size: 3542
Fragments arranged by size:
3542 964 906 614 390 351 228 224
203 193 147 120 63 43 26 9
4
Bpu10I CC'TnA_GC
Cuts at: 322 1505 1748 4247 4684 4835 322
Size: 1183 243 2499 437 151 3514
Fragments arranged by size:
3514 2499 1183 437 243 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 598 1170 1255 1552 4434 5203 6082 6574
Size: 483 572 85 297 2882 769 879 492
Cuts at: 6574 6872 115
Size: 298 1270
Fragments arranged by size:
2882 1270 879 769 572 492 483 298
297 85
BsaI GGTCTCn'nnnn_
Cuts at: 1423 7731 1423
Size: 6308 1719
BsaAI yAC'GTr
Cuts at: 165 1209 2709 5019 5380 165
Size: 1044 1500 2310 361 2812
Fragments arranged by size:
2812 2310 1500 1044 361
BsaBI GATnn'nnATC
Cuts at: 6 5541 7578 6
Size: 5535 2037 455
Fragments arranged by size:
5535 2037 455
BsaHI Gr'CG_yC
Cuts at: 257 786 1802 2495 4465 4490 4521 4815 4989
Size: 529 1016 693 1970 25 31 294 174
Cuts at: 4989 5458 5706 6408 7904 257
Size: 469 248 702 1496 380
Fragments arranged by size:
1970 1496 1016 702 693 529 469 380
294 248 174 31 25
BsaWI w'CCGG_w
Cuts at: 740 1085 1313 3776 4053 4551 4824 5216 5737
Size: 345 228 2463 277 498 273 392 521
Cuts at: 5737 6415 6689 6836 7247 740
Size: 678 274 147 411 1520
Fragments arranged by size:
2463 1520 678 521 498 411 392 345
277 274 273 228 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 460 490 2435 2465 2876 2905 2906 2935 4165
Size: 30 1945 30 411 29 1 29 1230
Cuts at: 4165 4195 4831 4861 460
Size: 30 636 30 3626
Fragments arranged by size:
3626 1945 1230 636 411 30 30 30
30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 256 838 1596 1666 1669 1894 2078 2119
Size: 30 582 758 70 3 225 184 41
Cuts at: 2119 2122 2125 2128 2152 2155 2164 2167 2170
Size: 3 3 3 24 3 9 3 3
Cuts at: 2170 2209 2212 2308 3148 4309 4414 4852 4897
Size: 39 3 96 840 1161 105 438 45
Cuts at: 4897 226
Size: 3356
Fragments arranged by size:
3356 1161 840 758 582 438 225 184
105 96 70 45 41 39 30 24
9 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 315 1376 1405 1558 1985 2739 2974 2992 3091
Size: 1061 29 153 427 754 235 18 99
Cuts at: 3091 3154 3456 3621 3949 4139 4354 4392 4864
Size: 63 302 165 328 190 215 38 472
Cuts at: 4864 5452 6210 6787 7283 315
Size: 588 758 577 496 1059
Fragments arranged by size:
1061 1059 758 754 588 577 496 472
427 328 302 235 215 190 165 153
99 63 38 29 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2566 3667 3850 3985 4564 4980 1189
Size: 1377 1101 183 135 579 416 4236
Fragments arranged by size:
4236 1377 1101 579 416 183 135
BsiEI CG_ry'CG
Cuts at: 104 2865 3573 3810 4103 5569 5615 6823 104
Size: 2761 708 237 293 1466 46 1208 1308
Fragments arranged by size:
2761 1466 1308 1208 708 293 237 46
BsiHKAI G_wGCw'C
Cuts at: 599 773 842 1358 1568 2504 3251 4595 4674
Size: 174 69 516 210 936 747 1344 79
Cuts at: 4674 5041 5819 6009 6801 599
Size: 367 778 190 792 1825
Fragments arranged by size:
1825 1344 936 792 778 747 516 367
210 190 174 79 69
BsmBI CGTCTCn'nnnn_
Cuts at: 2811 2925 4381 2811
Size: 114 1456 6457
Fragments arranged by size:
6457 1456 114
Bsp1286I G_dGCh'C
Cuts at: 599 711 773 842 1358 1568 1733 1741 1826
Size: 112 62 69 516 210 165 8 85
Cuts at: 1826 2041 2088 2097 2368 2504 2693 2848 2983
Size: 215 47 9 271 136 189 155 135
Cuts at: 2983 3163 3251 3357 3547 3823 3832 3878 3950
Size: 180 88 106 190 276 9 46 72
Cuts at: 3950 4109 4229 4243 4444 4462 4578 4595 4602
Size: 159 120 14 201 18 116 17 7
Cuts at: 4602 4674 4711 4721 4942 5005 5012 5041 5652
Size: 72 37 10 221 63 7 29 611
Cuts at: 5652 5745 5819 6009 6071 6801 599
Size: 93 74 190 62 730 1825
Fragments arranged by size:
1825 730 611 516 276 271 221 215
210 201 190 190 189 180 165 159
155 136 135 120 116 112 106 93
88 85 74 72 72 69 63 62
62 47 46 37 29 18 17 14
10 9 9 8 7 7
BspEI T'CCGG_A
Cuts at: 4053 4551 7247 4053
Size: 498 2696 4833
Fragments arranged by size:
4833 2696 498
BspHI T'CATG_A
Cuts at: 7203 7203
Size: 8027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 617 788 875 893 1031 1369 2276 2844 3886
Size: 171 87 18 138 338 907 568 1042
Cuts at: 3886 4286 4679 5077 5291 5593 5974 617
Size: 400 393 398 214 302 381 2670
Fragments arranged by size:
2670 1042 907 568 400 398 393 381
338 302 214 171 138 87 18
BsrBI CCG'CTC
Cuts at: 50 1043 2640 2967 3268 4194 4533 6319 6373
Size: 993 1597 327 301 926 339 1786 54
Cuts at: 6373 7857 8022 50
Size: 1484 165 55
Fragments arranged by size:
1786 1597 1484 993 926 339 327 301
165 55 54
BsrDI GCAATG_nn'
Cuts at: 2348 2750 2813 3743 5939 2348
Size: 402 63 930 2196 4436
Fragments arranged by size:
4436 2196 930 402 63
BsrFI r'CCGG_y
Cuts at: 158 815 1342 2533 4560 6025 6206 6415 158
Size: 657 527 1191 2027 1465 181 209 1770
Fragments arranged by size:
2027 1770 1465 1191 657 527 209 181
BssSI C'ACGA_G
Cuts at: 2506 6298 6656 2506
Size: 3792 358 3877
Fragments arranged by size:
3877 3792 358
BstAPI GCAn_nnn'nTGC
Cuts at: 204 552 1242 1308 2019 3321 3352 3618 4335
Size: 348 690 66 711 1302 31 266 717
Cuts at: 4335 5404 204
Size: 1069 2827
Fragments arranged by size:
2827 1302 1069 717 711 690 348 266
66 31
BstBI TT'CG_AA
Cuts at: 110 6388 110
Size: 6278 1749
BstEII G'GTnAC_C
Cuts at: 585 2592 585
Size: 2007 6020
BstXI CCAn_nnnn'nTGG
Cuts at: 3099 3211 3226 4776 3099
Size: 112 15 1550 6350
Fragments arranged by size:
6350 1550 112 15
BstYI r'GATC_y
Cuts at: 1 215 335 413 663 1970 3137 5052 5519
Size: 214 120 78 250 1307 1167 1915 467
Cuts at: 5519 5542 5877 6123 7135 7221 7233 7579 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1915 1307 1167 1012 467 449 346 335
250 246 214 120 86 78 23 12
Bsu36I CC'TnA_GG
Cuts at: 1892 2132 3824 4124 1892
Size: 240 1692 300 5795
Fragments arranged by size:
5795 1692 300 240
BtgI C'CryG_G
Cuts at: 1826 2216 2513 3253 3471 3548 4992 1826
Size: 390 297 740 218 77 1444 4861
Fragments arranged by size:
4861 1444 740 390 297 218 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1454 2934 5977 6158 6321 1454
Size: 1480 3043 181 163 3160
Fragments arranged by size:
3160 3043 1480 181 163
BtsI GCAGTG_nn'
Cuts at: 550 757 1849 2537 2848 3050 3311 3666 5270
Size: 207 1092 688 311 202 261 355 1604
Cuts at: 5270 550
Size: 3307
Fragments arranged by size:
3307 1604 1092 688 355 311 261 207
202
DraI TTT'AAA
Cuts at: 5026 5026
Size: 8027
DraIII CAC_nnn'GTG
Cuts at: 1759 1759
Size: 8027
DrdI GACnn_nn'nnGTC
Cuts at: 2917 5733 6591 2917
Size: 2816 858 4353
Fragments arranged by size:
4353 2816 858
EaeI y'GGCC_r
Cuts at: 359 1166 3263 3771 3938 4026 4100 4505 4733
Size: 807 2097 508 167 88 74 405 228
Cuts at: 4733 5612 5786 6177 6204 7549 359
Size: 879 174 391 27 1345 837
Fragments arranged by size:
2097 1345 879 837 807 508 405 391
228 174 167 88 74 27
EagI C'GGCC_G
Cuts at: 4100 5612 4100
Size: 1512 6515
EarI CTCTTCn'nnn_
Cuts at: 1573 1802 2114 2141 2168 2174 2294 2990 3804
Size: 229 312 27 27 6 120 696 814
Cuts at: 3804 4315 6050 6260 1573
Size: 511 1735 210 3340
Fragments arranged by size:
3340 1735 814 696 511 312 229 210
120 27 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1614 6235 6545 6691 7837 1614
Size: 4621 310 146 1146 1804
Fragments arranged by size:
4621 1804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 597 771 840 1566 4593 5039 597
Size: 174 69 726 3027 446 3585
Fragments arranged by size:
3585 3027 726 446 174 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 444 462 466 613 841 940 1455 1498
Size: 185 18 4 147 228 99 515 43
Cuts at: 1498 2192 2462 2488 2730 3394 3745 3808 3937
Size: 694 270 26 242 664 351 63 129
Cuts at: 3937 4001 4010 4234 4624 4744 4964 5852 6284
Size: 64 9 224 390 120 220 888 432
Cuts at: 6284 7031 7299 259
Size: 747 268 987
Fragments arranged by size:
987 888 747 694 664 515 432 390
351 270 268 242 228 224 220 185
147 129 120 99 64 63 43 26
18 9 4
EcoO109I rG'GnC_Cy
Cuts at: 1275 1482 1530 1737 1896 2037 2038 2334 2425
Size: 207 48 207 159 141 1 296 91
Cuts at: 2425 2690 2979 2980 3159 3160 3258 3399 3828
Size: 265 289 1 179 1 98 141 429
Cuts at: 3828 3874 4091 4239 4240 4599 4691 4717 4718
Size: 46 217 148 1 359 92 26 1
Cuts at: 4718 4740 4847 4919 4939 5002 5166 1275
Size: 22 107 72 20 63 164 4136
Fragments arranged by size:
4136 429 359 296 289 265 217 207
207 179 164 159 148 141 141 107
98 92 91 72 63 48 46 26
22 20 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 5031 5031
Size: 8027
EcoRV GAT'ATC
Cuts at: 405 405
Size: 8027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6046 6046
Size: 8027
FspI TGC'GCA
Cuts at: 310 508 5808 310
Size: 198 5300 2529
Fragments arranged by size:
5300 2529 198
HaeII r_GCGC'y
Cuts at: 153 789 1105 1542 2498 2524 2706 3956 4493
Size: 636 316 437 956 26 182 1250 537
Cuts at: 4493 4524 4934 4992 5461 5709 6731 7710 153
Size: 31 410 58 469 248 1022 979 470
Fragments arranged by size:
1250 1022 979 956 636 537 470 469
437 410 316 248 182 58 31 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1650 1726 1758 4165 4197 4831 4863 5869
Size: 32 76 32 2407 32 634 32 1006
Cuts at: 5869 5899 5901 5931 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 2407 1006 634 76 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 5066 5066
Size: 8027
KasI G'GCGC_C
Cuts at: 785 2494 4489 4520 4988 5457 5705 785
Size: 1709 1995 31 468 469 248 3107
Fragments arranged by size:
3107 1995 1709 469 468 248 31
KpnI G_GTAC'C
Cuts at: 3976 5047 3976
Size: 1071 6956
MluI A'CGCG_T
Cuts at: 7460 7460
Size: 8027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 788 2942 3092 3162 3215 3578 6698 6882 788
Size: 2154 150 70 53 363 3120 184 1933
Fragments arranged by size:
3120 2154 1933 363 184 150 70 53
MscI TGG'CCA
Cuts at: 361 4028 5788 361
Size: 3667 1760 2600
Fragments arranged by size:
3667 2600 1760
MslI CAynn'nnrTG
Cuts at: 2542 4774 6016 6143 2542
Size: 2232 1242 127 4426
Fragments arranged by size:
4426 2232 1242 127
MspA1I CmG'CkG
Cuts at: 144 267 342 375 464 504 660 750 918
Size: 123 75 33 89 40 156 90 168
Cuts at: 918 1519 1926 2607 2935 3025 3255 3275 3473
Size: 601 407 681 328 90 230 20 198
Cuts at: 3473 3651 3988 4179 5138 5452 5812 6825 7070
Size: 178 337 191 959 314 360 1013 245
Cuts at: 7070 144
Size: 1101
Fragments arranged by size:
1101 1013 959 681 601 407 360 337
328 314 245 230 198 191 178 168
156 123 90 90 89 75 40 33
20
NaeI GCC'GGC
Cuts at: 2535 6208 2535
Size: 3673 4354
NarI GG'CG_CC
Cuts at: 786 2495 4490 4521 4989 5458 5706 786
Size: 1709 1995 31 468 469 248 3107
Fragments arranged by size:
3107 1995 1709 469 468 248 31
NgoMIV G'CCGG_C
Cuts at: 2533 6206 2533
Size: 3673 4354
NspI r_CATG'y
Cuts at: 2022 5080 5580 6487 2022
Size: 3058 500 907 3562
Fragments arranged by size:
3562 3058 907 500
PasI CC'CwG_GG
Cuts at: 1047 2081 4427 4479 1047
Size: 1034 2346 52 4595
Fragments arranged by size:
4595 2346 1034 52
PciI A'CATG_T
Cuts at: 6483 6483
Size: 8027
PflMI CCAn_nnn'nTGG
Cuts at: 1476 1630 3154 1476
Size: 154 1524 6349
Fragments arranged by size:
6349 1524 154
PfoI T'CCnGG_A
Cuts at: 394 416 599 1475 2465 3218 4187 394
Size: 22 183 876 990 753 969 4234
Fragments arranged by size:
4234 990 969 876 753 183 22
PmeI GTTT'AAAC
Cuts at: 5026 5026
Size: 8027
PmlI CAC'GTG
Cuts at: 165 1209 2709 165
Size: 1044 1500 5483
Fragments arranged by size:
5483 1500 1044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 460 492 7166 7198 460
Size: 32 6674 32 1289
Fragments arranged by size:
6674 1289 32 32
PpuMI rG'GwC_Cy
Cuts at: 3399 4691 4847 3399
Size: 1292 156 6579
Fragments arranged by size:
6579 1292 156
PshAI GACnn'nnGTC
Cuts at: 4818 4818
Size: 8027
PspOMI G'GGCC_C
Cuts at: 1737 2037 2093 2689 2979 3159 3828 3874 4239
Size: 300 56 596 290 180 669 46 365
Cuts at: 4239 4598 4717 4938 5001 1737
Size: 359 119 221 63 4763
Fragments arranged by size:
4763 669 596 365 359 300 290 221
180 119 63 56 46
PspXI vC'TCGA_Gb
Cuts at: 1096 1396 1096
Size: 300 7727
PstI C_TGCA'G
Cuts at: 200 548 1238 1518 1650 2015 2492 2855 3911
Size: 348 690 280 132 365 477 363 1056
Cuts at: 3911 4164 5074 5759 200
Size: 253 910 685 2468
Fragments arranged by size:
2468 1056 910 690 685 477 365 363
348 280 253 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8027
PvuII CAG'CTG
Cuts at: 267 375 504 660 750 918 1519 3651 3988
Size: 108 129 156 90 168 601 2132 337
Cuts at: 3988 4179 5452 5812 267
Size: 191 1273 360 2482
Fragments arranged by size:
2482 2132 1273 601 360 337 191 168
156 129 108 90
RsrII CG'GwC_CG
Cuts at: 1340 6222 1340
Size: 4882 3145
SacI G_AGCT'C
Cuts at: 599 773 842 1568 4595 5041 599
Size: 174 69 726 3027 446 3585
Fragments arranged by size:
3585 3027 726 446 174 69
SacII CC_GC'GG
Cuts at: 3256 3474 3256
Size: 218 7809
SalI G'TCGA_C
Cuts at: 5064 5064
Size: 8027
SanDI GG'GwC_CC
Cuts at: 3399 3399
Size: 8027
SapI GCTCTTCn'nnn_
Cuts at: 2294 6050 6260 2294
Size: 3756 210 4061
Fragments arranged by size:
4061 3756 210
SbfI CC_TGCA'GG
Cuts at: 5074 5074
Size: 8027
ScaI AGT'ACT
Cuts at: 4204 7544 4204
Size: 3340 4687
SexAI A'CCwGG_T
Cuts at: 581 581
Size: 8027
SfcI C'TryA_G
Cuts at: 33 196 225 544 1234 1514 1534 1646 2011
Size: 163 29 319 690 280 20 112 365
Cuts at: 2011 2488 2778 2851 3907 4086 4160 4312 5070
Size: 477 290 73 1056 179 74 152 758
Cuts at: 5070 5755 6748 6939 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1056 993 758 690 685 477 365
319 290 280 191 179 163 152 112
74 73 29 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 4090 4099 4606 4090
Size: 9 507 7511
Fragments arranged by size:
7511 507 9
SfoI GGC'GCC
Cuts at: 787 2496 4491 4522 4990 5459 5707 787
Size: 1709 1995 31 468 469 248 3107
Fragments arranged by size:
3107 1995 1709 469 468 248 31
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8027
SmaI CCC'GGG
Cuts at: 558 1860 2052 2437 2688 3063 3413 4811 5049
Size: 1302 192 385 251 375 350 1398 238
Cuts at: 5049 558
Size: 3536
Fragments arranged by size:
3536 1398 1302 385 375 350 251 238
192
SmlI C'TyrA_G
Cuts at: 130 613 1096 1149 1270 1396 1567 4449 5182
Size: 483 483 53 121 126 171 2882 733
Cuts at: 5182 6097 6589 6851 130
Size: 915 492 262 1306
Fragments arranged by size:
2882 1306 915 733 492 483 483 262
171 126 121 53
SnaBI TAC'GTA
Cuts at: 5019 5019
Size: 8027
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8027
SphI G_CATG'C
Cuts at: 2022 5080 5580 2022
Size: 3058 500 4469
Fragments arranged by size:
4469 3058 500
SrfI GCCC'GGGC
Cuts at: 2437 2437
Size: 8027
StuI AGG'CCT
Cuts at: 1609 4084 4983 1609
Size: 2475 899 4653
Fragments arranged by size:
4653 2475 899
StyI C'CwwG_G
Cuts at: 3242 3536 4785 5161 5515 3242
Size: 294 1249 376 354 5754
Fragments arranged by size:
5754 1249 376 354 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 901 1273 2443 4255 5610 5743 6202 6411 901
Size: 372 1170 1812 1355 133 459 209 2517
Fragments arranged by size:
2517 1812 1355 1170 459 372 209 133
TatI w'GTAC_w
Cuts at: 4202 7542 4202
Size: 3340 4687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1124 1156 2435 2467 2876 2905 2908 2937 4664
Size: 32 1279 32 409 29 3 29 1727
Cuts at: 4664 4696 7544 7576 1124
Size: 32 2848 32 1575
Fragments arranged by size:
2848 1727 1575 1279 409 32 32 32
32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 2866 5824 2866
Size: 2958 5069
XbaI T'CTAG_A
Cuts at: 5058 5058
Size: 8027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1077 1627 3442 5522 1077
Size: 550 1815 2080 3582
Fragments arranged by size:
3582 2080 1815 550
XhoI C'TCGA_G
Cuts at: 1096 1396 1096
Size: 300 7727
XmaI C'CCGG_G
Cuts at: 556 1858 2050 2435 2686 3061 3411 4809 5047
Size: 1302 192 385 251 375 350 1398 238
Cuts at: 5047 556
Size: 3536
Fragments arranged by size:
3536 1398 1302 385 375 350 251 238
192
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BfrBI BmtI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PsiI PsrI SgrAI SspI SwaI
XmnI ZraI