Restriction map for
pF1KSDA0149
(Circular) (Six-base) MAPSORT of: pF1KSDA0149.seq Check: 663 from: 1 to: 5627
pF1KSDA0149 5627 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 847 1798 847
Size: 951 4676
AccI GT'mk_AC
Cuts at: 2665 2665
Size: 5627
Acc65I G'GTAC_C
Cuts at: 1159 1909 2643 1159
Size: 750 734 4143
Fragments arranged by size:
4143 750 734
AclI AA'CG_TT
Cuts at: 5467 5467
Size: 5627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 3452 3884 4631 4899 2570
Size: 882 432 747 268 3298
Fragments arranged by size:
3298 882 747 432 268
AfeI AGC'GCT
Cuts at: 1108 1108
Size: 5627
AflIII A'CryG_T
Cuts at: 2979 4083 5060 2979
Size: 1104 977 3546
Fragments arranged by size:
3546 1104 977
AgeI A'CCGG_T
Cuts at: 2105 4015 2105
Size: 1910 3717
AleI CACnn'nnGTG
Cuts at: 212 917 212
Size: 705 4922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1426 1426
Size: 5627
AlwNI CAG_nnn'CTG
Cuts at: 1093 1372 2098 2137 2331 2509 2932 4499 1093
Size: 279 726 39 194 178 423 1567 2221
Fragments arranged by size:
2221 1567 726 423 279 194 178 39
ApaI G_GGCC'C
Cuts at: 378 415 1133 1262 1467 2185 378
Size: 37 718 129 205 718 3820
Fragments arranged by size:
3820 718 718 205 129 37
ApaLI G'TGCA_C
Cuts at: 834 4397 834
Size: 3563 2064
ApoI r'AATT_y
Cuts at: 2631 4980 5236 2631
Size: 2349 256 3022
Fragments arranged by size:
3022 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5627
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
AvaI C'yCGr_G
Cuts at: 167 396 1874 1985 2056 2083 2178 2647 167
Size: 229 1478 111 71 27 95 469 3147
Fragments arranged by size:
3147 1478 469 229 111 95 71 27
BamHI G'GATC_C
Cuts at: 2224 2532 2652 2224
Size: 308 120 5199
Fragments arranged by size:
5199 308 120
BanI G'GyrC_C
Cuts at: 655 686 1024 1159 1909 2473 2643 3057 3305
Size: 31 338 135 750 564 170 414 248
Cuts at: 3305 3340 655
Size: 35 2942
Fragments arranged by size:
2942 750 564 414 338 248 170 135
35 31
BanII G_rGCy'C
Cuts at: 378 415 507 1133 1262 1467 1561 1952 2179
Size: 37 92 626 129 205 94 391 227
Cuts at: 2179 2185 2386 2496 2641 3671 378
Size: 6 201 110 145 1030 2334
Fragments arranged by size:
2334 1030 626 391 227 205 201 145
129 110 94 92 37 6
BbeI G_GCGC'C
Cuts at: 659 690 3061 3309 659
Size: 31 2371 248 2977
Fragments arranged by size:
2977 2371 248 31
BbsI GAAGACnn'nnnn_
Cuts at: 1148 1178 2241 1148
Size: 30 1063 4534
Fragments arranged by size:
4534 1063 30
BbvCI CC'TCA_GC
Cuts at: 1582 1582
Size: 5627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2184 2218 2184
Size: 34 5593
BciVI GTATCCnnnnn_n'
Cuts at: 3525 4286 5627 3525
Size: 761 1341 3525
Fragments arranged by size:
3525 1341 761
BclI T'GATC_A
Cuts at: 3147 3147
Size: 5627
BglI GCCn_nnn'nGGC
Cuts at: 761 848 887 1022 1367 1675 1801 1977 761
Size: 87 39 135 345 308 126 176 4411
Fragments arranged by size:
4411 345 308 176 135 126 87 39
BglII A'GATC_T
Cuts at: 3142 5179 3142
Size: 2037 3590
BlpI GC'TnA_GC
Cuts at: 236 2138 2739 236
Size: 1902 601 3124
Fragments arranged by size:
3124 1902 601
Bme1580I G_kGCm'C
Cuts at: 378 415 838 1057 1133 1262 1467 2185 3252
Size: 37 423 219 76 129 205 718 1067
Cuts at: 3252 3345 4401 378
Size: 93 1056 1604
Fragments arranged by size:
1604 1067 1056 718 423 219 205 129
93 76 37
BmgBI CAC'GTC
Cuts at: 210 210
Size: 5627
BmrI ACTGGGnnnn_n'
Cuts at: 417 993 1263 2839 2920 3255 5399 417
Size: 576 270 1576 81 335 2144 645
Fragments arranged by size:
2144 1576 645 576 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 753 963 1680 1851 2523 3994 753
Size: 210 717 171 672 1471 2386
Fragments arranged by size:
2386 1471 717 672 210 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 935 1733 1848 1938 2583 279
Size: 656 798 115 90 645 3323
Fragments arranged by size:
3323 798 656 645 115 90
Bpu10I CC'TnA_GC
Cuts at: 1582 1941 2312 1582
Size: 359 371 4897
Fragments arranged by size:
4897 371 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2803 3682 4174 4472 1465
Size: 1338 879 492 298 2620
Fragments arranged by size:
2620 1338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1899 5331 1899
Size: 3432 2195
BsaAI yAC'GTr
Cuts at: 599 2145 2619 2980 599
Size: 1546 474 361 3246
Fragments arranged by size:
3246 1546 474 361
BsaBI GATnn'nnATC
Cuts at: 6 3141 5178 6
Size: 3135 2037 455
Fragments arranged by size:
3135 2037 455
BsaHI Gr'CG_yC
Cuts at: 308 656 687 1832 3058 3306 4008 5504 308
Size: 348 31 1145 1226 248 702 1496 431
Fragments arranged by size:
1496 1226 1145 702 431 348 248 31
BsaWI w'CCGG_w
Cuts at: 2105 2816 3337 4015 4289 4436 4847 2105
Size: 711 521 678 274 147 411 2885
Fragments arranged by size:
2885 711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 1496 1526 370
Size: 30 1096 30 4471
Fragments arranged by size:
4471 1096 30 30
BseYI C'CCAG_C
Cuts at: 132 497 1125 1261 1281 1460 1673 2404 2463
Size: 365 628 136 20 179 213 731 59
Cuts at: 2463 3052 3810 4387 4883 132
Size: 589 758 577 496 876
Fragments arranged by size:
876 758 731 628 589 577 496 365
213 179 136 59 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 922
Size: 5627
BsiEI CG_ry'CG
Cuts at: 104 495 560 744 760 3169 3215 4423 104
Size: 391 65 184 16 2409 46 1208 1308
Fragments arranged by size:
2409 1308 1208 391 184 65 46 16
BsiHKAI G_wGCw'C
Cuts at: 838 949 1952 2144 2179 2641 3419 3609 4401
Size: 111 1003 192 35 462 778 190 792
Cuts at: 4401 838
Size: 2064
Fragments arranged by size:
2064 1003 792 778 462 192 190 111
35
BsmI GAATG_Cn'
Cuts at: 283 784 1327 283
Size: 501 543 4583
Fragments arranged by size:
4583 543 501
Bsp1286I G_dGCh'C
Cuts at: 378 415 507 838 949 1057 1133 1262 1467
Size: 37 92 331 111 108 76 129 205
Cuts at: 1467 1561 1952 2144 2179 2185 2386 2496 2641
Size: 94 391 192 35 6 201 110 145
Cuts at: 2641 3252 3345 3419 3609 3671 4401 378
Size: 611 93 74 190 62 730 1604
Fragments arranged by size:
1604 730 611 391 331 205 201 192
190 145 129 111 110 108 94 93
92 76 74 62 37 35 6
BspEI T'CCGG_A
Cuts at: 4847 4847
Size: 5627
BspHI T'CATG_A
Cuts at: 4803 4803
Size: 5627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1195 1521 1798 2677 2891 3193 3574 847
Size: 348 326 277 879 214 302 381 2900
Fragments arranged by size:
2900 879 381 348 326 302 277 214
BsrBI CCG'CTC
Cuts at: 50 1962 2243 3919 3973 5457 5622 50
Size: 1912 281 1676 54 1484 165 55
Fragments arranged by size:
1912 1676 1484 281 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3539 3539
Size: 5627
BsrFI r'CCGG_y
Cuts at: 559 755 1246 1285 1669 2105 3625 3806 4015
Size: 196 491 39 384 436 1520 181 209
Cuts at: 4015 559
Size: 2171
Fragments arranged by size:
2171 1520 491 436 384 209 196 181
39
BssHII G'CGCG_C
Cuts at: 634 862 634
Size: 228 5399
BssSI C'ACGA_G
Cuts at: 3898 4256 3898
Size: 358 5269
BstAPI GCAn_nnn'nTGC
Cuts at: 3004 3004
Size: 5627
BstBI TT'CG_AA
Cuts at: 110 3988 110
Size: 3878 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 5627
BstYI r'GATC_y
Cuts at: 1 2224 2532 2652 3119 3142 3477 3723 4735
Size: 2223 308 120 467 23 335 246 1012
Cuts at: 4735 4821 4833 5179 1
Size: 86 12 346 449
Fragments arranged by size:
2223 1012 467 449 346 335 308 246
120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1826 2006 1826
Size: 180 5447
BtgI C'CryG_G
Cuts at: 125 467 537 626 650 714 855 894 1293
Size: 342 70 89 24 64 141 39 399
Cuts at: 1293 2220 125
Size: 927 3532
Fragments arranged by size:
3532 927 399 342 141 89 70 64
39 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3577 3758 3921 3577
Size: 181 163 5283
Fragments arranged by size:
5283 181 163
BtsI GCAGTG_nn'
Cuts at: 250 378 570 796 910 1057 1059 1248 1385
Size: 128 192 226 114 147 2 189 137
Cuts at: 1385 2870 250
Size: 1485 3007
Fragments arranged by size:
3007 1485 226 192 189 147 137 128
114 2
DraI TTT'AAA
Cuts at: 2626 2626
Size: 5627
DraIII CAC_nnn'GTG
Cuts at: 900 900
Size: 5627
DrdI GACnn_nn'nnGTC
Cuts at: 180 1630 3333 4191 180
Size: 1450 1703 858 1616
Fragments arranged by size:
1703 1616 1450 858
EaeI y'GGCC_r
Cuts at: 218 450 741 757 921 1095 1885 2100 2376
Size: 232 291 16 164 174 790 215 276
Cuts at: 2376 3212 3386 3777 3804 5149 218
Size: 836 174 391 27 1345 696
Fragments arranged by size:
1345 836 790 696 391 291 276 232
215 174 174 164 27 16
EagI C'GGCC_G
Cuts at: 741 757 3212 741
Size: 16 2455 3156
Fragments arranged by size:
3156 2455 16
EarI CTCTTCn'nnn_
Cuts at: 1432 2018 2546 3650 3860 1432
Size: 586 528 1104 210 3199
Fragments arranged by size:
3199 1104 586 528 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2299 3835 4145 4291 5437 2242
Size: 57 1536 310 146 1146 2432
Fragments arranged by size:
2432 1536 1146 310 146 57
EcoICRI GAG'CTC
Cuts at: 1950 2177 2639 1950
Size: 227 462 4938
Fragments arranged by size:
4938 462 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 935 1733 1848 1938 2570 2583 3452 3884
Size: 656 798 115 90 632 13 869 432
Cuts at: 3884 4631 4899 279
Size: 747 268 1007
Fragments arranged by size:
1007 869 798 747 656 632 432 268
115 90 13
EcoO109I rG'GnC_Cy
Cuts at: 374 411 412 772 995 1129 1208 1258 1355
Size: 37 1 360 223 134 79 50 97
Cuts at: 1355 1464 1632 2002 2182 2233 2328 2425 2766
Size: 109 168 370 180 51 95 97 341
Cuts at: 2766 374
Size: 3235
Fragments arranged by size:
3235 370 360 341 223 180 168 134
109 97 97 95 79 51 50 37
1
EcoRI G'AATT_C
Cuts at: 2631 2631
Size: 5627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 3646 2425
Size: 1221 4406
FspI TGC'GCA
Cuts at: 252 909 3408 252
Size: 657 2499 2471
Fragments arranged by size:
2499 2471 657
FspAI rTGC'GCAy
Cuts at: 909 909
Size: 5627
HaeII r_GCGC'y
Cuts at: 546 659 690 821 1110 3061 3309 4331 5310
Size: 113 31 131 289 1951 248 1022 979
Cuts at: 5310 546
Size: 863
Fragments arranged by size:
1951 1022 979 863 289 248 131 113
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1165 1197 1463 1495 1678 1693 1710 1725 3469
Size: 32 266 32 183 15 17 15 1744
Cuts at: 3469 3499 3501 3531 1165
Size: 30 2 30 3261
Fragments arranged by size:
3261 1744 266 183 32 32 30 30
17 15 15 2
HincII GTy'rAC
Cuts at: 2666 2666
Size: 5627
KasI G'GCGC_C
Cuts at: 655 686 3057 3305 655
Size: 31 2371 248 2977
Fragments arranged by size:
2977 2371 248 31
KpnI G_GTAC'C
Cuts at: 1163 1913 2647 1163
Size: 750 734 4143
Fragments arranged by size:
4143 750 734
MluI A'CGCG_T
Cuts at: 5060 5060
Size: 5627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4298 4482 4298
Size: 184 5443
MscI TGG'CCA
Cuts at: 220 1097 2102 3388 220
Size: 877 1005 1286 2459
Fragments arranged by size:
2459 1286 1005 877
MslI CAynn'nnrTG
Cuts at: 212 917 3616 3743 212
Size: 705 2699 127 2096
Fragments arranged by size:
2699 2096 705 127
MspA1I CmG'CkG
Cuts at: 150 392 497 509 628 692 704 746 812
Size: 242 105 12 119 64 12 42 66
Cuts at: 812 857 917 926 965 1265 2222 2738 3052
Size: 45 60 9 39 300 957 516 314
Cuts at: 3052 3412 4425 4670 150
Size: 360 1013 245 1107
Fragments arranged by size:
1107 1013 957 516 360 314 300 245
242 119 105 66 64 60 45 42
39 12 12 9
NaeI GCC'GGC
Cuts at: 757 1248 1671 3808 757
Size: 491 423 2137 2576
Fragments arranged by size:
2576 2137 491 423
NarI GG'CG_CC
Cuts at: 656 687 3058 3306 656
Size: 31 2371 248 2977
Fragments arranged by size:
2977 2371 248 31
NcoI C'CATG_G
Cuts at: 125 1293 125
Size: 1168 4459
NgoMIV G'CCGG_C
Cuts at: 755 1246 1669 3806 755
Size: 491 423 2137 2576
Fragments arranged by size:
2576 2137 491 423
NspI r_CATG'y
Cuts at: 2205 2680 3180 4087 2205
Size: 475 500 907 3745
Fragments arranged by size:
3745 907 500 475
PasI CC'CwG_GG
Cuts at: 1601 1601
Size: 5627
PciI A'CATG_T
Cuts at: 4083 4083
Size: 5627
PflMI CCAn_nnn'nTGG
Cuts at: 1093 1372 2404 2509 1093
Size: 279 1032 105 4211
Fragments arranged by size:
4211 1032 279 105
PfoI T'CCnGG_A
Cuts at: 1516 2168 1516
Size: 652 4975
PmeI GTTT'AAAC
Cuts at: 2626 2626
Size: 5627
PmlI CAC'GTG
Cuts at: 599 2145 599
Size: 1546 4081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 4766
Size: 32 5595
PpuMI rG'GwC_Cy
Cuts at: 772 995 1208 1355 1632 2002 772
Size: 223 213 147 277 370 4397
Fragments arranged by size:
4397 370 277 223 213 147
PspOMI G'GGCC_C
Cuts at: 374 411 1129 1258 1463 2181 374
Size: 37 718 129 205 718 3820
Fragments arranged by size:
3820 718 718 205 129 37
PstI C_TGCA'G
Cuts at: 245 385 697 817 1243 1532 1809 2674 3359
Size: 140 312 120 426 289 277 865 685
Cuts at: 3359 245
Size: 2513
Fragments arranged by size:
2513 865 685 426 312 289 277 140
120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5627
PvuII CAG'CTG
Cuts at: 917 965 1265 3052 3412 917
Size: 48 300 1787 360 3132
Fragments arranged by size:
3132 1787 360 300 48
RsrII CG'GwC_CG
Cuts at: 2454 3822 2454
Size: 1368 4259
SacI G_AGCT'C
Cuts at: 1952 2179 2641 1952
Size: 227 462 4938
Fragments arranged by size:
4938 462 227
SacII CC_GC'GG
Cuts at: 629 858 2223 629
Size: 229 1365 4033
Fragments arranged by size:
4033 1365 229
SalI G'TCGA_C
Cuts at: 2664 2664
Size: 5627
SanDI GG'GwC_CC
Cuts at: 772 995 772
Size: 223 5404
SapI GCTCTTCn'nnn_
Cuts at: 1432 3650 3860 1432
Size: 2218 210 3199
Fragments arranged by size:
3199 2218 210
SbfI CC_TGCA'GG
Cuts at: 2674 2674
Size: 5627
ScaI AGT'ACT
Cuts at: 406 5144 406
Size: 4738 889
SfcI C'TryA_G
Cuts at: 33 241 381 693 813 1239 1528 1805 2670
Size: 208 140 312 120 426 289 277 865
Cuts at: 2670 3355 4348 4539 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 865 685 426 312 289 277
208 191 140 120
SfoI GGC'GCC
Cuts at: 657 688 3059 3307 657
Size: 31 2371 248 2977
Fragments arranged by size:
2977 2371 248 31
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5627
SgrAI Cr'CCGG_yG
Cuts at: 2105 2105
Size: 5627
SmaI CCC'GGG
Cuts at: 398 1987 2085 2649 398
Size: 1589 98 564 3376
Fragments arranged by size:
3376 1589 564 98
SmlI C'TyrA_G
Cuts at: 1480 2782 3697 4189 4451 1480
Size: 1302 915 492 262 2656
Fragments arranged by size:
2656 1302 915 492 262
SnaBI TAC'GTA
Cuts at: 2619 2619
Size: 5627
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5627
SphI G_CATG'C
Cuts at: 2680 3180 2680
Size: 500 5127
SrfI GCCC'GGGC
Cuts at: 398 398
Size: 5627
StuI AGG'CCT
Cuts at: 1078 1078
Size: 5627
StyI C'CwwG_G
Cuts at: 125 1293 2291 2761 3115 125
Size: 1168 998 470 354 2637
Fragments arranged by size:
2637 1168 998 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1881 3210 3343 3802 4011 1593
Size: 288 1329 133 459 209 3209
Fragments arranged by size:
3209 1329 459 288 209 133
TatI w'GTAC_w
Cuts at: 404 5142 404
Size: 4738 889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 1496 1528 5144 5176 370
Size: 32 1094 32 3616 32 821
Fragments arranged by size:
3616 1094 821 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1234 3424 1234
Size: 2190 3437
XbaI T'CTAG_A
Cuts at: 2658 2658
Size: 5627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1348 1369 2427 3122 1348
Size: 21 1058 695 3853
Fragments arranged by size:
3853 1058 695 21
XmaI C'CCGG_G
Cuts at: 396 1985 2083 2647 396
Size: 1589 98 564 3376
Fragments arranged by size:
3376 1589 564 98
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BseRI BsiWI BsmBI BsrGI BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SexAI SfiI SspI SwaI XhoI XmnI ZraI