Restriction map for
pF1KSDA0150
(Circular) (Six-base) MAPSORT of: pF1KSDA0150.seq Check: 6469 from: 1 to: 5981
pF1KSDA0150 5981 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1530 2156 2254 1530
Size: 626 98 5257
Fragments arranged by size:
5257 626 98
AccI GT'mk_AC
Cuts at: 3019 3019
Size: 5981
Acc65I G'GTAC_C
Cuts at: 220 2997 220
Size: 2777 3204
AclI AA'CG_TT
Cuts at: 5821 5821
Size: 5981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 2344 2609 3806 4238 4985 5253 1615
Size: 729 265 1197 432 747 268 2343
Fragments arranged by size:
2343 1197 747 729 432 268 265
AfeI AGC'GCT
Cuts at: 2185 2185
Size: 5981
AflIII A'CryG_T
Cuts at: 2363 2369 3333 4437 5414 2363
Size: 6 964 1104 977 2930
Fragments arranged by size:
2930 1104 977 964 6
AgeI A'CCGG_T
Cuts at: 4369 4369
Size: 5981
AleI CACnn'nnGTG
Cuts at: 844 844
Size: 5981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2844 2844
Size: 5981
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 852 884 852
Size: 32 5949
AlwNI CAG_nnn'CTG
Cuts at: 174 235 1240 2348 2843 3286 4853 174
Size: 61 1005 1108 495 443 1567 1302
Fragments arranged by size:
1567 1302 1108 1005 495 443 61
ApaI G_GGCC'C
Cuts at: 420 549 970 1107 1668 1821 2570 2615 2913
Size: 129 421 137 561 153 749 45 298
Cuts at: 2913 2922 420
Size: 9 3479
Fragments arranged by size:
3479 749 561 421 298 153 137 129
45 9
ApaLI G'TGCA_C
Cuts at: 2223 4751 2223
Size: 2528 3453
ApoI r'AATT_y
Cuts at: 568 2985 5334 5590 568
Size: 2417 2349 256 959
Fragments arranged by size:
2417 2349 959 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
AvaI C'yCGr_G
Cuts at: 366 419 602 626 645 761 1001 1088 2206
Size: 53 183 24 19 116 240 87 1118
Cuts at: 2206 3001 366
Size: 795 3346
Fragments arranged by size:
3346 1118 795 240 183 116 87 53
24 19
AvrII C'CTAG_G
Cuts at: 2914 2914
Size: 5981
BamHI G'GATC_C
Cuts at: 2037 3006 2037
Size: 969 5012
BanI G'GyrC_C
Cuts at: 220 1513 2242 2494 2793 2997 3411 3659 3694
Size: 1293 729 252 299 204 414 248 35
Cuts at: 3694 220
Size: 2507
Fragments arranged by size:
2507 1293 729 414 299 252 248 204
35
BanII G_rGCy'C
Cuts at: 420 549 559 623 770 970 1107 1202 1668
Size: 129 10 64 147 200 137 95 466
Cuts at: 1668 1821 1903 2570 2615 2887 2895 2913 2922
Size: 153 82 667 45 272 8 18 9
Cuts at: 2922 2995 4025 420
Size: 73 1030 2376
Fragments arranged by size:
2376 1030 667 466 272 200 153 147
137 129 95 82 73 64 45 18
10 9 8
BbeI G_GCGC'C
Cuts at: 2498 3415 3663 2498
Size: 917 248 4816
Fragments arranged by size:
4816 917 248
BbsI GAAGACnn'nnnn_
Cuts at: 1606 1765 1606
Size: 159 5822
BbvCI CC'TCA_GC
Cuts at: 1546 1546
Size: 5981
BciVI GTATCCnnnnn_n'
Cuts at: 3879 4640 5981 3879
Size: 761 1341 3879
Fragments arranged by size:
3879 1341 761
BclI T'GATC_A
Cuts at: 1730 3501 1730
Size: 1771 4210
BglI GCCn_nnn'nGGC
Cuts at: 305 333 544 648 805 979 1006 1812 2074
Size: 28 211 104 157 174 27 806 262
Cuts at: 2074 2917 305
Size: 843 3369
Fragments arranged by size:
3369 843 806 262 211 174 157 104
28 27
BglII A'GATC_T
Cuts at: 3496 5533 3496
Size: 2037 3944
BlpI GC'TnA_GC
Cuts at: 3093 3093
Size: 5981
Bme1580I G_kGCm'C
Cuts at: 282 420 549 970 1107 1668 1821 2227 2245
Size: 138 129 421 137 561 153 406 18
Cuts at: 2245 2461 2570 2615 2913 2922 3606 3699 4755
Size: 216 109 45 298 9 684 93 1056
Cuts at: 4755 282
Size: 1508
Fragments arranged by size:
1508 1056 684 561 421 406 298 216
153 138 137 129 109 93 45 18
9
BmgBI CAC'GTC
Cuts at: 1223 2552 2965 1223
Size: 1329 413 4239
Fragments arranged by size:
4239 1329 413
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1988 2493 3193 3274 3609 5753 1345
Size: 643 505 700 81 335 2144 1573
Fragments arranged by size:
2144 1573 700 643 505 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1963 4348 1963
Size: 2385 3596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 1254 1517 2788 606
Size: 648 263 1271 3799
Fragments arranged by size:
3799 1271 648 263
Bpu10I CC'TnA_GC
Cuts at: 1546 2817 1546
Size: 1271 4710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 3157 4036 4528 4826 478
Size: 2679 879 492 298 1633
Fragments arranged by size:
2679 1633 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1417 2601 5685 1417
Size: 1184 3084 1713
Fragments arranged by size:
3084 1713 1184
BsaAI yAC'GTr
Cuts at: 2364 2370 2973 3334 2364
Size: 6 603 361 5011
Fragments arranged by size:
5011 603 361 6
BsaBI GATnn'nnATC
Cuts at: 6 3495 5532 6
Size: 3489 2037 455
Fragments arranged by size:
3489 2037 455
BsaHI Gr'CG_yC
Cuts at: 1762 2495 2653 3412 3660 4362 5858 1762
Size: 733 158 759 248 702 1496 1885
Fragments arranged by size:
1885 1496 759 733 702 248 158
BsaWI w'CCGG_w
Cuts at: 1043 1469 3170 3691 4369 4643 4790 5201 1043
Size: 426 1701 521 678 274 147 411 1823
Fragments arranged by size:
1823 1701 678 521 426 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1321 1351 1957 1987 2786 2816 1321
Size: 30 606 30 799 30 4486
Fragments arranged by size:
4486 799 606 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1065 1275 1278 2736 2739 2742 2745 2748 2751
Size: 210 3 1458 3 3 3 3 3
Cuts at: 2751 1065
Size: 4295
Fragments arranged by size:
4295 1458 210 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 225 548 864 1246 1340 2569 2633 2656 2894
Size: 323 316 382 94 1229 64 23 238
Cuts at: 2894 3406 4164 4741 5237 225
Size: 512 758 577 496 969
Fragments arranged by size:
1229 969 758 577 512 496 382 323
316 238 94 64 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2101 2675 2935 1697
Size: 404 574 260 4743
Fragments arranged by size:
4743 574 404 260
BsiEI CG_ry'CG
Cuts at: 104 406 3523 3569 4777 104
Size: 302 3117 46 1208 1308
Fragments arranged by size:
3117 1308 1208 302 46
BsiHKAI G_wGCw'C
Cuts at: 894 2227 2314 2833 2995 3773 3963 4755 894
Size: 1333 87 519 162 778 190 792 2120
Fragments arranged by size:
2120 1333 792 778 519 190 162 87
BsmBI CGTCTCn'nnnn_
Cuts at: 1467 1467
Size: 5981
Bsp1286I G_dGCh'C
Cuts at: 282 420 549 559 623 770 894 970 1107
Size: 138 129 10 64 147 124 76 137
Cuts at: 1107 1202 1668 1821 1903 2227 2245 2314 2461
Size: 95 466 153 82 324 18 69 147
Cuts at: 2461 2570 2615 2833 2887 2895 2913 2922 2995
Size: 109 45 218 54 8 18 9 73
Cuts at: 2995 3606 3699 3773 3963 4025 4755 282
Size: 611 93 74 190 62 730 1508
Fragments arranged by size:
1508 730 611 466 324 218 190 153
147 147 138 137 129 124 109 95
93 82 76 74 73 69 64 62
54 45 18 18 10 9 8
BspEI T'CCGG_A
Cuts at: 1043 1469 5201 1043
Size: 426 3732 1823
Fragments arranged by size:
3732 1823 426
BspHI T'CATG_A
Cuts at: 5157 5157
Size: 5981
BspMI ACCTGCnnnn'nnnn_
Cuts at: 679 1530 2156 2254 2275 3031 3245 3547 3928
Size: 851 626 98 21 756 214 302 381
Cuts at: 3928 679
Size: 2732
Fragments arranged by size:
2732 851 756 626 381 302 214 98
21
BsrBI CCG'CTC
Cuts at: 50 826 1431 4273 4327 5811 5976 50
Size: 776 605 2842 54 1484 165 55
Fragments arranged by size:
2842 1484 776 605 165 55 54
BsrDI GCAATG_nn'
Cuts at: 210 3893 210
Size: 3683 2298
BsrFI r'CCGG_y
Cuts at: 978 1318 1764 2030 2304 3979 4160 4369 978
Size: 340 446 266 274 1675 181 209 2590
Fragments arranged by size:
2590 1675 446 340 274 266 209 181
BssSI C'ACGA_G
Cuts at: 352 2788 4252 4610 352
Size: 2436 1464 358 1723
Fragments arranged by size:
2436 1723 1464 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1704 3358 1704
Size: 1654 4327
BstBI TT'CG_AA
Cuts at: 110 4342 110
Size: 4232 1749
BstEII G'GTnAC_C
Cuts at: 1626 1626
Size: 5981
BstXI CCAn_nnnn'nTGG
Cuts at: 1845 1845
Size: 5981
BstYI r'GATC_y
Cuts at: 1 155 684 2037 3006 3473 3496 3831 4077
Size: 154 529 1353 969 467 23 335 246
Cuts at: 4077 5089 5175 5187 5533 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1353 1012 969 529 467 449 346 335
246 154 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 540 984 1570 540
Size: 444 586 4951
Fragments arranged by size:
4951 586 444
BtgI C'CryG_G
Cuts at: 125 198 321 878 2175 2490 2607 2684 125
Size: 73 123 557 1297 315 117 77 3422
Fragments arranged by size:
3422 1297 557 315 123 117 77 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3931 4112 4275 3931
Size: 181 163 5637
Fragments arranged by size:
5637 181 163
BtsI GCAGTG_nn'
Cuts at: 681 750 2084 2672 2700 3224 681
Size: 69 1334 588 28 524 3438
Fragments arranged by size:
3438 1334 588 524 69 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1148 1183 1148
Size: 35 5946
DraI TTT'AAA
Cuts at: 2980 2980
Size: 5981
DraIII CAC_nnn'GTG
Cuts at: 2286 2286
Size: 5981
DrdI GACnn_nn'nnGTC
Cuts at: 3687 4545 3687
Size: 858 5123
EaeI y'GGCC_r
Cuts at: 403 881 927 1986 2066 2093 2216 2600 3566
Size: 478 46 1059 80 27 123 384 966
Cuts at: 3566 3740 4131 4158 5503 403
Size: 174 391 27 1345 881
Fragments arranged by size:
1345 1059 966 881 478 391 384 174
123 80 46 27 27
EagI C'GGCC_G
Cuts at: 403 3566 403
Size: 3163 2818
EarI CTCTTCn'nnn_
Cuts at: 318 695 1075 1296 1444 1573 2122 4004 4214
Size: 377 380 221 148 129 549 1882 210
Cuts at: 4214 318
Size: 2085
Fragments arranged by size:
2085 1882 549 380 377 221 210 148
129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 1572 1929 4189 4499 4645 5791 148
Size: 1424 357 2260 310 146 1146 338
Fragments arranged by size:
2260 1424 1146 357 338 310 146
EcoICRI GAG'CTC
Cuts at: 2993 2993
Size: 5981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 1254 1517 1615 2344 2609 2788 3806 4238
Size: 648 263 98 729 265 179 1018 432
Cuts at: 4238 4985 5253 606
Size: 747 268 1334
Fragments arranged by size:
1334 1018 747 729 648 432 268 265
263 179 98
EcoNI CCTnn'n_nnAGG
Cuts at: 166 1621 2929 166
Size: 1455 1308 3218
Fragments arranged by size:
3218 1455 1308
EcoO109I rG'GnC_Cy
Cuts at: 370 416 545 599 668 966 967 988 1103
Size: 46 129 54 69 298 1 21 115
Cuts at: 1103 1104 1364 1566 1614 1841 1944 2012 2264
Size: 1 260 202 48 227 103 68 252
Cuts at: 2264 2566 2910 2918 2919 3120 370
Size: 302 344 8 1 201 3231
Fragments arranged by size:
3231 344 302 298 260 252 227 202
201 129 115 103 69 68 54 48
46 21 8 1 1 1
EcoRI G'AATT_C
Cuts at: 2985 2985
Size: 5981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 706 828 4000 706
Size: 122 3172 2687
Fragments arranged by size:
3172 2687 122
FspI TGC'GCA
Cuts at: 2023 3762 2023
Size: 1739 4242
HaeII r_GCGC'y
Cuts at: 1784 2187 2498 2640 3415 3663 4685 5664 1784
Size: 403 311 142 775 248 1022 979 2101
Fragments arranged by size:
2101 1022 979 775 403 311 248 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1321 1353 1553 1585 2782 2814 3823 3853 3855
Size: 32 200 32 1197 32 1009 30 2
Cuts at: 3855 3885 1321
Size: 30 3417
Fragments arranged by size:
3417 1197 1009 200 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3020 3020
Size: 5981
KasI G'GCGC_C
Cuts at: 2494 3411 3659 2494
Size: 917 248 4816
Fragments arranged by size:
4816 917 248
KpnI G_GTAC'C
Cuts at: 224 3001 224
Size: 2777 3204
MluI A'CGCG_T
Cuts at: 5414 5414
Size: 5981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1292 2858 4652 4836 1292
Size: 1566 1794 184 2437
Fragments arranged by size:
2437 1794 1566 184
MscI TGG'CCA
Cuts at: 883 929 2068 2095 3742 883
Size: 46 1139 27 1647 3122
Fragments arranged by size:
3122 1647 1139 46 27
MslI CAynn'nnrTG
Cuts at: 203 844 3970 4097 203
Size: 641 3126 127 2087
Fragments arranged by size:
3126 2087 641 127
MspA1I CmG'CkG
Cuts at: 552 636 887 1877 1931 2051 2492 2508 3092
Size: 84 251 990 54 120 441 16 584
Cuts at: 3092 3406 3766 4779 5024 552
Size: 314 360 1013 245 1509
Fragments arranged by size:
1509 1013 990 584 441 360 314 251
245 120 84 54 16
NaeI GCC'GGC
Cuts at: 1320 1766 2032 4162 1320
Size: 446 266 2130 3139
Fragments arranged by size:
3139 2130 446 266
NarI GG'CG_CC
Cuts at: 2495 3412 3660 2495
Size: 917 248 4816
Fragments arranged by size:
4816 917 248
NcoI C'CATG_G
Cuts at: 125 321 125
Size: 196 5785
NgoMIV G'CCGG_C
Cuts at: 1318 1764 2030 4160 1318
Size: 446 266 2130 3139
Fragments arranged by size:
3139 2130 446 266
NspI r_CATG'y
Cuts at: 454 1702 2148 3034 3534 4441 454
Size: 1248 446 886 500 907 1994
Fragments arranged by size:
1994 1248 907 886 500 446
PasI CC'CwG_GG
Cuts at: 852 2421 2563 2707 2900 852
Size: 1569 142 144 193 3933
Fragments arranged by size:
3933 1569 193 144 142
PciI A'CATG_T
Cuts at: 4437 4437
Size: 5981
PflMI CCAn_nnn'nTGG
Cuts at: 841 841
Size: 5981
PfoI T'CCnGG_A
Cuts at: 1494 2804 1494
Size: 1310 4671
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5981
PmlI CAC'GTG
Cuts at: 2364 2364
Size: 5981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 5120
Size: 32 5949
PpuMI rG'GwC_Cy
Cuts at: 370 668 988 1364 1841 1944 2264 370
Size: 298 320 376 477 103 320 4087
Fragments arranged by size:
4087 477 376 320 320 298 103
PspOMI G'GGCC_C
Cuts at: 416 545 966 1103 1664 1817 2566 2611 2909
Size: 129 421 137 561 153 749 45 298
Cuts at: 2909 2918 416
Size: 9 3479
Fragments arranged by size:
3479 749 561 421 298 153 137 129
45 9
PspXI vC'TCGA_Gb
Cuts at: 645 645
Size: 5981
PstI C_TGCA'G
Cuts at: 170 641 676 1128 2046 2338 2395 2873 3028
Size: 471 35 452 918 292 57 478 155
Cuts at: 3028 3713 170
Size: 685 2438
Fragments arranged by size:
2438 918 685 478 471 452 292 155
57 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5981
PvuII CAG'CTG
Cuts at: 636 887 2051 2508 3406 3766 636
Size: 251 1164 457 898 360 2851
Fragments arranged by size:
2851 1164 898 457 360 251
RsrII CG'GwC_CG
Cuts at: 4176 4176
Size: 5981
SacI G_AGCT'C
Cuts at: 2995 2995
Size: 5981
SacII CC_GC'GG
Cuts at: 2493 2493
Size: 5981
SalI G'TCGA_C
Cuts at: 3018 3018
Size: 5981
SanDI GG'GwC_CC
Cuts at: 370 370
Size: 5981
SapI GCTCTTCn'nnn_
Cuts at: 1444 1573 4004 4214 1444
Size: 129 2431 210 3211
Fragments arranged by size:
3211 2431 210 129
SbfI CC_TGCA'GG
Cuts at: 2046 3028 2046
Size: 982 4999
ScaI AGT'ACT
Cuts at: 2140 5498 2140
Size: 3358 2623
SexAI A'CCwGG_T
Cuts at: 2227 2227
Size: 5981
SfcI C'TryA_G
Cuts at: 33 166 637 672 1124 2042 2052 2334 2355
Size: 133 471 35 452 918 10 282 21
Cuts at: 2355 2391 2869 3024 3709 4702 4893 33
Size: 36 478 155 685 993 191 1121
Fragments arranged by size:
1121 993 918 685 478 471 452 282
191 155 133 36 35 21 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 1006 2917 1006
Size: 1911 4070
SfoI GGC'GCC
Cuts at: 2496 3413 3661 2496
Size: 917 248 4816
Fragments arranged by size:
4816 917 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
SmaI CCC'GGG
Cuts at: 368 421 604 763 1003 1090 3003 368
Size: 53 183 159 240 87 1913 3346
Fragments arranged by size:
3346 1913 240 183 159 87 53
SmlI C'TyrA_G
Cuts at: 457 645 3136 4051 4543 4805 457
Size: 188 2491 915 492 262 1633
Fragments arranged by size:
2491 1633 915 492 262 188
SnaBI TAC'GTA
Cuts at: 2973 2973
Size: 5981
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5981
SphI G_CATG'C
Cuts at: 1702 3034 3534 1702
Size: 1332 500 4149
Fragments arranged by size:
4149 1332 500
StuI AGG'CCT
Cuts at: 1009 1144 1267 1009
Size: 135 123 5723
Fragments arranged by size:
5723 135 123
StyI C'CwwG_G
Cuts at: 125 321 903 1679 1906 2291 2780 2914 2930
Size: 196 582 776 227 385 489 134 16
Cuts at: 2930 3115 3469 125
Size: 185 354 2637
Fragments arranged by size:
2637 776 582 489 385 354 227 196
185 134 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 227 264 1053 1731 2413 3564 3697 4156 4365
Size: 37 789 678 682 1151 133 459 209
Cuts at: 4365 227
Size: 1843
Fragments arranged by size:
1843 1151 789 682 678 459 209 133
37
TatI w'GTAC_w
Cuts at: 1253 2138 2147 5496 1253
Size: 885 9 3349 1738
Fragments arranged by size:
3349 1738 885 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 660 692 768 800 1476 1508 2272 2304 2786
Size: 32 76 32 676 32 764 32 482
Cuts at: 2786 2818 5498 5530 660
Size: 32 2680 32 1111
Fragments arranged by size:
2680 1111 764 676 482 76 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1643 3778 1643
Size: 2135 3846
XbaI T'CTAG_A
Cuts at: 3012 3012
Size: 5981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 709 1614 3476 709
Size: 905 1862 3214
Fragments arranged by size:
3214 1862 905
XhoI C'TCGA_G
Cuts at: 645 645
Size: 5981
XmaI C'CCGG_G
Cuts at: 366 419 602 761 1001 1088 3001 366
Size: 53 183 159 240 87 1913 3346
Fragments arranged by size:
3346 1913 240 183 159 87 53
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI BaeI BcgI BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstZ17I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PsrI SgrAI SrfI SspI
SwaI XmnI ZraI