Restriction map for
pF1KSDA0153
(Circular) (Six-base) MAPSORT of: pF1KSDA0153.seq Check: 8713 from: 1 to: 5069
pF1KSDA0153 5069 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 774 1053 1249 1689 525
Size: 249 279 196 440 3905
Fragments arranged by size:
3905 440 279 249 196
AatII G_ACGT'C
Cuts at: 1766 2003 1766
Size: 237 4832
AccI GT'mk_AC
Cuts at: 1046 1874 2057 2107 1046
Size: 828 183 50 4008
Fragments arranged by size:
4008 828 183 50
Acc65I G'GTAC_C
Cuts at: 1976 2085 1976
Size: 109 4960
AclI AA'CG_TT
Cuts at: 264 4909 264
Size: 4645 424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1714 1906 1989 2894 3326 4073 4341 1714
Size: 192 83 905 432 747 268 2442
Fragments arranged by size:
2442 905 747 432 268 192 83
AflIII A'CryG_T
Cuts at: 2421 3525 4502 2421
Size: 1104 977 2988
Fragments arranged by size:
2988 1104 977
AgeI A'CCGG_T
Cuts at: 3457 3457
Size: 5069
AleI CACnn'nnGTG
Cuts at: 1054 1054
Size: 5069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 910 1330 910
Size: 420 4649
AlwNI CAG_nnn'CTG
Cuts at: 528 610 1935 2374 3941 528
Size: 82 1325 439 1567 1656
Fragments arranged by size:
1656 1567 1325 439 82
ApaI G_GGCC'C
Cuts at: 906 1276 906
Size: 370 4699
ApaLI G'TGCA_C
Cuts at: 1018 1702 3839 1018
Size: 684 2137 2248
Fragments arranged by size:
2248 2137 684
ApoI r'AATT_y
Cuts at: 1747 2073 4422 4678 1747
Size: 326 2349 256 2138
Fragments arranged by size:
2349 2138 326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
AvaI C'yCGr_G
Cuts at: 145 174 374 402 541 842 940 1267 1723
Size: 29 200 28 139 301 98 327 456
Cuts at: 1723 1856 2089 145
Size: 133 233 3125
Fragments arranged by size:
3125 456 327 301 233 200 139 133
98 29 28
BamHI G'GATC_C
Cuts at: 725 2094 725
Size: 1369 3700
BanI G'GyrC_C
Cuts at: 537 759 1445 1976 2085 2499 2747 2782 537
Size: 222 686 531 109 414 248 35 2824
Fragments arranged by size:
2824 686 531 414 248 222 109 35
BanII G_rGCy'C
Cuts at: 452 906 1276 2083 3113 452
Size: 454 370 807 1030 2408
Fragments arranged by size:
2408 1030 807 454 370
BbeI G_GCGC'C
Cuts at: 2503 2751 2503
Size: 248 4821
BbsI GAAGACnn'nnnn_
Cuts at: 1517 1995 1517
Size: 478 4591
BbvCI CC'TCA_GC
Cuts at: 677 925 677
Size: 248 4821
BciVI GTATCCnnnnn_n'
Cuts at: 2967 3728 5069 2967
Size: 761 1341 2967
Fragments arranged by size:
2967 1341 761
BclI T'GATC_A
Cuts at: 2589 2589
Size: 5069
BglI GCCn_nnn'nGGC
Cuts at: 1271 1271
Size: 5069
BglII A'GATC_T
Cuts at: 1775 2584 4621 1775
Size: 809 2037 2223
Fragments arranged by size:
2223 2037 809
BlpI GC'TnA_GC
Cuts at: 2181 2181
Size: 5069
Bme1580I G_kGCm'C
Cuts at: 542 746 906 1022 1276 1706 2694 2787 3843
Size: 204 160 116 254 430 988 93 1056
Cuts at: 3843 542
Size: 1768
Fragments arranged by size:
1768 1056 988 430 254 204 160 116
93
BmgBI CAC'GTC
Cuts at: 500 741 1056 1416 1515 500
Size: 241 315 360 99 4054
Fragments arranged by size:
4054 360 315 241 99
BmrI ACTGGGnnnn_n'
Cuts at: 279 593 1314 2281 2362 2697 4841 279
Size: 314 721 967 81 335 2144 507
Fragments arranged by size:
2144 967 721 507 335 314 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 5069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 436 646 994 1957 313
Size: 123 210 348 963 3425
Fragments arranged by size:
3425 963 348 210 123
Bpu10I CC'TnA_GC
Cuts at: 677 925 677
Size: 248 4821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2245 3124 3616 3914 2245
Size: 879 492 298 3400
Fragments arranged by size:
3400 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4773 4773
Size: 5069
BsaAI yAC'GTr
Cuts at: 2061 2422 2061
Size: 361 4708
BsaBI GATnn'nnATC
Cuts at: 6 2583 4620 6
Size: 2577 2037 455
Fragments arranged by size:
2577 2037 455
BsaHI Gr'CG_yC
Cuts at: 184 1118 1763 2000 2500 2748 3450 4946 184
Size: 934 645 237 500 248 702 1496 307
Fragments arranged by size:
1496 934 702 645 500 307 248 237
BsaWI w'CCGG_w
Cuts at: 878 2258 2779 3457 3731 3878 4289 878
Size: 1380 521 678 274 147 411 1658
Fragments arranged by size:
1658 1380 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 619
Size: 30 5039
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 358 370 373 643 694 847 205
Size: 153 12 3 270 51 153 4427
Fragments arranged by size:
4427 270 153 153 51 12 3
BseYI C'CCAG_C
Cuts at: 313 745 2494 3252 3829 4325 313
Size: 432 1749 758 577 496 1057
Fragments arranged by size:
1749 1057 758 577 496 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 268 535 1078 1414 211
Size: 57 267 543 336 3866
Fragments arranged by size:
3866 543 336 267 57
BsiEI CG_ry'CG
Cuts at: 104 2611 2657 3865 104
Size: 2507 46 1208 1308
Fragments arranged by size:
2507 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 299 515 959 1022 1459 1706 2083 2861 3051
Size: 216 444 63 437 247 377 778 190
Cuts at: 3051 3843 299
Size: 792 1525
Fragments arranged by size:
1525 792 778 444 437 377 247 216
190 63
BsmBI CGTCTCn'nnnn_
Cuts at: 856 856
Size: 5069
Bsp1286I G_dGCh'C
Cuts at: 299 452 515 542 746 906 959 1022 1276
Size: 153 63 27 204 160 53 63 254
Cuts at: 1276 1459 1706 2083 2694 2787 2861 3051 3113
Size: 183 247 377 611 93 74 190 62
Cuts at: 3113 3843 299
Size: 730 1525
Fragments arranged by size:
1525 730 611 377 254 247 204 190
183 160 153 93 74 63 63 62
53 27
BspEI T'CCGG_A
Cuts at: 878 4289 878
Size: 3411 1658
BspHI T'CATG_A
Cuts at: 1667 4245 1667
Size: 2578 2491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 525 774 1053 1223 1249 1310 1689 1964 2056
Size: 249 279 170 26 61 379 275 92
Cuts at: 2056 2119 2333 2635 3016 525
Size: 63 214 302 381 2578
Fragments arranged by size:
2578 381 379 302 279 275 249 214
170 92 63 61 26
BsrBI CCG'CTC
Cuts at: 50 138 446 3361 3415 4899 5064 50
Size: 88 308 2915 54 1484 165 55
Fragments arranged by size:
2915 1484 308 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 2981 2981
Size: 5069
BsrFI r'CCGG_y
Cuts at: 152 690 3067 3248 3457 152
Size: 538 2377 181 209 1764
Fragments arranged by size:
2377 1764 538 209 181
BssHII G'CGCG_C
Cuts at: 197 243 517 1249 1396 197
Size: 46 274 732 147 3870
Fragments arranged by size:
3870 732 274 147 46
BssSI C'ACGA_G
Cuts at: 298 3340 3698 298
Size: 3042 358 1669
Fragments arranged by size:
3042 1669 358
BstAPI GCAn_nnn'nTGC
Cuts at: 537 2446 537
Size: 1909 3160
BstBI TT'CG_AA
Cuts at: 110 3430 110
Size: 3320 1749
BstEII G'GTnAC_C
Cuts at: 837 837
Size: 5069
BstXI CCAn_nnnn'nTGG
Cuts at: 570 2032 570
Size: 1462 3607
BstYI r'GATC_y
Cuts at: 1 725 1373 1775 1931 2094 2561 2584 2919
Size: 724 648 402 156 163 467 23 335
Cuts at: 2919 3165 4177 4263 4275 4621 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 724 648 467 449 402 346 335
246 163 156 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 811 811
Size: 5069
BtgI C'CryG_G
Cuts at: 125 585 666 755 1013 1026 125
Size: 460 81 89 258 13 4168
Fragments arranged by size:
4168 460 258 89 81 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1230 3019 3200 3363 1230
Size: 1789 181 163 2936
Fragments arranged by size:
2936 1789 181 163
BtsI GCAGTG_nn'
Cuts at: 2312 2312
Size: 5069
DraI TTT'AAA
Cuts at: 2068 2068
Size: 5069
DrdI GACnn_nn'nnGTC
Cuts at: 1414 2775 3633 1414
Size: 1361 858 2850
Fragments arranged by size:
2850 1361 858
EaeI y'GGCC_r
Cuts at: 209 560 1029 1067 2654 2828 3219 3246 4591
Size: 351 469 38 1587 174 391 27 1345
Cuts at: 4591 209
Size: 687
Fragments arranged by size:
1587 1345 687 469 391 351 174 38
27
EagI C'GGCC_G
Cuts at: 2654 2654
Size: 5069
EarI CTCTTCn'nnn_
Cuts at: 347 1135 1706 3092 3302 347
Size: 788 571 1386 210 2114
Fragments arranged by size:
2114 1386 788 571 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1129 3277 3587 3733 4879 1129
Size: 2148 310 146 1146 1319
Fragments arranged by size:
2148 1319 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2081 2081
Size: 5069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 436 646 994 1714 1906 1957 1989 2894
Size: 123 210 348 720 192 51 32 905
Cuts at: 2894 3326 4073 4341 313
Size: 432 747 268 1041
Fragments arranged by size:
1041 905 747 720 432 348 268 210
192 123 51 32
EcoO109I rG'GnC_Cy
Cuts at: 142 255 816 903 1272 1568 2208 142
Size: 113 561 87 369 296 640 3003
Fragments arranged by size:
3003 640 561 369 296 113 87
EcoRI G'AATT_C
Cuts at: 2073 2073
Size: 5069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5069
FspI TGC'GCA
Cuts at: 1306 2850 1306
Size: 1544 3525
HaeII r_GCGC'y
Cuts at: 153 226 241 2503 2751 3773 4752 153
Size: 73 15 2262 248 1022 979 470
Fragments arranged by size:
2262 1022 979 470 248 73 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 913 945 1834 1866 2911 2941 2943 2973 913
Size: 32 889 32 1045 30 2 30 3009
Fragments arranged by size:
3009 1045 889 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1875 2108 1875
Size: 233 4836
KasI G'GCGC_C
Cuts at: 2499 2747 2499
Size: 248 4821
KpnI G_GTAC'C
Cuts at: 1980 2089 1980
Size: 109 4960
MluI A'CGCG_T
Cuts at: 4502 4502
Size: 5069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1637 3740 3924 1637
Size: 2103 184 2782
Fragments arranged by size:
2782 2103 184
MscI TGG'CCA
Cuts at: 562 1069 2830 562
Size: 507 1761 2801
Fragments arranged by size:
2801 1761 507
MslI CAynn'nnrTG
Cuts at: 337 913 1054 1666 3058 3185 337
Size: 576 141 612 1392 127 2221
Fragments arranged by size:
2221 1392 612 576 141 127
MspA1I CmG'CkG
Cuts at: 528 660 676 1344 2180 2494 2854 3867 4112
Size: 132 16 668 836 314 360 1013 245
Cuts at: 4112 528
Size: 1485
Fragments arranged by size:
1485 1013 836 668 360 314 245 132
16
NaeI GCC'GGC
Cuts at: 154 3250 154
Size: 3096 1973
NarI GG'CG_CC
Cuts at: 2500 2748 2500
Size: 248 4821
NcoI C'CATG_G
Cuts at: 125 666 125
Size: 541 4528
NgoMIV G'CCGG_C
Cuts at: 152 3248 152
Size: 3096 1973
NspI r_CATG'y
Cuts at: 777 891 918 2122 2622 3529 777
Size: 114 27 1204 500 907 2317
Fragments arranged by size:
2317 1204 907 500 114 27
PasI CC'CwG_GG
Cuts at: 899 1830 899
Size: 931 4138
PciI A'CATG_T
Cuts at: 3525 3525
Size: 5069
PfoI T'CCnGG_A
Cuts at: 1934 1934
Size: 5069
PmeI GTTT'AAAC
Cuts at: 2068 2068
Size: 5069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 5037
PpuMI rG'GwC_Cy
Cuts at: 142 255 816 142
Size: 113 561 4395
Fragments arranged by size:
4395 561 113
PshAI GACnn'nnGTC
Cuts at: 1762 1762
Size: 5069
PspOMI G'GGCC_C
Cuts at: 902 1272 902
Size: 370 4699
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1579 1611 1579
Size: 32 5037
PstI C_TGCA'G
Cuts at: 533 1975 2116 2801 533
Size: 1442 141 685 2801
Fragments arranged by size:
2801 1442 685 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5069
PvuII CAG'CTG
Cuts at: 528 660 676 2494 2854 528
Size: 132 16 1818 360 2743
Fragments arranged by size:
2743 1818 360 132 16
RsrII CG'GwC_CG
Cuts at: 3264 3264
Size: 5069
SacI G_AGCT'C
Cuts at: 2083 2083
Size: 5069
SalI G'TCGA_C
Cuts at: 1873 2106 1873
Size: 233 4836
SanDI GG'GwC_CC
Cuts at: 142 255 142
Size: 113 4956
SapI GCTCTTCn'nnn_
Cuts at: 3092 3302 3092
Size: 210 4859
SbfI CC_TGCA'GG
Cuts at: 1975 2116 1975
Size: 141 4928
ScaI AGT'ACT
Cuts at: 4586 4586
Size: 5069
SfcI C'TryA_G
Cuts at: 33 529 1155 1971 2112 2797 3790 3981 33
Size: 496 626 816 141 685 993 191 1121
Fragments arranged by size:
1121 993 816 685 626 496 191 141
SfiI GGCCn_nnn'nGGCC
Cuts at: 1271 1271
Size: 5069
SfoI GGC'GCC
Cuts at: 2501 2749 2501
Size: 248 4821
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
SmaI CCC'GGG
Cuts at: 176 376 404 543 1858 2091 176
Size: 200 28 139 1315 233 3154
Fragments arranged by size:
3154 1315 233 200 139 28
SmlI C'TyrA_G
Cuts at: 2224 3139 3631 3893 2224
Size: 915 492 262 3400
Fragments arranged by size:
3400 915 492 262
SnaBI TAC'GTA
Cuts at: 2061 2061
Size: 5069
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5069
SphI G_CATG'C
Cuts at: 891 2122 2622 891
Size: 1231 500 3338
Fragments arranged by size:
3338 1231 500
SrfI GCCC'GGGC
Cuts at: 176 543 176
Size: 367 4702
StuI AGG'CCT
Cuts at: 205 205
Size: 5069
StyI C'CwwG_G
Cuts at: 125 206 666 1463 2203 2557 125
Size: 81 460 797 740 354 2637
Fragments arranged by size:
2637 797 740 460 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1096 1117 1426 2652 2785 3244 3453 1096
Size: 21 309 1226 133 459 209 2712
Fragments arranged by size:
2712 1226 459 309 209 133 21
TatI w'GTAC_w
Cuts at: 601 1478 4584 601
Size: 877 3106 1086
Fragments arranged by size:
3106 1086 877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 489 521 619 651 1901 1933 4586 4618 489
Size: 32 98 32 1250 32 2653 32 940
Fragments arranged by size:
2653 1250 940 98 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2866 2866
Size: 5069
XbaI T'CTAG_A
Cuts at: 2100 2100
Size: 5069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 664 915 1751 2564 664
Size: 251 836 813 3169
Fragments arranged by size:
3169 836 813 251
XmaI C'CCGG_G
Cuts at: 174 374 402 541 1856 2089 174
Size: 200 28 139 1315 233 3154
Fragments arranged by size:
3154 1315 233 200 139 28
XmnI GAAnn'nnTTC
Cuts at: 1198 1657 1198
Size: 459 4610
ZraI GAC'GTC
Cuts at: 1764 2001 1764
Size: 237 4832
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BsrGI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PsiI PspXI SacII SexAI SgrAI SspI SwaI XhoI