Restriction map for
pF1KSDA0154
(Circular) (Six-base) MAPSORT of: pF1KSDA0154.seq Check: 750 from: 1 to: 5207
pF1KSDA0154 5207 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1289 2052 1289
Size: 763 4444
AccI GT'mk_AC
Cuts at: 2245 2245
Size: 5207
Acc65I G'GTAC_C
Cuts at: 2223 2223
Size: 5207
AclI AA'CG_TT
Cuts at: 5047 5047
Size: 5207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1299 2104 3032 3464 4211 4479 460
Size: 839 805 928 432 747 268 1188
Fragments arranged by size:
1188 928 839 805 747 432 268
AflIII A'CryG_T
Cuts at: 674 2559 3663 4640 674
Size: 1885 1104 977 1241
Fragments arranged by size:
1885 1241 1104 977
AgeI A'CCGG_T
Cuts at: 3595 3595
Size: 5207
AhdI GACnn_n'nnGTC
Cuts at: 342 665 342
Size: 323 4884
AleI CACnn'nnGTG
Cuts at: 2146 2146
Size: 5207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1798 1972 1798
Size: 174 5033
AlwNI CAG_nnn'CTG
Cuts at: 321 1429 1528 1719 2512 4079 321
Size: 1108 99 191 793 1567 1449
Fragments arranged by size:
1567 1449 1108 793 191 99
ApaI G_GGCC'C
Cuts at: 1166 1697 1166
Size: 531 4676
ApaLI G'TGCA_C
Cuts at: 3977 3977
Size: 5207
ApoI r'AATT_y
Cuts at: 2211 4560 4816 2211
Size: 2349 256 2602
Fragments arranged by size:
2602 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5207
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5207
AvaI C'yCGr_G
Cuts at: 887 1614 2227 887
Size: 727 613 3867
Fragments arranged by size:
3867 727 613
BamHI G'GATC_C
Cuts at: 647 2232 647
Size: 1585 3622
BanI G'GyrC_C
Cuts at: 1277 1345 1532 2223 2637 2885 2920 1277
Size: 68 187 691 414 248 35 3564
Fragments arranged by size:
3564 691 414 248 187 68 35
BanII G_rGCy'C
Cuts at: 1166 1468 1520 1697 1763 2221 3251 1166
Size: 302 52 177 66 458 1030 3122
Fragments arranged by size:
3122 1030 458 302 177 66 52
BbeI G_GCGC'C
Cuts at: 1349 2641 2889 1349
Size: 1292 248 3667
Fragments arranged by size:
3667 1292 248
BbsI GAAGACnn'nnnn_
Cuts at: 364 641 364
Size: 277 4930
BbvCI CC'TCA_GC
Cuts at: 1374 1374
Size: 5207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 987 1021 987
Size: 34 5173
BciVI GTATCCnnnnn_n'
Cuts at: 3105 3866 5207 3105
Size: 761 1341 3105
Fragments arranged by size:
3105 1341 761
BclI T'GATC_A
Cuts at: 225 783 2727 225
Size: 558 1944 2705
Fragments arranged by size:
2705 1944 558
BglII A'GATC_T
Cuts at: 2722 4759 2722
Size: 2037 3170
BlpI GC'TnA_GC
Cuts at: 2137 2319 2137
Size: 182 5025
Bme1580I G_kGCm'C
Cuts at: 1166 1535 1697 1787 1815 1973 2832 2925 3981
Size: 369 162 90 28 158 859 93 1056
Cuts at: 3981 1166
Size: 2392
Fragments arranged by size:
2392 1056 859 369 162 158 93 90
28
BmgBI CAC'GTC
Cuts at: 1896 1896
Size: 5207
BmrI ACTGGGnnnn_n'
Cuts at: 210 1451 2419 2500 2835 4979 210
Size: 1241 968 81 335 2144 438
Fragments arranged by size:
2144 1241 968 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3574 3574
Size: 5207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 298 1270 1351 1505 172
Size: 126 972 81 154 3874
Fragments arranged by size:
3874 972 154 126 81
Bpu10I CC'TnA_GC
Cuts at: 1374 1757 1374
Size: 383 4824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1371 2383 3262 3754 4052 1371
Size: 1012 879 492 298 2526
Fragments arranged by size:
2526 1012 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4911 4911
Size: 5207
BsaAI yAC'GTr
Cuts at: 1782 2199 2560 1782
Size: 417 361 4429
Fragments arranged by size:
4429 417 361
BsaBI GATnn'nnATC
Cuts at: 6 2721 4758 6
Size: 2715 2037 455
Fragments arranged by size:
2715 2037 455
BsaHI Gr'CG_yC
Cuts at: 425 1346 2638 2886 3588 5084 425
Size: 921 1292 248 702 1496 548
Fragments arranged by size:
1496 1292 921 702 548 248
BsaWI w'CCGG_w
Cuts at: 2396 2917 3595 3869 4016 4427 2396
Size: 521 678 274 147 411 3176
Fragments arranged by size:
3176 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 1171 1201 868
Size: 30 273 30 4874
Fragments arranged by size:
4874 273 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 556 1065 1125 1216 1349 1374 1458 556
Size: 509 60 91 133 25 84 4305
Fragments arranged by size:
4305 509 133 91 84 60 25
BseYI C'CCAG_C
Cuts at: 1056 1165 1230 1261 1422 1437 1494 1662 2632
Size: 109 65 31 161 15 57 168 970
Cuts at: 2632 3390 3967 4463 1056
Size: 758 577 496 1800
Fragments arranged by size:
1800 970 758 577 496 168 161 109
65 57 31 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 655 1005 1549 478
Size: 177 350 544 4136
Fragments arranged by size:
4136 544 350 177
BsiEI CG_ry'CG
Cuts at: 104 2749 2795 4003 104
Size: 2645 46 1208 1308
Fragments arranged by size:
2645 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1763 2143 2221 2999 3189 3981 1763
Size: 380 78 778 190 792 2989
Fragments arranged by size:
2989 792 778 380 190 78
Bsp1286I G_dGCh'C
Cuts at: 1166 1468 1520 1535 1697 1763 1787 1815 1973
Size: 302 52 15 162 66 24 28 158
Cuts at: 1973 2143 2221 2832 2925 2999 3189 3251 3981
Size: 170 78 611 93 74 190 62 730
Cuts at: 3981 1166
Size: 2392
Fragments arranged by size:
2392 730 611 302 190 170 162 158
93 78 74 66 62 52 28 24
15
BspEI T'CCGG_A
Cuts at: 4427 4427
Size: 5207
BspHI T'CATG_A
Cuts at: 4383 4383
Size: 5207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 915 1289 2052 2257 2471 2773 3154 602
Size: 313 374 763 205 214 302 381 2655
Fragments arranged by size:
2655 763 381 374 313 302 214 205
BsrBI CCG'CTC
Cuts at: 50 1133 1707 3499 3553 5037 5202 50
Size: 1083 574 1792 54 1484 165 55
Fragments arranged by size:
1792 1484 1083 574 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3119 3119
Size: 5207
BsrFI r'CCGG_y
Cuts at: 3205 3386 3595 3205
Size: 181 209 4817
Fragments arranged by size:
4817 209 181
BsrGI T'GTAC_A
Cuts at: 380 380
Size: 5207
BssHII G'CGCG_C
Cuts at: 1559 1559
Size: 5207
BssSI C'ACGA_G
Cuts at: 3478 3836 3478
Size: 358 4849
BstAPI GCAn_nnn'nTGC
Cuts at: 2584 2584
Size: 5207
BstBI TT'CG_AA
Cuts at: 110 3568 110
Size: 3458 1749
BstEII G'GTnAC_C
Cuts at: 657 657
Size: 5207
BstXI CCAn_nnnn'nTGG
Cuts at: 302 302
Size: 5207
BstYI r'GATC_y
Cuts at: 1 284 647 2232 2699 2722 3057 3303 4315
Size: 283 363 1585 467 23 335 246 1012
Cuts at: 4315 4401 4413 4759 1
Size: 86 12 346 449
Fragments arranged by size:
1585 1012 467 449 363 346 335 283
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1077 1113 1077
Size: 36 5171
BtgI C'CryG_G
Cuts at: 125 392 623 1123 1536 1688 125
Size: 267 231 500 413 152 3644
Fragments arranged by size:
3644 500 413 267 231 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3157 3338 3501 3157
Size: 181 163 4863
Fragments arranged by size:
4863 181 163
BtsI GCAGTG_nn'
Cuts at: 1405 1815 1973 2450 1405
Size: 410 158 477 4162
Fragments arranged by size:
4162 477 410 158
ClaI AT'CG_AT
Cuts at: 1795 1795
Size: 5207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 461 496 461
Size: 35 5172
DraI TTT'AAA
Cuts at: 816 2206 816
Size: 1390 3817
DrdI GACnn_nn'nnGTC
Cuts at: 2913 3771 2913
Size: 858 4349
EaeI y'GGCC_r
Cuts at: 1769 2072 2792 2966 3357 3384 4729 1769
Size: 303 720 174 391 27 1345 2247
Fragments arranged by size:
2247 1345 720 391 303 174 27
EagI C'GGCC_G
Cuts at: 2792 2792
Size: 5207
EarI CTCTTCn'nnn_
Cuts at: 436 750 1592 1714 1861 3230 3440 436
Size: 314 842 122 147 1369 210 2203
Fragments arranged by size:
2203 1369 842 314 210 147 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 151 819 3415 3725 3871 5017 145
Size: 6 668 2596 310 146 1146 335
Fragments arranged by size:
2596 1146 668 335 310 146 6
EcoICRI GAG'CTC
Cuts at: 1761 2219 1761
Size: 458 4749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 298 460 1270 1299 1351 1505 2104 3032
Size: 126 162 810 29 52 154 599 928
Cuts at: 3032 3464 4211 4479 172
Size: 432 747 268 900
Fragments arranged by size:
928 900 810 747 599 432 268 162
154 126 52 29
EcoNI CCTnn'n_nnAGG
Cuts at: 598 598
Size: 5207
EcoO109I rG'GnC_Cy
Cuts at: 318 1117 1162 1670 1693 1753 2346 318
Size: 799 45 508 23 60 593 3179
Fragments arranged by size:
3179 799 593 508 60 45 23
EcoRI G'AATT_C
Cuts at: 2211 2211
Size: 5207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2133 3226 2133
Size: 1093 4114
FspI TGC'GCA
Cuts at: 2988 2988
Size: 5207
HaeII r_GCGC'y
Cuts at: 313 1349 2641 2889 3911 4890 313
Size: 1036 1292 248 1022 979 630
Fragments arranged by size:
1292 1036 1022 979 630 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 486 518 532 564 651 683 868
Size: 32 126 32 14 32 87 32 185
Cuts at: 868 900 3049 3079 3081 3111 328
Size: 32 2149 30 2 30 2424
Fragments arranged by size:
2424 2149 185 126 87 32 32 32
32 32 30 30 14 2
HincII GTy'rAC
Cuts at: 690 2246 690
Size: 1556 3651
HindIII A'AGCT_T
Cuts at: 553 553
Size: 5207
HpaI GTT'AAC
Cuts at: 690 690
Size: 5207
KasI G'GCGC_C
Cuts at: 1345 2637 2885 1345
Size: 1292 248 3667
Fragments arranged by size:
3667 1292 248
KpnI G_GTAC'C
Cuts at: 2227 2227
Size: 5207
MluI A'CGCG_T
Cuts at: 4640 4640
Size: 5207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 209 291 1325 1376 3878 4062 209
Size: 82 1034 51 2502 184 1354
Fragments arranged by size:
2502 1354 1034 184 82 51
MscI TGG'CCA
Cuts at: 1771 2074 2968 1771
Size: 303 894 4010
Fragments arranged by size:
4010 894 303
MslI CAynn'nnrTG
Cuts at: 300 1574 1780 2146 3196 3323 300
Size: 1274 206 366 1050 127 2184
Fragments arranged by size:
2184 1274 1050 366 206 127
MspA1I CmG'CkG
Cuts at: 386 1261 1336 2136 2318 2632 2992 4005 4250
Size: 875 75 800 182 314 360 1013 245
Cuts at: 4250 386
Size: 1343
Fragments arranged by size:
1343 1013 875 800 360 314 245 182
75
NaeI GCC'GGC
Cuts at: 3388 3388
Size: 5207
NarI GG'CG_CC
Cuts at: 1346 2638 2886 1346
Size: 1292 248 3667
Fragments arranged by size:
3667 1292 248
NcoI C'CATG_G
Cuts at: 125 392 623 1536 125
Size: 267 231 913 3796
Fragments arranged by size:
3796 913 267 231
NgoMIV G'CCGG_C
Cuts at: 3386 3386
Size: 5207
NspI r_CATG'y
Cuts at: 438 2260 2760 3667 438
Size: 1822 500 907 1978
Fragments arranged by size:
1978 1822 907 500
PasI CC'CwG_GG
Cuts at: 1158 1528 1734 2124 1158
Size: 370 206 390 4241
Fragments arranged by size:
4241 390 370 206
PciI A'CATG_T
Cuts at: 3663 3663
Size: 5207
PflMI CCAn_nnn'nTGG
Cuts at: 1542 2005 1542
Size: 463 4744
PmeI GTTT'AAAC
Cuts at: 2206 2206
Size: 5207
PmlI CAC'GTG
Cuts at: 1782 1782
Size: 5207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 4346
Size: 32 5175
PpuMI rG'GwC_Cy
Cuts at: 1117 1117
Size: 5207
PspOMI G'GGCC_C
Cuts at: 1162 1693 1162
Size: 531 4676
PstI C_TGCA'G
Cuts at: 599 1961 1968 1997 2049 2254 2939 599
Size: 1362 7 29 52 205 685 2867
Fragments arranged by size:
2867 1362 685 205 52 29 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5207
PvuII CAG'CTG
Cuts at: 386 1261 2136 2632 2992 386
Size: 875 875 496 360 2601
Fragments arranged by size:
2601 875 875 496 360
RsrII CG'GwC_CG
Cuts at: 3402 3402
Size: 5207
SacI G_AGCT'C
Cuts at: 1763 2221 1763
Size: 458 4749
SalI G'TCGA_C
Cuts at: 2244 2244
Size: 5207
SapI GCTCTTCn'nnn_
Cuts at: 1714 3230 3440 1714
Size: 1516 210 3481
Fragments arranged by size:
3481 1516 210
SbfI CC_TGCA'GG
Cuts at: 599 2254 599
Size: 1655 3552
ScaI AGT'ACT
Cuts at: 4724 4724
Size: 5207
SfcI C'TryA_G
Cuts at: 33 595 1957 1964 1993 2045 2250 2935 3928
Size: 562 1362 7 29 52 205 685 993
Cuts at: 3928 4119 33
Size: 191 1121
Fragments arranged by size:
1362 1121 993 685 562 205 191 52
29 7
SfoI GGC'GCC
Cuts at: 1347 2639 2887 1347
Size: 1292 248 3667
Fragments arranged by size:
3667 1292 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5207
SmaI CCC'GGG
Cuts at: 889 2229 889
Size: 1340 3867
SmlI C'TyrA_G
Cuts at: 1386 2362 3277 3769 4031 1386
Size: 976 915 492 262 2562
Fragments arranged by size:
2562 976 915 492 262
SnaBI TAC'GTA
Cuts at: 2199 2199
Size: 5207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5207
SphI G_CATG'C
Cuts at: 2260 2760 2260
Size: 500 4707
StuI AGG'CCT
Cuts at: 1111 1111
Size: 5207
StyI C'CwwG_G
Cuts at: 125 362 392 623 993 1499 1536 1868 2341
Size: 237 30 231 370 506 37 332 473
Cuts at: 2341 2695 125
Size: 354 2637
Fragments arranged by size:
2637 506 473 370 354 332 237 231
37 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2071 2790 2923 3382 3591 2071
Size: 719 133 459 209 3687
Fragments arranged by size:
3687 719 459 209 133
TatI w'GTAC_w
Cuts at: 380 4722 380
Size: 4342 865
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1709 1741 4724 4756 1709
Size: 32 2983 32 2160
Fragments arranged by size:
2983 2160 32 32
Tth111I GACn'n_nGTC
Cuts at: 3004 3004
Size: 5207
XbaI T'CTAG_A
Cuts at: 1593 2238 1593
Size: 645 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2702 2702
Size: 5207
XmaI C'CCGG_G
Cuts at: 887 2227 887
Size: 1340 3867
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BfrBI
BglI BmtI BsiWI BsmI BsmBI BstZ17I DraIII EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI XmnI
ZraI