Restriction map for
pF1KSDA0157
(Circular) (Six-base) MAPSORT of: pF1KSDA0157.seq Check: 516 from: 1 to: 4382
pF1KSDA0157 4382 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 235 4222 235
Size: 3987 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 498 552 638 757 1016 1192 2207 2639
Size: 360 54 86 119 259 176 1015 432
Cuts at: 2639 3386 3654 138
Size: 747 268 866
Fragments arranged by size:
1015 866 747 432 360 268 259 176
119 86 54
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4382
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 943 975 943
Size: 32 4350
AlwNI CAG_nnn'CTG
Cuts at: 1687 3254 1687
Size: 1567 2815
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 271 1260 1386 3735 3991 271
Size: 989 126 2349 256 662
Fragments arranged by size:
2349 989 662 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1159 1192 1159
Size: 33 4349
BamHI G'GATC_C
Cuts at: 1032 1407 1032
Size: 375 4007
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 1141 1396 2426 1141
Size: 255 1030 3097
Fragments arranged by size:
3097 1030 255
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 644 867 644
Size: 223 4159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1010 1044 1010
Size: 34 4348
BciVI GTATCCnnnnn_n'
Cuts at: 1187 2280 3041 4382 1187
Size: 1093 761 1341 1187
Fragments arranged by size:
1341 1187 1093 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 494 2007 2100 3156 494
Size: 1513 93 1056 1720
Fragments arranged by size:
1720 1513 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 220 1594 1675 2010 4154 220
Size: 1374 81 335 2144 448
Fragments arranged by size:
2144 1374 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1109 2749 1109
Size: 1640 2742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1241 1241
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1558 2437 2929 3227 1112
Size: 446 879 492 298 2267
Fragments arranged by size:
2267 879 492 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 1374 1735 1374
Size: 361 4021
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 133 1813 2061 2763 4259 133
Size: 1680 248 702 1496 256
Fragments arranged by size:
1680 1496 702 256 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 1028 1193 370
Size: 658 165 3559
Fragments arranged by size:
3559 658 165
BseYI C'CCAG_C
Cuts at: 954 1807 2565 3142 3638 954
Size: 853 758 577 496 1698
Fragments arranged by size:
1698 853 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 818
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 566 1396 2174 2364 3156 566
Size: 830 778 190 792 1792
Fragments arranged by size:
1792 830 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 494 566 1141 1396 2007 2100 2174 2364 2426
Size: 72 575 255 611 93 74 190 62
Cuts at: 2426 3156 494
Size: 730 1720
Fragments arranged by size:
1720 730 611 575 255 190 93 74
72 62
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 444 1432 1646 1948 2329 444
Size: 988 214 302 381 2497
Fragments arranged by size:
2497 988 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 796 1759 796
Size: 963 3419
BstBI TT'CG_AA
Cuts at: 110 2743 110
Size: 2633 1749
BstYI r'GATC_y
Cuts at: 1 1032 1407 1874 1897 2232 2478 3490 3576
Size: 1031 375 467 23 335 246 1012 86
Cuts at: 3576 3588 3934 1
Size: 12 346 449
Fragments arranged by size:
1031 1012 467 449 375 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1322 125
Size: 1197 3185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 527 797 923 1625 527
Size: 270 126 702 3284
Fragments arranged by size:
3284 702 270 126
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 1967 2141 2532 2559 3904 1967
Size: 174 391 27 1345 2445
Fragments arranged by size:
2445 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 517 571 1146 2405 2615 517
Size: 54 575 1259 210 2284
Fragments arranged by size:
2284 1259 575 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 498 552 638 757 1016 1192 1241 2207
Size: 360 54 86 119 259 176 49 966
Cuts at: 2207 2639 3386 3654 138
Size: 432 747 268 866
Fragments arranged by size:
966 866 747 432 360 268 259 176
119 86 54 49
EcoO109I rG'GnC_Cy
Cuts at: 1341 1521 1341
Size: 180 4202
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
EcoRV GAT'ATC
Cuts at: 599 599
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 179 1816 2064 3086 4065 179
Size: 1637 248 1022 979 496
Fragments arranged by size:
1637 1022 979 496 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2224 2254 2256 2286 2224
Size: 30 2 30 4320
Fragments arranged by size:
4320 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
HindIII A'AGCT_T
Cuts at: 945 945
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 2371 2498 2371
Size: 127 4255
MspA1I CmG'CkG
Cuts at: 994 1219 1493 1807 2167 3180 3425 994
Size: 225 274 314 360 1013 245 1951
Fragments arranged by size:
1951 1013 360 314 274 245 225
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 125 1322 125
Size: 1197 3185
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 1435 1935 2842 1435
Size: 500 907 2975
Fragments arranged by size:
2975 907 500
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PfoI T'CCnGG_A
Cuts at: 1179 1179
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 440 472 3521 3553 440
Size: 32 3049 32 1269
Fragments arranged by size:
3049 1269 32 32
PpuMI rG'GwC_Cy
Cuts at: 1341 1341
Size: 4382
PsiI TTA'TAA
Cuts at: 764 764
Size: 4382
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 994 1219 1807 2167 994
Size: 225 588 360 3209
Fragments arranged by size:
3209 588 360 225
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 571 1146 2405 2615 571
Size: 575 1259 210 2338
Fragments arranged by size:
2338 1259 575 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 1080 3899 1080
Size: 2819 1563
SfcI C'TryA_G
Cuts at: 33 444 1274 1425 2110 3103 3294 33
Size: 411 830 151 685 993 191 1121
Fragments arranged by size:
1121 993 830 685 411 191 151
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 1127 1537 2452 2944 3206 1127
Size: 410 915 492 262 2303
Fragments arranged by size:
2303 915 492 410 262
SnaBI TAC'GTA
Cuts at: 1374 1374
Size: 4382
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StuI AGG'CCT
Cuts at: 1027 1027
Size: 4382
StyI C'CwwG_G
Cuts at: 125 1322 1516 1870 125
Size: 1197 194 354 2637
Fragments arranged by size:
2637 1197 354 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 392 1076 1965 2098 2557 2766 392
Size: 684 889 133 459 209 2008
Fragments arranged by size:
2008 889 684 459 209 133
TatI w'GTAC_w
Cuts at: 641 1006 1078 3897 641
Size: 365 72 2819 1126
Fragments arranged by size:
2819 1126 365 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 499 2179 499
Size: 1680 2702
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1216 1877 1216
Size: 661 3721
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI XmnI
ZraI