Restriction map for
pF1KSDA0158
(Circular) (Six-base) MAPSORT of: pF1KSDA0158.seq Check: 4609 from: 1 to: 4220
pF1KSDA0158 4220 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1258 1258
Size: 4220
Acc65I G'GTAC_C
Cuts at: 509 1236 509
Size: 727 3493
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 730 967 2045 2477 3224 3492 712
Size: 18 237 1078 432 747 268 1440
Fragments arranged by size:
1440 1078 747 432 268 237 18
AflII C'TTAA_G
Cuts at: 604 604
Size: 4220
AflIII A'CryG_T
Cuts at: 1205 1572 2676 3653 1205
Size: 367 1104 977 1772
Fragments arranged by size:
1772 1104 977 367
AgeI A'CCGG_T
Cuts at: 2608 2608
Size: 4220
AlwNI CAG_nnn'CTG
Cuts at: 1525 3092 1525
Size: 1567 2653
ApaLI G'TGCA_C
Cuts at: 2990 2990
Size: 4220
ApoI r'AATT_y
Cuts at: 1224 3573 3829 1224
Size: 2349 256 1615
Fragments arranged by size:
2349 1615 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 1195 1240 1195
Size: 45 4175
BamHI G'GATC_C
Cuts at: 847 1245 847
Size: 398 3822
BanI G'GyrC_C
Cuts at: 509 1199 1236 1650 1898 1933 509
Size: 690 37 414 248 35 2796
Fragments arranged by size:
2796 690 414 248 37 35
BanII G_rGCy'C
Cuts at: 1112 1194 1234 2264 1112
Size: 82 40 1030 3068
Fragments arranged by size:
3068 1030 82 40
BbeI G_GCGC'C
Cuts at: 1654 1902 1654
Size: 248 3972
BciVI GTATCCnnnnn_n'
Cuts at: 2118 2879 4220 2118
Size: 761 1341 2118
Fragments arranged by size:
2118 1341 761
BclI T'GATC_A
Cuts at: 1740 1740
Size: 4220
BglII A'GATC_T
Cuts at: 1085 1735 3772 1085
Size: 650 2037 1533
Fragments arranged by size:
2037 1533 650
BlpI GC'TnA_GC
Cuts at: 1106 1332 1106
Size: 226 3994
Bme1580I G_kGCm'C
Cuts at: 1202 1845 1938 2994 1202
Size: 643 93 1056 2428
Fragments arranged by size:
2428 1056 643 93
BmrI ACTGGGnnnn_n'
Cuts at: 1432 1513 1848 3992 1432
Size: 81 335 2144 1660
Fragments arranged by size:
2144 1660 335 81
BmtI G_CTAG'C
Cuts at: 827 827
Size: 4220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 910 2587 910
Size: 1677 2543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 970 353
Size: 617 3603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1021 1396 2275 2767 3065 802
Size: 219 375 879 492 298 1957
Fragments arranged by size:
1957 879 492 375 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 3924 3924
Size: 4220
BsaAI yAC'GTr
Cuts at: 1206 1212 1573 1206
Size: 6 361 3853
Fragments arranged by size:
3853 361 6
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 1651 1899 2601 4097 1651
Size: 248 702 1496 1774
Fragments arranged by size:
1774 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1409 1930 2608 2882 3029 3440 1409
Size: 521 678 274 147 411 2189
Fragments arranged by size:
2189 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1645 2403 2980 3476 1645
Size: 758 577 496 2389
Fragments arranged by size:
2389 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1762 1808 3016 104
Size: 1658 46 1208 1308
Fragments arranged by size:
1658 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 935 1112 1234 2012 2202 2994 935
Size: 177 122 778 190 792 2161
Fragments arranged by size:
2161 792 778 190 177 122
Bsp1286I G_dGCh'C
Cuts at: 935 1112 1194 1202 1234 1845 1938 2012 2202
Size: 177 82 8 32 611 93 74 190
Cuts at: 2202 2264 2994 935
Size: 62 730 2161
Fragments arranged by size:
2161 730 611 190 177 93 82 74
62 32 8
BspEI T'CCGG_A
Cuts at: 3440 3440
Size: 4220
BspHI T'CATG_A
Cuts at: 3396 3396
Size: 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 1270 1484 1786 2167 521
Size: 749 214 302 381 2574
Fragments arranged by size:
2574 749 381 302 214
BsrBI CCG'CTC
Cuts at: 50 527 705 2512 2566 4050 4215 50
Size: 477 178 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 477 178 165 55 54
BsrDI GCAATG_nn'
Cuts at: 665 2132 665
Size: 1467 2753
BsrFI r'CCGG_y
Cuts at: 2218 2399 2608 2218
Size: 181 209 3830
Fragments arranged by size:
3830 209 181
BssSI C'ACGA_G
Cuts at: 2491 2849 2491
Size: 358 3862
BstAPI GCAn_nnn'nTGC
Cuts at: 1597 1597
Size: 4220
BstBI TT'CG_AA
Cuts at: 110 2581 110
Size: 2471 1749
BstEII G'GTnAC_C
Cuts at: 993 993
Size: 4220
BstYI r'GATC_y
Cuts at: 1 847 981 1085 1245 1712 1735 2070 2316
Size: 846 134 104 160 467 23 335 246
Cuts at: 2316 3328 3414 3426 3772 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 846 467 449 346 335 246 160
134 104 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1196 2170 2351 2514 1196
Size: 974 181 163 2902
Fragments arranged by size:
2902 974 181 163
BtsI GCAGTG_nn'
Cuts at: 1463 1463
Size: 4220
DraI TTT'AAA
Cuts at: 798 1219 798
Size: 421 3799
DraIII CAC_nnn'GTG
Cuts at: 1206 1206
Size: 4220
DrdI GACnn_nn'nnGTC
Cuts at: 1926 2784 1926
Size: 858 3362
EaeI y'GGCC_r
Cuts at: 1805 1979 2370 2397 3742 1805
Size: 174 391 27 1345 2283
Fragments arranged by size:
2283 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 386 2243 2453 386
Size: 1857 210 2153
Fragments arranged by size:
2153 1857 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1102 2428 2738 2884 4030 1102
Size: 1326 310 146 1146 1292
Fragments arranged by size:
1326 1292 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1110 1232 1110
Size: 122 4098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 712 730 967 970 2045 2477 3224 3492
Size: 359 18 237 3 1075 432 747 268
Cuts at: 3492 353
Size: 1081
Fragments arranged by size:
1081 1075 747 432 359 268 237 18
3
EcoO109I rG'GnC_Cy
Cuts at: 1002 1359 1002
Size: 357 3863
EcoRI G'AATT_C
Cuts at: 1224 1224
Size: 4220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 4220
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 1654 1902 2924 3903 1654
Size: 248 1022 979 1971
Fragments arranged by size:
1971 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 2062 2092 2094 2124 760
Size: 32 1270 30 2 30 2856
Fragments arranged by size:
2856 1270 32 30 30 2
HincII GTy'rAC
Cuts at: 1259 1259
Size: 4220
KasI G'GCGC_C
Cuts at: 1650 1898 1650
Size: 248 3972
KpnI G_GTAC'C
Cuts at: 513 1240 513
Size: 727 3493
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 159 825 2891 3075 159
Size: 666 2066 184 1304
Fragments arranged by size:
2066 1304 666 184
MscI TGG'CCA
Cuts at: 1981 1981
Size: 4220
MslI CAynn'nnrTG
Cuts at: 244 973 2209 2336 244
Size: 729 1236 127 2128
Fragments arranged by size:
2128 1236 729 127
MspA1I CmG'CkG
Cuts at: 1331 1645 2005 3018 3263 1331
Size: 314 360 1013 245 2288
Fragments arranged by size:
2288 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 4220
NarI GG'CG_CC
Cuts at: 1651 1899 1651
Size: 248 3972
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 4220
NheI G'CTAG_C
Cuts at: 823 823
Size: 4220
NspI r_CATG'y
Cuts at: 978 1122 1273 1773 2680 978
Size: 144 151 500 907 2518
Fragments arranged by size:
2518 907 500 151 144
PasI CC'CwG_GG
Cuts at: 902 902
Size: 4220
PciI A'CATG_T
Cuts at: 2676 2676
Size: 4220
PfoI T'CCnGG_A
Cuts at: 986 986
Size: 4220
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PmlI CAC'GTG
Cuts at: 1206 1206
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 4188
PpuMI rG'GwC_Cy
Cuts at: 1002 1002
Size: 4220
PsiI TTA'TAA
Cuts at: 154 154
Size: 4220
PstI C_TGCA'G
Cuts at: 460 1267 1952 460
Size: 807 685 2728
Fragments arranged by size:
2728 807 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 1645 2005 1645
Size: 360 3860
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 1112 1234 1112
Size: 122 4098
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 386 2243 2453 386
Size: 1857 210 2153
Fragments arranged by size:
2153 1857 210
SbfI CC_TGCA'GG
Cuts at: 1267 1267
Size: 4220
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 456 1263 1948 2941 3132 33
Size: 423 807 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 423 191
SfoI GGC'GCC
Cuts at: 1652 1900 1652
Size: 248 3972
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 1242 1242
Size: 4220
SmlI C'TyrA_G
Cuts at: 604 817 1036 1375 2290 2782 3044 604
Size: 213 219 339 915 492 262 1780
Fragments arranged by size:
1780 915 492 339 262 219 213
SnaBI TAC'GTA
Cuts at: 1212 1212
Size: 4220
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1122 1273 1773 1122
Size: 151 500 3569
Fragments arranged by size:
3569 500 151
SspI AAT'ATT
Cuts at: 654 654
Size: 4220
StyI C'CwwG_G
Cuts at: 1354 1708 1354
Size: 354 3866
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 985 1803 1936 2395 2604 985
Size: 818 133 459 209 2601
Fragments arranged by size:
2601 818 459 209 133
TatI w'GTAC_w
Cuts at: 3735 3735
Size: 4220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4188
Tth111I GACn'n_nGTC
Cuts at: 2017 2017
Size: 4220
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 1715
Size: 4220
XmaI C'CCGG_G
Cuts at: 1240 1240
Size: 4220
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PflMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI XmnI ZraI