Restriction map for
pF1KSDA0161
(Circular) (Six-base) MAPSORT of: pF1KSDA0161.seq Check: 7638 from: 1 to: 4015
pF1KSDA0161 4015 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 478 568 478
Size: 90 3925
AccI GT'mk_AC
Cuts at: 695 1051 695
Size: 356 3659
Acc65I G'GTAC_C
Cuts at: 140 1029 140
Size: 889 3126
AclI AA'CG_TT
Cuts at: 3855 3855
Size: 4015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1838 2270 3019 3287 1838
Size: 432 749 268 2566
Fragments arranged by size:
2566 749 432 268
AfeI AGC'GCT
Cuts at: 676 676
Size: 4015
AflIII A'CryG_T
Cuts at: 1365 2471 3448 1365
Size: 1106 977 1932
Fragments arranged by size:
1932 1106 977
AgeI A'CCGG_T
Cuts at: 2403 2403
Size: 4015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 333 333
Size: 4015
AlwNI CAG_nnn'CTG
Cuts at: 328 1318 2887 328
Size: 990 1569 1456
Fragments arranged by size:
1569 1456 990
ApaLI G'TGCA_C
Cuts at: 2785 2785
Size: 4015
ApoI r'AATT_y
Cuts at: 547 1017 3368 3624 547
Size: 470 2351 256 938
Fragments arranged by size:
2351 938 470 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4015
AvaI C'yCGr_G
Cuts at: 622 842 1033 622
Size: 220 191 3604
Fragments arranged by size:
3604 220 191
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4015
BanI G'GyrC_C
Cuts at: 140 455 575 1029 1443 1691 1726 140
Size: 315 120 454 414 248 35 2429
Fragments arranged by size:
2429 454 414 315 248 120 35
BanII G_rGCy'C
Cuts at: 284 1027 2057 284
Size: 743 1030 2242
Fragments arranged by size:
2242 1030 743
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2674 4015 1911
Size: 763 1341 1911
Fragments arranged by size:
1911 1341 763
BclI T'GATC_A
Cuts at: 287 1533 287
Size: 1246 2769
BfrBI ATG'CAT
Cuts at: 245 245
Size: 4015
BglII A'GATC_T
Cuts at: 1528 3567 1528
Size: 2039 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4015
Bme1580I G_kGCm'C
Cuts at: 460 1638 1731 2789 460
Size: 1178 93 1058 1686
Fragments arranged by size:
1686 1178 1058 93
BmgBI CAC'GTC
Cuts at: 498 498
Size: 4015
BmrI ACTGGGnnnn_n'
Cuts at: 202 228 1225 1306 1641 3787 202
Size: 26 997 81 335 2146 430
Fragments arranged by size:
2146 997 430 335 81 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 790 475
Size: 315 3700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2562 2860 1189
Size: 879 494 298 2344
Fragments arranged by size:
2344 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 592 622 3719 592
Size: 30 3097 888
Fragments arranged by size:
3097 888 30
BsaAI yAC'GTr
Cuts at: 1005 1366 1005
Size: 361 3654
BsaBI GATnn'nnATC
Cuts at: 6 1527 3566 6
Size: 1521 2039 455
Fragments arranged by size:
2039 1521 455
BsaHI Gr'CG_yC
Cuts at: 463 1444 1692 2396 3892 463
Size: 981 248 704 1496 586
Fragments arranged by size:
1496 981 704 586 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2403 2677 2824 3235 1202
Size: 521 680 274 147 411 1982
Fragments arranged by size:
1982 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 515 831 1438 2196 2775 3271 515
Size: 316 607 758 579 496 1259
Fragments arranged by size:
1259 758 607 579 496 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 990 544
Size: 446 3569
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2811 104
Size: 1451 46 1210 1308
Fragments arranged by size:
1451 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 284 1027 1805 1995 2789 284
Size: 743 778 190 794 1510
Fragments arranged by size:
1510 794 778 743 190
BsmBI CGTCTCn'nnnn_
Cuts at: 701 701
Size: 4015
Bsp1286I G_dGCh'C
Cuts at: 284 460 1027 1638 1731 1805 1995 2057 2789
Size: 176 567 611 93 74 190 62 732
Cuts at: 2789 284
Size: 1510
Fragments arranged by size:
1510 732 611 567 190 176 93 74
62
BspEI T'CCGG_A
Cuts at: 3235 3235
Size: 4015
BspHI T'CATG_A
Cuts at: 3191 3191
Size: 4015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 478 568 1063 1277 1579 1960 478
Size: 90 495 214 302 381 2533
Fragments arranged by size:
2533 495 381 302 214 90
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3845 4010 50
Size: 2255 54 1486 165 55
Fragments arranged by size:
2255 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1925 1925
Size: 4015
BsrFI r'CCGG_y
Cuts at: 143 2011 2192 2403 143
Size: 1868 181 211 1755
Fragments arranged by size:
1868 1755 211 181
BssHII G'CGCG_C
Cuts at: 719 719
Size: 4015
BssSI C'ACGA_G
Cuts at: 2284 2644 2284
Size: 360 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 536 740 1390 536
Size: 204 650 3161
Fragments arranged by size:
3161 650 204
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4015
BstYI r'GATC_y
Cuts at: 1 1038 1505 1528 1863 2109 3123 3209 3221
Size: 1037 467 23 335 246 1014 86 12
Cuts at: 3221 3567 1
Size: 346 449
Fragments arranged by size:
1037 1014 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3671
Fragments arranged by size:
3671 181 163
BtsI GCAGTG_nn'
Cuts at: 532 1256 532
Size: 724 3291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 927 962 927
Size: 35 3980
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4015
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2579 1719
Size: 860 3155
EaeI y'GGCC_r
Cuts at: 582 935 1598 1772 2163 2190 3537 582
Size: 353 663 174 391 27 1347 1060
Fragments arranged by size:
1347 1060 663 391 353 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4015
EarI CTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3805
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2533 2679 3825 2221
Size: 312 146 1146 2411
Fragments arranged by size:
2411 1146 312 146
EcoICRI GAG'CTC
Cuts at: 282 1025 282
Size: 743 3272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 790 1838 2270 3019 3287 475
Size: 315 1048 432 749 268 1203
Fragments arranged by size:
1203 1048 749 432 315 268
EcoO109I rG'GnC_Cy
Cuts at: 156 814 1152 156
Size: 658 338 3019
Fragments arranged by size:
3019 658 338
EcoRI G'AATT_C
Cuts at: 547 1017 547
Size: 470 3545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 768 2032 768
Size: 1264 2751
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4015
HaeII r_GCGC'y
Cuts at: 678 1447 1695 2719 3698 678
Size: 769 248 1024 979 995
Fragments arranged by size:
1024 995 979 769 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 846 878 1855 1885 1887 1917 846
Size: 32 977 30 2 30 2944
Fragments arranged by size:
2944 977 32 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4015
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3767
KpnI G_GTAC'C
Cuts at: 144 1033 144
Size: 889 3126
MluI A'CGCG_T
Cuts at: 3448 3448
Size: 4015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 427 2686 2870 427
Size: 2259 184 1572
Fragments arranged by size:
2259 1572 184
MscI TGG'CCA
Cuts at: 584 937 1774 584
Size: 353 837 2825
Fragments arranged by size:
2825 837 353
MslI CAynn'nnrTG
Cuts at: 2002 2129 2002
Size: 127 3888
MspA1I CmG'CkG
Cuts at: 174 385 572 1124 1438 1798 2813 3058 174
Size: 211 187 552 314 360 1015 245 1131
Fragments arranged by size:
1131 1015 552 360 314 245 211 187
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4015
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3767
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4015
NruI TCG'CGA
Cuts at: 2376 2376
Size: 4015
NsiI A_TGCA'T
Cuts at: 247 247
Size: 4015
NspI r_CATG'y
Cuts at: 1066 1566 2475 1066
Size: 500 909 2606
Fragments arranged by size:
2606 909 500
PciI A'CATG_T
Cuts at: 2471 2471
Size: 4015
PflMI CCAn_nnn'nTGG
Cuts at: 498 498
Size: 4015
PfoI T'CCnGG_A
Cuts at: 491 491
Size: 4015
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3154 3186 3154
Size: 32 3983
PpuMI rG'GwC_Cy
Cuts at: 156 814 156
Size: 658 3357
PstI C_TGCA'G
Cuts at: 509 1060 1745 509
Size: 551 685 2779
Fragments arranged by size:
2779 685 551
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4015
PvuII CAG'CTG
Cuts at: 385 572 1438 1798 385
Size: 187 866 360 2602
Fragments arranged by size:
2602 866 360 187
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4015
SacI G_AGCT'C
Cuts at: 284 1027 284
Size: 743 3272
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4015
SanDI GG'GwC_CC
Cuts at: 814 814
Size: 4015
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3805
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4015
ScaI AGT'ACT
Cuts at: 3532 3532
Size: 4015
SfcI C'TryA_G
Cuts at: 33 352 505 1056 1741 2736 2927 33
Size: 319 153 551 685 995 191 1121
Fragments arranged by size:
1121 995 685 551 319 191 153
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3767
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4015
SmaI CCC'GGG
Cuts at: 624 844 1035 624
Size: 220 191 3604
Fragments arranged by size:
3604 220 191
SmlI C'TyrA_G
Cuts at: 1168 2083 2577 2839 1168
Size: 915 494 262 2344
Fragments arranged by size:
2344 915 494 262
SnaBI TAC'GTA
Cuts at: 1005 1005
Size: 4015
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4015
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3515
StyI C'CwwG_G
Cuts at: 1147 1501 1147
Size: 354 3661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 1729 2188 2399 1596
Size: 133 459 211 3212
Fragments arranged by size:
3212 459 211 133
TatI w'GTAC_w
Cuts at: 253 948 3530 253
Size: 695 2582 738
Fragments arranged by size:
2582 738 695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3532 3564 3532
Size: 32 3983
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4015
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 1508 156
Size: 1352 2663
XmaI C'CCGG_G
Cuts at: 622 842 1033 622
Size: 220 191 3604
Fragments arranged by size:
3604 220 191
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI