Restriction map for
pF1KSDA0163
(Circular) (Six-base) MAPSORT of: pF1KSDA0163.seq Check: 6775 from: 1 to: 4787
pF1KSDA0163 4787 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1024 1024
Size: 4787
AccI GT'mk_AC
Cuts at: 518 1825 518
Size: 1307 3480
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 740 817 2612 3044 3791 4059 432
Size: 308 77 1795 432 747 268 1160
Fragments arranged by size:
1795 1160 747 432 308 268 77
AflIII A'CryG_T
Cuts at: 815 934 2139 3243 4220 815
Size: 119 1205 1104 977 1382
Fragments arranged by size:
1382 1205 1104 977 119
AgeI A'CCGG_T
Cuts at: 3175 3175
Size: 4787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1690 1690
Size: 4787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 955 987 1489 1521 955
Size: 32 502 32 4221
Fragments arranged by size:
4221 502 32 32
AlwNI CAG_nnn'CTG
Cuts at: 567 606 1564 1614 2092 3659 567
Size: 39 958 50 478 1567 1695
Fragments arranged by size:
1695 1567 958 478 50 39
ApaLI G'TGCA_C
Cuts at: 3557 3557
Size: 4787
ApoI r'AATT_y
Cuts at: 1791 4140 4396 1791
Size: 2349 256 2182
Fragments arranged by size:
2349 2182 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 640 1807 640
Size: 1167 3620
AvrII C'CTAG_G
Cuts at: 459 459
Size: 4787
BamHI G'GATC_C
Cuts at: 372 1310 1812 372
Size: 938 502 3347
Fragments arranged by size:
3347 938 502
BanI G'GyrC_C
Cuts at: 129 148 244 482 860 1247 1598 1803 2217
Size: 19 96 238 378 387 351 205 414
Cuts at: 2217 2465 2500 129
Size: 248 35 2416
Fragments arranged by size:
2416 414 387 378 351 248 238 205
96 35 19
BanII G_rGCy'C
Cuts at: 1801 2831 1801
Size: 1030 3757
BbeI G_GCGC'C
Cuts at: 152 1251 1602 2221 2469 152
Size: 1099 351 619 248 2470
Fragments arranged by size:
2470 1099 619 351 248
BbsI GAAGACnn'nnnn_
Cuts at: 899 1655 899
Size: 756 4031
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 424 458 424
Size: 34 4753
BciVI GTATCCnnnnn_n'
Cuts at: 2685 3446 4787 2685
Size: 761 1341 2685
Fragments arranged by size:
2685 1341 761
BclI T'GATC_A
Cuts at: 2307 2307
Size: 4787
BglI GCCn_nnn'nGGC
Cuts at: 198 572 1636 198
Size: 374 1064 3349
Fragments arranged by size:
3349 1064 374
BglII A'GATC_T
Cuts at: 2302 4339 2302
Size: 2037 2750
BlpI GC'TnA_GC
Cuts at: 1899 1899
Size: 4787
Bme1580I G_kGCm'C
Cuts at: 134 472 828 1685 2412 2505 3561 134
Size: 338 356 857 727 93 1056 1360
Fragments arranged by size:
1360 1056 857 727 356 338 93
BmrI ACTGGGnnnn_n'
Cuts at: 549 1999 2080 2415 4559 549
Size: 1450 81 335 2144 777
Fragments arranged by size:
2144 1450 777 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 4787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 1624
Size: 4787
Bpu10I CC'TnA_GC
Cuts at: 523 1006 523
Size: 483 4304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1963 2842 3334 3632 1963
Size: 879 492 298 3118
Fragments arranged by size:
3118 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4491 4491
Size: 4787
BsaAI yAC'GTr
Cuts at: 1779 2140 1779
Size: 361 4426
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 149 1248 1599 2218 2466 3168 4664 149
Size: 1099 351 619 248 702 1496 272
Fragments arranged by size:
1496 1099 702 619 351 272 248
BsaWI w'CCGG_w
Cuts at: 1976 2497 3175 3449 3596 4007 1976
Size: 521 678 274 147 411 2756
Fragments arranged by size:
2756 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 639 669 1468 1498 1569 1599 639
Size: 30 799 30 71 30 3827
Fragments arranged by size:
3827 799 71 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 410 1182 410
Size: 772 4015
BseYI C'CCAG_C
Cuts at: 284 731 827 1713 2212 2970 3547 4043 284
Size: 447 96 886 499 758 577 496 1028
Fragments arranged by size:
1028 886 758 577 499 496 447 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 996
Size: 4787
BsiEI CG_ry'CG
Cuts at: 104 1305 2329 2375 3583 104
Size: 1201 1024 46 1208 1308
Fragments arranged by size:
1308 1208 1201 1024 46
BsiHKAI G_wGCw'C
Cuts at: 179 1711 1801 2579 2769 3561 179
Size: 1532 90 778 190 792 1405
Fragments arranged by size:
1532 1405 792 778 190 90
BsmI GAATG_Cn'
Cuts at: 742 742
Size: 4787
Bsp1286I G_dGCh'C
Cuts at: 134 179 472 828 1685 1711 1801 2412 2505
Size: 45 293 356 857 26 90 611 93
Cuts at: 2505 2579 2769 2831 3561 134
Size: 74 190 62 730 1360
Fragments arranged by size:
1360 857 730 611 356 293 190 93
90 74 62 45 26
BspEI T'CCGG_A
Cuts at: 4007 4007
Size: 4787
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 565 1024 1837 2051 2353 2734 565
Size: 459 813 214 302 381 2618
Fragments arranged by size:
2618 813 459 381 302 214
BsrBI CCG'CTC
Cuts at: 50 527 645 716 1703 3079 3133 4617 4782
Size: 477 118 71 987 1376 54 1484 165
Cuts at: 4782 50
Size: 55
Fragments arranged by size:
1484 1376 987 477 165 118 71 55
54
BsrDI GCAATG_nn'
Cuts at: 2699 2699
Size: 4787
BsrFI r'CCGG_y
Cuts at: 1232 2785 2966 3175 1232
Size: 1553 181 209 2844
Fragments arranged by size:
2844 1553 209 181
BssSI C'ACGA_G
Cuts at: 172 1678 3058 3416 172
Size: 1506 1380 358 1543
Fragments arranged by size:
1543 1506 1380 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1614 2164 1614
Size: 550 4237
BstBI TT'CG_AA
Cuts at: 110 3148 110
Size: 3038 1749
BstYI r'GATC_y
Cuts at: 1 372 1310 1812 2279 2302 2637 2883 3895
Size: 371 938 502 467 23 335 246 1012
Cuts at: 3895 3981 3993 4339 1
Size: 86 12 346 449
Fragments arranged by size:
1012 938 502 467 449 371 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 986 986
Size: 4787
BtgI C'CryG_G
Cuts at: 125 376 1020 1154 125
Size: 251 644 134 3758
Fragments arranged by size:
3758 644 251 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 280 1210 1619 1726 2030 280
Size: 930 409 107 304 3037
Fragments arranged by size:
3037 930 409 304 107
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DraIII CAC_nnn'GTG
Cuts at: 423 423
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 2493 3351 2493
Size: 858 3929
EaeI y'GGCC_r
Cuts at: 199 500 705 794 1157 1302 1449 1740 2372
Size: 301 205 89 363 145 147 291 632
Cuts at: 2372 2546 2937 2964 4309 199
Size: 174 391 27 1345 677
Fragments arranged by size:
1345 677 632 391 363 301 291 205
174 147 145 89 27
EagI C'GGCC_G
Cuts at: 1302 2372 1302
Size: 1070 3717
EarI CTCTTCn'nnn_
Cuts at: 169 759 1225 2810 3020 169
Size: 590 466 1585 210 1936
Fragments arranged by size:
1936 1585 590 466 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1457 2995 3305 3451 4597 1457
Size: 1538 310 146 1146 1647
Fragments arranged by size:
1647 1538 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1799 1799
Size: 4787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 740 817 1624 2612 3044 3791 4059 432
Size: 308 77 807 988 432 747 268 1160
Fragments arranged by size:
1160 988 807 747 432 308 268 77
EcoNI CCTnn'n_nnAGG
Cuts at: 622 622
Size: 4787
EcoO109I rG'GnC_Cy
Cuts at: 1064 1092 1628 1926 1064
Size: 28 536 298 3925
Fragments arranged by size:
3925 536 298 28
EcoRI G'AATT_C
Cuts at: 1791 1791
Size: 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4787
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 152 567 1251 1602 1673 2221 2469 3491 4470
Size: 415 684 351 71 548 248 1022 979
Cuts at: 4470 152
Size: 469
Fragments arranged by size:
1022 979 684 548 469 415 351 248
71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2629 2659 2661 2691 2629
Size: 30 2 30 4725
Fragments arranged by size:
4725 30 30 2
HincII GTy'rAC
Cuts at: 942 1826 942
Size: 884 3903
KasI G'GCGC_C
Cuts at: 148 1247 1598 2217 2465 148
Size: 1099 351 619 248 2470
Fragments arranged by size:
2470 1099 619 351 248
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 637 3458 3642 637
Size: 2821 184 1782
Fragments arranged by size:
2821 1782 184
MscI TGG'CCA
Cuts at: 1159 2548 1159
Size: 1389 3398
MslI CAynn'nnrTG
Cuts at: 915 2776 2903 915
Size: 1861 127 2799
Fragments arranged by size:
2799 1861 127
MspA1I CmG'CkG
Cuts at: 146 195 242 378 506 878 1163 1184 1245
Size: 49 47 136 128 372 285 21 61
Cuts at: 1245 1362 1717 1898 2212 2572 3585 3830 146
Size: 117 355 181 314 360 1013 245 1103
Fragments arranged by size:
1103 1013 372 360 355 314 285 245
181 136 128 117 61 49 47 21
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 4787
NarI GG'CG_CC
Cuts at: 149 1248 1599 2218 2466 149
Size: 1099 351 619 248 2470
Fragments arranged by size:
2470 1099 619 351 248
NcoI C'CATG_G
Cuts at: 125 1020 1154 125
Size: 895 134 3758
Fragments arranged by size:
3758 895 134
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 4787
NspI r_CATG'y
Cuts at: 938 1840 2340 3247 938
Size: 902 500 907 2478
Fragments arranged by size:
2478 907 902 500
PasI CC'CwG_GG
Cuts at: 220 1068 1564 220
Size: 848 496 3443
Fragments arranged by size:
3443 848 496
PciI A'CATG_T
Cuts at: 934 3243 934
Size: 2309 2478
PflMI CCAn_nnn'nTGG
Cuts at: 1564 1738 1564
Size: 174 4613
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4755
PpuMI rG'GwC_Cy
Cuts at: 1092 1092
Size: 4787
PstI C_TGCA'G
Cuts at: 1521 1722 1834 2519 1521
Size: 201 112 685 3789
Fragments arranged by size:
3789 685 201 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 506 878 1184 1717 2212 2572 506
Size: 372 306 533 495 360 2721
Fragments arranged by size:
2721 533 495 372 360 306
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 1801 1801
Size: 4787
SacII CC_GC'GG
Cuts at: 379 379
Size: 4787
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 169 759 1225 2810 3020 169
Size: 590 466 1585 210 1936
Fragments arranged by size:
1936 1585 590 466 210
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 4304 4304
Size: 4787
SfcI C'TryA_G
Cuts at: 33 1517 1718 1830 2515 3508 3699 33
Size: 1484 201 112 685 993 191 1121
Fragments arranged by size:
1484 1121 993 685 201 191 112
SfoI GGC'GCC
Cuts at: 150 1249 1600 2219 2467 150
Size: 1099 351 619 248 2470
Fragments arranged by size:
2470 1099 619 351 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1809 1809
Size: 4787
SmlI C'TyrA_G
Cuts at: 1942 2857 3349 3611 1942
Size: 915 492 262 3118
Fragments arranged by size:
3118 915 492 262
SnaBI TAC'GTA
Cuts at: 1779 1779
Size: 4787
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
StuI AGG'CCT
Cuts at: 1483 1483
Size: 4787
StyI C'CwwG_G
Cuts at: 125 134 459 719 1020 1154 1921 2275 125
Size: 9 325 260 301 134 767 354 2637
Fragments arranged by size:
2637 767 354 325 301 260 134 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2370 2503 2962 3171 2370
Size: 133 459 209 3986
Fragments arranged by size:
3986 459 209 133
TatI w'GTAC_w
Cuts at: 4302 4302
Size: 4787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1569 1601 4304 4336 1569
Size: 32 2703 32 2020
Fragments arranged by size:
2703 2020 32 32
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 568 2282 568
Size: 1714 3073
XmaI C'CCGG_G
Cuts at: 1807 1807
Size: 4787
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI BaeI
BbvCI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI