Restriction map for
pF1KSDA0167
(Circular) (Six-base) MAPSORT of: pF1KSDA0167.seq Check: 3649 from: 1 to: 5645
pF1KSDA0167 5645 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1511 1511
Size: 5645
AccI GT'mk_AC
Cuts at: 2633 2683 2633
Size: 50 5595
Acc65I G'GTAC_C
Cuts at: 2661 2661
Size: 5645
AclI AA'CG_TT
Cuts at: 5485 5485
Size: 5645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 1528 1670 1691 3470 3902 4649 4917 537
Size: 991 142 21 1779 432 747 268 1265
Fragments arranged by size:
1779 1265 991 747 432 268 142 21
AflIII A'CryG_T
Cuts at: 2997 4101 5078 2997
Size: 1104 977 3564
Fragments arranged by size:
3564 1104 977
AgeI A'CCGG_T
Cuts at: 4033 4033
Size: 5645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 877 877
Size: 5645
AlwNI CAG_nnn'CTG
Cuts at: 417 538 843 1009 2181 2950 4517 417
Size: 121 305 166 1172 769 1567 1545
Fragments arranged by size:
1567 1545 1172 769 305 166 121
ApaI G_GGCC'C
Cuts at: 1911 1911
Size: 5645
ApaLI G'TGCA_C
Cuts at: 4415 4415
Size: 5645
ApoI r'AATT_y
Cuts at: 1521 1884 2649 4998 5254 1521
Size: 363 765 2349 256 1912
Fragments arranged by size:
2349 1912 765 363 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5645
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5645
AvaI C'yCGr_G
Cuts at: 689 1323 1338 1529 2502 2665 689
Size: 634 15 191 973 163 3669
Fragments arranged by size:
3669 973 634 191 163 15
BamHI G'GATC_C
Cuts at: 2670 2670
Size: 5645
BanI G'GyrC_C
Cuts at: 504 1942 2190 2661 3075 3323 3358 504
Size: 1438 248 471 414 248 35 2791
Fragments arranged by size:
2791 1438 471 414 248 248 35
BanII G_rGCy'C
Cuts at: 720 776 986 1385 1536 1573 1911 2360 2659
Size: 56 210 399 151 37 338 449 299
Cuts at: 2659 3689 720
Size: 1030 2676
Fragments arranged by size:
2676 1030 449 399 338 299 210 151
56 37
BbeI G_GCGC'C
Cuts at: 1946 2194 3079 3327 1946
Size: 248 885 248 4264
Fragments arranged by size:
4264 885 248 248
BbsI GAAGACnn'nnnn_
Cuts at: 430 543 783 430
Size: 113 240 5292
Fragments arranged by size:
5292 240 113
BbvCI CC'TCA_GC
Cuts at: 1365 1537 1365
Size: 172 5473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 982 1016 982
Size: 34 5611
BciVI GTATCCnnnnn_n'
Cuts at: 3543 4304 5645 3543
Size: 761 1341 3543
Fragments arranged by size:
3543 1341 761
BclI T'GATC_A
Cuts at: 293 3165 293
Size: 2872 2773
BfrBI ATG'CAT
Cuts at: 129 129
Size: 5645
BglI GCCn_nnn'nGGC
Cuts at: 196 1861 2245 2455 196
Size: 1665 384 210 3386
Fragments arranged by size:
3386 1665 384 210
BglII A'GATC_T
Cuts at: 3160 5197 3160
Size: 2037 3608
BlpI GC'TnA_GC
Cuts at: 2197 2757 2197
Size: 560 5085
Bme1580I G_kGCm'C
Cuts at: 507 1911 1990 3270 3363 4419 507
Size: 1404 79 1280 93 1056 1733
Fragments arranged by size:
1733 1404 1280 1056 93 79
BmgBI CAC'GTC
Cuts at: 1718 2256 2367 2406 1718
Size: 538 111 39 4957
Fragments arranged by size:
4957 538 111 39
BmrI ACTGGGnnnn_n'
Cuts at: 1247 1457 2857 2938 3273 5417 1247
Size: 210 1400 81 335 2144 1475
Fragments arranged by size:
2144 1475 1400 335 210 81
BmtI G_CTAG'C
Cuts at: 2600 2600
Size: 5645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4012 4012
Size: 5645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 439 580 1580 2473 232
Size: 207 141 1000 893 3404
Fragments arranged by size:
3404 1000 893 207 141
Bpu10I CC'TnA_GC
Cuts at: 1365 1537 1567 1365
Size: 172 30 5443
Fragments arranged by size:
5443 172 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2821 3700 4192 4490 2821
Size: 879 492 298 3976
Fragments arranged by size:
3976 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1556 5349 1556
Size: 3793 1852
BsaAI yAC'GTr
Cuts at: 1922 2637 2998 1922
Size: 715 361 4569
Fragments arranged by size:
4569 715 361
BsaBI GATnn'nnATC
Cuts at: 6 3159 5196 6
Size: 3153 2037 455
Fragments arranged by size:
3153 2037 455
BsaHI Gr'CG_yC
Cuts at: 1943 2191 2420 2615 3076 3324 4026 5522 1943
Size: 248 229 195 461 248 702 1496 2066
Fragments arranged by size:
2066 1496 702 461 248 248 229 195
BsaWI w'CCGG_w
Cuts at: 897 1517 1868 2834 3355 4033 4307 4454 4865
Size: 620 351 966 521 678 274 147 411
Cuts at: 4865 897
Size: 1677
Fragments arranged by size:
1677 966 678 620 521 411 351 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2134 2164 2134
Size: 30 5615
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 647 1632 1759 2155 2224 647
Size: 985 127 396 69 4068
Fragments arranged by size:
4068 985 396 127 69
BseYI C'CCAG_C
Cuts at: 351 499 527 865 1456 1660 2217 2533 2551
Size: 148 28 338 591 204 557 316 18
Cuts at: 2551 2569 3070 3828 4405 4901 351
Size: 18 501 758 577 496 1095
Fragments arranged by size:
1095 758 591 577 557 501 496 338
316 204 148 28 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 2263 1018
Size: 1245 4400
BsiEI CG_ry'CG
Cuts at: 104 2354 3187 3233 4441 104
Size: 2250 833 46 1208 1308
Fragments arranged by size:
2250 1308 1208 833 46
BsiHKAI G_wGCw'C
Cuts at: 720 986 1021 1402 2203 2360 2659 3437 3627
Size: 266 35 381 801 157 299 778 190
Cuts at: 3627 4419 720
Size: 792 1946
Fragments arranged by size:
1946 801 792 778 381 299 266 190
157 35
BsmI GAATG_Cn'
Cuts at: 278 278
Size: 5645
BsmBI CGTCTCn'nnnn_
Cuts at: 598 598
Size: 5645
Bsp1286I G_dGCh'C
Cuts at: 507 720 776 986 1021 1385 1402 1536 1573
Size: 213 56 210 35 364 17 134 37
Cuts at: 1573 1911 1990 2203 2360 2659 3270 3363 3437
Size: 338 79 213 157 299 611 93 74
Cuts at: 3437 3627 3689 4419 507
Size: 190 62 730 1733
Fragments arranged by size:
1733 730 611 364 338 299 213 213
210 190 157 134 93 79 74 62
56 37 35 17
BspEI T'CCGG_A
Cuts at: 1868 4865 1868
Size: 2997 2648
BspHI T'CATG_A
Cuts at: 4821 4821
Size: 5645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1511 2695 2909 3211 3592 1511
Size: 1184 214 302 381 3564
Fragments arranged by size:
3564 1184 381 302 214
BsrBI CCG'CTC
Cuts at: 50 323 1749 2301 3937 3991 5475 5640 50
Size: 273 1426 552 1636 54 1484 165 55
Fragments arranged by size:
1636 1484 1426 552 273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1804 3557 1804
Size: 1753 3892
BsrFI r'CCGG_y
Cuts at: 3643 3824 4033 3643
Size: 181 209 5255
Fragments arranged by size:
5255 209 181
BssSI C'ACGA_G
Cuts at: 707 3916 4274 707
Size: 3209 358 2078
Fragments arranged by size:
3209 2078 358
BstAPI GCAn_nnn'nTGC
Cuts at: 511 2385 3022 511
Size: 1874 637 3134
Fragments arranged by size:
3134 1874 637
BstBI TT'CG_AA
Cuts at: 110 4006 110
Size: 3896 1749
BstEII G'GTnAC_C
Cuts at: 816 973 816
Size: 157 5488
BstYI r'GATC_y
Cuts at: 1 1781 2670 3137 3160 3495 3741 4753 4839
Size: 1780 889 467 23 335 246 1012 86
Cuts at: 4839 4851 5197 1
Size: 12 346 449
Fragments arranged by size:
1780 1012 889 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 2634 2634
Size: 5645
Bsu36I CC'TnA_GG
Cuts at: 211 211
Size: 5645
BtgI C'CryG_G
Cuts at: 597 1281 1601 1850 2032 597
Size: 684 320 249 182 4210
Fragments arranged by size:
4210 684 320 249 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 362 371 3595 3776 3939 362
Size: 9 3224 181 163 2068
Fragments arranged by size:
3224 2068 181 163 9
BtsI GCAGTG_nn'
Cuts at: 164 1010 2888 164
Size: 846 1878 2921
Fragments arranged by size:
2921 1878 846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1877 1912 1877
Size: 35 5610
DraI TTT'AAA
Cuts at: 2644 2644
Size: 5645
DrdI GACnn_nn'nnGTC
Cuts at: 3351 4209 3351
Size: 858 4787
EaeI y'GGCC_r
Cuts at: 188 906 1853 2029 2075 2351 2522 2540 3230
Size: 718 947 176 46 276 171 18 690
Cuts at: 3230 3404 3795 3822 5167 188
Size: 174 391 27 1345 666
Fragments arranged by size:
1345 947 718 690 666 391 276 176
174 171 46 27 18
EagI C'GGCC_G
Cuts at: 2351 3230 2351
Size: 879 4766
EarI CTCTTCn'nnn_
Cuts at: 948 1667 2136 3668 3878 948
Size: 719 469 1532 210 2715
Fragments arranged by size:
2715 1532 719 469 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3853 4163 4309 5455 3853
Size: 310 146 1146 4043
Fragments arranged by size:
4043 1146 310 146
EcoICRI GAG'CTC
Cuts at: 718 984 2358 2657 718
Size: 266 1374 299 3706
Fragments arranged by size:
3706 1374 299 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 439 537 580 1528 1580 1670 1691 2473
Size: 207 98 43 948 52 90 21 782
Cuts at: 2473 3470 3902 4649 4917 232
Size: 997 432 747 268 960
Fragments arranged by size:
997 960 948 782 747 432 268 207
98 90 52 43 21
EcoO109I rG'GnC_Cy
Cuts at: 1028 1907 1908 2319 2784 1028
Size: 879 1 411 465 3889
Fragments arranged by size:
3889 879 465 411 1
EcoRI G'AATT_C
Cuts at: 1884 2649 1884
Size: 765 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 5645
FspI TGC'GCA
Cuts at: 826 1828 3426 826
Size: 1002 1598 3045
Fragments arranged by size:
3045 1598 1002
HaeII r_GCGC'y
Cuts at: 1946 2194 2440 2594 3079 3327 4349 5328 1946
Size: 248 246 154 485 248 1022 979 2263
Fragments arranged by size:
2263 1022 979 485 248 248 246 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 155 187 1477 1509 2134 2166 3487 3517 3519
Size: 32 1290 32 625 32 1321 30 2
Cuts at: 3519 3549 155
Size: 30 2251
Fragments arranged by size:
2251 1321 1290 625 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2684 2684
Size: 5645
HindIII A'AGCT_T
Cuts at: 1101 1101
Size: 5645
KasI G'GCGC_C
Cuts at: 1942 2190 3075 3323 1942
Size: 248 885 248 4264
Fragments arranged by size:
4264 885 248 248
KpnI G_GTAC'C
Cuts at: 2665 2665
Size: 5645
MluI A'CGCG_T
Cuts at: 5078 5078
Size: 5645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4316 4500 4316
Size: 184 5461
MscI TGG'CCA
Cuts at: 190 908 1855 2031 3406 190
Size: 718 947 176 1375 2429
Fragments arranged by size:
2429 1375 947 718 176
MslI CAynn'nnrTG
Cuts at: 3634 3761 3634
Size: 127 5518
MspA1I CmG'CkG
Cuts at: 606 886 1000 2196 2217 2229 2328 2469 2756
Size: 280 114 1196 21 12 99 141 287
Cuts at: 2756 3070 3430 4443 4688 606
Size: 314 360 1013 245 1563
Fragments arranged by size:
1563 1196 1013 360 314 287 280 245
141 114 99 21 12
NaeI GCC'GGC
Cuts at: 3826 3826
Size: 5645
NarI GG'CG_CC
Cuts at: 1943 2191 3076 3324 1943
Size: 248 885 248 4264
Fragments arranged by size:
4264 885 248 248
NcoI C'CATG_G
Cuts at: 597 1601 597
Size: 1004 4641
NgoMIV G'CCGG_C
Cuts at: 3824 3824
Size: 5645
NheI G'CTAG_C
Cuts at: 2596 2596
Size: 5645
NsiI A_TGCA'T
Cuts at: 131 131
Size: 5645
NspI r_CATG'y
Cuts at: 133 2698 3198 4105 133
Size: 2565 500 907 1673
Fragments arranged by size:
2565 1673 907 500
PasI CC'CwG_GG
Cuts at: 1024 2424 1024
Size: 1400 4245
PciI A'CATG_T
Cuts at: 4101 4101
Size: 5645
PflMI CCAn_nnn'nTGG
Cuts at: 417 801 813 2256 417
Size: 384 12 1443 3806
Fragments arranged by size:
3806 1443 384 12
PfoI T'CCnGG_A
Cuts at: 248 794 248
Size: 546 5099
PmeI GTTT'AAAC
Cuts at: 2644 2644
Size: 5645
PmlI CAC'GTG
Cuts at: 1922 1922
Size: 5645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4784 4816 4784
Size: 32 5613
PpuMI rG'GwC_Cy
Cuts at: 1028 2319 1028
Size: 1291 4354
PspOMI G'GGCC_C
Cuts at: 1907 1907
Size: 5645
PstI C_TGCA'G
Cuts at: 159 745 1005 1475 2692 3377 159
Size: 586 260 470 1217 685 2427
Fragments arranged by size:
2427 1217 685 586 470 260
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5645
PvuII CAG'CTG
Cuts at: 606 886 1000 2229 2328 2469 3070 3430 606
Size: 280 114 1229 99 141 601 360 2821
Fragments arranged by size:
2821 1229 601 360 280 141 114 99
RsrII CG'GwC_CG
Cuts at: 3840 3840
Size: 5645
SacI G_AGCT'C
Cuts at: 720 986 2360 2659 720
Size: 266 1374 299 3706
Fragments arranged by size:
3706 1374 299 266
SalI G'TCGA_C
Cuts at: 2682 2682
Size: 5645
SanDI GG'GwC_CC
Cuts at: 1028 1028
Size: 5645
SapI GCTCTTCn'nnn_
Cuts at: 3668 3878 3668
Size: 210 5435
SbfI CC_TGCA'GG
Cuts at: 2692 2692
Size: 5645
ScaI AGT'ACT
Cuts at: 5162 5162
Size: 5645
SexAI A'CCwGG_T
Cuts at: 410 410
Size: 5645
SfcI C'TryA_G
Cuts at: 33 155 741 1001 1471 2688 3373 4366 4557
Size: 122 586 260 470 1217 685 993 191
Cuts at: 4557 33
Size: 1121
Fragments arranged by size:
1217 1121 993 685 586 470 260 191
122
SfoI GGC'GCC
Cuts at: 1944 2192 3077 3325 1944
Size: 248 885 248 4264
Fragments arranged by size:
4264 885 248 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5645
SmaI CCC'GGG
Cuts at: 1325 2504 2667 1325
Size: 1179 163 4303
Fragments arranged by size:
4303 1179 163
SmlI C'TyrA_G
Cuts at: 2800 3715 4207 4469 2800
Size: 915 492 262 3976
Fragments arranged by size:
3976 915 492 262
SnaBI TAC'GTA
Cuts at: 2637 2637
Size: 5645
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5645
SphI G_CATG'C
Cuts at: 133 2698 3198 133
Size: 2565 500 2580
Fragments arranged by size:
2580 2565 500
StuI AGG'CCT
Cuts at: 265 638 1421 265
Size: 373 783 4489
Fragments arranged by size:
4489 783 373
StyI C'CwwG_G
Cuts at: 597 644 1601 1877 2779 3133 597
Size: 47 957 276 902 354 3109
Fragments arranged by size:
3109 957 902 354 276 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 1402 3228 3361 3820 4029 334
Size: 1068 1826 133 459 209 1950
Fragments arranged by size:
1950 1826 1068 459 209 133
TatI w'GTAC_w
Cuts at: 440 5160 440
Size: 4720 925
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5162 5194 5162
Size: 32 5613
Tth111I GACn'n_nGTC
Cuts at: 3442 3442
Size: 5645
XbaI T'CTAG_A
Cuts at: 2676 2676
Size: 5645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1935 3140 1935
Size: 1205 4440
XmaI C'CCGG_G
Cuts at: 1323 2502 2665 1323
Size: 1179 163 4303
Fragments arranged by size:
4303 1179 163
XmnI GAAnn'nnTTC
Cuts at: 574 574
Size: 5645
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BsiWI BsrGI BssHII BstXI ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI
PacI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SrfI SspI SwaI XhoI ZraI