Restriction map for
pF1KSDA0168
(Circular) (Six-base) MAPSORT of: pF1KSDA0168.seq Check: 2856 from: 1 to: 4115
pF1KSDA0168 4115 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 442 535 760 850 1940 2372 3119 3387
Size: 216 93 225 90 1090 432 747 268
Cuts at: 3387 226
Size: 954
Fragments arranged by size:
1090 954 747 432 268 225 216 93
90
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 738 828 1071 1420 2987 738
Size: 90 243 349 1567 1866
Fragments arranged by size:
1866 1567 349 243 90
ApaI G_GGCC'C
Cuts at: 874 874
Size: 4115
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 237 757 771 1119 3468 3724 237
Size: 520 14 348 2349 256 628
Fragments arranged by size:
2349 628 520 348 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 1135 1135
Size: 4115
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 251 1131 1545 1793 1828 251
Size: 880 414 248 35 2538
Fragments arranged by size:
2538 880 414 248 35
BanII G_rGCy'C
Cuts at: 290 408 874 1129 2159 290
Size: 118 466 255 1030 2246
Fragments arranged by size:
2246 1030 466 255 118
BbeI G_GCGC'C
Cuts at: 1549 1797 1549
Size: 248 3867
BbsI GAAGACnn'nnnn_
Cuts at: 214 272 214
Size: 58 4057
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglII A'GATC_T
Cuts at: 449 884 1630 3667 449
Size: 435 746 2037 897
Fragments arranged by size:
2037 897 746 435
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 440 874 1740 1833 2889 440
Size: 434 866 93 1056 1666
Fragments arranged by size:
1666 1056 866 434 93
BmgBI CAC'GTC
Cuts at: 939 939
Size: 4115
BmrI ACTGGGnnnn_n'
Cuts at: 939 1327 1408 1743 3887 939
Size: 388 81 335 2144 1167
Fragments arranged by size:
2144 1167 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 903 2482 903
Size: 1579 2536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 850 979 1093 489
Size: 361 129 114 3511
Fragments arranged by size:
3511 361 129 114
Bpu10I CC'TnA_GC
Cuts at: 407 407
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2170 2662 2960 1291
Size: 879 492 298 2446
Fragments arranged by size:
2446 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1081 3819 1081
Size: 2738 1377
BsaAI yAC'GTr
Cuts at: 798 1107 1468 798
Size: 309 361 3445
Fragments arranged by size:
3445 361 309
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 595 621 1546 1794 2496 3992 595
Size: 26 925 248 702 1496 718
Fragments arranged by size:
1496 925 718 702 248 26
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2777 2924 3335 1304
Size: 521 678 274 147 411 2084
Fragments arranged by size:
2084 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 616 646 616
Size: 30 4085
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 363 366 796 1090 363
Size: 3 430 294 3388
Fragments arranged by size:
3388 430 294 3
BseYI C'CCAG_C
Cuts at: 1092 1540 2298 2875 3371 1092
Size: 448 758 577 496 1836
Fragments arranged by size:
1836 758 577 496 448
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1049 1129 1907 2097 2889 1049
Size: 80 778 190 792 2275
Fragments arranged by size:
2275 792 778 190 80
BsmI GAATG_Cn'
Cuts at: 353 353
Size: 4115
Bsp1286I G_dGCh'C
Cuts at: 290 408 440 874 1049 1129 1740 1833 1907
Size: 118 32 434 175 80 611 93 74
Cuts at: 1907 2097 2159 2889 290
Size: 190 62 730 1516
Fragments arranged by size:
1516 730 611 434 190 175 118 93
80 74 62 32
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1165 1379 1681 2062 1165
Size: 214 302 381 3218
Fragments arranged by size:
3218 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 591 2027 591
Size: 1436 2679
BsrFI r'CCGG_y
Cuts at: 314 966 2113 2294 2503 314
Size: 652 1147 181 209 1926
Fragments arranged by size:
1926 1147 652 209 181
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 110 2476 110
Size: 2366 1749
BstEII G'GTnAC_C
Cuts at: 478 478
Size: 4115
BstXI CCAn_nnnn'nTGG
Cuts at: 811 811
Size: 4115
BstYI r'GATC_y
Cuts at: 1 449 884 1140 1607 1630 1965 2211 3223
Size: 448 435 256 467 23 335 246 1012
Cuts at: 3223 3309 3321 3667 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 448 435 346 335 256
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 579 125
Size: 454 3661
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2065 2246 2409 2065
Size: 181 163 3771
Fragments arranged by size:
3771 181 163
BtsI GCAGTG_nn'
Cuts at: 564 1358 564
Size: 794 3321
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DraIII CAC_nnn'GTG
Cuts at: 559 559
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 642 1700 1874 2265 2292 3637 642
Size: 1058 174 391 27 1345 1120
Fragments arranged by size:
1345 1120 1058 391 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1127 1127
Size: 4115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 442 489 535 760 850 979 1093 1940
Size: 216 47 46 225 90 129 114 847
Cuts at: 1940 2372 3119 3387 226
Size: 432 747 268 954
Fragments arranged by size:
954 847 747 432 268 225 216 129
114 90 47 46
EcoNI CCTnn'n_nnAGG
Cuts at: 526 526
Size: 4115
EcoO109I rG'GnC_Cy
Cuts at: 306 510 735 870 1254 306
Size: 204 225 135 384 3167
Fragments arranged by size:
3167 384 225 204 135
EcoRI G'AATT_C
Cuts at: 771 1119 771
Size: 348 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 550 1033 1896 550
Size: 483 863 2769
Fragments arranged by size:
2769 863 483
FspAI rTGC'GCAy
Cuts at: 550 550
Size: 4115
HaeII r_GCGC'y
Cuts at: 1549 1797 2819 3798 1549
Size: 248 1022 979 1866
Fragments arranged by size:
1866 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1957 1987 1989 2019 1957
Size: 30 2 30 4053
Fragments arranged by size:
4053 30 30 2
HincII GTy'rAC
Cuts at: 1154 1154
Size: 4115
HindIII A'AGCT_T
Cuts at: 978 978
Size: 4115
KasI G'GCGC_C
Cuts at: 1545 1793 1545
Size: 248 3867
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2786 2970 2786
Size: 184 3931
MscI TGG'CCA
Cuts at: 1876 1876
Size: 4115
MslI CAynn'nnrTG
Cuts at: 809 2104 2231 809
Size: 1295 127 2693
Fragments arranged by size:
2693 1295 127
MspA1I CmG'CkG
Cuts at: 543 609 849 1226 1540 1900 2913 3158 543
Size: 66 240 377 314 360 1013 245 1500
Fragments arranged by size:
1500 1013 377 360 314 245 240 66
NaeI GCC'GGC
Cuts at: 316 2296 316
Size: 1980 2135
NarI GG'CG_CC
Cuts at: 1546 1794 1546
Size: 248 3867
NcoI C'CATG_G
Cuts at: 125 125
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 314 2294 314
Size: 1980 2135
NspI r_CATG'y
Cuts at: 1168 1668 2575 1168
Size: 500 907 2708
Fragments arranged by size:
2708 907 500
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PflMI CCAn_nnn'nTGG
Cuts at: 685 685
Size: 4115
PfoI T'CCnGG_A
Cuts at: 995 995
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 4115
PspOMI G'GGCC_C
Cuts at: 870 870
Size: 4115
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 940 972 940
Size: 32 4083
PstI C_TGCA'G
Cuts at: 332 527 1162 1847 332
Size: 195 635 685 2600
Fragments arranged by size:
2600 685 635 195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 543 1540 1900 543
Size: 997 360 2758
Fragments arranged by size:
2758 997 360
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 1129 1129
Size: 4115
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 527 1162 527
Size: 635 3480
ScaI AGT'ACT
Cuts at: 3632 3632
Size: 4115
SexAI A'CCwGG_T
Cuts at: 914 914
Size: 4115
SfcI C'TryA_G
Cuts at: 33 132 328 523 1158 1843 2836 3027 33
Size: 99 196 195 635 685 993 191 1121
Fragments arranged by size:
1121 993 685 635 196 195 191 99
SfoI GGC'GCC
Cuts at: 1547 1795 1547
Size: 248 3867
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 1270 2185 2677 2939 1270
Size: 915 492 262 2446
Fragments arranged by size:
2446 915 492 262
SnaBI TAC'GTA
Cuts at: 1107 1107
Size: 4115
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StyI C'CwwG_G
Cuts at: 125 1249 1603 125
Size: 1124 354 2637
Fragments arranged by size:
2637 1124 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1698 1831 2290 2499 1698
Size: 133 459 209 3314
Fragments arranged by size:
3314 459 209 133
TatI w'GTAC_w
Cuts at: 221 1037 3630 221
Size: 816 2593 706
Fragments arranged by size:
2593 816 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 162 585 616 617 648 3632 3664 130
Size: 32 423 31 1 31 2984 32 581
Fragments arranged by size:
2984 581 423 32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 1610
Size: 4115
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
XmnI GAAnn'nnTTC
Cuts at: 353 353
Size: 4115
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI
XhoI ZraI