Restriction map for
pF1KSDA0170
(Circular) (Six-base) MAPSORT of: pF1KSDA0170.seq Check: 8129 from: 1 to: 9404
pF1KSDA0170 9404 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3171 3171
Size: 9404
AccI GT'mk_AC
Cuts at: 2571 6442 2571
Size: 3871 5533
Acc65I G'GTAC_C
Cuts at: 2347 6420 2347
Size: 4073 5331
AclI AA'CG_TT
Cuts at: 9244 9244
Size: 9404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 896 2198 2219 3275 4843 6355 7229 7661
Size: 6 1302 21 1056 1568 1512 874 432
Cuts at: 7661 8408 8676 890
Size: 747 268 1618
Fragments arranged by size:
1618 1568 1512 1302 1056 874 747 432
268 21 6
AfeI AGC'GCT
Cuts at: 1364 1364
Size: 9404
AflII C'TTAA_G
Cuts at: 1969 1969
Size: 9404
AflIII A'CryG_T
Cuts at: 1437 2154 6756 7860 8837 1437
Size: 717 4602 1104 977 2004
Fragments arranged by size:
4602 2004 1104 977 717
AgeI A'CCGG_T
Cuts at: 7792 7792
Size: 9404
AleI CACnn'nnGTG
Cuts at: 787 1030 1492 1678 1696 5821 787
Size: 243 462 186 18 4125 4370
Fragments arranged by size:
4370 4125 462 243 186 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3839 3871 5302 5334 3593
Size: 32 214 32 1431 32 7663
Fragments arranged by size:
7663 1431 214 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2247 3031 3253 3622 3868 3991 4114 4483 4606
Size: 784 222 369 246 123 123 369 123
Cuts at: 4606 4729 4975 5098 6709 8276 2247
Size: 123 246 123 1611 1567 3375
Fragments arranged by size:
3375 1611 1567 784 369 369 246 246
222 123 123 123 123 123
ApaI G_GGCC'C
Cuts at: 556 2001 2602 556
Size: 1445 601 7358
Fragments arranged by size:
7358 1445 601
ApaLI G'TGCA_C
Cuts at: 8174 8174
Size: 9404
ApoI r'AATT_y
Cuts at: 5221 6408 8757 9013 5221
Size: 1187 2349 256 5612
Fragments arranged by size:
5612 2349 1187 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9404
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9404
AvaI C'yCGr_G
Cuts at: 277 548 4292 4366 4784 4907 5834 6095 6424
Size: 271 3744 74 418 123 927 261 329
Cuts at: 6424 277
Size: 3257
Fragments arranged by size:
3744 3257 927 418 329 271 261 123
74
AvrII C'CTAG_G
Cuts at: 2470 2470
Size: 9404
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 929 962 1247 1280 929
Size: 33 285 33 9053
Fragments arranged by size:
9053 285 33 33
BamHI G'GATC_C
Cuts at: 1136 1646 3106 6429 1136
Size: 510 1460 3323 4111
Fragments arranged by size:
4111 3323 1460 510
BanI G'GyrC_C
Cuts at: 241 381 844 1876 2347 2934 6420 6834 7082
Size: 140 463 1032 471 587 3486 414 248
Cuts at: 7082 7117 241
Size: 35 2528
Fragments arranged by size:
3486 2528 1032 587 471 463 414 248
140 35
BanII G_rGCy'C
Cuts at: 451 556 1089 1511 1563 1639 1986 2001 2124
Size: 105 533 422 52 76 347 15 123
Cuts at: 2124 2506 2602 3121 3506 3629 3671 3752 3875
Size: 382 96 519 385 123 42 81 123
Cuts at: 3875 3917 3998 4121 4244 4286 4409 4490 4655
Size: 42 81 123 123 42 123 81 165
Cuts at: 4655 4736 4778 4901 4982 5534 5744 6096 6418
Size: 81 42 123 81 552 210 352 322
Cuts at: 6418 7448 451
Size: 1030 2407
Fragments arranged by size:
2407 1030 552 533 519 422 385 382
352 347 322 210 165 123 123 123
123 123 123 123 105 96 81 81
81 81 81 76 52 42 42 42
42 15
BbeI G_GCGC'C
Cuts at: 6838 7086 6838
Size: 248 9156
BbsI GAAGACnn'nnnn_
Cuts at: 2186 4459 5174 5635 2186
Size: 2273 715 461 5955
Fragments arranged by size:
5955 2273 715 461
BbvCI CC'TCA_GC
Cuts at: 620 828 1067 3066 620
Size: 208 239 1999 6958
Fragments arranged by size:
6958 1999 239 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1102 1136 1102
Size: 34 9370
BciVI GTATCCnnnnn_n'
Cuts at: 7302 8063 9404 7302
Size: 761 1341 7302
Fragments arranged by size:
7302 1341 761
BclI T'GATC_A
Cuts at: 6242 6924 6242
Size: 682 8722
BfrBI ATG'CAT
Cuts at: 1734 1734
Size: 9404
BglI GCCn_nnn'nGGC
Cuts at: 219 1285 219
Size: 1066 8338
BglII A'GATC_T
Cuts at: 1178 2064 2202 5512 6125 6919 8956 1178
Size: 886 138 3310 613 794 2037 1626
Fragments arranged by size:
3310 2037 1626 886 794 613 138
BlpI GC'TnA_GC
Cuts at: 1088 6516 1088
Size: 5428 3976
Bme1580I G_kGCm'C
Cuts at: 246 556 1879 2001 2602 2939 3737 3983 4721
Size: 310 1323 122 601 337 798 246 738
Cuts at: 4721 5946 7029 7122 8178 246
Size: 1225 1083 93 1056 1472
Fragments arranged by size:
1472 1323 1225 1083 1056 798 738 601
337 310 246 122 93
BmgBI CAC'GTC
Cuts at: 1494 1494
Size: 9404
BmrI ACTGGGnnnn_n'
Cuts at: 159 1144 1507 2217 2500 3106 5273 5869 6616
Size: 985 363 710 283 606 2167 596 747
Cuts at: 6616 6697 7032 9176 159
Size: 81 335 2144 387
Fragments arranged by size:
2167 2144 985 747 710 606 596 387
363 335 283 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 840 843 1022 1896 2074 5463 6110 7771 840
Size: 3 179 874 178 3389 647 1661 2473
Fragments arranged by size:
3389 2473 1661 874 647 179 178 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1031 1229 1318 1675 2254 2971 2997 3209
Size: 19 198 89 357 579 717 26 212
Cuts at: 3209 3377 3439 3447 3613 3736 3859 4105 4474
Size: 168 62 8 166 123 123 246 369
Cuts at: 4474 4720 4857 5518 6161 6347 1012
Size: 246 137 661 643 186 4069
Fragments arranged by size:
4069 717 661 643 579 369 357 246
246 212 198 186 168 166 137 123
123 89 62 26 19 8
Bpu10I CC'TnA_GC
Cuts at: 620 828 1067 3066 3500 3623 3869 3992 4115
Size: 208 239 1999 434 123 246 123 123
Cuts at: 4115 4157 4238 4280 4361 4403 4484 4526 4607
Size: 42 81 42 81 42 81 42 81
Cuts at: 4607 4730 4772 4853 4895 4952 4976 5153 5261
Size: 123 42 81 42 57 24 177 108
Cuts at: 5261 5438 6044 620
Size: 177 606 3980
Fragments arranged by size:
3980 1999 606 434 246 239 208 177
177 123 123 123 123 108 81 81
81 81 81 57 42 42 42 42
42 42 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 1522 2031 2461 3766 5125 5367 5482 6580
Size: 1306 509 430 1305 1359 242 115 1098
Cuts at: 6580 7459 7951 8249 216
Size: 879 492 298 1371
Fragments arranged by size:
1371 1359 1306 1305 1098 879 509 492
430 298 242 115
BsaI GGTCTCn'nnnn_
Cuts at: 423 2410 2480 2880 5717 9108 423
Size: 1987 70 400 2837 3391 719
Fragments arranged by size:
3391 2837 1987 719 400 70
BsaAI yAC'GTr
Cuts at: 6396 6757 6396
Size: 361 9043
BsaBI GATnn'nnATC
Cuts at: 6 361 6918 8955 6
Size: 355 6557 2037 455
Fragments arranged by size:
6557 2037 455 355
BsaHI Gr'CG_yC
Cuts at: 6835 7083 7785 9281 6835
Size: 248 702 1496 6958
Fragments arranged by size:
6958 1496 702 248
BsaWI w'CCGG_w
Cuts at: 6593 7114 7792 8066 8213 8624 6593
Size: 521 678 274 147 411 7373
Fragments arranged by size:
7373 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 374 404 2111 2141 5302 5332 374
Size: 30 1707 30 3161 30 4446
Fragments arranged by size:
4446 3161 1707 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 184 646 816 832 1011 1513 1885 5749
Size: 3 462 170 16 179 502 372 3864
Cuts at: 5749 5752 6292 181
Size: 3 540 3293
Fragments arranged by size:
3864 3293 540 502 462 372 179 170
16 3 3
BseYI C'CCAG_C
Cuts at: 979 1140 1942 2102 2420 3261 3527 5305 5453
Size: 161 802 160 318 841 266 1778 148
Cuts at: 5453 6205 6829 7587 8164 8660 979
Size: 752 624 758 577 496 1723
Fragments arranged by size:
1778 1723 841 802 758 752 624 577
496 318 266 161 160 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 4941 5339 977
Size: 3964 398 5042
Fragments arranged by size:
5042 3964 398
BsiEI CG_ry'CG
Cuts at: 104 3774 6946 6992 8200 104
Size: 3670 3172 46 1208 1308
Fragments arranged by size:
3670 3172 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1215 1511 1575 1639 1986 3629 3752 3875 3998
Size: 296 64 64 347 1643 123 123 123
Cuts at: 3998 4121 4244 4490 4736 5534 5813 6418 7196
Size: 123 123 246 246 798 279 605 778
Cuts at: 7196 7386 8178 1215
Size: 190 792 2441
Fragments arranged by size:
2441 1643 798 792 778 605 347 296
279 246 246 190 123 123 123 123
123 64 64
BsmI GAATG_Cn'
Cuts at: 305 726 305
Size: 421 8983
BsmBI CGTCTCn'nnnn_
Cuts at: 4541 4541
Size: 9404
Bsp1286I G_dGCh'C
Cuts at: 246 451 556 1089 1215 1511 1563 1575 1639
Size: 205 105 533 126 296 52 12 64
Cuts at: 1639 1879 1986 2001 2124 2506 2602 2939 3121
Size: 240 107 15 123 382 96 337 182
Cuts at: 3121 3506 3629 3671 3737 3752 3875 3917 3983
Size: 385 123 42 66 15 123 42 66
Cuts at: 3983 3998 4121 4244 4286 4409 4490 4655 4721
Size: 15 123 123 42 123 81 165 66
Cuts at: 4721 4736 4778 4901 4982 5534 5744 5813 5946
Size: 15 42 123 81 552 210 69 133
Cuts at: 5946 6096 6418 7029 7122 7196 7386 7448 8178
Size: 150 322 611 93 74 190 62 730
Cuts at: 8178 246
Size: 1472
Fragments arranged by size:
1472 730 611 552 533 385 382 337
322 296 240 210 205 190 182 165
150 133 126 123 123 123 123 123
123 123 107 105 96 93 81 81
74 69 66 66 66 64 62 52
42 42 42 42 15 15 15 15
12
BspEI T'CCGG_A
Cuts at: 8624 8624
Size: 9404
BspHI T'CATG_A
Cuts at: 1986 8580 1986
Size: 6594 2810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3171 5497 6454 6668 6970 7351 3171
Size: 2326 957 214 302 381 5224
Fragments arranged by size:
5224 2326 957 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5844 7696 7750 9234 9399 50
Size: 5794 1852 54 1484 165 55
Fragments arranged by size:
5794 1852 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2325 6267 7316 2325
Size: 3942 1049 4413
Fragments arranged by size:
4413 3942 1049
BsrFI r'CCGG_y
Cuts at: 5202 7402 7583 7792 5202
Size: 2200 181 209 6814
Fragments arranged by size:
6814 2200 209 181
BssSI C'ACGA_G
Cuts at: 7675 8033 7675
Size: 358 9046
BstAPI GCAn_nnn'nTGC
Cuts at: 6781 6781
Size: 9404
BstBI TT'CG_AA
Cuts at: 110 7765 110
Size: 7655 1749
BstEII G'GTnAC_C
Cuts at: 6033 6033
Size: 9404
BstXI CCAn_nnnn'nTGG
Cuts at: 1009 1680 2314 3097 1009
Size: 671 634 783 7316
Fragments arranged by size:
7316 783 671 634
BstYI r'GATC_y
Cuts at: 1 1136 1178 1646 2064 2202 3106 3703 3826
Size: 1135 42 468 418 138 904 597 123
Cuts at: 3826 3949 4318 4564 5486 5512 6125 6429 6896
Size: 123 369 246 922 26 613 304 467
Cuts at: 6896 6919 7254 7500 8512 8598 8610 8956 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1135 1012 922 904 613 597 468 467
449 418 369 346 335 304 246 246
138 123 123 86 42 26 23 12
BstZ17I GTA'TAC
Cuts at: 2572 2572
Size: 9404
Bsu36I CC'TnA_GG
Cuts at: 437 5141 5540 6254 437
Size: 4704 399 714 3587
Fragments arranged by size:
4704 3587 714 399
BtgI C'CryG_G
Cuts at: 125 246 1185 1316 2362 5576 125
Size: 121 939 131 1046 3214 3953
Fragments arranged by size:
3953 3214 1046 939 131 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 502 7354 7535 7698 502
Size: 6852 181 163 2208
Fragments arranged by size:
6852 2208 181 163
BtsI GCAGTG_nn'
Cuts at: 5411 6647 5411
Size: 1236 8168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3726 3761 3726
Size: 35 9369
DraI TTT'AAA
Cuts at: 6403 6403
Size: 9404
DraIII CAC_nnn'GTG
Cuts at: 4919 4919
Size: 9404
DrdI GACnn_nn'nnGTC
Cuts at: 3579 3655 3778 3901 4024 4071 4147 4194 4393
Size: 76 123 123 123 47 76 47 199
Cuts at: 4393 4440 4639 4762 4809 4932 5008 5055 5131
Size: 47 199 123 47 123 76 47 76
Cuts at: 5131 7110 7968 3579
Size: 1979 858 5015
Fragments arranged by size:
5015 1979 858 199 199 123 123 123
123 123 76 76 76 76 47 47
47 47 47
EaeI y'GGCC_r
Cuts at: 6989 7163 7554 7581 8926 6989
Size: 174 391 27 1345 7467
Fragments arranged by size:
7467 1345 391 174 27
EagI C'GGCC_G
Cuts at: 6989 6989
Size: 9404
EarI CTCTTCn'nnn_
Cuts at: 158 563 713 834 1124 1165 1415 1499 2921
Size: 405 150 121 290 41 250 84 1422
Cuts at: 2921 5642 5678 5818 7427 7637 158
Size: 2721 36 140 1609 210 1925
Fragments arranged by size:
2721 1925 1609 1422 405 290 250 210
150 140 121 84 41 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3305 4611 5908 7612 7922 8068 9214 3305
Size: 1306 1297 1704 310 146 1146 3495
Fragments arranged by size:
3495 1704 1306 1297 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1509 1637 1984 3627 3750 3873 3996 4119 4242
Size: 128 347 1643 123 123 123 123 123
Cuts at: 4242 4488 4734 5532 6416 1509
Size: 246 246 798 884 4497
Fragments arranged by size:
4497 1643 884 798 347 246 246 128
123 123 123 123 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 896 1012 1031 1229 1318 1675 2198 2219
Size: 6 116 19 198 89 357 523 21
Cuts at: 2219 2254 2971 2997 3209 3275 3377 3439 3447
Size: 35 717 26 212 66 102 62 8
Cuts at: 3447 3613 3736 3859 4105 4474 4720 4843 4857
Size: 166 123 123 246 369 246 123 14
Cuts at: 4857 5518 6161 6347 6355 7229 7661 8408 8676
Size: 661 643 186 8 874 432 747 268
Cuts at: 8676 890
Size: 1618
Fragments arranged by size:
1618 874 747 717 661 643 523 432
369 357 268 246 246 212 198 186
166 123 123 123 116 102 89 66
62 35 26 21 19 14 8 8
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2606 2606
Size: 9404
EcoO109I rG'GnC_Cy
Cuts at: 552 553 1204 1278 1997 2034 2127 2244 2370
Size: 1 651 74 719 37 93 117 126
Cuts at: 2370 2599 3048 3058 3581 4442 4811 4934 5057
Size: 229 449 10 523 861 369 123 123
Cuts at: 5057 6543 552
Size: 1486 3413
Fragments arranged by size:
3413 1486 861 719 651 523 449 369
229 126 123 123 117 93 74 37
10 1
EcoRI G'AATT_C
Cuts at: 5221 6408 5221
Size: 1187 8217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1328 7423 1328
Size: 6095 3309
FspI TGC'GCA
Cuts at: 7185 7185
Size: 9404
HaeII r_GCGC'y
Cuts at: 1366 6838 7086 8108 9087 1366
Size: 5472 248 1022 979 1683
Fragments arranged by size:
5472 1683 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 450 482 1517 1549 1621 1653 2111 2143 3205
Size: 32 1035 32 72 32 458 32 1062
Cuts at: 3205 3237 3658 3690 3695 3727 3818 3850 3941
Size: 32 421 32 5 32 91 32 91
Cuts at: 3941 3973 4273 4305 4396 4428 4519 4551 4552
Size: 32 300 32 91 32 91 32 1
Cuts at: 4552 4556 4584 4588 4642 4674 4765 4797 4888
Size: 4 28 4 54 32 91 32 91
Cuts at: 4888 4920 5603 5635 6227 6259 6370 6402 7246
Size: 32 683 32 592 32 111 32 844
Cuts at: 7246 7276 7278 7308 450
Size: 30 2 30 2546
Fragments arranged by size:
2546 1062 1035 844 683 592 458 421
300 111 91 91 91 91 91 91
72 54 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 28 5
4 4 2 1
HincII GTy'rAC
Cuts at: 2297 6443 2297
Size: 4146 5258
HindIII A'AGCT_T
Cuts at: 1310 1310
Size: 9404
KasI G'GCGC_C
Cuts at: 6834 7082 6834
Size: 248 9156
KpnI G_GTAC'C
Cuts at: 2351 6424 2351
Size: 4073 5331
MfeI C'AATT_G
Cuts at: 4835 4835
Size: 9404
MluI A'CGCG_T
Cuts at: 8837 8837
Size: 9404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5793 8075 8259 5793
Size: 2282 184 6938
Fragments arranged by size:
6938 2282 184
MscI TGG'CCA
Cuts at: 7165 7165
Size: 9404
MslI CAynn'nnrTG
Cuts at: 787 1030 1162 1492 1678 1696 2159 5821 7393
Size: 243 132 330 186 18 463 3662 1572
Cuts at: 7393 7520 787
Size: 127 2671
Fragments arranged by size:
3662 2671 1572 463 330 243 186 132
127 18
MspA1I CmG'CkG
Cuts at: 832 979 1066 1963 5305 5416 5884 6515 6829
Size: 147 87 897 3342 111 468 631 314
Cuts at: 6829 7189 8202 8447 832
Size: 360 1013 245 1789
Fragments arranged by size:
3342 1789 1013 897 631 468 360 314
245 147 111 87
NaeI GCC'GGC
Cuts at: 7585 7585
Size: 9404
NarI GG'CG_CC
Cuts at: 6835 7083 6835
Size: 248 9156
NcoI C'CATG_G
Cuts at: 125 246 1185 1316 2362 5576 125
Size: 121 939 131 1046 3214 3953
Fragments arranged by size:
3953 3214 1046 939 131 121
NgoMIV G'CCGG_C
Cuts at: 7583 7583
Size: 9404
NsiI A_TGCA'T
Cuts at: 1736 1736
Size: 9404
NspI r_CATG'y
Cuts at: 352 1431 2158 6213 6251 6457 6957 7864 352
Size: 1079 727 4055 38 206 500 907 1892
Fragments arranged by size:
4055 1892 1079 907 727 500 206 38
PasI CC'CwG_GG
Cuts at: 451 2001 5946 451
Size: 1550 3945 3909
Fragments arranged by size:
3945 3909 1550
PciI A'CATG_T
Cuts at: 2154 7860 2154
Size: 5706 3698
PflMI CCAn_nnn'nTGG
Cuts at: 522 1440 2265 3031 5496 522
Size: 918 825 766 2465 4430
Fragments arranged by size:
4430 2465 918 825 766
PfoI T'CCnGG_A
Cuts at: 1008 1053 1206 2948 3054 3178 1008
Size: 45 153 1742 106 124 7234
Fragments arranged by size:
7234 1742 153 124 106 45
PmeI GTTT'AAAC
Cuts at: 6403 6403
Size: 9404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5302 5334 8543 8575 5302
Size: 32 3209 32 6131
Fragments arranged by size:
6131 3209 32 32
PpuMI rG'GwC_Cy
Cuts at: 1204 2034 2127 2370 3048 3058 3581 4442 4811
Size: 830 93 243 678 10 523 861 369
Cuts at: 4811 4934 5057 1204
Size: 123 123 5551
Fragments arranged by size:
5551 861 830 678 523 369 243 123
123 93 10
PspOMI G'GGCC_C
Cuts at: 552 1997 2598 552
Size: 1445 601 7358
Fragments arranged by size:
7358 1445 601
PspXI vC'TCGA_Gb
Cuts at: 4366 4366
Size: 9404
PstI C_TGCA'G
Cuts at: 1235 1454 1962 3249 5667 6451 7136 1235
Size: 219 508 1287 2418 784 685 3503
Fragments arranged by size:
3503 2418 1287 784 685 508 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9404
PvuII CAG'CTG
Cuts at: 832 979 1066 1963 5416 6829 7189 832
Size: 147 87 897 3453 1413 360 3047
Fragments arranged by size:
3453 3047 1413 897 360 147 87
RsrII CG'GwC_CG
Cuts at: 7599 7599
Size: 9404
SacI G_AGCT'C
Cuts at: 1511 1639 1986 3629 3752 3875 3998 4121 4244
Size: 128 347 1643 123 123 123 123 123
Cuts at: 4244 4490 4736 5534 6418 1511
Size: 246 246 798 884 4497
Fragments arranged by size:
4497 1643 884 798 347 246 246 128
123 123 123 123 123
SalI G'TCGA_C
Cuts at: 6441 6441
Size: 9404
SapI GCTCTTCn'nnn_
Cuts at: 1499 5818 7427 7637 1499
Size: 4319 1609 210 3266
Fragments arranged by size:
4319 3266 1609 210
SbfI CC_TGCA'GG
Cuts at: 6451 6451
Size: 9404
ScaI AGT'ACT
Cuts at: 8921 8921
Size: 9404
SexAI A'CCwGG_T
Cuts at: 5900 5900
Size: 9404
SfcI C'TryA_G
Cuts at: 33 851 1231 1450 1958 3245 4229 4612 4844
Size: 818 380 219 508 1287 984 383 232
Cuts at: 4844 5663 6447 7132 8125 8316 33
Size: 819 784 685 993 191 1121
Fragments arranged by size:
1287 1121 993 984 819 818 784 685
508 383 380 232 219 191
SfoI GGC'GCC
Cuts at: 6836 7084 6836
Size: 248 9156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9404
SmaI CCC'GGG
Cuts at: 550 6426 550
Size: 5876 3528
SmlI C'TyrA_G
Cuts at: 195 1537 1969 2046 2440 3745 4366 5104 5382
Size: 1342 432 77 394 1305 621 738 278
Cuts at: 5382 5461 6559 7474 7966 8228 195
Size: 79 1098 915 492 262 1371
Fragments arranged by size:
1371 1342 1305 1098 915 738 621 492
432 394 278 262 79 77
SnaBI TAC'GTA
Cuts at: 6396 6396
Size: 9404
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9404
SphI G_CATG'C
Cuts at: 6213 6457 6957 6213
Size: 244 500 8660
Fragments arranged by size:
8660 500 244
StuI AGG'CCT
Cuts at: 823 1050 1220 2287 2446 3010 3140 5380 5545
Size: 227 170 1067 159 564 130 2240 165
Cuts at: 5545 6358 823
Size: 813 3869
Fragments arranged by size:
3869 2240 1067 813 564 227 170 165
159 130
StyI C'CwwG_G
Cuts at: 125 246 747 1185 1316 2168 2362 2470 2684
Size: 121 501 438 131 852 194 108 214
Cuts at: 2684 5360 5576 6538 6892 125
Size: 2676 216 962 354 2637
Fragments arranged by size:
2676 2637 962 852 501 438 354 216
214 194 131 121 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 591 2116 2149 2746 3446 3788 4034 4676
Size: 440 1525 33 597 700 342 246 642
Cuts at: 4676 4772 6052 6206 6987 7120 7579 7788 151
Size: 96 1280 154 781 133 459 209 1767
Fragments arranged by size:
1767 1525 1280 781 700 642 597 459
440 342 246 209 154 133 96 33
TatI w'GTAC_w
Cuts at: 582 2838 8919 582
Size: 2256 6081 1067
Fragments arranged by size:
6081 2256 1067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 374 406 1274 1306 1631 1663 3098 3130 8921
Size: 32 868 32 325 32 1435 32 5791
Cuts at: 8921 8953 374
Size: 32 825
Fragments arranged by size:
5791 1435 868 825 325 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 7201 7201
Size: 9404
XbaI T'CTAG_A
Cuts at: 1824 6435 1824
Size: 4611 4793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1597 2946 5970 6899 1597
Size: 1349 3024 929 4102
Fragments arranged by size:
4102 3024 1349 929
XhoI C'TCGA_G
Cuts at: 4366 4366
Size: 9404
XmaI C'CCGG_G
Cuts at: 548 6424 548
Size: 5876 3528
XmnI GAAnn'nnTTC
Cuts at: 485 1180 485
Size: 695 8709
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AscI BmtI BsiWI BsrGI BssHII
ClaI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI PacI PmlI PshAI PsiI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI ZraI