Restriction map for
pF1KSDA0193
(Circular) (Six-base) MAPSORT of: pF1KSDA0193.seq Check: 4714 from: 1 to: 4379
pF1KSDA0193 4379 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1417 1417
Size: 4379
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2636 3383 3651 2204
Size: 432 747 268 2932
Fragments arranged by size:
2932 747 432 268
AflIII A'CryG_T
Cuts at: 1731 2835 3812 1731
Size: 1104 977 2298
Fragments arranged by size:
2298 1104 977
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 588 892 588
Size: 304 4075
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1005 1037 1117 1149 1342 1374 1005
Size: 32 80 32 193 32 4010
Fragments arranged by size:
4010 193 80 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1684 3251 1684
Size: 1567 2812
ApaLI G'TGCA_C
Cuts at: 285 3149 285
Size: 2864 1515
ApoI r'AATT_y
Cuts at: 204 1383 3732 3988 204
Size: 1179 2349 256 595
Fragments arranged by size:
2349 1179 595 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 628 1399 628
Size: 771 3608
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 1395 1809 2057 2092 1395
Size: 414 248 35 3682
Fragments arranged by size:
3682 414 248 35
BanII G_rGCy'C
Cuts at: 1393 2423 1393
Size: 1030 3349
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbvCI CC'TCA_GC
Cuts at: 1228 1228
Size: 4379
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 258 1899 258
Size: 1641 2738
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 289 1188 2004 2097 3153 289
Size: 899 816 93 1056 1515
Fragments arranged by size:
1515 1056 899 816 93
BmrI ACTGGGnnnn_n'
Cuts at: 657 1591 1672 2007 4151 657
Size: 934 81 335 2144 885
Fragments arranged by size:
2144 934 885 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 764 2746 764
Size: 1982 2397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1264 1270 791
Size: 473 6 3900
Fragments arranged by size:
3900 473 6
Bpu10I CC'TnA_GC
Cuts at: 176 1228 176
Size: 1052 3327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2926 3224 1555
Size: 879 492 298 2710
Fragments arranged by size:
2710 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 625 4083 625
Size: 3458 921
BsaAI yAC'GTr
Cuts at: 172 1189 1371 1732 172
Size: 1017 182 361 2819
Fragments arranged by size:
2819 1017 361 182
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 267 729 1568 2089 2767 3041 3188 3599 267
Size: 462 839 521 678 274 147 411 1047
Fragments arranged by size:
1047 839 678 521 462 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 950 980 1074 1104 1117 1147 1342 1372 950
Size: 30 94 30 13 30 195 30 3957
Fragments arranged by size:
3957 195 94 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 128 1245 128
Size: 1117 3262
BseYI C'CCAG_C
Cuts at: 447 1804 2562 3139 3635 447
Size: 1357 758 577 496 1191
Fragments arranged by size:
1357 1191 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 898
Size: 4379
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3175 104
Size: 1817 46 1208 1308
Fragments arranged by size:
1817 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 289 629 1237 1393 2171 2361 3153 289
Size: 340 608 156 778 190 792 1515
Fragments arranged by size:
1515 792 778 608 340 190 156
BsmI GAATG_Cn'
Cuts at: 995 995
Size: 4379
Bsp1286I G_dGCh'C
Cuts at: 289 629 1188 1237 1393 2004 2097 2171 2361
Size: 340 559 49 156 611 93 74 190
Cuts at: 2361 2423 3153 289
Size: 62 730 1515
Fragments arranged by size:
1515 730 611 559 340 190 156 93
74 62 49
BspEI T'CCGG_A
Cuts at: 729 3599 729
Size: 2870 1509
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1429 1643 1945 2326 1429
Size: 214 302 381 3482
Fragments arranged by size:
3482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 1154 2377 2558 2767 1154
Size: 1223 181 209 2766
Fragments arranged by size:
2766 1223 209 181
BsrGI T'GTAC_A
Cuts at: 1166 1166
Size: 4379
BssSI C'ACGA_G
Cuts at: 1177 2650 3008 1177
Size: 1473 358 2548
Fragments arranged by size:
2548 1473 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 110 2740 110
Size: 2630 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 714 714
Size: 4379
BstYI r'GATC_y
Cuts at: 1 456 1404 1871 1894 2229 2475 3487 3573
Size: 455 948 467 23 335 246 1012 86
Cuts at: 3573 3585 3931 1
Size: 12 346 449
Fragments arranged by size:
1012 948 467 455 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1271 125
Size: 1146 3233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 1622 1622
Size: 4379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 745 780 745
Size: 35 4344
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2943 2085
Size: 858 3521
EaeI y'GGCC_r
Cuts at: 1964 2138 2529 2556 3901 1964
Size: 174 391 27 1345 2442
Fragments arranged by size:
2442 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2897 3043 4189 2587
Size: 310 146 1146 2777
Fragments arranged by size:
2777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1264 1270 2204 2636 3383 3651 791
Size: 473 6 934 432 747 268 1519
Fragments arranged by size:
1519 934 747 473 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 317 1323 1518 317
Size: 1006 195 3178
Fragments arranged by size:
3178 1006 195
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 1813 2061 3083 4062 1813
Size: 248 1022 979 2130
Fragments arranged by size:
2130 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 591 623 950 982 2221 2251 2253
Size: 32 6 32 327 32 1239 30 2
Cuts at: 2253 2283 553
Size: 30 2649
Fragments arranged by size:
2649 1239 327 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 1344 1418 1344
Size: 74 4305
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 506 3050 3234 506
Size: 2544 184 1651
Fragments arranged by size:
2544 1651 184
MscI TGG'CCA
Cuts at: 2140 2140
Size: 4379
MslI CAynn'nnrTG
Cuts at: 712 874 2368 2495 712
Size: 162 1494 127 2596
Fragments arranged by size:
2596 1494 162 127
MspA1I CmG'CkG
Cuts at: 427 914 1490 1804 2164 3177 3422 427
Size: 487 576 314 360 1013 245 1384
Fragments arranged by size:
1384 1013 576 487 360 314 245
NaeI GCC'GGC
Cuts at: 1156 2560 1156
Size: 1404 2975
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 125 1271 125
Size: 1146 3233
NgoMIV G'CCGG_C
Cuts at: 1154 2558 1154
Size: 1404 2975
NspI r_CATG'y
Cuts at: 1432 1932 2839 1432
Size: 500 907 2972
Fragments arranged by size:
2972 907 500
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PfoI T'CCnGG_A
Cuts at: 1136 1136
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PmlI CAC'GTG
Cuts at: 172 1189 172
Size: 1017 3362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 766 798 1117 1149 1342 1374 3518 3550 766
Size: 32 319 32 193 32 2144 32 1595
Fragments arranged by size:
2144 1595 319 193 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 317 1323 317
Size: 1006 3373
PstI C_TGCA'G
Cuts at: 135 1426 2111 135
Size: 1291 685 2403
Fragments arranged by size:
2403 1291 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 427 1804 2164 427
Size: 1377 360 2642
Fragments arranged by size:
2642 1377 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 646 3896 646
Size: 3250 1129
SfcI C'TryA_G
Cuts at: 33 131 1422 2107 3100 3291 33
Size: 98 1291 685 993 191 1121
Fragments arranged by size:
1291 1121 993 685 191 98
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 628 1534 2449 2941 3203 628
Size: 906 915 492 262 1804
Fragments arranged by size:
1804 915 906 492 262
SnaBI TAC'GTA
Cuts at: 1371 1371
Size: 4379
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
StuI AGG'CCT
Cuts at: 1262 1262
Size: 4379
StyI C'CwwG_G
Cuts at: 125 313 756 1271 1513 1867 125
Size: 188 443 515 242 354 2637
Fragments arranged by size:
2637 515 443 354 242 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 612 1962 2095 2554 2763 612
Size: 1350 133 459 209 2228
Fragments arranged by size:
2228 1350 459 209 133
TatI w'GTAC_w
Cuts at: 644 1166 3894 644
Size: 522 2728 1129
Fragments arranged by size:
2728 1129 522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 822 1410 822
Size: 588 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1245 1874 1245
Size: 629 3750
XhoI C'TCGA_G
Cuts at: 628 628
Size: 4379
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XmnI ZraI