Restriction map for
pF1KSDA0194
(Circular) (Six-base) MAPSORT of: pF1KSDA0194.seq Check: 4693 from: 1 to: 7013
pF1KSDA0194 7013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2928 3124 3173 2928
Size: 196 49 6768
Fragments arranged by size:
6768 196 49
AccI GT'mk_AC
Cuts at: 2021 4051 2021
Size: 2030 4983
Acc65I G'GTAC_C
Cuts at: 4029 4029
Size: 7013
AclI AA'CG_TT
Cuts at: 6853 6853
Size: 7013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1813 1828 2668 2863 4838 5270 6017 6285 1813
Size: 15 840 195 1975 432 747 268 2541
Fragments arranged by size:
2541 1975 840 747 432 268 195 15
AflIII A'CryG_T
Cuts at: 4365 5469 6446 4365
Size: 1104 977 4932
Fragments arranged by size:
4932 1104 977
AgeI A'CCGG_T
Cuts at: 5401 5401
Size: 7013
AhdI GACnn_n'nnGTC
Cuts at: 1787 1787
Size: 7013
AleI CACnn'nnGTG
Cuts at: 1119 3439 1119
Size: 2320 4693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 684 1744 2230 684
Size: 1060 486 5467
Fragments arranged by size:
5467 1060 486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 577 609 1006 1038 577
Size: 32 397 32 6552
Fragments arranged by size:
6552 397 32 32
AlwNI CAG_nnn'CTG
Cuts at: 672 724 1326 2202 2470 2712 3073 3165 3592
Size: 52 602 876 268 242 361 92 427
Cuts at: 3592 4318 5885 672
Size: 726 1567 1800
Fragments arranged by size:
1800 1567 876 726 602 427 361 268
242 92 52
ApaI G_GGCC'C
Cuts at: 2426 2426
Size: 7013
ApaLI G'TGCA_C
Cuts at: 5783 5783
Size: 7013
ApoI r'AATT_y
Cuts at: 1263 1302 2045 2869 2971 4017 6366 6622 1263
Size: 39 743 824 102 1046 2349 256 1654
Fragments arranged by size:
2349 1654 1046 824 743 256 102 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 7013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7013
AvaI C'yCGr_G
Cuts at: 1417 4033 1417
Size: 2616 4397
AvrII C'CTAG_G
Cuts at: 1143 1143
Size: 7013
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 137 170 1421 1454 137
Size: 33 1251 33 5696
Fragments arranged by size:
5696 1251 33 33
BamHI G'GATC_C
Cuts at: 432 1157 4038 432
Size: 725 2881 3407
Fragments arranged by size:
3407 2881 725
BanI G'GyrC_C
Cuts at: 3112 3686 3717 4029 4443 4691 4726 3112
Size: 574 31 312 414 248 35 5399
Fragments arranged by size:
5399 574 414 312 248 35 31
BanII G_rGCy'C
Cuts at: 317 708 1617 2119 2153 2426 2881 3557 4027
Size: 391 909 502 34 273 455 676 470
Cuts at: 4027 5057 317
Size: 1030 2273
Fragments arranged by size:
2273 1030 909 676 502 470 455 391
273 34
BbeI G_GCGC'C
Cuts at: 4447 4695 4447
Size: 248 6765
BbsI GAAGACnn'nnnn_
Cuts at: 2244 2680 3206 2244
Size: 436 526 6051
Fragments arranged by size:
6051 526 436
BbvCI CC'TCA_GC
Cuts at: 560 692 754 2246 560
Size: 132 62 1492 5327
Fragments arranged by size:
5327 1492 132 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2023 2057 2023
Size: 34 6979
BciVI GTATCCnnnnn_n'
Cuts at: 4911 5672 7013 4911
Size: 761 1341 4911
Fragments arranged by size:
4911 1341 761
BclI T'GATC_A
Cuts at: 1379 4533 1379
Size: 3154 3859
BglI GCCn_nnn'nGGC
Cuts at: 653 1744 3994 653
Size: 1091 2250 3672
Fragments arranged by size:
3672 2250 1091
BglII A'GATC_T
Cuts at: 4528 6565 4528
Size: 2037 4976
BlpI GC'TnA_GC
Cuts at: 2695 4125 2695
Size: 1430 5583
Bme1580I G_kGCm'C
Cuts at: 644 890 1126 2426 3115 3319 3369 3689 3709
Size: 246 236 1300 689 204 50 320 20
Cuts at: 3709 4638 4731 5787 644
Size: 929 93 1056 1870
Fragments arranged by size:
1870 1300 1056 929 689 320 246 236
204 93 50 20
BmgBI CAC'GTC
Cuts at: 3283 3437 3283
Size: 154 6859
BmrI ACTGGGnnnn_n'
Cuts at: 708 1690 3607 3671 4225 4306 4641 6785 708
Size: 982 1917 64 554 81 335 2144 936
Fragments arranged by size:
2144 1917 982 936 554 335 81 64
BmtI G_CTAG'C
Cuts at: 2839 2839
Size: 7013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 575 969 2133 2349 3153 5380 575
Size: 394 1164 216 804 2227 2208
Fragments arranged by size:
2227 2208 1164 804 394 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1532 1536 1601 3088 1318
Size: 214 4 65 1487 5243
Fragments arranged by size:
5243 1487 214 65 4
Bpu10I CC'TnA_GC
Cuts at: 560 692 754 2155 2246 3169 560
Size: 132 62 1401 91 923 4404
Fragments arranged by size:
4404 1401 923 132 91 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5068 5560 5858 4189
Size: 879 492 298 5344
Fragments arranged by size:
5344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 832 2545 6717 832
Size: 1713 4172 1128
Fragments arranged by size:
4172 1713 1128
BsaAI yAC'GTr
Cuts at: 4005 4366 4005
Size: 361 6652
BsaBI GATnn'nnATC
Cuts at: 6 4527 6564 6
Size: 4521 2037 455
Fragments arranged by size:
4521 2037 455
BsaHI Gr'CG_yC
Cuts at: 1205 1447 4444 4692 5394 6890 1205
Size: 242 2997 248 702 1496 1328
Fragments arranged by size:
2997 1496 1328 702 248 242
BsaWI w'CCGG_w
Cuts at: 471 548 2081 4202 4723 5401 5675 5822 6233
Size: 77 1533 2121 521 678 274 147 411
Cuts at: 6233 471
Size: 1251
Fragments arranged by size:
2121 1533 1251 678 521 411 274 147
77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 848 878 1006 1036 1351 1381 1505 1535 2701
Size: 30 128 30 315 30 124 30 1166
Cuts at: 2701 2731 3011 3041 848
Size: 30 280 30 4820
Fragments arranged by size:
4820 1166 315 280 128 124 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 402 599 709 945 1328 1372 2323 2704 2832
Size: 197 110 236 383 44 951 381 128
Cuts at: 2832 2839 3032 3976 3979 3994 402
Size: 7 193 944 3 15 3421
Fragments arranged by size:
3421 951 944 383 381 236 197 193
128 110 44 15 7 3
BseYI C'CCAG_C
Cuts at: 643 1815 2053 2419 3682 4438 5196 5773 6269
Size: 1172 238 366 1263 756 758 577 496
Cuts at: 6269 643
Size: 1387
Fragments arranged by size:
1387 1263 1172 758 756 577 496 366
238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 437 944 1489 1929 2497 2677 3082 325
Size: 112 507 545 440 568 180 405 4256
Fragments arranged by size:
4256 568 545 507 440 405 180 112
BsiEI CG_ry'CG
Cuts at: 104 4555 4601 5809 104
Size: 4451 46 1208 1308
Fragments arranged by size:
4451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 317 770 1087 1617 1638 2119 2161 3557 3599
Size: 453 317 530 21 481 42 1396 42
Cuts at: 3599 4027 4805 4995 5787 317
Size: 428 778 190 792 1543
Fragments arranged by size:
1543 1396 792 778 530 481 453 428
317 190 42 42 21
BsmI GAATG_Cn'
Cuts at: 2145 2145
Size: 7013
BsmBI CGTCTCn'nnnn_
Cuts at: 1212 1212
Size: 7013
Bsp1286I G_dGCh'C
Cuts at: 317 644 708 770 890 1087 1126 1617 1638
Size: 327 64 62 120 197 39 491 21
Cuts at: 1638 2119 2153 2161 2426 2881 3115 3319 3369
Size: 481 34 8 265 455 234 204 50
Cuts at: 3369 3557 3599 3689 3709 4027 4638 4731 4805
Size: 188 42 90 20 318 611 93 74
Cuts at: 4805 4995 5057 5787 317
Size: 190 62 730 1543
Fragments arranged by size:
1543 730 611 491 481 455 327 318
265 234 204 197 190 188 120 93
90 74 64 62 62 50 42 39
34 21 20 8
BspEI T'CCGG_A
Cuts at: 471 6233 471
Size: 5762 1251
BspHI T'CATG_A
Cuts at: 929 2265 3011 6189 929
Size: 1336 746 3178 1753
Fragments arranged by size:
3178 1753 1336 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1601 2928 3124 3173 3392 4063 4277 4579 4960
Size: 1327 196 49 219 671 214 302 381
Cuts at: 4960 1601
Size: 3654
Fragments arranged by size:
3654 1327 671 381 302 219 214 196
49
BsrBI CCG'CTC
Cuts at: 50 5305 5359 6843 7008 50
Size: 5255 54 1484 165 55
Fragments arranged by size:
5255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2727 3782 4925 2727
Size: 1055 1143 4815
Fragments arranged by size:
4815 1143 1055
BsrFI r'CCGG_y
Cuts at: 5011 5192 5401 5011
Size: 181 209 6623
Fragments arranged by size:
6623 209 181
BsrGI T'GTAC_A
Cuts at: 726 3249 3879 726
Size: 2523 630 3860
Fragments arranged by size:
3860 2523 630
BssSI C'ACGA_G
Cuts at: 5284 5642 5284
Size: 358 6655
BstAPI GCAn_nnn'nTGC
Cuts at: 460 2202 2712 2950 3165 4390 460
Size: 1742 510 238 215 1225 3083
Fragments arranged by size:
3083 1742 1225 510 238 215
BstBI TT'CG_AA
Cuts at: 110 5374 110
Size: 5264 1749
BstEII G'GTnAC_C
Cuts at: 3670 3670
Size: 7013
BstXI CCAn_nnnn'nTGG
Cuts at: 3441 3441
Size: 7013
BstYI r'GATC_y
Cuts at: 1 432 494 749 1157 2174 3866 4038 4505
Size: 431 62 255 408 1017 1692 172 467
Cuts at: 4505 4528 4863 5109 6121 6207 6219 6565 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1692 1017 1012 467 449 431 408 346
335 255 246 172 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 1475 1475
Size: 7013
BtgI C'CryG_G
Cuts at: 125 1059 125
Size: 934 6079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4963 5144 5307 4963
Size: 181 163 6669
Fragments arranged by size:
6669 181 163
BtsI GCAGTG_nn'
Cuts at: 623 673 2191 2374 3084 3090 3181 3998 4256
Size: 50 1518 183 710 6 91 817 258
Cuts at: 4256 623
Size: 3380
Fragments arranged by size:
3380 1518 817 710 258 183 91 50
6
DraI TTT'AAA
Cuts at: 4012 4012
Size: 7013
DraIII CAC_nnn'GTG
Cuts at: 2369 2369
Size: 7013
DrdI GACnn_nn'nnGTC
Cuts at: 4719 5577 4719
Size: 858 6155
EaeI y'GGCC_r
Cuts at: 380 3406 3431 3986 4598 4772 5163 5190 6535
Size: 3026 25 555 612 174 391 27 1345
Cuts at: 6535 380
Size: 858
Fragments arranged by size:
3026 1345 858 612 555 391 174 27
25
EagI C'GGCC_G
Cuts at: 4598 4598
Size: 7013
EarI CTCTTCn'nnn_
Cuts at: 830 1215 2839 3143 3399 3971 5036 5246 830
Size: 385 1624 304 256 572 1065 210 2597
Fragments arranged by size:
2597 1624 1065 572 385 304 256 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3760 5221 5531 5677 6823 3760
Size: 1461 310 146 1146 3950
Fragments arranged by size:
3950 1461 1146 310 146
EcoICRI GAG'CTC
Cuts at: 315 1615 2117 3555 4025 315
Size: 1300 502 1438 470 3303
Fragments arranged by size:
3303 1438 1300 502 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1532 1536 1601 1813 1828 2668 2863 3088
Size: 214 4 65 212 15 840 195 225
Cuts at: 3088 4838 5270 6017 6285 1318
Size: 1750 432 747 268 2046
Fragments arranged by size:
2046 1750 840 747 432 268 225 214
212 195 65 15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 541 691 757 1776 3093 541
Size: 150 66 1019 1317 4461
Fragments arranged by size:
4461 1317 1019 150 66
EcoO109I rG'GnC_Cy
Cuts at: 604 684 2423 2619 4152 604
Size: 80 1739 196 1533 3465
Fragments arranged by size:
3465 1739 1533 196 80
EcoRI G'AATT_C
Cuts at: 1302 2869 4017 1302
Size: 1567 1148 4298
Fragments arranged by size:
4298 1567 1148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5032 5032
Size: 7013
FspI TGC'GCA
Cuts at: 2206 4794 2206
Size: 2588 4425
HaeII r_GCGC'y
Cuts at: 2184 4447 4695 5717 6696 2184
Size: 2263 248 1022 979 2501
Fragments arranged by size:
2501 2263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 898 930 1773 1805 2218 2250 2344
Size: 32 442 32 843 32 413 32 94
Cuts at: 2344 2376 2618 2650 3011 3043 4855 4885 4887
Size: 32 242 32 361 32 1812 30 2
Cuts at: 4887 4917 424
Size: 30 2520
Fragments arranged by size:
2520 1812 843 442 413 361 242 94
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1590 1701 2067 3033 4052 1590
Size: 111 366 966 1019 4551
Fragments arranged by size:
4551 1019 966 366 111
HindIII A'AGCT_T
Cuts at: 3121 3121
Size: 7013
HpaI GTT'AAC
Cuts at: 1701 2067 1701
Size: 366 6647
KasI G'GCGC_C
Cuts at: 4443 4691 4443
Size: 248 6765
KpnI G_GTAC'C
Cuts at: 4033 4033
Size: 7013
MluI A'CGCG_T
Cuts at: 6446 6446
Size: 7013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 343 644 1901 2321 5684 5868 343
Size: 301 1257 420 3363 184 1488
Fragments arranged by size:
3363 1488 1257 420 301 184
MscI TGG'CCA
Cuts at: 3433 4774 3433
Size: 1341 5672
MslI CAynn'nnrTG
Cuts at: 1119 3439 5002 5129 1119
Size: 2320 1563 127 3003
Fragments arranged by size:
3003 2320 1563 127
MspA1I CmG'CkG
Cuts at: 1815 1943 2057 2199 2703 3777 4124 4438 4798
Size: 128 114 142 504 1074 347 314 360
Cuts at: 4798 5811 6056 1815
Size: 1013 245 2772
Fragments arranged by size:
2772 1074 1013 504 360 347 314 245
142 128 114
NaeI GCC'GGC
Cuts at: 5194 5194
Size: 7013
NarI GG'CG_CC
Cuts at: 4444 4692 4444
Size: 248 6765
NcoI C'CATG_G
Cuts at: 125 1059 125
Size: 934 6079
NdeI CA'TA_TG
Cuts at: 138 1095 138
Size: 957 6056
NgoMIV G'CCGG_C
Cuts at: 5192 5192
Size: 7013
NheI G'CTAG_C
Cuts at: 2835 2835
Size: 7013
NspI r_CATG'y
Cuts at: 1070 4066 4566 5473 1070
Size: 2996 500 907 2610
Fragments arranged by size:
2996 2610 907 500
PasI CC'CwG_GG
Cuts at: 1078 1603 1733 1078
Size: 525 130 6358
Fragments arranged by size:
6358 525 130
PciI A'CATG_T
Cuts at: 5469 5469
Size: 7013
PflMI CCAn_nnn'nTGG
Cuts at: 1401 2505 3073 3327 3696 1401
Size: 1104 568 254 369 4718
Fragments arranged by size:
4718 1104 568 369 254
PfoI T'CCnGG_A
Cuts at: 1509 3104 1509
Size: 1595 5418
PmeI GTTT'AAAC
Cuts at: 4012 4012
Size: 7013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1006 1038 6152 6184 1006
Size: 32 5114 32 1835
Fragments arranged by size:
5114 1835 32 32
PpuMI rG'GwC_Cy
Cuts at: 604 2619 604
Size: 2015 4998
PshAI GACnn'nnGTC
Cuts at: 1450 1450
Size: 7013
PspOMI G'GGCC_C
Cuts at: 2422 2422
Size: 7013
PstI C_TGCA'G
Cuts at: 542 1665 2198 2219 2462 2708 3161 4060 4745
Size: 1123 533 21 243 246 453 899 685
Cuts at: 4745 542
Size: 2810
Fragments arranged by size:
2810 1123 899 685 533 453 246 243
21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7013
PvuII CAG'CTG
Cuts at: 1815 1943 2057 2199 2703 4438 4798 1815
Size: 128 114 142 504 1735 360 4030
Fragments arranged by size:
4030 1735 504 360 142 128 114
RsrII CG'GwC_CG
Cuts at: 5208 5208
Size: 7013
SacI G_AGCT'C
Cuts at: 317 1617 2119 3557 4027 317
Size: 1300 502 1438 470 3303
Fragments arranged by size:
3303 1438 1300 502 470
SalI G'TCGA_C
Cuts at: 4050 4050
Size: 7013
SapI GCTCTTCn'nnn_
Cuts at: 2839 5036 5246 2839
Size: 2197 210 4606
Fragments arranged by size:
4606 2197 210
SbfI CC_TGCA'GG
Cuts at: 542 4060 542
Size: 3518 3495
ScaI AGT'ACT
Cuts at: 6530 6530
Size: 7013
SfcI C'TryA_G
Cuts at: 33 538 1580 1661 2194 2215 2379 2458 2704
Size: 505 1042 81 533 21 164 79 246
Cuts at: 2704 3141 3157 3655 4056 4741 5734 5925 33
Size: 437 16 498 401 685 993 191 1121
Fragments arranged by size:
1121 1042 993 685 533 505 498 437
401 246 191 164 81 79 21 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 1744 1744
Size: 7013
SfoI GGC'GCC
Cuts at: 4445 4693 4445
Size: 248 6765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7013
SmaI CCC'GGG
Cuts at: 1419 4035 1419
Size: 2616 4397
SmlI C'TyrA_G
Cuts at: 4168 5083 5575 5837 4168
Size: 915 492 262 5344
Fragments arranged by size:
5344 915 492 262
SnaBI TAC'GTA
Cuts at: 4005 4005
Size: 7013
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7013
SphI G_CATG'C
Cuts at: 4066 4566 4066
Size: 500 6513
StuI AGG'CCT
Cuts at: 1747 3653 1747
Size: 1906 5107
StyI C'CwwG_G
Cuts at: 125 842 1059 1143 2099 3089 3206 3695 3930
Size: 717 217 84 956 990 117 489 235
Cuts at: 3930 4147 4501 125
Size: 217 354 2637
Fragments arranged by size:
2637 990 956 717 489 354 235 217
217 117 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 225 3626 4596 4729 5188 5397 225
Size: 3401 970 133 459 209 1841
Fragments arranged by size:
3401 1841 970 459 209 133
TatI w'GTAC_w
Cuts at: 278 726 1937 2133 3249 3879 6528 278
Size: 448 1211 196 1116 630 2649 763
Fragments arranged by size:
2649 1211 1116 763 630 448 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 848 880 1351 1383 2701 2733 6530 6562 848
Size: 32 471 32 1318 32 3797 32 1299
Fragments arranged by size:
3797 1318 1299 471 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4810 4810
Size: 7013
XbaI T'CTAG_A
Cuts at: 4044 4044
Size: 7013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 898 1185 1221 1293 2805 4508 876
Size: 22 287 36 72 1512 1703 3381
Fragments arranged by size:
3381 1703 1512 287 72 36 22
XmaI C'CCGG_G
Cuts at: 1417 4033 1417
Size: 2616 4397
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AscI BfrBI BsiWI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI MfeI NotI NruI
NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SrfI SspI SwaI XhoI XmnI ZraI