Restriction map for
pF1KSDA0195
(Circular) (Six-base) MAPSORT of: pF1KSDA0195.seq Check: 8347 from: 1 to: 7205
pF1KSDA0195 7205 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3017 3017
Size: 7205
AccI GT'mk_AC
Cuts at: 2681 4243 2681
Size: 1562 5643
Acc65I G'GTAC_C
Cuts at: 4221 4221
Size: 7205
AclI AA'CG_TT
Cuts at: 7045 7045
Size: 7205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 223 502 1014 1413 1665 5030 5462 6209
Size: 69 279 512 399 252 3365 432 747
Cuts at: 6209 6477 154
Size: 268 882
Fragments arranged by size:
3365 882 747 512 432 399 279 268
252 69
AflIII A'CryG_T
Cuts at: 4557 5661 6638 4557
Size: 1104 977 5124
Fragments arranged by size:
5124 1104 977
AgeI A'CCGG_T
Cuts at: 5593 5593
Size: 7205
AhdI GACnn_n'nnGTC
Cuts at: 646 646
Size: 7205
AleI CACnn'nnGTG
Cuts at: 3877 3877
Size: 7205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3556 3556
Size: 7205
AlwNI CAG_nnn'CTG
Cuts at: 925 1417 2226 2519 2577 3671 3946 4155 4510
Size: 492 809 293 58 1094 275 209 355
Cuts at: 4510 6077 925
Size: 1567 2053
Fragments arranged by size:
2053 1567 1094 809 492 355 293 275
209 58
ApaLI G'TGCA_C
Cuts at: 5975 5975
Size: 7205
ApoI r'AATT_y
Cuts at: 4209 6558 6814 4209
Size: 2349 256 4600
Fragments arranged by size:
4600 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7205
AvaI C'yCGr_G
Cuts at: 803 1038 1294 1507 1615 2301 2858 2981 3719
Size: 235 256 213 108 686 557 123 738
Cuts at: 3719 4225 803
Size: 506 3783
Fragments arranged by size:
3783 738 686 557 506 256 235 213
123 108
BamHI G'GATC_C
Cuts at: 1385 3189 4230 1385
Size: 1804 1041 4360
Fragments arranged by size:
4360 1804 1041
BanI G'GyrC_C
Cuts at: 893 2236 2439 4221 4635 4883 4918 893
Size: 1343 203 1782 414 248 35 3180
Fragments arranged by size:
3180 1782 1343 414 248 203 35
BanII G_rGCy'C
Cuts at: 835 1351 1448 1514 1622 1715 2527 2705 2879
Size: 516 97 66 108 93 812 178 174
Cuts at: 2879 3780 3913 4219 5249 835
Size: 901 133 306 1030 2791
Fragments arranged by size:
2791 1030 901 812 516 306 178 174
133 108 97 93 66
BbeI G_GCGC'C
Cuts at: 4639 4887 4639
Size: 248 6957
BbsI GAAGACnn'nnnn_
Cuts at: 2567 3619 3852 2567
Size: 1052 233 5920
Fragments arranged by size:
5920 1052 233
BbvCI CC'TCA_GC
Cuts at: 162 193 2170 162
Size: 31 1977 5197
Fragments arranged by size:
5197 1977 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2406 2440 2406
Size: 34 7171
BciVI GTATCCnnnnn_n'
Cuts at: 590 1209 2998 5103 5864 7205 590
Size: 619 1789 2105 761 1341 590
Fragments arranged by size:
2105 1789 1341 761 619 590
BclI T'GATC_A
Cuts at: 2180 4725 2180
Size: 2545 4660
BglI GCCn_nnn'nGGC
Cuts at: 1534 2036 2825 1534
Size: 502 789 5914
Fragments arranged by size:
5914 789 502
BglII A'GATC_T
Cuts at: 2588 4720 6757 2588
Size: 2132 2037 3036
Fragments arranged by size:
3036 2132 2037
BlpI GC'TnA_GC
Cuts at: 1315 1792 3529 4317 1315
Size: 477 1737 788 4203
Fragments arranged by size:
4203 1737 788 477
Bme1580I G_kGCm'C
Cuts at: 983 1038 2006 2444 3032 3044 4043 4830 4923
Size: 55 968 438 588 12 999 787 93
Cuts at: 4923 5979 983
Size: 1056 2209
Fragments arranged by size:
2209 1056 999 968 787 588 438 93
55 12
BmgBI CAC'GTC
Cuts at: 263 4017 4038 263
Size: 3754 21 3430
Fragments arranged by size:
3754 3430 21
BmrI ACTGGGnnnn_n'
Cuts at: 563 633 2096 2607 4417 4498 4833 6977 563
Size: 70 1463 511 1810 81 335 2144 791
Fragments arranged by size:
2144 1810 1463 791 511 335 81 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 177 1260 1546 2919 5572 177
Size: 1083 286 1373 2653 1810
Fragments arranged by size:
2653 1810 1373 1083 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 778 1054 1060 1804 2541 2572 2758 2793
Size: 543 276 6 744 737 31 186 35
Cuts at: 2793 2974 3148 3580 3764 4051 235
Size: 181 174 432 184 287 3389
Fragments arranged by size:
3389 744 737 543 432 287 276 186
184 181 174 35 31 6
Bpu10I CC'TnA_GC
Cuts at: 162 193 2170 2578 162
Size: 31 1977 408 4789
Fragments arranged by size:
4789 1977 408 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 3631 4381 5260 5752 6050 883
Size: 2748 750 879 492 298 2038
Fragments arranged by size:
2748 2038 879 750 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 761 859 3061 3214 3331 6909 761
Size: 98 2202 153 117 3578 1057
Fragments arranged by size:
3578 2202 1057 153 117 98
BsaAI yAC'GTr
Cuts at: 4197 4558 4197
Size: 361 6844
BsaBI GATnn'nnATC
Cuts at: 6 3088 4719 6756 6
Size: 3082 1631 2037 455
Fragments arranged by size:
3082 2037 1631 455
BsaHI Gr'CG_yC
Cuts at: 1088 1526 4636 4884 5586 7082 1088
Size: 438 3110 248 702 1496 1211
Fragments arranged by size:
3110 1496 1211 702 438 248
BsaWI w'CCGG_w
Cuts at: 4394 4915 5593 5867 6014 6425 4394
Size: 521 678 274 147 411 5174
Fragments arranged by size:
5174 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 547 577 1849 1879 2812 2842 3730
Size: 30 222 30 1272 30 933 30 888
Cuts at: 3730 3760 295
Size: 30 3740
Fragments arranged by size:
3740 1272 933 888 222 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1057 1236 2869 2872 2905 2908 3349 3735 1057
Size: 179 1633 3 33 3 441 386 4527
Fragments arranged by size:
4527 1633 441 386 179 33 3 3
BseYI C'CCAG_C
Cuts at: 366 836 1186 1405 1714 1724 2130 2479 2815
Size: 470 350 219 309 10 406 349 336
Cuts at: 2815 2944 3543 3617 3760 3957 4173 4630 5388
Size: 129 599 74 143 197 216 457 758
Cuts at: 5388 5965 6461 366
Size: 577 496 1110
Fragments arranged by size:
1110 758 599 577 496 470 457 406
350 349 336 309 219 216 197 143
129 74 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1321 1543 1840 2247 3042 3835 973
Size: 348 222 297 407 795 793 4343
Fragments arranged by size:
4343 795 793 407 348 297 222
BsiEI CG_ry'CG
Cuts at: 104 3836 4747 4793 6001 104
Size: 3732 911 46 1208 1308
Fragments arranged by size:
3732 1308 1208 911 46
BsiHKAI G_wGCw'C
Cuts at: 461 749 1295 2527 2705 4219 4997 5187 5979
Size: 288 546 1232 178 1514 778 190 792
Cuts at: 5979 461
Size: 1687
Fragments arranged by size:
1687 1514 1232 792 778 546 288 190
178
BsmI GAATG_Cn'
Cuts at: 423 423
Size: 7205
BsmBI CGTCTCn'nnnn_
Cuts at: 629 1517 629
Size: 888 6317
Bsp1286I G_dGCh'C
Cuts at: 461 749 835 983 1038 1295 1351 1448 1514
Size: 288 86 148 55 257 56 97 66
Cuts at: 1514 1622 1715 2006 2444 2527 2705 2879 3032
Size: 108 93 291 438 83 178 174 153
Cuts at: 3032 3044 3780 3913 4043 4219 4830 4923 4997
Size: 12 736 133 130 176 611 93 74
Cuts at: 4997 5187 5249 5979 461
Size: 190 62 730 1687
Fragments arranged by size:
1687 736 730 611 438 291 288 257
190 178 176 174 153 148 133 130
108 97 93 93 86 83 74 66
62 56 55 12
BspEI T'CCGG_A
Cuts at: 6425 6425
Size: 7205
BspHI T'CATG_A
Cuts at: 6381 6381
Size: 7205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 662 3017 3124 3149 3995 4255 4469 4771 5152
Size: 2355 107 25 846 260 214 302 381
Cuts at: 5152 662
Size: 2715
Fragments arranged by size:
2715 2355 846 381 302 260 214 107
25
BsrBI CCG'CTC
Cuts at: 50 246 501 942 1493 2582 5497 5551 7035
Size: 196 255 441 551 1089 2915 54 1484
Cuts at: 7035 7200 50
Size: 165 55
Fragments arranged by size:
2915 1484 1089 551 441 255 196 165
55 54
BsrDI GCAATG_nn'
Cuts at: 1958 3179 5117 1958
Size: 1221 1938 4046
Fragments arranged by size:
4046 1938 1221
BsrFI r'CCGG_y
Cuts at: 476 845 994 5203 5384 5593 476
Size: 369 149 4209 181 209 2088
Fragments arranged by size:
4209 2088 369 209 181 149
BssSI C'ACGA_G
Cuts at: 5476 5834 5476
Size: 358 6847
BstAPI GCAn_nnn'nTGC
Cuts at: 4582 4582
Size: 7205
BstBI TT'CG_AA
Cuts at: 110 5566 110
Size: 5456 1749
BstEII G'GTnAC_C
Cuts at: 3111 4089 3111
Size: 978 6227
BstXI CCAn_nnnn'nTGG
Cuts at: 1084 1124 2952 1084
Size: 40 1828 5337
Fragments arranged by size:
5337 1828 40
BstYI r'GATC_y
Cuts at: 1 557 1385 2305 2588 2798 3189 4230 4697
Size: 556 828 920 283 210 391 1041 467
Cuts at: 4697 4720 5055 5301 6313 6399 6411 6757 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1041 1012 920 828 556 467 449 391
346 335 283 246 210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 541 1285 541
Size: 744 6461
BtgI C'CryG_G
Cuts at: 125 399 983 2232 125
Size: 274 584 1249 5098
Fragments arranged by size:
5098 1249 584 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1289 2111 3868 5155 5336 5499 1289
Size: 822 1757 1287 181 163 2995
Fragments arranged by size:
2995 1757 1287 822 181 163
BtsI GCAGTG_nn'
Cuts at: 227 923 3424 3694 4448 227
Size: 696 2501 270 754 2984
Fragments arranged by size:
2984 2501 754 696 270
DraI TTT'AAA
Cuts at: 4204 4204
Size: 7205
DraIII CAC_nnn'GTG
Cuts at: 2468 2997 2468
Size: 529 6676
DrdI GACnn_nn'nnGTC
Cuts at: 4015 4911 5769 4015
Size: 896 858 5451
Fragments arranged by size:
5451 896 858
EaeI y'GGCC_r
Cuts at: 325 350 702 992 1396 1644 2445 2583 3833
Size: 25 352 290 404 248 801 138 1250
Cuts at: 3833 4790 4964 5355 5382 6727 325
Size: 957 174 391 27 1345 803
Fragments arranged by size:
1345 1250 957 803 801 404 391 352
290 248 174 138 27 25
EagI C'GGCC_G
Cuts at: 3833 4790 3833
Size: 957 6248
EarI CTCTTCn'nnn_
Cuts at: 778 1703 2194 3163 3412 5228 5438 778
Size: 925 491 969 249 1816 210 2545
Fragments arranged by size:
2545 1816 969 925 491 249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5413 5723 5869 7015 5413
Size: 310 146 1146 5603
Fragments arranged by size:
5603 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2525 2703 4217 2525
Size: 178 1514 5513
Fragments arranged by size:
5513 1514 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 223 235 502 778 1014 1054 1060 1413
Size: 69 12 267 276 236 40 6 353
Cuts at: 1413 1665 1804 2541 2572 2758 2793 2974 3148
Size: 252 139 737 31 186 35 181 174
Cuts at: 3148 3580 3764 4051 5030 5462 6209 6477 154
Size: 432 184 287 979 432 747 268 882
Fragments arranged by size:
979 882 747 737 432 432 353 287
276 268 267 252 236 186 184 181
174 139 69 40 35 31 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1801 2848 2887 1801
Size: 1047 39 6119
Fragments arranged by size:
6119 1047 39
EcoO109I rG'GnC_Cy
Cuts at: 189 332 1467 2010 2574 4344 189
Size: 143 1135 543 564 1770 3050
Fragments arranged by size:
3050 1770 1135 564 543 143
EcoRI G'AATT_C
Cuts at: 4209 4209
Size: 7205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3930 5224 3930
Size: 1294 5911
FspI TGC'GCA
Cuts at: 4986 4986
Size: 7205
HaeII r_GCGC'y
Cuts at: 1929 1985 2089 4639 4887 5909 6888 1929
Size: 56 104 2550 248 1022 979 2246
Fragments arranged by size:
2550 2246 1022 979 248 104 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 632 664 1468 1500 2273 2305 5047 5077 5079
Size: 32 804 32 773 32 2742 30 2
Cuts at: 5079 5109 632
Size: 30 2728
Fragments arranged by size:
2742 2728 804 773 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 651 2661 4244 651
Size: 2010 1583 3612
Fragments arranged by size:
3612 2010 1583
KasI G'GCGC_C
Cuts at: 4635 4883 4635
Size: 248 6957
KpnI G_GTAC'C
Cuts at: 4225 4225
Size: 7205
MluI A'CGCG_T
Cuts at: 6638 6638
Size: 7205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1694 1868 1967 2987 5876 6060 1694
Size: 174 99 1020 2889 184 2839
Fragments arranged by size:
2889 2839 1020 184 174 99
MscI TGG'CCA
Cuts at: 704 1398 4966 704
Size: 694 3568 2943
Fragments arranged by size:
3568 2943 694
MslI CAynn'nnrTG
Cuts at: 2599 3247 3574 3877 5194 5321 2599
Size: 648 327 303 1317 127 4483
Fragments arranged by size:
4483 1317 648 327 303 127
MspA1I CmG'CkG
Cuts at: 213 308 1433 1854 2242 2519 3152 3285 3426
Size: 95 1125 421 388 277 633 133 141
Cuts at: 3426 3468 3993 4316 4630 4990 6003 6248 213
Size: 42 525 323 314 360 1013 245 1170
Fragments arranged by size:
1170 1125 1013 633 525 421 388 360
323 314 277 245 141 133 95 42
NaeI GCC'GGC
Cuts at: 996 5386 996
Size: 4390 2815
NarI GG'CG_CC
Cuts at: 4636 4884 4636
Size: 248 6957
NcoI C'CATG_G
Cuts at: 125 2232 125
Size: 2107 5098
NgoMIV G'CCGG_C
Cuts at: 994 5384 994
Size: 4390 2815
NspI r_CATG'y
Cuts at: 597 2571 2787 3379 4258 4758 5665 597
Size: 1974 216 592 879 500 907 2137
Fragments arranged by size:
2137 1974 907 879 592 500 216
PasI CC'CwG_GG
Cuts at: 168 1878 2006 168
Size: 1710 128 5367
Fragments arranged by size:
5367 1710 128
PciI A'CATG_T
Cuts at: 5661 5661
Size: 7205
PflMI CCAn_nnn'nTGG
Cuts at: 3926 3926
Size: 7205
PfoI T'CCnGG_A
Cuts at: 755 2951 755
Size: 2196 5009
PmeI GTTT'AAAC
Cuts at: 4204 4204
Size: 7205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1308 1340 6344 6376 1308
Size: 32 5004 32 2137
Fragments arranged by size:
5004 2137 32 32
PpuMI rG'GwC_Cy
Cuts at: 1467 1467
Size: 7205
PshAI GACnn'nnGTC
Cuts at: 3813 3813
Size: 7205
PstI C_TGCA'G
Cuts at: 3014 3284 3419 3701 3992 4160 4252 4937 3014
Size: 270 135 282 291 168 92 685 5282
Fragments arranged by size:
5282 685 291 282 270 168 135 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7205
PvuII CAG'CTG
Cuts at: 213 1854 2519 3152 3285 3426 3468 3993 4630
Size: 1641 665 633 133 141 42 525 637
Cuts at: 4630 4990 213
Size: 360 2428
Fragments arranged by size:
2428 1641 665 637 633 525 360 141
133 42
RsrII CG'GwC_CG
Cuts at: 5400 5400
Size: 7205
SacI G_AGCT'C
Cuts at: 2527 2705 4219 2527
Size: 178 1514 5513
Fragments arranged by size:
5513 1514 178
SalI G'TCGA_C
Cuts at: 4242 4242
Size: 7205
SapI GCTCTTCn'nnn_
Cuts at: 1703 5228 5438 1703
Size: 3525 210 3470
Fragments arranged by size:
3525 3470 210
SbfI CC_TGCA'GG
Cuts at: 4252 4252
Size: 7205
ScaI AGT'ACT
Cuts at: 6722 6722
Size: 7205
SexAI A'CCwGG_T
Cuts at: 644 644
Size: 7205
SfcI C'TryA_G
Cuts at: 33 630 3010 3280 3415 3697 3988 4156 4248
Size: 597 2380 270 135 282 291 168 92
Cuts at: 4248 4933 5926 6117 33
Size: 685 993 191 1121
Fragments arranged by size:
2380 1121 993 685 597 291 282 270
191 168 135 92
SfoI GGC'GCC
Cuts at: 4637 4885 4637
Size: 248 6957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7205
SmaI CCC'GGG
Cuts at: 805 1040 2983 3721 4227 805
Size: 235 1943 738 506 3783
Fragments arranged by size:
3783 1943 738 506 235
SmlI C'TyrA_G
Cuts at: 862 3646 4360 5275 5767 6029 862
Size: 2784 714 915 492 262 2038
Fragments arranged by size:
2784 2038 915 714 492 262
SnaBI TAC'GTA
Cuts at: 4197 4197
Size: 7205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7205
SphI G_CATG'C
Cuts at: 2571 3379 4258 4758 2571
Size: 808 879 500 5018
Fragments arranged by size:
5018 879 808 500
StuI AGG'CCT
Cuts at: 699 1180 2260 3259 699
Size: 481 1080 999 4645
Fragments arranged by size:
4645 1080 999 481
StyI C'CwwG_G
Cuts at: 125 2054 2232 4339 4693 125
Size: 1929 178 2107 354 2637
Fragments arranged by size:
2637 2107 1929 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1200 1673 2787 3889 4788 4921 5380 5589 1200
Size: 473 1114 1102 899 133 459 209 2816
Fragments arranged by size:
2816 1114 1102 899 473 459 209 133
TatI w'GTAC_w
Cuts at: 1058 1419 6720 1058
Size: 361 5301 1543
Fragments arranged by size:
5301 1543 361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 547 579 1753 1785 1849 1881 2812 2844 6722
Size: 32 1174 32 64 32 931 32 3878
Cuts at: 6722 6754 547
Size: 32 998
Fragments arranged by size:
3878 1174 998 931 64 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5002 5002
Size: 7205
XbaI T'CTAG_A
Cuts at: 4236 4236
Size: 7205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1194 1639 3246 3262 3573 4021 4700 1194
Size: 445 1607 16 311 448 679 3699
Fragments arranged by size:
3699 1607 679 448 445 311 16
XmaI C'CCGG_G
Cuts at: 803 1038 2981 3719 4225 803
Size: 235 1943 738 506 3783
Fragments arranged by size:
3783 1943 738 506 235
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BfrBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI