Restriction map for
pF1KSDA0198
(Circular) (Six-base) MAPSORT of: pF1KSDA0198.seq Check: 1112 from: 1 to: 4625
pF1KSDA0198 4625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 2450 2882 3629 3897 670
Size: 1780 432 747 268 1398
Fragments arranged by size:
1780 1398 747 432 268
AflIII A'CryG_T
Cuts at: 829 890 1977 3081 4058 829
Size: 61 1087 1104 977 1396
Fragments arranged by size:
1396 1104 1087 977 61
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AleI CACnn'nnGTG
Cuts at: 701 1051 701
Size: 350 4275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1069 1603 1069
Size: 534 4091
AlwNI CAG_nnn'CTG
Cuts at: 636 861 1470 1513 1930 3497 636
Size: 225 609 43 417 1567 1764
Fragments arranged by size:
1764 1567 609 417 225 43
ApaLI G'TGCA_C
Cuts at: 760 1069 3395 760
Size: 309 2326 1990
Fragments arranged by size:
2326 1990 309
ApoI r'AATT_y
Cuts at: 254 1629 3978 4234 254
Size: 1375 2349 256 645
Fragments arranged by size:
2349 1375 645 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 212 965 1645 212
Size: 753 680 3192
Fragments arranged by size:
3192 753 680
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 554 679 749 1021 1641 2055 2303 2338 554
Size: 125 70 272 620 414 248 35 2841
Fragments arranged by size:
2841 620 414 272 248 125 70 35
BanII G_rGCy'C
Cuts at: 884 946 1324 1639 2669 884
Size: 62 378 315 1030 2840
Fragments arranged by size:
2840 1030 378 315 62
BbeI G_GCGC'C
Cuts at: 558 2059 2307 558
Size: 1501 248 2876
Fragments arranged by size:
2876 1501 248
BbsI GAAGACnn'nnnn_
Cuts at: 1551 1551
Size: 4625
BbvCI CC'TCA_GC
Cuts at: 289 338 592 1271 1457 289
Size: 49 254 679 186 3457
Fragments arranged by size:
3457 679 254 186 49
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3284 4625 2523
Size: 761 1341 2523
Fragments arranged by size:
2523 1341 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BfrBI ATG'CAT
Cuts at: 570 570
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 940 1133 1490 1737 940
Size: 193 357 247 3828
Fragments arranged by size:
3828 357 247 193
Bme1580I G_kGCm'C
Cuts at: 764 801 1026 1073 2250 2343 3399 764
Size: 37 225 47 1177 93 1056 1990
Fragments arranged by size:
1990 1177 1056 225 93 47 37
BmgBI CAC'GTC
Cuts at: 1565 1565
Size: 4625
BmrI ACTGGGnnnn_n'
Cuts at: 1387 1837 1918 2253 4397 1387
Size: 450 81 335 2144 1615
Fragments arranged by size:
2144 1615 450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1118 2992 1118
Size: 1874 2751
Bpu10I CC'TnA_GC
Cuts at: 289 338 592 1271 1457 289
Size: 49 254 679 186 3457
Fragments arranged by size:
3457 679 254 186 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1481 1801 2680 3172 3470 850
Size: 631 320 879 492 298 2005
Fragments arranged by size:
2005 879 631 492 320 298
BsaI GGTCTCn'nnnn_
Cuts at: 315 4329 315
Size: 4014 611
BsaAI yAC'GTr
Cuts at: 1617 1978 1617
Size: 361 4264
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 555 2056 2304 3006 4502 555
Size: 1501 248 702 1496 678
Fragments arranged by size:
1501 1496 702 678 248
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3287 3434 3845 1814
Size: 521 678 274 147 411 2594
Fragments arranged by size:
2594 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1421 1451 1421
Size: 30 4595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 193
Size: 4625
BseYI C'CCAG_C
Cuts at: 543 800 1042 1128 1256 1513 2050 2808 3385
Size: 257 242 86 128 257 537 758 577
Cuts at: 3385 3881 543
Size: 496 1287
Fragments arranged by size:
1287 758 577 537 496 257 257 242
128 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1525 328
Size: 1197 3428
BsiEI CG_ry'CG
Cuts at: 104 717 2167 2213 3421 104
Size: 613 1450 46 1208 1308
Fragments arranged by size:
1450 1308 1208 613 46
BsiHKAI G_wGCw'C
Cuts at: 647 764 1073 1639 2417 2607 3399 647
Size: 117 309 566 778 190 792 1873
Fragments arranged by size:
1873 792 778 566 309 190 117
BsmI GAATG_Cn'
Cuts at: 1606 1606
Size: 4625
Bsp1286I G_dGCh'C
Cuts at: 647 764 801 884 946 1026 1073 1324 1639
Size: 117 37 83 62 80 47 251 315
Cuts at: 1639 2250 2343 2417 2607 2669 3399 647
Size: 611 93 74 190 62 730 1873
Fragments arranged by size:
1873 730 611 315 251 190 117 93
83 80 74 62 62 47 37
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1406 1675 1889 2191 2572 1406
Size: 269 214 302 381 3459
Fragments arranged by size:
3459 381 302 269 214
BsrBI CCG'CTC
Cuts at: 50 2917 2971 4455 4620 50
Size: 2867 54 1484 165 55
Fragments arranged by size:
2867 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2537 2537
Size: 4625
BsrFI r'CCGG_y
Cuts at: 551 716 1275 2623 2804 3013 551
Size: 165 559 1348 181 209 2163
Fragments arranged by size:
2163 1348 559 209 181 165
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 605 679 861 1312 1548 2002 605
Size: 74 182 451 236 454 3228
Fragments arranged by size:
3228 454 451 236 182 74
BstBI TT'CG_AA
Cuts at: 110 2986 110
Size: 2876 1749
BstEII G'GTnAC_C
Cuts at: 586 586
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 808 808
Size: 4625
BstYI r'GATC_y
Cuts at: 1 1159 1650 2117 2140 2475 2721 3733 3819
Size: 1158 491 467 23 335 246 1012 86
Cuts at: 3819 3831 4177 1
Size: 12 346 449
Fragments arranged by size:
1158 1012 491 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 884 1007 1085 884
Size: 123 78 4424
Fragments arranged by size:
4424 123 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 507 708 1306 1868 507
Size: 201 598 562 3264
Fragments arranged by size:
3264 598 562 201
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DraIII CAC_nnn'GTG
Cuts at: 756 756
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 392 563 810 2210 2384 2775 2802 4147 392
Size: 171 247 1400 174 391 27 1345 870
Fragments arranged by size:
1400 1345 870 391 247 174 171 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 838 929 1128 2648 2858 838
Size: 91 199 1520 210 2605
Fragments arranged by size:
2605 1520 210 199 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 1231 2833 3143 3289 4435 241
Size: 990 1602 310 146 1146 431
Fragments arranged by size:
1602 1146 990 431 310 146
EcoICRI GAG'CTC
Cuts at: 1637 1637
Size: 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 2450 2882 3629 3897 670
Size: 1780 432 747 268 1398
Fragments arranged by size:
1780 1398 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1336 1336
Size: 4625
EcoO109I rG'GnC_Cy
Cuts at: 501 633 1341 1764 501
Size: 132 708 423 3362
Fragments arranged by size:
3362 708 423 132
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1327 2644 1327
Size: 1317 3308
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 558 2059 2307 3329 4308 558
Size: 1501 248 1022 979 875
Fragments arranged by size:
1501 1022 979 875 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 471 503 1421 1453 2467 2497 2499 2529 471
Size: 32 918 32 1014 30 2 30 2567
Fragments arranged by size:
2567 1014 918 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1664 1664
Size: 4625
KasI G'GCGC_C
Cuts at: 554 2055 2303 554
Size: 1501 248 2876
Fragments arranged by size:
2876 1501 248
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 829 4058 829
Size: 3229 1396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3296 3480 3296
Size: 184 4441
MscI TGG'CCA
Cuts at: 394 565 2386 394
Size: 171 1821 2633
Fragments arranged by size:
2633 1821 171
MslI CAynn'nnrTG
Cuts at: 701 1012 1051 1090 2614 2741 701
Size: 311 39 39 1524 127 2585
Fragments arranged by size:
2585 1524 311 127 39 39
MspA1I CmG'CkG
Cuts at: 342 584 596 804 908 1249 1285 1736 2050
Size: 242 12 208 104 341 36 451 314
Cuts at: 2050 2410 3423 3668 342
Size: 360 1013 245 1299
Fragments arranged by size:
1299 1013 451 360 341 314 245 242
208 104 36 12
NaeI GCC'GGC
Cuts at: 1277 2806 1277
Size: 1529 3096
NarI GG'CG_CC
Cuts at: 555 2056 2304 555
Size: 1501 248 2876
Fragments arranged by size:
2876 1501 248
NcoI C'CATG_G
Cuts at: 1007 1085 1007
Size: 78 4547
NgoMIV G'CCGG_C
Cuts at: 1275 2804 1275
Size: 1529 3096
NsiI A_TGCA'T
Cuts at: 572 572
Size: 4625
NspI r_CATG'y
Cuts at: 574 894 1002 1017 1678 2178 3085 574
Size: 320 108 15 661 500 907 2114
Fragments arranged by size:
2114 907 661 500 320 108 15
PciI A'CATG_T
Cuts at: 890 3081 890
Size: 2191 2434
PflMI CCAn_nnn'nTGG
Cuts at: 1377 1513 1377
Size: 136 4489
PfoI T'CCnGG_A
Cuts at: 1224 1224
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PstI C_TGCA'G
Cuts at: 479 614 913 1672 2357 479
Size: 135 299 759 685 2747
Fragments arranged by size:
2747 759 685 299 135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 342 596 908 1285 2050 2410 342
Size: 254 312 377 765 360 2557
Fragments arranged by size:
2557 765 377 360 312 254
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1639 1639
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 838 929 2648 2858 838
Size: 91 1719 210 2605
Fragments arranged by size:
2605 1719 210 91
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 793 4142 793
Size: 3349 1276
SfcI C'TryA_G
Cuts at: 33 475 610 909 1668 2353 3346 3537 33
Size: 442 135 299 759 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 442 299 191 135
SfoI GGC'GCC
Cuts at: 556 2057 2305 556
Size: 1501 248 2876
Fragments arranged by size:
2876 1501 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 865 1496 1780 2695 3187 3449 865
Size: 631 284 915 492 262 2041
Fragments arranged by size:
2041 915 631 492 284 262
SnaBI TAC'GTA
Cuts at: 1617 1617
Size: 4625
SpeI A'CTAG_T
Cuts at: 67 198 67
Size: 131 4494
SphI G_CATG'C
Cuts at: 574 1678 2178 574
Size: 1104 500 3021
Fragments arranged by size:
3021 1104 500
StuI AGG'CCT
Cuts at: 210 294 899 988 1433 210
Size: 84 605 89 445 3402
Fragments arranged by size:
3402 605 445 89 84
StyI C'CwwG_G
Cuts at: 683 983 1007 1085 1759 2113 683
Size: 300 24 78 674 354 3195
Fragments arranged by size:
3195 674 354 300 78 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 412 2208 2341 2800 3009 412
Size: 1796 133 459 209 2028
Fragments arranged by size:
2028 1796 459 209 133
TatI w'GTAC_w
Cuts at: 428 791 4140 428
Size: 363 3349 913
Fragments arranged by size:
3349 913 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 769 801 4142 4174 769
Size: 32 3341 32 1220
Fragments arranged by size:
3341 1220 32 32
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1197 1431 2120 1197
Size: 234 689 3702
Fragments arranged by size:
3702 689 234
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BglI BmtI BpmI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI