Restriction map for
pF1KSDA0199
(Circular) (Six-base) MAPSORT of: pF1KSDA0199.seq Check: 8580 from: 1 to: 6974
pF1KSDA0199 6974 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2755 2755
Size: 6974
AatII G_ACGT'C
Cuts at: 1903 2243 1903
Size: 340 6634
AccI GT'mk_AC
Cuts at: 1244 1646 1721 3833 4012 1244
Size: 402 75 2112 179 4206
Fragments arranged by size:
4206 2112 402 179 75
Acc65I G'GTAC_C
Cuts at: 3990 3990
Size: 6974
AclI AA'CG_TT
Cuts at: 6814 6814
Size: 6974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1402 2019 2844 3454 4799 5231 5978 6246
Size: 677 617 825 610 1345 432 747 268
Cuts at: 6246 725
Size: 1453
Fragments arranged by size:
1453 1345 825 747 677 617 610 432
268
AflIII A'CryG_T
Cuts at: 4326 5430 6407 4326
Size: 1104 977 4893
Fragments arranged by size:
4893 1104 977
AgeI A'CCGG_T
Cuts at: 5362 5362
Size: 6974
AleI CACnn'nnGTG
Cuts at: 304 1354 3496 304
Size: 1050 2142 3782
Fragments arranged by size:
3782 2142 1050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 872 872
Size: 6974
AlwNI CAG_nnn'CTG
Cuts at: 286 1425 2159 3677 3723 3876 4279 5846 286
Size: 1139 734 1518 46 153 403 1567 1414
Fragments arranged by size:
1567 1518 1414 1139 734 403 153 46
ApaI G_GGCC'C
Cuts at: 2177 2215 2360 2707 3015 2177
Size: 38 145 347 308 6136
Fragments arranged by size:
6136 347 308 145 38
ApaLI G'TGCA_C
Cuts at: 946 3353 5744 946
Size: 2407 2391 2176
Fragments arranged by size:
2407 2391 2176
ApoI r'AATT_y
Cuts at: 292 3978 6327 6583 292
Size: 3686 2349 256 683
Fragments arranged by size:
3686 2349 683 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6974
AvaI C'yCGr_G
Cuts at: 161 485 2112 2359 2413 2699 2803 2837 2983
Size: 324 1627 247 54 286 104 34 146
Cuts at: 2983 3994 161
Size: 1011 3141
Fragments arranged by size:
3141 1627 1011 324 286 247 146 104
54 34
BamHI G'GATC_C
Cuts at: 3999 3999
Size: 6974
BanI G'GyrC_C
Cuts at: 385 1693 2135 2813 3990 4404 4652 4687 385
Size: 1308 442 678 1177 414 248 35 2672
Fragments arranged by size:
2672 1308 1177 678 442 414 248 35
BanII G_rGCy'C
Cuts at: 185 980 1780 1797 2177 2215 2360 2707 2810
Size: 795 800 17 380 38 145 347 103
Cuts at: 2810 3015 3923 3988 5018 185
Size: 205 908 65 1030 2141
Fragments arranged by size:
2141 1030 908 800 795 380 347 205
145 103 65 38 17
BbeI G_GCGC'C
Cuts at: 4408 4656 4408
Size: 248 6726
BbsI GAAGACnn'nnnn_
Cuts at: 834 1595 834
Size: 761 6213
BbvCI CC'TCA_GC
Cuts at: 1515 3244 1515
Size: 1729 5245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1116 1150 1734 1768 3045 3079 1116
Size: 34 584 34 1277 34 5011
Fragments arranged by size:
5011 1277 584 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4872 5633 6974 4872
Size: 761 1341 4872
Fragments arranged by size:
4872 1341 761
BclI T'GATC_A
Cuts at: 4494 4494
Size: 6974
BglI GCCn_nnn'nGGC
Cuts at: 394 2364 2510 2702 394
Size: 1970 146 192 4666
Fragments arranged by size:
4666 1970 192 146
BglII A'GATC_T
Cuts at: 4489 6526 4489
Size: 2037 4937
BlpI GC'TnA_GC
Cuts at: 974 1096 3922 4086 974
Size: 122 2826 164 3862
Fragments arranged by size:
3862 2826 164 122
Bme1580I G_kGCm'C
Cuts at: 390 885 950 1361 2177 2192 2215 2360 2707
Size: 495 65 411 816 15 23 145 347
Cuts at: 2707 3015 3350 3357 3941 4599 4692 5748 390
Size: 308 335 7 584 658 93 1056 1616
Fragments arranged by size:
1616 1056 816 658 584 495 411 347
335 308 145 93 65 23 15 7
BmgBI CAC'GTC
Cuts at: 2517 2517
Size: 6974
BmrI ACTGGGnnnn_n'
Cuts at: 678 1951 2086 2202 3011 3416 4186 4267 4602
Size: 1273 135 116 809 405 770 81 335
Cuts at: 4602 6746 678
Size: 2144 906
Fragments arranged by size:
2144 1273 906 809 770 405 335 135
116 81
BmtI G_CTAG'C
Cuts at: 1474 1474
Size: 6974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2138 2994 3009 3159 3162 5341 2138
Size: 856 15 150 3 2179 3771
Fragments arranged by size:
3771 2179 856 150 15 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2146 3057 3112 3281 3463 3970 1270
Size: 876 911 55 169 182 507 4274
Fragments arranged by size:
4274 911 876 507 182 169 55
Bpu10I CC'TnA_GC
Cuts at: 604 620 1279 1515 1880 1919 2615 3244 604
Size: 16 659 236 365 39 696 629 4334
Fragments arranged by size:
4334 696 659 629 365 236 39 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2629 4150 5029 5521 5819 2629
Size: 1521 879 492 298 3784
Fragments arranged by size:
3784 1521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 825 1236 3147 3223 6678 825
Size: 411 1911 76 3455 1121
Fragments arranged by size:
3455 1911 1121 411 76
BsaAI yAC'GTr
Cuts at: 522 945 3966 4327 522
Size: 423 3021 361 3169
Fragments arranged by size:
3169 3021 423 361
BsaBI GATnn'nnATC
Cuts at: 6 1711 4488 6525 6
Size: 1705 2777 2037 455
Fragments arranged by size:
2777 2037 1705 455
BsaHI Gr'CG_yC
Cuts at: 1144 1900 2240 3104 4405 4653 5355 6851 1144
Size: 756 340 864 1301 248 702 1496 1267
Fragments arranged by size:
1496 1301 1267 864 756 702 340 248
BsaWI w'CCGG_w
Cuts at: 512 4163 4684 5362 5636 5783 6194 512
Size: 3651 521 678 274 147 411 1292
Fragments arranged by size:
3651 1292 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 3436 3466 547
Size: 30 2859 30 4055
Fragments arranged by size:
4055 2859 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 520 571 973 2677 2905 3135 3151 175
Size: 345 51 402 1704 228 230 16 3998
Fragments arranged by size:
3998 1704 402 345 230 228 51 16
BseYI C'CCAG_C
Cuts at: 904 1323 1731 1834 2185 2265 2346 2624 3391
Size: 419 408 103 351 80 81 278 767
Cuts at: 3391 3857 4399 5157 5734 6230 904
Size: 466 542 758 577 496 1648
Fragments arranged by size:
1648 767 758 577 542 496 466 419
408 351 278 103 81 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1110 2245 2893 2895 3176 883
Size: 227 1135 648 2 281 4681
Fragments arranged by size:
4681 1135 648 281 227 2
BsiEI CG_ry'CG
Cuts at: 104 2151 2906 3087 3497 4516 4562 5770 104
Size: 2047 755 181 410 1019 46 1208 1308
Fragments arranged by size:
2047 1308 1208 1019 755 410 181 46
BsiHKAI G_wGCw'C
Cuts at: 950 980 1097 1217 3357 3923 3988 4766 4956
Size: 30 117 120 2140 566 65 778 190
Cuts at: 4956 5748 950
Size: 792 2176
Fragments arranged by size:
2176 2140 792 778 566 190 120 117
65 30
BsmI GAATG_Cn'
Cuts at: 490 2558 490
Size: 2068 4906
BsmBI CGTCTCn'nnnn_
Cuts at: 2231 2410 2231
Size: 179 6795
Bsp1286I G_dGCh'C
Cuts at: 185 390 885 950 980 1097 1217 1361 1780
Size: 205 495 65 30 117 120 144 419
Cuts at: 1780 1797 2177 2192 2215 2360 2707 2810 3015
Size: 17 380 15 23 145 347 103 205
Cuts at: 3015 3350 3357 3923 3941 3988 4599 4692 4766
Size: 335 7 566 18 47 611 93 74
Cuts at: 4766 4956 5018 5748 185
Size: 190 62 730 1411
Fragments arranged by size:
1411 730 611 566 495 419 380 347
335 205 205 190 145 144 120 117
103 93 74 65 62 47 30 23
18 17 15 7
BspEI T'CCGG_A
Cuts at: 512 6194 512
Size: 5682 1292
BspHI T'CATG_A
Cuts at: 1301 6150 1301
Size: 4849 2125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 1625 2190 2755 3770 4024 4238 4540 4921
Size: 1035 565 565 1015 254 214 302 381
Cuts at: 4921 590
Size: 2643
Fragments arranged by size:
2643 1035 1015 565 565 381 302 254
214
BsrBI CCG'CTC
Cuts at: 50 1551 2834 3160 5266 5320 6804 6969 50
Size: 1501 1283 326 2106 54 1484 165 55
Fragments arranged by size:
2106 1501 1484 1283 326 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4886 4886
Size: 6974
BsrFI r'CCGG_y
Cuts at: 2132 2278 2943 3086 4972 5153 5362 2132
Size: 146 665 143 1886 181 209 3744
Fragments arranged by size:
3744 1886 665 209 181 146 143
BsrGI T'GTAC_A
Cuts at: 2249 3302 3500 2249
Size: 1053 198 5723
Fragments arranged by size:
5723 1053 198
BssSI C'ACGA_G
Cuts at: 718 3450 5245 5603 718
Size: 2732 1795 358 2089
Fragments arranged by size:
2732 2089 1795 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4351 4351
Size: 6974
BstBI TT'CG_AA
Cuts at: 110 5335 110
Size: 5225 1749
BstEII G'GTnAC_C
Cuts at: 1968 1968
Size: 6974
BstXI CCAn_nnnn'nTGG
Cuts at: 837 867 1227 1323 1527 3522 837
Size: 30 360 96 204 1995 4289
Fragments arranged by size:
4289 1995 360 204 96 30
BstYI r'GATC_y
Cuts at: 1 509 3872 3999 4466 4489 4824 5070 6082
Size: 508 3363 127 467 23 335 246 1012
Cuts at: 6082 6168 6180 6526 1
Size: 86 12 346 449
Fragments arranged by size:
3363 1012 508 467 449 346 335 246
127 86 23 12
BstZ17I GTA'TAC
Cuts at: 1722 1722
Size: 6974
Bsu36I CC'TnA_GG
Cuts at: 595 1499 1982 2141 2963 3147 595
Size: 904 483 159 822 184 4422
Fragments arranged by size:
4422 904 822 483 184 159
BtgI C'CryG_G
Cuts at: 177 1082 1316 2232 3515 3603 177
Size: 905 234 916 1283 88 3548
Fragments arranged by size:
3548 1283 916 905 234 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4924 5105 5268 4924
Size: 181 163 6630
Fragments arranged by size:
6630 181 163
BtsI GCAGTG_nn'
Cuts at: 253 2160 3236 3657 3921 4217 253
Size: 1907 1076 421 264 296 3010
Fragments arranged by size:
3010 1907 1076 421 296 264
DraI TTT'AAA
Cuts at: 3973 3973
Size: 6974
DraIII CAC_nnn'GTG
Cuts at: 3345 3345
Size: 6974
DrdI GACnn_nn'nnGTC
Cuts at: 1957 4680 5538 1957
Size: 2723 858 3393
Fragments arranged by size:
3393 2723 858
EaeI y'GGCC_r
Cuts at: 1313 2023 2057 2148 2273 2445 2471 2829 2903
Size: 710 34 91 125 172 26 358 74
Cuts at: 2903 3084 3524 3777 4559 4733 5124 5151 6496
Size: 181 440 253 782 174 391 27 1345
Cuts at: 6496 1313
Size: 1791
Fragments arranged by size:
1791 1345 782 710 440 391 358 253
181 174 172 125 91 74 34 27
26
EagI C'GGCC_G
Cuts at: 2148 2903 3084 4559 2148
Size: 755 181 1475 4563
Fragments arranged by size:
4563 1475 755 181
EarI CTCTTCn'nnn_
Cuts at: 223 805 1138 2038 2728 3466 4997 5207 223
Size: 582 333 900 690 738 1531 210 1990
Fragments arranged by size:
1990 1531 900 738 690 582 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 943 2005 2092 2382 2699 3090 5182 5492 5638
Size: 1062 87 290 317 391 2092 310 146
Cuts at: 5638 6784 943
Size: 1146 1133
Fragments arranged by size:
2092 1146 1133 1062 391 317 310 290
146 87
EcoICRI GAG'CTC
Cuts at: 978 3921 3986 978
Size: 2943 65 3966
Fragments arranged by size:
3966 2943 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1270 1402 2019 2146 2844 3057 3112 3281
Size: 545 132 617 127 698 213 55 169
Cuts at: 3281 3454 3463 3970 4799 5231 5978 6246 725
Size: 173 9 507 829 432 747 268 1453
Fragments arranged by size:
1453 829 747 698 617 545 507 432
268 213 173 169 132 127 55 9
EcoO109I rG'GnC_Cy
Cuts at: 283 1978 2173 2203 2211 2703 2704 2958 3012
Size: 1695 195 30 8 492 1 254 54
Cuts at: 3012 3266 3720 3801 3816 4113 283
Size: 254 454 81 15 297 3144
Fragments arranged by size:
3144 1695 492 454 297 254 254 195
81 54 30 15 8 1
EcoRI G'AATT_C
Cuts at: 292 3978 292
Size: 3686 3288
EcoRV GAT'ATC
Cuts at: 158 158
Size: 6974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4993 4993
Size: 6974
FspI TGC'GCA
Cuts at: 1744 4755 1744
Size: 3011 3963
HaeII r_GCGC'y
Cuts at: 1680 2266 2583 3399 4408 4656 5678 6657 1680
Size: 586 317 816 1009 248 1022 979 1997
Fragments arranged by size:
1997 1022 1009 979 816 586 317 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1279 1311 3205 3237 3601 3633 4816
Size: 32 1105 32 1894 32 364 32 1183
Cuts at: 4816 4846 4848 4878 142
Size: 30 2 30 2238
Fragments arranged by size:
2238 1894 1183 1105 364 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4013 4013
Size: 6974
HindIII A'AGCT_T
Cuts at: 3738 3738
Size: 6974
KasI G'GCGC_C
Cuts at: 4404 4652 4404
Size: 248 6726
KpnI G_GTAC'C
Cuts at: 3994 3994
Size: 6974
MluI A'CGCG_T
Cuts at: 6407 6407
Size: 6974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 519 5645 5829 519
Size: 5126 184 1664
Fragments arranged by size:
5126 1664 184
MscI TGG'CCA
Cuts at: 1315 2059 2275 2473 3526 4735 1315
Size: 744 216 198 1053 1209 3554
Fragments arranged by size:
3554 1209 1053 744 216 198
MslI CAynn'nnrTG
Cuts at: 304 854 1114 1354 1810 3067 3496 4963 5090
Size: 550 260 240 456 1257 429 1467 127
Cuts at: 5090 304
Size: 2188
Fragments arranged by size:
2188 1467 1257 550 456 429 260 240
127
MspA1I CmG'CkG
Cuts at: 788 1557 2346 2603 2864 2877 3083 3388 4085
Size: 769 789 257 261 13 206 305 697
Cuts at: 4085 4399 4759 5772 6017 788
Size: 314 360 1013 245 1745
Fragments arranged by size:
1745 1013 789 769 697 360 314 305
261 257 245 206 13
NaeI GCC'GGC
Cuts at: 2134 3088 5155 2134
Size: 954 2067 3953
Fragments arranged by size:
3953 2067 954
NarI GG'CG_CC
Cuts at: 4405 4653 4405
Size: 248 6726
NcoI C'CATG_G
Cuts at: 177 2232 3515 177
Size: 2055 1283 3636
Fragments arranged by size:
3636 2055 1283
NgoMIV G'CCGG_C
Cuts at: 2132 3086 5153 2132
Size: 954 2067 3953
Fragments arranged by size:
3953 2067 954
NheI G'CTAG_C
Cuts at: 1470 1470
Size: 6974
NspI r_CATG'y
Cuts at: 1827 2487 4027 4527 5434 1827
Size: 660 1540 500 907 3367
Fragments arranged by size:
3367 1540 907 660 500
PasI CC'CwG_GG
Cuts at: 646 3271 646
Size: 2625 4349
PciI A'CATG_T
Cuts at: 5430 5430
Size: 6974
PflMI CCAn_nnn'nTGG
Cuts at: 501 2454 3516 3876 501
Size: 1953 1062 360 3599
Fragments arranged by size:
3599 1953 1062 360
PfoI T'CCnGG_A
Cuts at: 1367 1367
Size: 6974
PmeI GTTT'AAAC
Cuts at: 3973 3973
Size: 6974
PmlI CAC'GTG
Cuts at: 522 945 522
Size: 423 6551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6113 6145 6113
Size: 32 6942
PpuMI rG'GwC_Cy
Cuts at: 283 2203 3266 3720 3801 3816 283
Size: 1920 1063 454 81 15 3441
Fragments arranged by size:
3441 1920 1063 454 81 15
PspOMI G'GGCC_C
Cuts at: 2173 2211 2356 2703 3011 2173
Size: 38 145 347 308 6136
Fragments arranged by size:
6136 347 308 145 38
PstI C_TGCA'G
Cuts at: 737 919 3053 3130 3257 4021 4706 737
Size: 182 2134 77 127 764 685 3005
Fragments arranged by size:
3005 2134 764 685 182 127 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6974
PvuII CAG'CTG
Cuts at: 1557 2346 2864 4399 4759 1557
Size: 789 518 1535 360 3772
Fragments arranged by size:
3772 1535 789 518 360
RsrII CG'GwC_CG
Cuts at: 5169 5169
Size: 6974
SacI G_AGCT'C
Cuts at: 980 3923 3988 980
Size: 2943 65 3966
Fragments arranged by size:
3966 2943 65
SalI G'TCGA_C
Cuts at: 4011 4011
Size: 6974
SanDI GG'GwC_CC
Cuts at: 3266 3266
Size: 6974
SapI GCTCTTCn'nnn_
Cuts at: 2038 4997 5207 2038
Size: 2959 210 3805
Fragments arranged by size:
3805 2959 210
SbfI CC_TGCA'GG
Cuts at: 4021 4021
Size: 6974
ScaI AGT'ACT
Cuts at: 6491 6491
Size: 6974
SfcI C'TryA_G
Cuts at: 33 733 915 3049 3126 3253 4017 4702 5695
Size: 700 182 2134 77 127 764 685 993
Cuts at: 5695 5886 33
Size: 191 1121
Fragments arranged by size:
2134 1121 993 764 700 685 191 182
127 77
SfoI GGC'GCC
Cuts at: 4406 4654 4406
Size: 248 6726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6974
SmaI CCC'GGG
Cuts at: 487 2361 2701 3996 487
Size: 1874 340 1295 3465
Fragments arranged by size:
3465 1874 1295 340
SmlI C'TyrA_G
Cuts at: 2608 4129 5044 5536 5798 2608
Size: 1521 915 492 262 3784
Fragments arranged by size:
3784 1521 915 492 262
SnaBI TAC'GTA
Cuts at: 3966 3966
Size: 6974
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6974
SphI G_CATG'C
Cuts at: 1827 2487 4027 4527 1827
Size: 660 1540 500 4274
Fragments arranged by size:
4274 1540 660 500
SrfI GCCC'GGGC
Cuts at: 2361 2361
Size: 6974
StuI AGG'CCT
Cuts at: 593 1277 1504 593
Size: 684 227 6063
Fragments arranged by size:
6063 684 227
StyI C'CwwG_G
Cuts at: 177 1272 2207 2232 3224 3515 4108 4462 177
Size: 1095 935 25 992 291 593 354 2689
Fragments arranged by size:
2689 1095 992 935 593 354 291 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 373 592 1774 2338 2771 3733 4557 4690 5149
Size: 219 1182 564 433 962 824 133 459
Cuts at: 5149 5358 373
Size: 209 1989
Fragments arranged by size:
1989 1182 962 824 564 459 433 219
209 133
TatI w'GTAC_w
Cuts at: 782 2249 3302 3500 3564 6489 782
Size: 1467 1053 198 64 2925 1267
Fragments arranged by size:
2925 1467 1267 1053 198 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 547 579 2449 2481 3436 3468 3834 3866 6491
Size: 32 1870 32 955 32 366 32 2625
Cuts at: 6491 6523 547
Size: 32 998
Fragments arranged by size:
2625 1870 998 955 366 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1048 4771 1048
Size: 3723 3251
XbaI T'CTAG_A
Cuts at: 4005 4005
Size: 6974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 813 1065 1970 3039 4469 813
Size: 252 905 1069 1430 3318
Fragments arranged by size:
3318 1430 1069 905 252
XmaI C'CCGG_G
Cuts at: 485 2359 2699 3994 485
Size: 1874 340 1295 3465
Fragments arranged by size:
3465 1874 1295 340
ZraI GAC'GTC
Cuts at: 1901 2241 1901
Size: 340 6634
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BfrBI
BsiWI BssHII ClaI CspCI EcoNI FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SspI SwaI
XhoI XmnI