Restriction map for
pF1KSDA0200
(Circular) (Six-base) MAPSORT of: pF1KSDA0200.seq Check: 8255 from: 1 to: 6185
pF1KSDA0200 6185 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 148 241 1249 148
Size: 93 1008 5084
Fragments arranged by size:
5084 1008 93
AccI GT'mk_AC
Cuts at: 3223 3223
Size: 6185
Acc65I G'GTAC_C
Cuts at: 3201 3201
Size: 6185
AclI AA'CG_TT
Cuts at: 6025 6025
Size: 6185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 2050 2288 2931 3113 3136 4010 4442 5189
Size: 1257 238 643 182 23 874 432 747
Cuts at: 5189 5457 793
Size: 268 1521
Fragments arranged by size:
1521 1257 874 747 643 432 268 238
182 23
AfeI AGC'GCT
Cuts at: 310 310
Size: 6185
AflIII A'CryG_T
Cuts at: 3537 4641 5618 3537
Size: 1104 977 4104
Fragments arranged by size:
4104 1104 977
AgeI A'CCGG_T
Cuts at: 2595 4573 2595
Size: 1978 4207
AhdI GACnn_n'nnGTC
Cuts at: 969 969
Size: 6185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 862 1349 862
Size: 487 5698
AlwNI CAG_nnn'CTG
Cuts at: 233 1764 2815 2932 3033 3490 5057 233
Size: 1531 1051 117 101 457 1567 1361
Fragments arranged by size:
1567 1531 1361 1051 457 117 101
ApaI G_GGCC'C
Cuts at: 1285 1430 2293 1285
Size: 145 863 5177
Fragments arranged by size:
5177 863 145
ApaLI G'TGCA_C
Cuts at: 2846 4955 2846
Size: 2109 4076
ApoI r'AATT_y
Cuts at: 1027 2631 3189 5538 5794 1027
Size: 1604 558 2349 256 1418
Fragments arranged by size:
2349 1604 1418 558 256
AseI AT'TA_AT
Cuts at: 20 1013 20
Size: 993 5192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6185
AvaI C'yCGr_G
Cuts at: 262 418 1187 1395 1713 2252 3205 262
Size: 156 769 208 318 539 953 3242
Fragments arranged by size:
3242 953 769 539 318 208 156
AvrII C'CTAG_G
Cuts at: 1146 1146
Size: 6185
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2125 2158 2125
Size: 33 6152
BamHI G'GATC_C
Cuts at: 3210 3210
Size: 6185
BanI G'GyrC_C
Cuts at: 218 375 909 1386 3201 3615 3863 3898 218
Size: 157 534 477 1815 414 248 35 2505
Fragments arranged by size:
2505 1815 534 477 414 248 157 35
BanII G_rGCy'C
Cuts at: 887 1156 1254 1285 1430 2293 2809 2923 2959
Size: 269 98 31 145 863 516 114 36
Cuts at: 2959 3074 3199 4229 887
Size: 115 125 1030 2843
Fragments arranged by size:
2843 1030 863 516 269 145 125 115
114 98 36 31
BbeI G_GCGC'C
Cuts at: 222 379 3619 3867 222
Size: 157 3240 248 2540
Fragments arranged by size:
3240 2540 248 157
BbsI GAAGACnn'nnnn_
Cuts at: 547 797 926 965 547
Size: 250 129 39 5767
Fragments arranged by size:
5767 250 129 39
BbvCI CC'TCA_GC
Cuts at: 2897 2897
Size: 6185
BciVI GTATCCnnnnn_n'
Cuts at: 4083 4844 6185 4083
Size: 761 1341 4083
Fragments arranged by size:
4083 1341 761
BclI T'GATC_A
Cuts at: 806 3705 806
Size: 2899 3286
BglI GCCn_nnn'nGGC
Cuts at: 367 388 1151 1384 1744 2396 2787 367
Size: 21 763 233 360 652 391 3765
Fragments arranged by size:
3765 763 652 391 360 233 21
BglII A'GATC_T
Cuts at: 3700 5737 3700
Size: 2037 4148
BlpI GC'TnA_GC
Cuts at: 2808 3297 2808
Size: 489 5696
Bme1580I G_kGCm'C
Cuts at: 914 1285 1430 2213 2293 2783 2850 3810 3903
Size: 371 145 783 80 490 67 960 93
Cuts at: 3903 4959 914
Size: 1056 2140
Fragments arranged by size:
2140 1056 960 783 490 371 145 93
80 67
BmrI ACTGGGnnnn_n'
Cuts at: 731 821 1196 1268 2133 2833 3397 3478 3813
Size: 90 375 72 865 700 564 81 335
Cuts at: 3813 5957 731
Size: 2144 959
Fragments arranged by size:
2144 959 865 700 564 375 335 90
81 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1323 1565 1806 4552 1323
Size: 242 241 2746 2956
Fragments arranged by size:
2956 2746 242 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 298 578 650 690 1019 1306 1345 1555
Size: 6 280 72 40 329 287 39 210
Cuts at: 1555 2226 2700 292
Size: 671 474 3777
Fragments arranged by size:
3777 671 474 329 287 280 210 72
40 39 6
Bpu10I CC'TnA_GC
Cuts at: 2897 2917 2933 2897
Size: 20 16 6149
Fragments arranged by size:
6149 20 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3361 4240 4732 5030 3361
Size: 879 492 298 4516
Fragments arranged by size:
4516 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3173 5889 3173
Size: 2716 3469
BsaAI yAC'GTr
Cuts at: 3177 3538 3177
Size: 361 5824
BsaBI GATnn'nnATC
Cuts at: 6 3699 5736 6
Size: 3693 2037 455
Fragments arranged by size:
3693 2037 455
BsaHI Gr'CG_yC
Cuts at: 219 376 404 638 3616 3864 4566 6062 219
Size: 157 28 234 2978 248 702 1496 342
Fragments arranged by size:
2978 1496 702 342 248 234 157 28
BsaWI w'CCGG_w
Cuts at: 2595 3374 3895 4573 4847 4994 5405 2595
Size: 779 521 678 274 147 411 3375
Fragments arranged by size:
3375 779 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1933 1963 1933
Size: 30 6155
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 907 967 3148 907
Size: 60 2181 3944
Fragments arranged by size:
3944 2181 60
BseYI C'CCAG_C
Cuts at: 1141 1576 1757 1910 2269 2429 2549 2874 2922
Size: 435 181 153 359 160 120 325 48
Cuts at: 2922 2937 3610 4368 4945 5441 1141
Size: 15 673 758 577 496 1885
Fragments arranged by size:
1885 758 673 577 496 435 359 325
181 160 153 120 48 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 2974 2979 286
Size: 2688 5 3492
Fragments arranged by size:
3492 2688 5
BsiEI CG_ry'CG
Cuts at: 104 429 2979 3727 3773 4981 104
Size: 325 2550 748 46 1208 1308
Fragments arranged by size:
2550 1308 1208 748 325 46
BsiHKAI G_wGCw'C
Cuts at: 425 887 2809 2850 3199 3977 4167 4959 425
Size: 462 1922 41 349 778 190 792 1651
Fragments arranged by size:
1922 1651 792 778 462 349 190 41
BsmI GAATG_Cn'
Cuts at: 2660 2660
Size: 6185
BsmBI CGTCTCn'nnnn_
Cuts at: 2508 2508
Size: 6185
Bsp1286I G_dGCh'C
Cuts at: 425 887 914 1156 1254 1285 1430 2213 2293
Size: 462 27 242 98 31 145 783 80
Cuts at: 2293 2783 2809 2850 2923 2959 3074 3199 3810
Size: 490 26 41 73 36 115 125 611
Cuts at: 3810 3903 3977 4167 4229 4959 425
Size: 93 74 190 62 730 1651
Fragments arranged by size:
1651 783 730 611 490 462 242 190
145 125 115 98 93 80 74 73
62 41 36 31 27 26
BspEI T'CCGG_A
Cuts at: 5405 5405
Size: 6185
BspHI T'CATG_A
Cuts at: 447 1520 5361 447
Size: 1073 3841 1271
Fragments arranged by size:
3841 1271 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 148 241 803 1249 1598 2166 3235 3449 3751
Size: 93 562 446 349 568 1069 214 302
Cuts at: 3751 4132 148
Size: 381 2201
Fragments arranged by size:
2201 1069 568 562 446 381 349 302
214 93
BsrBI CCG'CTC
Cuts at: 50 4477 4531 6015 6180 50
Size: 4427 54 1484 165 55
Fragments arranged by size:
4427 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4097 4097
Size: 6185
BsrFI r'CCGG_y
Cuts at: 197 215 1218 2595 4183 4364 4573 197
Size: 18 1003 1377 1588 181 209 1809
Fragments arranged by size:
1809 1588 1377 1003 209 181 18
BssHII G'CGCG_C
Cuts at: 333 333
Size: 6185
BssSI C'ACGA_G
Cuts at: 1780 4456 4814 1780
Size: 2676 358 3151
Fragments arranged by size:
3151 2676 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2001 2907 3562 2001
Size: 906 655 4624
Fragments arranged by size:
4624 906 655
BstBI TT'CG_AA
Cuts at: 110 4546 110
Size: 4436 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1177
Size: 6185
BstYI r'GATC_y
Cuts at: 1 3210 3677 3700 4035 4281 5293 5379 5391
Size: 3209 467 23 335 246 1012 86 12
Cuts at: 5391 5737 1
Size: 346 449
Fragments arranged by size:
3209 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 2264 2264
Size: 6185
BtgI C'CryG_G
Cuts at: 125 146 166 365 1460 1928 2470 2618 2792
Size: 21 20 199 1095 468 542 148 174
Cuts at: 2792 2819 3020 125
Size: 27 201 3290
Fragments arranged by size:
3290 1095 542 468 201 199 174 148
27 21 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4135 4316 4479 4135
Size: 181 163 5841
Fragments arranged by size:
5841 181 163
BtsI GCAGTG_nn'
Cuts at: 2705 2783 2991 3072 3428 2705
Size: 78 208 81 356 5462
Fragments arranged by size:
5462 356 208 81 78
DraI TTT'AAA
Cuts at: 3184 3184
Size: 6185
DrdI GACnn_nn'nnGTC
Cuts at: 3891 4749 3891
Size: 858 5327
EaeI y'GGCC_r
Cuts at: 426 434 1722 1851 1931 2670 3770 3944 4335
Size: 8 1288 129 80 739 1100 174 391
Cuts at: 4335 4362 5707 426
Size: 27 1345 904
Fragments arranged by size:
1345 1288 1100 904 739 391 174 129
80 27 8
EagI C'GGCC_G
Cuts at: 426 3770 426
Size: 3344 2841
EarI CTCTTCn'nnn_
Cuts at: 582 4208 4418 582
Size: 3626 210 2349
Fragments arranged by size:
3626 2349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 1207 4393 4703 4849 5995 166
Size: 1041 3186 310 146 1146 356
Fragments arranged by size:
3186 1146 1041 356 310 146
EcoICRI GAG'CTC
Cuts at: 885 2807 3197 885
Size: 1922 390 3873
Fragments arranged by size:
3873 1922 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 298 578 650 690 793 1019 1306 1345
Size: 6 280 72 40 103 226 287 39
Cuts at: 1345 1555 2050 2226 2288 2700 2931 3113 3136
Size: 210 495 176 62 412 231 182 23
Cuts at: 3136 4010 4442 5189 5457 292
Size: 874 432 747 268 1020
Fragments arranged by size:
1020 874 747 495 432 412 287 280
268 231 226 210 182 176 103 72
62 40 39 23 6
EcoO109I rG'GnC_Cy
Cuts at: 519 555 930 963 1108 1281 1292 1426 1427
Size: 36 375 33 145 173 11 134 1
Cuts at: 1427 1561 1907 2195 2290 2567 2825 2929 2942
Size: 134 346 288 95 277 258 104 13
Cuts at: 2942 3324 519
Size: 382 3380
Fragments arranged by size:
3380 382 375 346 288 277 258 173
145 134 134 104 95 36 33 13
11 1
EcoRI G'AATT_C
Cuts at: 1027 2631 3189 1027
Size: 1604 558 4023
Fragments arranged by size:
4023 1604 558
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1515 4204 1515
Size: 2689 3496
FspI TGC'GCA
Cuts at: 3966 3966
Size: 6185
HaeII r_GCGC'y
Cuts at: 192 222 270 285 312 379 479 2118 2867
Size: 30 48 15 27 67 100 1639 749
Cuts at: 2867 3619 3867 4889 5868 192
Size: 752 248 1022 979 509
Fragments arranged by size:
1639 1022 979 752 749 509 248 100
67 48 30 27 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 557 589 955 987 1734 1766 2961
Size: 32 284 32 366 32 747 32 1195
Cuts at: 2961 2993 4027 4057 4059 4089 241
Size: 32 1034 30 2 30 2337
Fragments arranged by size:
2337 1195 1034 747 366 284 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3224 3224
Size: 6185
KasI G'GCGC_C
Cuts at: 218 375 3615 3863 218
Size: 157 3240 248 2540
Fragments arranged by size:
3240 2540 248 157
KpnI G_GTAC'C
Cuts at: 3205 3205
Size: 6185
MluI A'CGCG_T
Cuts at: 5618 5618
Size: 6185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2160 2261 4856 5040 2160
Size: 101 2595 184 3305
Fragments arranged by size:
3305 2595 184 101
MscI TGG'CCA
Cuts at: 1724 1853 1933 2672 3946 1724
Size: 129 80 739 1274 3963
Fragments arranged by size:
3963 1274 739 129 80
MslI CAynn'nnrTG
Cuts at: 446 719 4174 4301 446
Size: 273 3455 127 2330
Fragments arranged by size:
3455 2330 273 127
MspA1I CmG'CkG
Cuts at: 168 409 1764 1981 2246 2509 2815 3022 3296
Size: 241 1355 217 265 263 306 207 274
Cuts at: 3296 3610 3970 4983 5228 168
Size: 314 360 1013 245 1125
Fragments arranged by size:
1355 1125 1013 360 314 306 274 265
263 245 241 217 207
NaeI GCC'GGC
Cuts at: 199 217 1220 4366 199
Size: 18 1003 3146 2018
Fragments arranged by size:
3146 2018 1003 18
NarI GG'CG_CC
Cuts at: 219 376 3616 3864 219
Size: 157 3240 248 2540
Fragments arranged by size:
3240 2540 248 157
NcoI C'CATG_G
Cuts at: 125 146 1460 2618 2792 125
Size: 21 1314 1158 174 3518
Fragments arranged by size:
3518 1314 1158 174 21
NgoMIV G'CCGG_C
Cuts at: 197 215 1218 4364 197
Size: 18 1003 3146 2018
Fragments arranged by size:
3146 2018 1003 18
NspI r_CATG'y
Cuts at: 2302 2379 2461 2730 3238 3738 4645 2302
Size: 77 82 269 508 500 907 3842
Fragments arranged by size:
3842 907 508 500 269 82 77
PasI CC'CwG_GG
Cuts at: 2680 2680
Size: 6185
PciI A'CATG_T
Cuts at: 4641 4641
Size: 6185
PmeI GTTT'AAAC
Cuts at: 3184 3184
Size: 6185
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5324 5356 5324
Size: 32 6153
PpuMI rG'GwC_Cy
Cuts at: 519 930 963 1907 2195 2567 2825 519
Size: 411 33 944 288 372 258 3879
Fragments arranged by size:
3879 944 411 372 288 258 33
PspOMI G'GGCC_C
Cuts at: 1281 1426 2289 1281
Size: 145 863 5177
Fragments arranged by size:
5177 863 145
PspXI vC'TCGA_Gb
Cuts at: 2252 2252
Size: 6185
PstI C_TGCA'G
Cuts at: 647 1092 2712 3232 3917 647
Size: 445 1620 520 685 2915
Fragments arranged by size:
2915 1620 685 520 445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6185
PvuII CAG'CTG
Cuts at: 1764 2246 2509 2815 3610 3970 1764
Size: 482 263 306 795 360 3979
Fragments arranged by size:
3979 795 482 360 306 263
RsrII CG'GwC_CG
Cuts at: 3017 4380 3017
Size: 1363 4822
SacI G_AGCT'C
Cuts at: 887 2809 3199 887
Size: 1922 390 3873
Fragments arranged by size:
3873 1922 390
SacII CC_GC'GG
Cuts at: 169 3023 169
Size: 2854 3331
SalI G'TCGA_C
Cuts at: 3222 3222
Size: 6185
SanDI GG'GwC_CC
Cuts at: 1907 2567 1907
Size: 660 5525
SapI GCTCTTCn'nnn_
Cuts at: 4208 4418 4208
Size: 210 5975
SbfI CC_TGCA'GG
Cuts at: 3232 3232
Size: 6185
ScaI AGT'ACT
Cuts at: 5702 5702
Size: 6185
SfcI C'TryA_G
Cuts at: 33 643 1088 2708 3045 3228 3913 4906 5097
Size: 610 445 1620 337 183 685 993 191
Cuts at: 5097 33
Size: 1121
Fragments arranged by size:
1620 1121 993 685 610 445 337 191
183
SfoI GGC'GCC
Cuts at: 220 377 3617 3865 220
Size: 157 3240 248 2540
Fragments arranged by size:
3240 2540 248 157
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6185
SgrAI Cr'CCGG_yG
Cuts at: 197 197
Size: 6185
SmaI CCC'GGG
Cuts at: 1189 1397 1715 3207 1189
Size: 208 318 1492 4167
Fragments arranged by size:
4167 1492 318 208
SmlI C'TyrA_G
Cuts at: 2252 3340 4255 4747 5009 2252
Size: 1088 915 492 262 3428
Fragments arranged by size:
3428 1088 915 492 262
SnaBI TAC'GTA
Cuts at: 3177 3177
Size: 6185
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6185
SphI G_CATG'C
Cuts at: 3238 3738 3238
Size: 500 5685
SspI AAT'ATT
Cuts at: 1017 1017
Size: 6185
StuI AGG'CCT
Cuts at: 3004 3004
Size: 6185
StyI C'CwwG_G
Cuts at: 125 146 323 998 1146 1460 2225 2602 2618
Size: 21 177 675 148 314 765 377 16
Cuts at: 2618 2792 3319 3673 125
Size: 174 527 354 2637
Fragments arranged by size:
2637 765 675 527 377 354 314 177
174 148 21 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 598 3013 3768 3901 4360 4569 598
Size: 2415 755 133 459 209 2214
Fragments arranged by size:
2415 2214 755 459 209 133
TatI w'GTAC_w
Cuts at: 1129 5700 1129
Size: 4571 1614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1933 1965 2214 2246 5702 5734 1933
Size: 32 249 32 3456 32 2384
Fragments arranged by size:
3456 2384 249 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3982 3982
Size: 6185
XbaI T'CTAG_A
Cuts at: 3216 3216
Size: 6185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 2790 3680 144
Size: 2646 890 2649
Fragments arranged by size:
2649 2646 890
XhoI C'TCGA_G
Cuts at: 2252 2252
Size: 6185
XmaI C'CCGG_G
Cuts at: 1187 1395 1713 3205 1187
Size: 208 318 1492 4167
Fragments arranged by size:
4167 1492 318 208
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI BcgI BfrBI BmgBI
BmtI BsiWI BsrGI BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PsrI SexAI SfiI SrfI SwaI XmnI
ZraI