Restriction map for
pF1KSDA0207
(Circular) (Six-base) MAPSORT of: pF1KSDA0207.seq Check: 4958 from: 1 to: 4903
pF1KSDA0207 4903 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1112 1112
Size: 4903
AccI GT'mk_AC
Cuts at: 1939 1939
Size: 4903
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4903
AclI AA'CG_TT
Cuts at: 4743 4743
Size: 4903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 460 1755 2726 3158 3907 4175 368
Size: 92 1295 971 432 749 268 1096
Fragments arranged by size:
1295 1096 971 749 432 268 92
AfeI AGC'GCT
Cuts at: 439 439
Size: 4903
AflIII A'CryG_T
Cuts at: 1434 2253 3359 4336 1434
Size: 819 1106 977 2001
Fragments arranged by size:
2001 1106 977 819
AgeI A'CCGG_T
Cuts at: 3291 3291
Size: 4903
AlwNI CAG_nnn'CTG
Cuts at: 1113 2206 3775 1113
Size: 1093 1569 2241
Fragments arranged by size:
2241 1569 1093
ApaLI G'TGCA_C
Cuts at: 451 3673 451
Size: 3222 1681
ApoI r'AATT_y
Cuts at: 832 885 954 983 1728 1795 1905 4256 4512
Size: 53 69 29 745 67 110 2351 256
Cuts at: 4512 832
Size: 1223
Fragments arranged by size:
2351 1223 745 256 110 69 67 53
29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4903
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4903
AvaI C'yCGr_G
Cuts at: 377 420 1921 377
Size: 43 1501 3359
Fragments arranged by size:
3359 1501 43
AvrII C'CTAG_G
Cuts at: 759 1521 759
Size: 762 4141
BamHI G'GATC_C
Cuts at: 1926 1926
Size: 4903
BanI G'GyrC_C
Cuts at: 336 470 914 1917 2331 2579 2614 336
Size: 134 444 1003 414 248 35 2625
Fragments arranged by size:
2625 1003 444 414 248 134 35
BanII G_rGCy'C
Cuts at: 559 1193 1643 1915 2945 559
Size: 634 450 272 1030 2517
Fragments arranged by size:
2517 1030 634 450 272
BbeI G_GCGC'C
Cuts at: 474 2335 2583 474
Size: 1861 248 2794
Fragments arranged by size:
2794 1861 248
BbsI GAAGACnn'nnnn_
Cuts at: 491 788 491
Size: 297 4606
BbvCI CC'TCA_GC
Cuts at: 1234 1337 1234
Size: 103 4800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 194 268 302 1359 1393 160
Size: 34 74 34 1057 34 3670
Fragments arranged by size:
3670 1057 74 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3562 4903 2799
Size: 763 1341 2799
Fragments arranged by size:
2799 1341 763
BclI T'GATC_A
Cuts at: 2421 2421
Size: 4903
BfrBI ATG'CAT
Cuts at: 289 289
Size: 4903
BglII A'GATC_T
Cuts at: 1054 1736 2416 4455 1054
Size: 682 680 2039 1502
Fragments arranged by size:
2039 1502 682 680
BlpI GC'TnA_GC
Cuts at: 2013 2013
Size: 4903
Bme1580I G_kGCm'C
Cuts at: 341 455 2526 2619 3677 341
Size: 114 2071 93 1058 1567
Fragments arranged by size:
2071 1567 1058 114 93
BmgBI CAC'GTC
Cuts at: 1271 1271
Size: 4903
BmrI ACTGGGnnnn_n'
Cuts at: 2113 2194 2529 4675 2113
Size: 81 335 2146 2341
Fragments arranged by size:
2341 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 4903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 951 1144 1495 1512 1587 316
Size: 635 193 351 17 75 3632
Fragments arranged by size:
3632 635 351 193 75 17
Bpu10I CC'TnA_GC
Cuts at: 1234 1337 1234
Size: 103 4800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 1846 2077 2956 3450 3748 1282
Size: 564 231 879 494 298 2437
Fragments arranged by size:
2437 879 564 494 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 674 4607 674
Size: 3933 970
BsaAI yAC'GTr
Cuts at: 1893 2254 1893
Size: 361 4542
BsaBI GATnn'nnATC
Cuts at: 6 1516 2415 4454 6
Size: 1510 899 2039 455
Fragments arranged by size:
2039 1510 899 455
BsaHI Gr'CG_yC
Cuts at: 471 1372 2332 2580 3284 4780 471
Size: 901 960 248 704 1496 594
Fragments arranged by size:
1496 960 901 704 594 248
BsaWI w'CCGG_w
Cuts at: 2090 2611 3291 3565 3712 4123 2090
Size: 521 680 274 147 411 2870
Fragments arranged by size:
2870 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 194 224 289 319 1126 1156 194
Size: 30 65 30 807 30 3941
Fragments arranged by size:
3941 807 65 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 383 1285 1492 1651 383
Size: 902 207 159 3635
Fragments arranged by size:
3635 902 207 159
BseYI C'CCAG_C
Cuts at: 443 941 1011 2326 3084 3663 4159 443
Size: 498 70 1315 758 579 496 1187
Fragments arranged by size:
1315 1187 758 579 498 496 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1268 454
Size: 814 4089
BsiEI CG_ry'CG
Cuts at: 104 2443 2489 3699 104
Size: 2339 46 1210 1308
Fragments arranged by size:
2339 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 455 569 749 1915 2693 2883 3677 455
Size: 114 180 1166 778 190 794 1681
Fragments arranged by size:
1681 1166 794 778 190 180 114
BsmI GAATG_Cn'
Cuts at: 291 1313 291
Size: 1022 3881
Bsp1286I G_dGCh'C
Cuts at: 341 455 559 569 749 1193 1643 1915 2526
Size: 114 104 10 180 444 450 272 611
Cuts at: 2526 2619 2693 2883 2945 3677 341
Size: 93 74 190 62 732 1567
Fragments arranged by size:
1567 732 611 450 444 272 190 180
114 104 93 74 62 10
BspEI T'CCGG_A
Cuts at: 4123 4123
Size: 4903
BspHI T'CATG_A
Cuts at: 4079 4079
Size: 4903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1112 1951 2165 2467 2848 1112
Size: 839 214 302 381 3167
Fragments arranged by size:
3167 839 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3193 3247 4733 4898 50
Size: 3143 54 1486 165 55
Fragments arranged by size:
3143 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 2813 1415
Size: 1398 3505
BsrFI r'CCGG_y
Cuts at: 136 513 2899 3080 3291 136
Size: 377 2386 181 211 1748
Fragments arranged by size:
2386 1748 377 211 181
BssSI C'ACGA_G
Cuts at: 1402 1642 3172 3532 1402
Size: 240 1530 360 2773
Fragments arranged by size:
2773 1530 360 240
BstAPI GCAn_nnn'nTGC
Cuts at: 432 2278 432
Size: 1846 3057
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4903
BstYI r'GATC_y
Cuts at: 1 1054 1598 1736 1926 2393 2416 2751 2997
Size: 1053 544 138 190 467 23 335 246
Cuts at: 2997 4011 4097 4109 4455 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1053 1014 544 467 449 346 335 246
190 138 86 23 12
BtgI C'CryG_G
Cuts at: 815 1185 815
Size: 370 4533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2851 3032 3195 2851
Size: 181 163 4559
Fragments arranged by size:
4559 181 163
BtsI GCAGTG_nn'
Cuts at: 1389 1869 2144 1389
Size: 480 275 4148
Fragments arranged by size:
4148 480 275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 629 664 629
Size: 35 4868
DraI TTT'AAA
Cuts at: 873 1900 873
Size: 1027 3876
DraIII CAC_nnn'GTG
Cuts at: 717 1706 717
Size: 989 3914
DrdI GACnn_nn'nnGTC
Cuts at: 2607 3467 2607
Size: 860 4043
EaeI y'GGCC_r
Cuts at: 354 515 818 1115 2486 2660 3051 3078 4425
Size: 161 303 297 1371 174 391 27 1347
Cuts at: 4425 354
Size: 832
Fragments arranged by size:
1371 1347 832 391 303 297 174 161
27
EagI C'GGCC_G
Cuts at: 2486 2486
Size: 4903
EarI CTCTTCn'nnn_
Cuts at: 2924 3134 2924
Size: 210 4693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1468 3109 3421 3567 4713 1468
Size: 1641 312 146 1146 1658
Fragments arranged by size:
1658 1641 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1913 1913
Size: 4903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 368 460 951 1144 1495 1512 1587 1755
Size: 52 92 491 193 351 17 75 168
Cuts at: 1755 2726 3158 3907 4175 316
Size: 971 432 749 268 1044
Fragments arranged by size:
1044 971 749 491 432 351 268 193
168 92 75 52 17
EcoNI CCTnn'n_nnAGG
Cuts at: 478 1477 478
Size: 999 3904
EcoO109I rG'GnC_Cy
Cuts at: 388 2040 388
Size: 1652 3251
EcoRI G'AATT_C
Cuts at: 1905 1905
Size: 4903
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4903
FspI TGC'GCA
Cuts at: 1381 2682 1381
Size: 1301 3602
HaeII r_GCGC'y
Cuts at: 441 474 2335 2583 3607 4586 441
Size: 33 1861 248 1024 979 758
Fragments arranged by size:
1861 1024 979 758 248 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 226 1431 1463 2743 2773 2775 2805 194
Size: 32 1205 32 1280 30 2 30 2292
Fragments arranged by size:
2292 1280 1205 32 32 30 30 2
HincII GTy'rAC
Cuts at: 187 1821 1940 187
Size: 1634 119 3150
Fragments arranged by size:
3150 1634 119
KasI G'GCGC_C
Cuts at: 470 2331 2579 470
Size: 1861 248 2794
Fragments arranged by size:
2794 1861 248
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4903
MfeI C'AATT_G
Cuts at: 691 691
Size: 4903
MluI A'CGCG_T
Cuts at: 1434 4336 1434
Size: 2902 2001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3574 3758 3574
Size: 184 4719
MscI TGG'CCA
Cuts at: 356 820 2662 356
Size: 464 1842 2597
Fragments arranged by size:
2597 1842 464
MslI CAynn'nnrTG
Cuts at: 1510 1879 2890 3017 1510
Size: 369 1011 127 3396
Fragments arranged by size:
3396 1011 369 127
MspA1I CmG'CkG
Cuts at: 562 731 1110 1815 1833 2012 2326 2686 3701
Size: 169 379 705 18 179 314 360 1015
Cuts at: 3701 3946 562
Size: 245 1519
Fragments arranged by size:
1519 1015 705 379 360 314 245 179
169 18
NaeI GCC'GGC
Cuts at: 138 515 3082 138
Size: 377 2567 1959
Fragments arranged by size:
2567 1959 377
NarI GG'CG_CC
Cuts at: 471 2332 2580 471
Size: 1861 248 2794
Fragments arranged by size:
2794 1861 248
NcoI C'CATG_G
Cuts at: 815 815
Size: 4903
NgoMIV G'CCGG_C
Cuts at: 136 513 3080 136
Size: 377 2567 1959
Fragments arranged by size:
2567 1959 377
NruI TCG'CGA
Cuts at: 3264 3264
Size: 4903
NsiI A_TGCA'T
Cuts at: 291 291
Size: 4903
NspI r_CATG'y
Cuts at: 324 364 1003 1954 2454 3363 324
Size: 40 639 951 500 909 1864
Fragments arranged by size:
1864 951 909 639 500 40
PasI CC'CwG_GG
Cuts at: 550 550
Size: 4903
PciI A'CATG_T
Cuts at: 3359 3359
Size: 4903
PflMI CCAn_nnn'nTGG
Cuts at: 696 792 696
Size: 96 4807
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4903
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 492 524 4042 4074 492
Size: 32 3518 32 1321
Fragments arranged by size:
3518 1321 32 32
PstI C_TGCA'G
Cuts at: 319 350 1109 1948 2633 319
Size: 31 759 839 685 2589
Fragments arranged by size:
2589 839 759 685 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4903
PvuII CAG'CTG
Cuts at: 731 1110 1815 1833 2326 2686 731
Size: 379 705 18 493 360 2948
Fragments arranged by size:
2948 705 493 379 360 18
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4903
SacI G_AGCT'C
Cuts at: 1915 1915
Size: 4903
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4903
SapI GCTCTTCn'nnn_
Cuts at: 2924 3134 2924
Size: 210 4693
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4903
ScaI AGT'ACT
Cuts at: 4420 4420
Size: 4903
SfcI C'TryA_G
Cuts at: 33 315 346 1105 1178 1944 2629 3624 3815
Size: 282 31 759 73 766 685 995 191
Cuts at: 3815 33
Size: 1121
Fragments arranged by size:
1121 995 766 759 685 282 191 73
31
SfoI GGC'GCC
Cuts at: 472 2333 2581 472
Size: 1861 248 2794
Fragments arranged by size:
2794 1861 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4903
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4903
SmlI C'TyrA_G
Cuts at: 1297 1861 2056 2971 3465 3727 1297
Size: 564 195 915 494 262 2473
Fragments arranged by size:
2473 915 564 494 262 195
SnaBI TAC'GTA
Cuts at: 1893 1893
Size: 4903
SpeI A'CTAG_T
Cuts at: 67 738 67
Size: 671 4232
SphI G_CATG'C
Cuts at: 324 364 1954 2454 324
Size: 40 1590 500 2773
Fragments arranged by size:
2773 1590 500 40
StuI AGG'CCT
Cuts at: 1351 1351
Size: 4903
StyI C'CwwG_G
Cuts at: 759 815 1076 1521 2035 2389 759
Size: 56 261 445 514 354 3273
Fragments arranged by size:
3273 514 445 354 261 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 242 386 2484 2617 3076 3287 242
Size: 144 2098 133 459 211 1858
Fragments arranged by size:
2098 1858 459 211 144 133
TatI w'GTAC_w
Cuts at: 305 1166 1558 1695 4418 305
Size: 861 392 137 2723 790
Fragments arranged by size:
2723 861 790 392 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4420 4452 4420
Size: 32 4871
Tth111I GACn'n_nGTC
Cuts at: 2698 2698
Size: 4903
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 925 2396 925
Size: 1471 3432
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4903
XmnI GAAnn'nnTTC
Cuts at: 889 889
Size: 4903
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PasI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
BaeI BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI