Restriction map for
pF1KSDA0210
(Circular) (Six-base) MAPSORT of: pF1KSDA0210.seq Check: 4673 from: 1 to: 5522
pF1KSDA0210 5522 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1549 1549
Size: 5522
AccI GT'mk_AC
Cuts at: 2560 2560
Size: 5522
Acc65I G'GTAC_C
Cuts at: 2538 2538
Size: 5522
AclI AA'CG_TT
Cuts at: 634 5362 634
Size: 4728 794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 1072 1898 2210 3347 3779 4526 4794 290
Size: 782 826 312 1137 432 747 268 1018
Fragments arranged by size:
1137 1018 826 782 747 432 312 268
AflII C'TTAA_G
Cuts at: 1342 1342
Size: 5522
AflIII A'CryG_T
Cuts at: 2874 3978 4955 2874
Size: 1104 977 3441
Fragments arranged by size:
3441 1104 977
AgeI A'CCGG_T
Cuts at: 3910 3910
Size: 5522
AhdI GACnn_n'nnGTC
Cuts at: 195 195
Size: 5522
AleI CACnn'nnGTG
Cuts at: 2491 2491
Size: 5522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1714 1714
Size: 5522
AlwNI CAG_nnn'CTG
Cuts at: 1674 2827 4394 1674
Size: 1153 1567 2802
Fragments arranged by size:
2802 1567 1153
ApaI G_GGCC'C
Cuts at: 2419 2419
Size: 5522
ApaLI G'TGCA_C
Cuts at: 2241 4292 2241
Size: 2051 3471
ApoI r'AATT_y
Cuts at: 580 677 1407 1774 1898 2007 2027 2256 2526
Size: 97 730 367 124 109 20 229 270
Cuts at: 2526 4875 5131 580
Size: 2349 256 971
Fragments arranged by size:
2349 971 730 367 270 256 229 124
109 97 20
AseI AT'TA_AT
Cuts at: 20 356 1511 20
Size: 336 1155 4031
Fragments arranged by size:
4031 1155 336
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
AvaI C'yCGr_G
Cuts at: 504 2542 504
Size: 2038 3484
BamHI G'GATC_C
Cuts at: 2547 2547
Size: 5522
BanI G'GyrC_C
Cuts at: 2538 2952 3200 3235 2538
Size: 414 248 35 4825
Fragments arranged by size:
4825 414 248 35
BanII G_rGCy'C
Cuts at: 2314 2419 2536 3566 2314
Size: 105 117 1030 4270
Fragments arranged by size:
4270 1030 117 105
BbeI G_GCGC'C
Cuts at: 2956 3204 2956
Size: 248 5274
BbvCI CC'TCA_GC
Cuts at: 1204 1623 2424 1204
Size: 419 801 4302
Fragments arranged by size:
4302 801 419
BciVI GTATCCnnnnn_n'
Cuts at: 3420 4181 5522 3420
Size: 761 1341 3420
Fragments arranged by size:
3420 1341 761
BclI T'GATC_A
Cuts at: 536 3042 536
Size: 2506 3016
BfrBI ATG'CAT
Cuts at: 1305 1305
Size: 5522
BglII A'GATC_T
Cuts at: 3037 5074 3037
Size: 2037 3485
BlpI GC'TnA_GC
Cuts at: 2634 2634
Size: 5522
Bme1580I G_kGCm'C
Cuts at: 2245 2419 3147 3240 4296 2245
Size: 174 728 93 1056 3471
Fragments arranged by size:
3471 1056 728 174 93
BmrI ACTGGGnnnn_n'
Cuts at: 818 2371 2734 2815 3150 5294 818
Size: 1553 363 81 335 2144 1046
Fragments arranged by size:
2144 1553 1046 363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1456 3889 1456
Size: 2433 3089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1532 1595 1784 2139 2418 2433 239
Size: 1293 63 189 355 279 15 3328
Fragments arranged by size:
3328 1293 355 279 189 63 15
Bpu10I CC'TnA_GC
Cuts at: 1204 1623 2424 1204
Size: 419 801 4302
Fragments arranged by size:
4302 801 419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 2698 3577 4069 4367 407
Size: 2291 879 492 298 1562
Fragments arranged by size:
2291 1562 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1500 5226 1500
Size: 3726 1796
BsaAI yAC'GTr
Cuts at: 2514 2875 2514
Size: 361 5161
BsaBI GATnn'nnATC
Cuts at: 6 3036 5073 6
Size: 3030 2037 455
Fragments arranged by size:
3030 2037 455
BsaHI Gr'CG_yC
Cuts at: 2953 3201 3903 5399 2953
Size: 248 702 1496 3076
Fragments arranged by size:
3076 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2711 3232 3910 4184 4331 4742 2711
Size: 521 678 274 147 411 3491
Fragments arranged by size:
3491 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1770 1800 2137 2167 2431 2461 1770
Size: 30 337 30 264 30 4831
Fragments arranged by size:
4831 337 264 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 236 1445 1584 1587 236
Size: 1209 139 3 4171
Fragments arranged by size:
4171 1209 139 3
BseYI C'CCAG_C
Cuts at: 1536 2330 2418 2443 2947 3705 4282 4778 1536
Size: 794 88 25 504 758 577 496 2280
Fragments arranged by size:
2280 794 758 577 504 496 88 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1577 800
Size: 777 4745
BsiEI CG_ry'CG
Cuts at: 104 3064 3110 4318 104
Size: 2960 46 1208 1308
Fragments arranged by size:
2960 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1476 2245 2536 3314 3504 4296 1476
Size: 769 291 778 190 792 2702
Fragments arranged by size:
2702 792 778 769 291 190
Bsp1286I G_dGCh'C
Cuts at: 1476 2245 2314 2419 2536 3147 3240 3314 3504
Size: 769 69 105 117 611 93 74 190
Cuts at: 3504 3566 4296 1476
Size: 62 730 2702
Fragments arranged by size:
2702 769 730 611 190 117 105 93
74 69 62
BspEI T'CCGG_A
Cuts at: 4742 4742
Size: 5522
BspHI T'CATG_A
Cuts at: 1442 4698 1442
Size: 3256 2266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 1549 2572 2786 3088 3469 368
Size: 1181 1023 214 302 381 2421
Fragments arranged by size:
2421 1181 1023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3814 3868 5352 5517 50
Size: 3764 54 1484 165 55
Fragments arranged by size:
3764 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1547 2132 3434 1547
Size: 585 1302 3635
Fragments arranged by size:
3635 1302 585
BsrFI r'CCGG_y
Cuts at: 3520 3701 3910 3520
Size: 181 209 5132
Fragments arranged by size:
5132 209 181
BsrGI T'GTAC_A
Cuts at: 1326 1326
Size: 5522
BssSI C'ACGA_G
Cuts at: 1427 3793 4151 1427
Size: 2366 358 2798
Fragments arranged by size:
2798 2366 358
BstAPI GCAn_nnn'nTGC
Cuts at: 392 2899 392
Size: 2507 3015
BstBI TT'CG_AA
Cuts at: 110 3883 110
Size: 3773 1749
BstYI r'GATC_y
Cuts at: 1 1034 1362 2403 2547 3014 3037 3372 3618
Size: 1033 328 1041 144 467 23 335 246
Cuts at: 3618 4630 4716 4728 5074 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1041 1033 1012 467 449 346 335 328
246 144 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3472 3653 3816 3472
Size: 181 163 5178
Fragments arranged by size:
5178 181 163
BtsI GCAGTG_nn'
Cuts at: 887 1560 2307 2765 887
Size: 673 747 458 3644
Fragments arranged by size:
3644 747 673 458
DraI TTT'AAA
Cuts at: 2521 2521
Size: 5522
DrdI GACnn_nn'nnGTC
Cuts at: 3228 4086 3228
Size: 858 4664
EaeI y'GGCC_r
Cuts at: 519 2160 2322 3107 3281 3672 3699 5044 519
Size: 1641 162 785 174 391 27 1345 997
Fragments arranged by size:
1641 1345 997 785 391 174 162 27
EagI C'GGCC_G
Cuts at: 3107 3107
Size: 5522
EarI CTCTTCn'nnn_
Cuts at: 1764 2108 2229 2342 3545 3755 1764
Size: 344 121 113 1203 210 3531
Fragments arranged by size:
3531 1203 344 210 121 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3730 4040 4186 5332 3730
Size: 310 146 1146 3920
Fragments arranged by size:
3920 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2534 2534
Size: 5522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 290 1072 1532 1595 1784 1898 2139 2210
Size: 51 782 460 63 189 114 241 71
Cuts at: 2210 2418 2433 3347 3779 4526 4794 239
Size: 208 15 914 432 747 268 967
Fragments arranged by size:
967 914 782 747 460 432 268 241
208 189 114 71 63 51 15
EcoO109I rG'GnC_Cy
Cuts at: 1164 1619 1632 2373 2661 1164
Size: 455 13 741 288 4025
Fragments arranged by size:
4025 741 455 288 13
EcoRI G'AATT_C
Cuts at: 1774 2256 2526 1774
Size: 482 270 4770
Fragments arranged by size:
4770 482 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 275 586 3541 275
Size: 311 2955 2256
Fragments arranged by size:
2955 2256 311
FspI TGC'GCA
Cuts at: 3303 3303
Size: 5522
HaeII r_GCGC'y
Cuts at: 2956 3204 4226 5205 2956
Size: 248 1022 979 3273
Fragments arranged by size:
3273 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 528 560 1026 1058 1315 1347 1953
Size: 32 315 32 466 32 257 32 606
Cuts at: 1953 1985 3364 3394 3396 3426 181
Size: 32 1379 30 2 30 2277
Fragments arranged by size:
2277 1379 606 466 315 257 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1029 2561 1029
Size: 1532 3990
HindIII A'AGCT_T
Cuts at: 791 791
Size: 5522
KasI G'GCGC_C
Cuts at: 2952 3200 2952
Size: 248 5274
KpnI G_GTAC'C
Cuts at: 2542 2542
Size: 5522
MfeI C'AATT_G
Cuts at: 1007 1702 1007
Size: 695 4827
MluI A'CGCG_T
Cuts at: 4955 4955
Size: 5522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4193 4377 4193
Size: 184 5338
MscI TGG'CCA
Cuts at: 521 3283 521
Size: 2762 2760
MslI CAynn'nnrTG
Cuts at: 806 1696 2491 3511 3638 806
Size: 890 795 1020 127 2690
Fragments arranged by size:
2690 1020 890 795 127
MspA1I CmG'CkG
Cuts at: 366 2159 2633 2947 3307 4320 4565 366
Size: 1793 474 314 360 1013 245 1323
Fragments arranged by size:
1793 1323 1013 474 360 314 245
NaeI GCC'GGC
Cuts at: 3703 3703
Size: 5522
NarI GG'CG_CC
Cuts at: 2953 3201 2953
Size: 248 5274
NgoMIV G'CCGG_C
Cuts at: 3701 3701
Size: 5522
NsiI A_TGCA'T
Cuts at: 1307 1307
Size: 5522
NspI r_CATG'y
Cuts at: 744 805 1080 1695 2575 3075 3982 744
Size: 61 275 615 880 500 907 2284
Fragments arranged by size:
2284 907 880 615 500 275 61
PciI A'CATG_T
Cuts at: 3978 3978
Size: 5522
PfoI T'CCnGG_A
Cuts at: 1037 1037
Size: 5522
PmeI GTTT'AAAC
Cuts at: 2521 2521
Size: 5522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 4661
Size: 32 5490
PpuMI rG'GwC_Cy
Cuts at: 1164 1619 1164
Size: 455 5067
PshAI GACnn'nnGTC
Cuts at: 431 431
Size: 5522
PsiI TTA'TAA
Cuts at: 731 731
Size: 5522
PspOMI G'GGCC_C
Cuts at: 2415 2415
Size: 5522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 240 272 240
Size: 32 5490
PstI C_TGCA'G
Cuts at: 164 1163 2569 3254 164
Size: 999 1406 685 2432
Fragments arranged by size:
2432 1406 999 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5522
PvuII CAG'CTG
Cuts at: 366 2947 3307 366
Size: 2581 360 2581
Fragments arranged by size:
2581 2581 360
RsrII CG'GwC_CG
Cuts at: 3717 3717
Size: 5522
SacI G_AGCT'C
Cuts at: 2536 2536
Size: 5522
SalI G'TCGA_C
Cuts at: 2559 2559
Size: 5522
SapI GCTCTTCn'nnn_
Cuts at: 3545 3755 3545
Size: 210 5312
SbfI CC_TGCA'GG
Cuts at: 1163 2569 1163
Size: 1406 4116
ScaI AGT'ACT
Cuts at: 5039 5039
Size: 5522
SfcI C'TryA_G
Cuts at: 33 160 288 761 1159 2565 3250 4243 4434
Size: 127 128 473 398 1406 685 993 191
Cuts at: 4434 33
Size: 1121
Fragments arranged by size:
1406 1121 993 685 473 398 191 128
127
SfoI GGC'GCC
Cuts at: 2954 3202 2954
Size: 248 5274
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5522
SmaI CCC'GGG
Cuts at: 2544 2544
Size: 5522
SmlI C'TyrA_G
Cuts at: 422 1342 2677 3592 4084 4346 422
Size: 920 1335 915 492 262 1598
Fragments arranged by size:
1598 1335 920 915 492 262
SnaBI TAC'GTA
Cuts at: 2514 2514
Size: 5522
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5522
SphI G_CATG'C
Cuts at: 744 1695 2575 3075 744
Size: 951 880 500 3191
Fragments arranged by size:
3191 951 880 500
SspI AAT'ATT
Cuts at: 1983 1983
Size: 5522
StuI AGG'CCT
Cuts at: 2221 2221
Size: 5522
StyI C'CwwG_G
Cuts at: 913 2181 2656 3010 913
Size: 1268 475 354 3425
Fragments arranged by size:
3425 1268 475 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1116 3105 3238 3697 3906 1116
Size: 1989 133 459 209 2732
Fragments arranged by size:
2732 1989 459 209 133
TatI w'GTAC_w
Cuts at: 1326 5037 1326
Size: 3711 1811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1770 1802 1924 1956 2137 2169 5039 5071 1770
Size: 32 122 32 181 32 2870 32 2221
Fragments arranged by size:
2870 2221 181 122 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3319 3319
Size: 5522
XbaI T'CTAG_A
Cuts at: 2553 2553
Size: 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3017 3017
Size: 5522
XmaI C'CCGG_G
Cuts at: 2542 2542
Size: 5522
XmnI GAAnn'nnTTC
Cuts at: 252 580 252
Size: 328 5194
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI AscI AvrII BaeI BbsI BcgI
BglI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI ZraI