Restriction map for
pF1KSDA0211
(Circular) (Six-base) MAPSORT of: pF1KSDA0211.seq Check: 9190 from: 1 to: 6938
pF1KSDA0211 6938 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2778 3265 2778
Size: 487 6451
AatII G_ACGT'C
Cuts at: 3364 3364
Size: 6938
AccI GT'mk_AC
Cuts at: 3976 3976
Size: 6938
Acc65I G'GTAC_C
Cuts at: 913 3161 3954 913
Size: 2248 793 3897
Fragments arranged by size:
3897 2248 793
AclI AA'CG_TT
Cuts at: 6778 6778
Size: 6938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1679 2897 3044 4763 5195 5942 6210 1679
Size: 1218 147 1719 432 747 268 2407
Fragments arranged by size:
2407 1719 1218 747 432 268 147
AflIII A'CryG_T
Cuts at: 2853 3119 4290 5394 6371 2853
Size: 266 1171 1104 977 3420
Fragments arranged by size:
3420 1171 1104 977 266
AgeI A'CCGG_T
Cuts at: 5326 5326
Size: 6938
AhdI GACnn_n'nnGTC
Cuts at: 2895 2895
Size: 6938
AleI CACnn'nnGTG
Cuts at: 443 2033 2537 2609 2974 443
Size: 1590 504 72 365 4407
Fragments arranged by size:
4407 1590 504 365 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1603 3013 1603
Size: 1410 5528
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2092 2124 3173 3205 2092
Size: 32 1049 32 5825
Fragments arranged by size:
5825 1049 32 32
AlwNI CAG_nnn'CTG
Cuts at: 571 1318 2079 2233 2367 2390 4243 5810 571
Size: 747 761 154 134 23 1853 1567 1699
Fragments arranged by size:
1853 1699 1567 761 747 154 134 23
ApaI G_GGCC'C
Cuts at: 1067 1526 3838 1067
Size: 459 2312 4167
Fragments arranged by size:
4167 2312 459
ApaLI G'TGCA_C
Cuts at: 1633 1974 5708 1633
Size: 341 3734 2863
Fragments arranged by size:
3734 2863 341
ApoI r'AATT_y
Cuts at: 451 1437 3942 6291 6547 451
Size: 986 2505 2349 256 842
Fragments arranged by size:
2505 2349 986 842 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6938
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6938
AvaI C'yCGr_G
Cuts at: 902 1526 2945 3528 3535 3958 902
Size: 624 1419 583 7 423 3882
Fragments arranged by size:
3882 1419 624 583 423 7
AvrII C'CTAG_G
Cuts at: 934 934
Size: 6938
BamHI G'GATC_C
Cuts at: 576 816 1304 1862 3963 576
Size: 240 488 558 2101 3551
Fragments arranged by size:
3551 2101 558 488 240
BanI G'GyrC_C
Cuts at: 913 1401 1737 2136 3161 3445 3750 3954 4368
Size: 488 336 399 1025 284 305 204 414
Cuts at: 4368 4616 4651 913
Size: 248 35 3200
Fragments arranged by size:
3200 1025 488 414 399 336 305 284
248 204 35
BanII G_rGCy'C
Cuts at: 418 1067 1129 1450 1526 2057 3536 3739 3838
Size: 649 62 321 76 531 1479 203 99
Cuts at: 3838 3952 4982 418
Size: 114 1030 2374
Fragments arranged by size:
2374 1479 1030 649 531 321 203 114
99 76 62
BbeI G_GCGC'C
Cuts at: 4372 4620 4372
Size: 248 6690
BbsI GAAGACnn'nnnn_
Cuts at: 1685 1779 2724 2790 2903 1685
Size: 94 945 66 113 5720
Fragments arranged by size:
5720 945 113 94 66
BbvCI CC'TCA_GC
Cuts at: 3058 3557 3628 3058
Size: 499 71 6368
Fragments arranged by size:
6368 499 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2037 2071 3810 3844 2037
Size: 34 1739 34 5131
Fragments arranged by size:
5131 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4836 5597 6938 4836
Size: 761 1341 4836
Fragments arranged by size:
4836 1341 761
BclI T'GATC_A
Cuts at: 717 3867 4458 717
Size: 3150 591 3197
Fragments arranged by size:
3197 3150 591
BfrBI ATG'CAT
Cuts at: 3330 3330
Size: 6938
BglI GCCn_nnn'nGGC
Cuts at: 208 991 1000 2939 208
Size: 783 9 1939 4207
Fragments arranged by size:
4207 1939 783 9
BglII A'GATC_T
Cuts at: 420 4453 6490 420
Size: 4033 2037 868
Fragments arranged by size:
4033 2037 868
BlpI GC'TnA_GC
Cuts at: 4050 4050
Size: 6938
Bme1580I G_kGCm'C
Cuts at: 1067 1526 1637 1742 1823 1978 2397 2809 2818
Size: 459 111 105 81 155 419 412 9
Cuts at: 2818 3755 3838 4563 4656 5712 1067
Size: 937 83 725 93 1056 2293
Fragments arranged by size:
2293 1056 937 725 459 419 412 155
111 105 93 83 81 9
BmgBI CAC'GTC
Cuts at: 2856 2856
Size: 6938
BmrI ACTGGGnnnn_n'
Cuts at: 218 383 661 4150 4231 4566 6710 218
Size: 165 278 3489 81 335 2144 446
Fragments arranged by size:
3489 2144 446 335 278 165 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3753 5305 3753
Size: 1552 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 413 526 543 694 1153 1915 2192 2890
Size: 174 113 17 151 459 762 277 698
Cuts at: 2890 3328 3425 239
Size: 438 97 3752
Fragments arranged by size:
3752 762 698 459 438 277 174 151
113 97 17
Bpu10I CC'TnA_GC
Cuts at: 1930 3058 3557 3628 1930
Size: 1128 499 71 5240
Fragments arranged by size:
5240 1128 499 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2913 4114 4993 5485 5783 2913
Size: 1201 879 492 298 4068
Fragments arranged by size:
4068 1201 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3656 6642 3656
Size: 2986 3952
BsaAI yAC'GTr
Cuts at: 1869 3120 3930 4291 1869
Size: 1251 810 361 4516
Fragments arranged by size:
4516 1251 810 361
BsaBI GATnn'nnATC
Cuts at: 6 4452 6489 6
Size: 4446 2037 455
Fragments arranged by size:
4446 2037 455
BsaHI Gr'CG_yC
Cuts at: 2795 3361 4369 4617 5319 6815 2795
Size: 566 1008 248 702 1496 2918
Fragments arranged by size:
2918 1496 1008 702 566 248
BsaWI w'CCGG_w
Cuts at: 693 819 2246 4127 4648 5326 5600 5747 6158
Size: 126 1427 1881 521 678 274 147 411
Cuts at: 6158 693
Size: 1473
Fragments arranged by size:
1881 1473 1427 678 521 411 274 147
126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 653 683 2680 2710 653
Size: 30 1997 30 4881
Fragments arranged by size:
4881 1997 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 2195 2344 3583 3685 3688 3691 3700 3745
Size: 1951 149 1239 102 3 3 9 45
Cuts at: 3745 3760 3837 244
Size: 15 77 3345
Fragments arranged by size:
3345 1951 1239 149 102 77 45 15
9 3 3
BseYI C'CCAG_C
Cuts at: 1378 1507 2072 2148 2669 2681 2699 3011 3283
Size: 129 565 76 521 12 18 312 272
Cuts at: 3283 3831 4363 5121 5698 6194 1378
Size: 548 532 758 577 496 2122
Fragments arranged by size:
2122 758 577 565 548 532 521 496
312 272 129 76 18 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2034 2291 2403 2482 2851 3252 2034
Size: 257 112 79 369 401 5720
Fragments arranged by size:
5720 401 369 257 112 79
BsiEI CG_ry'CG
Cuts at: 104 320 3147 4480 4526 5734 104
Size: 216 2827 1333 46 1208 1308
Fragments arranged by size:
2827 1333 1308 1208 216 46
BsiHKAI G_wGCw'C
Cuts at: 1418 1582 1637 1978 1987 2719 2866 3952 4730
Size: 164 55 341 9 732 147 1086 778
Cuts at: 4730 4920 5712 1418
Size: 190 792 2644
Fragments arranged by size:
2644 1086 792 778 732 341 190 164
147 55 9
BsmI GAATG_Cn'
Cuts at: 2614 2614
Size: 6938
BsmBI CGTCTCn'nnnn_
Cuts at: 1898 1898
Size: 6938
Bsp1286I G_dGCh'C
Cuts at: 418 1067 1129 1418 1450 1526 1582 1637 1742
Size: 649 62 289 32 76 56 55 105
Cuts at: 1742 1823 1978 1987 2057 2397 2719 2809 2818
Size: 81 155 9 70 340 322 90 9
Cuts at: 2818 2866 3536 3739 3755 3838 3952 4563 4656
Size: 48 670 203 16 83 114 611 93
Cuts at: 4656 4730 4920 4982 5712 418
Size: 74 190 62 730 1644
Fragments arranged by size:
1644 730 670 649 611 340 322 289
203 190 155 114 105 93 90 83
81 76 74 70 62 62 56 55
48 32 16 9 9
BspEI T'CCGG_A
Cuts at: 819 6158 819
Size: 5339 1599
BspHI T'CATG_A
Cuts at: 6114 6114
Size: 6938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 2356 2778 3085 3265 3988 4202 4504 4885
Size: 1431 422 307 180 723 214 302 381
Cuts at: 4885 925
Size: 2978
Fragments arranged by size:
2978 1431 723 422 381 307 302 214
180
BsrBI CCG'CTC
Cuts at: 50 2459 5230 5284 6768 6933 50
Size: 2409 2771 54 1484 165 55
Fragments arranged by size:
2771 2409 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1364 3459 4850 1364
Size: 2095 1391 3452
Fragments arranged by size:
3452 2095 1391
BsrFI r'CCGG_y
Cuts at: 315 1872 1946 3170 3790 4936 5117 5326 315
Size: 1557 74 1224 620 1146 181 209 1927
Fragments arranged by size:
1927 1557 1224 1146 620 209 181 74
BssSI C'ACGA_G
Cuts at: 778 5209 5567 778
Size: 4431 358 2149
Fragments arranged by size:
4431 2149 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1982 4315 1982
Size: 2333 4605
BstBI TT'CG_AA
Cuts at: 110 5299 110
Size: 5189 1749
BstEII G'GTnAC_C
Cuts at: 1346 1346
Size: 6938
BstXI CCAn_nnnn'nTGG
Cuts at: 445 1011 1942 2976 445
Size: 566 931 1034 4407
Fragments arranged by size:
4407 1034 931 566
BstYI r'GATC_y
Cuts at: 1 420 432 576 816 1053 1304 1314 1862
Size: 419 12 144 240 237 251 10 548
Cuts at: 1862 3963 4430 4453 4788 5034 6046 6132 6144
Size: 2101 467 23 335 246 1012 86 12
Cuts at: 6144 6490 1
Size: 346 449
Fragments arranged by size:
2101 1012 548 467 449 419 346 335
251 246 240 237 144 86 23 12
12 10
Bsu36I CC'TnA_GG
Cuts at: 572 1424 3395 3845 3920 572
Size: 852 1971 450 75 3590
Fragments arranged by size:
3590 1971 852 450 75
BtgI C'CryG_G
Cuts at: 125 2132 2624 2868 3052 3141 3755 125
Size: 2007 492 244 184 89 614 3308
Fragments arranged by size:
3308 2007 614 492 244 184 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4888 5069 5232 4888
Size: 181 163 6594
Fragments arranged by size:
6594 181 163
BtsI GCAGTG_nn'
Cuts at: 1191 1585 2071 2196 2589 2648 3181 3716 4181
Size: 394 486 125 393 59 533 535 465
Cuts at: 4181 1191
Size: 3948
Fragments arranged by size:
3948 535 533 486 465 394 393 125
59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 177 212 177
Size: 35 6903
DraI TTT'AAA
Cuts at: 3937 3937
Size: 6938
DraIII CAC_nnn'GTG
Cuts at: 226 1755 1982 3567 3657 226
Size: 1529 227 1585 90 3507
Fragments arranged by size:
3507 1585 1529 227 90
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2296 4644 5502 1689
Size: 607 2348 858 3125
Fragments arranged by size:
3125 2348 858 607
EaeI y'GGCC_r
Cuts at: 317 1041 1640 1874 1944 3006 3858 4523 4697
Size: 724 599 234 70 1062 852 665 174
Cuts at: 4697 5088 5115 6460 317
Size: 391 27 1345 795
Fragments arranged by size:
1345 1062 852 795 724 665 599 391
234 174 70 27
EagI C'GGCC_G
Cuts at: 317 4523 317
Size: 4206 2732
EarI CTCTTCn'nnn_
Cuts at: 1406 1918 2005 2737 3474 3646 3677 4961 5171
Size: 512 87 732 737 172 31 1284 210
Cuts at: 5171 1406
Size: 3173
Fragments arranged by size:
3173 1284 737 732 512 210 172 87
31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1965 5146 5456 5602 6748 1965
Size: 3181 310 146 1146 2155
Fragments arranged by size:
3181 2155 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3950 3950
Size: 6938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 413 526 543 694 1153 1679 1915 2192
Size: 174 113 17 151 459 526 236 277
Cuts at: 2192 2890 2897 3044 3328 3425 4763 5195 5942
Size: 698 7 147 284 97 1338 432 747
Cuts at: 5942 6210 239
Size: 268 967
Fragments arranged by size:
1338 967 747 698 526 459 432 284
277 268 236 174 151 147 113 97
17 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1427 1427
Size: 6938
EcoO109I rG'GnC_Cy
Cuts at: 1063 1374 1523 1530 1797 2448 4077 1063
Size: 311 149 7 267 651 1629 3924
Fragments arranged by size:
3924 1629 651 311 267 149 7
EcoRI G'AATT_C
Cuts at: 3942 3942
Size: 6938
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4957 4957
Size: 6938
FspI TGC'GCA
Cuts at: 3211 4719 3211
Size: 1508 5430
HaeII r_GCGC'y
Cuts at: 1364 4372 4620 5642 6621 1364
Size: 3008 248 1022 979 1681
Fragments arranged by size:
3008 1681 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 1486 1518 2881 2911 2913 2943 3129
Size: 32 801 32 1363 30 2 30 186
Cuts at: 3129 3161 3787 3819 4780 4810 4812 4842 653
Size: 32 626 32 961 30 2 30 2749
Fragments arranged by size:
2749 1363 961 801 626 186 32 32
32 32 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 3877 3977 3877
Size: 100 6838
HindIII A'AGCT_T
Cuts at: 845 845
Size: 6938
KasI G'GCGC_C
Cuts at: 4368 4616 4368
Size: 248 6690
KpnI G_GTAC'C
Cuts at: 917 3165 3958 917
Size: 2248 793 3897
Fragments arranged by size:
3897 2248 793
MluI A'CGCG_T
Cuts at: 6371 6371
Size: 6938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1835 3127 5609 5793 1835
Size: 1292 2482 184 2980
Fragments arranged by size:
2980 2482 1292 184
MscI TGG'CCA
Cuts at: 1043 1642 3860 4699 1043
Size: 599 2218 839 3282
Fragments arranged by size:
3282 2218 839 599
MslI CAynn'nnrTG
Cuts at: 265 443 2033 2537 2609 2974 4927 5054 265
Size: 178 1590 504 72 365 1953 127 2149
Fragments arranged by size:
2149 1953 1590 504 365 178 127 72
MspA1I CmG'CkG
Cuts at: 1511 2076 2403 2917 3011 3054 3700 3808 4049
Size: 565 327 514 94 43 646 108 241
Cuts at: 4049 4363 4723 5736 5981 1511
Size: 314 360 1013 245 2468
Fragments arranged by size:
2468 1013 646 565 514 360 327 314
245 241 108 94 43
NaeI GCC'GGC
Cuts at: 317 1874 1948 3172 5119 317
Size: 1557 74 1224 1947 2136
Fragments arranged by size:
2136 1947 1557 1224 74
NarI GG'CG_CC
Cuts at: 4369 4617 4369
Size: 248 6690
NcoI C'CATG_G
Cuts at: 125 2624 3755 125
Size: 2499 1131 3308
Fragments arranged by size:
3308 2499 1131
NdeI CA'TA_TG
Cuts at: 262 710 262
Size: 448 6490
NgoMIV G'CCGG_C
Cuts at: 315 1872 1946 3170 5117 315
Size: 1557 74 1224 1947 2136
Fragments arranged by size:
2136 1947 1557 1224 74
NsiI A_TGCA'T
Cuts at: 3332 3332
Size: 6938
NspI r_CATG'y
Cuts at: 3991 4491 5398 3991
Size: 500 907 5531
Fragments arranged by size:
5531 907 500
PasI CC'CwG_GG
Cuts at: 1452 1452
Size: 6938
PciI A'CATG_T
Cuts at: 5394 5394
Size: 6938
PflMI CCAn_nnn'nTGG
Cuts at: 2079 3424 2079
Size: 1345 5593
PfoI T'CCnGG_A
Cuts at: 671 2579 671
Size: 1908 5030
PmeI GTTT'AAAC
Cuts at: 3937 3937
Size: 6938
PmlI CAC'GTG
Cuts at: 1869 1869
Size: 6938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6077 6109 6077
Size: 32 6906
PpuMI rG'GwC_Cy
Cuts at: 1530 1797 2448 1530
Size: 267 651 6020
Fragments arranged by size:
6020 651 267
PsiI TTA'TAA
Cuts at: 2711 2711
Size: 6938
PspOMI G'GGCC_C
Cuts at: 1063 1522 3834 1063
Size: 459 2312 4167
Fragments arranged by size:
4167 2312 459
PspXI vC'TCGA_Gb
Cuts at: 3535 3535
Size: 6938
PstI C_TGCA'G
Cuts at: 1186 1516 1592 2655 3985 4670 1186
Size: 330 76 1063 1330 685 3454
Fragments arranged by size:
3454 1330 1063 685 330 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6938
PvuII CAG'CTG
Cuts at: 1511 2076 3808 4363 4723 1511
Size: 565 1732 555 360 3726
Fragments arranged by size:
3726 1732 565 555 360
RsrII CG'GwC_CG
Cuts at: 5133 5133
Size: 6938
SacI G_AGCT'C
Cuts at: 3952 3952
Size: 6938
SacII CC_GC'GG
Cuts at: 3055 3055
Size: 6938
SalI G'TCGA_C
Cuts at: 3975 3975
Size: 6938
SanDI GG'GwC_CC
Cuts at: 1530 1530
Size: 6938
SapI GCTCTTCn'nnn_
Cuts at: 1406 1918 2005 4961 5171 1406
Size: 512 87 2956 210 3173
Fragments arranged by size:
3173 2956 512 210 87
SbfI CC_TGCA'GG
Cuts at: 3985 3985
Size: 6938
ScaI AGT'ACT
Cuts at: 6455 6455
Size: 6938
SexAI A'CCwGG_T
Cuts at: 1751 3563 1751
Size: 1812 5126
SfcI C'TryA_G
Cuts at: 33 1182 1512 1588 2520 2651 3981 4666 5659
Size: 1149 330 76 932 131 1330 685 993
Cuts at: 5659 5850 33
Size: 191 1121
Fragments arranged by size:
1330 1149 1121 993 932 685 330 191
131 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 1000 1000
Size: 6938
SfoI GGC'GCC
Cuts at: 4370 4618 4370
Size: 248 6690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6938
SmaI CCC'GGG
Cuts at: 904 1528 3960 904
Size: 624 2432 3882
Fragments arranged by size:
3882 2432 624
SmlI C'TyrA_G
Cuts at: 2928 2945 3535 4093 5008 5500 5762 2928
Size: 17 590 558 915 492 262 4104
Fragments arranged by size:
4104 915 590 558 492 262 17
SnaBI TAC'GTA
Cuts at: 3930 3930
Size: 6938
SpeI A'CTAG_T
Cuts at: 67 2971 67
Size: 2904 4034
SphI G_CATG'C
Cuts at: 3991 4491 3991
Size: 500 6438
StuI AGG'CCT
Cuts at: 3021 3886 3021
Size: 865 6073
StyI C'CwwG_G
Cuts at: 125 203 758 890 934 986 2555 2624 3755
Size: 78 555 132 44 52 1569 69 1131
Cuts at: 3755 4072 4426 125
Size: 317 354 2637
Fragments arranged by size:
2637 1569 1131 555 354 317 132 78
69 52 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 237 635 2683 2695 2881 4521 4654 5113 5322
Size: 398 2048 12 186 1640 133 459 209
Cuts at: 5322 237
Size: 1853
Fragments arranged by size:
2048 1853 1640 459 398 209 186 133
12
TatI w'GTAC_w
Cuts at: 6453 6453
Size: 6938
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2680 2712 3898 3930 6455 6487 2680
Size: 32 1186 32 2525 32 3131
Fragments arranged by size:
3131 2525 1186 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4735 4735
Size: 6938
XbaI T'CTAG_A
Cuts at: 3969 3969
Size: 6938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4433 4433
Size: 6938
XhoI C'TCGA_G
Cuts at: 2945 3535 2945
Size: 590 6348
XmaI C'CCGG_G
Cuts at: 902 1526 3958 902
Size: 624 2432 3882
Fragments arranged by size:
3882 2432 624
ZraI GAC'GTC
Cuts at: 3362 3362
Size: 6938
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BmtI BsiWI BsrGI BssHII
BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PshAI PsrI SgrAI SrfI SspI
SwaI XmnI