Restriction map for
pF1KSDA0212
(Circular) (Six-base) MAPSORT of: pF1KSDA0212.seq Check: 5603 from: 1 to: 5108
pF1KSDA0212 5108 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1452 1452
Size: 5108
AccI GT'mk_AC
Cuts at: 1077 2146 1077
Size: 1069 4039
Acc65I G'GTAC_C
Cuts at: 2124 2124
Size: 5108
AclI AA'CG_TT
Cuts at: 4948 4948
Size: 5108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 2933 3365 4112 4380 1946
Size: 987 432 747 268 2674
Fragments arranged by size:
2674 987 747 432 268
AfeI AGC'GCT
Cuts at: 140 232 475 140
Size: 92 243 4773
Fragments arranged by size:
4773 243 92
AflII C'TTAA_G
Cuts at: 1332 1332
Size: 5108
AflIII A'CryG_T
Cuts at: 1397 1850 2460 3564 4541 1397
Size: 453 610 1104 977 1964
Fragments arranged by size:
1964 1104 977 610 453
AgeI A'CCGG_T
Cuts at: 3496 3496
Size: 5108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 521 2080 521
Size: 1559 3549
AlwNI CAG_nnn'CTG
Cuts at: 261 1467 1686 2413 3980 261
Size: 1206 219 727 1567 1389
Fragments arranged by size:
1567 1389 1206 727 219
ApaI G_GGCC'C
Cuts at: 212 302 620 212
Size: 90 318 4700
Fragments arranged by size:
4700 318 90
ApaLI G'TGCA_C
Cuts at: 1960 3878 1960
Size: 1918 3190
ApoI r'AATT_y
Cuts at: 717 1060 1915 2112 4461 4717 717
Size: 343 855 197 2349 256 1108
Fragments arranged by size:
2349 1108 855 343 256 197
AseI AT'TA_AT
Cuts at: 20 20
Size: 5108
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
AvaI C'yCGr_G
Cuts at: 294 980 2128 294
Size: 686 1148 3274
Fragments arranged by size:
3274 1148 686
BamHI G'GATC_C
Cuts at: 2133 2133
Size: 5108
BanI G'GyrC_C
Cuts at: 484 1222 1534 2124 2538 2786 2821 484
Size: 738 312 590 414 248 35 2771
Fragments arranged by size:
2771 738 590 414 312 248 35
BanII G_rGCy'C
Cuts at: 212 271 302 373 590 620 2122 3152 212
Size: 59 31 71 217 30 1502 1030 2168
Fragments arranged by size:
2168 1502 1030 217 71 59 31 30
BbeI G_GCGC'C
Cuts at: 488 2542 2790 488
Size: 2054 248 2806
Fragments arranged by size:
2806 2054 248
BbsI GAAGACnn'nnnn_
Cuts at: 189 1292 1760 1823 189
Size: 1103 468 63 3474
Fragments arranged by size:
3474 1103 468 63
BciVI GTATCCnnnnn_n'
Cuts at: 333 690 3006 3767 5108 333
Size: 357 2316 761 1341 333
Fragments arranged by size:
2316 1341 761 357 333
BclI T'GATC_A
Cuts at: 752 2628 752
Size: 1876 3232
BfrBI ATG'CAT
Cuts at: 691 691
Size: 5108
BglI GCCn_nnn'nGGC
Cuts at: 275 336 275
Size: 61 5047
BglII A'GATC_T
Cuts at: 2623 4660 2623
Size: 2037 3071
BlpI GC'TnA_GC
Cuts at: 241 2220 241
Size: 1979 3129
Bme1580I G_kGCm'C
Cuts at: 212 302 510 620 1539 1964 2733 2826 3882
Size: 90 208 110 919 425 769 93 1056
Cuts at: 3882 212
Size: 1438
Fragments arranged by size:
1438 1056 919 769 425 208 110 93
90
BmgBI CAC'GTC
Cuts at: 1734 1734
Size: 5108
BmrI ACTGGGnnnn_n'
Cuts at: 432 1206 2320 2401 2736 4880 432
Size: 774 1114 81 335 2144 660
Fragments arranged by size:
2144 1114 774 660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 5108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1983 1983
Size: 5108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2284 3163 3655 3953 2284
Size: 879 492 298 3439
Fragments arranged by size:
3439 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1364 1590 1777 4812 1364
Size: 226 187 3035 1660
Fragments arranged by size:
3035 1660 226 187
BsaAI yAC'GTr
Cuts at: 432 2100 2461 432
Size: 1668 361 3079
Fragments arranged by size:
3079 1668 361
BsaBI GATnn'nnATC
Cuts at: 6 1889 2622 4659 6
Size: 1883 733 2037 455
Fragments arranged by size:
2037 1883 733 455
BsaHI Gr'CG_yC
Cuts at: 390 485 2539 2787 3489 4985 390
Size: 95 2054 248 702 1496 513
Fragments arranged by size:
2054 1496 702 513 248 95
BsaWI w'CCGG_w
Cuts at: 1142 1757 2297 2818 3496 3770 3917 4328 1142
Size: 615 540 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 615 540 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1987 2017 1987
Size: 30 5078
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 279 928 1615 2046 151
Size: 128 649 687 431 3213
Fragments arranged by size:
3213 687 649 431 128
BseYI C'CCAG_C
Cuts at: 204 211 414 435 2533 3291 3868 4364 204
Size: 7 203 21 2098 758 577 496 948
Fragments arranged by size:
2098 948 758 577 496 203 21 7
BsiEI CG_ry'CG
Cuts at: 104 444 612 624 2650 2696 3904 104
Size: 340 168 12 2026 46 1208 1308
Fragments arranged by size:
2026 1308 1208 340 168 46 12
BsiHKAI G_wGCw'C
Cuts at: 161 590 1964 2122 2900 3090 3882 161
Size: 429 1374 158 778 190 792 1387
Fragments arranged by size:
1387 1374 792 778 429 190 158
Bsp1286I G_dGCh'C
Cuts at: 161 212 271 302 373 510 590 620 1539
Size: 51 59 31 71 137 80 30 919
Cuts at: 1539 1964 2122 2733 2826 2900 3090 3152 3882
Size: 425 158 611 93 74 190 62 730
Cuts at: 3882 161
Size: 1387
Fragments arranged by size:
1387 919 730 611 425 190 158 137
93 80 74 71 62 59 51 31
30
BspEI T'CCGG_A
Cuts at: 1142 4328 1142
Size: 3186 1922
BspHI T'CATG_A
Cuts at: 1977 4284 1977
Size: 2307 2801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1452 2158 2372 2674 3055 1452
Size: 706 214 302 381 3505
Fragments arranged by size:
3505 706 381 302 214
BsrBI CCG'CTC
Cuts at: 50 240 3400 3454 4938 5103 50
Size: 190 3160 54 1484 165 55
Fragments arranged by size:
3160 1484 190 165 55 54
BsrDI GCAATG_nn'
Cuts at: 136 1155 1176 1362 3020 136
Size: 1019 21 186 1658 2224
Fragments arranged by size:
2224 1658 1019 186 21
BsrFI r'CCGG_y
Cuts at: 308 1042 3106 3287 3496 308
Size: 734 2064 181 209 1920
Fragments arranged by size:
2064 1920 734 209 181
BssSI C'ACGA_G
Cuts at: 3379 3737 3379
Size: 358 4750
BstAPI GCAn_nnn'nTGC
Cuts at: 2485 2485
Size: 5108
BstBI TT'CG_AA
Cuts at: 110 3469 110
Size: 3359 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 422 1201 2086 422
Size: 779 885 3444
Fragments arranged by size:
3444 885 779
BstYI r'GATC_y
Cuts at: 1 2133 2600 2623 2958 3204 4216 4302 4314
Size: 2132 467 23 335 246 1012 86 12
Cuts at: 4314 4660 1
Size: 346 449
Fragments arranged by size:
2132 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 177 302 385 450 606 612 624 1906 177
Size: 125 83 65 156 6 12 1282 3379
Fragments arranged by size:
3379 1282 156 125 83 65 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3058 3239 3402 3058
Size: 181 163 4764
Fragments arranged by size:
4764 181 163
BtsI GCAGTG_nn'
Cuts at: 600 1127 2351 600
Size: 527 1224 3357
Fragments arranged by size:
3357 1224 527
DraI TTT'AAA
Cuts at: 2107 2107
Size: 5108
DrdI GACnn_nn'nnGTC
Cuts at: 2814 3672 2814
Size: 858 4250
EaeI y'GGCC_r
Cuts at: 441 609 1109 2693 2867 3258 3285 4630 441
Size: 168 500 1584 174 391 27 1345 919
Fragments arranged by size:
1584 1345 919 500 391 174 168 27
EagI C'GGCC_G
Cuts at: 441 609 2693 441
Size: 168 2084 2856
Fragments arranged by size:
2856 2084 168
EarI CTCTTCn'nnn_
Cuts at: 1332 1588 1922 3131 3341 1332
Size: 256 334 1209 210 3099
Fragments arranged by size:
3099 1209 334 256 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3316 3626 3772 4918 3316
Size: 310 146 1146 3506
Fragments arranged by size:
3506 1146 310 146
EcoICRI GAG'CTC
Cuts at: 588 2120 588
Size: 1532 3576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 1983 2933 3365 4112 4380 1946
Size: 37 950 432 747 268 2674
Fragments arranged by size:
2674 950 747 432 268 37
EcoNI CCTnn'n_nnAGG
Cuts at: 1630 1630
Size: 5108
EcoO109I rG'GnC_Cy
Cuts at: 208 298 630 813 1569 2247 208
Size: 90 332 183 756 678 3069
Fragments arranged by size:
3069 756 678 332 183 90
EcoRI G'AATT_C
Cuts at: 1915 2112 1915
Size: 197 4911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 882 1665 1935 3127 882
Size: 783 270 1192 2863
Fragments arranged by size:
2863 1192 783 270
FseI GG_CCGG'CC
Cuts at: 312 312
Size: 5108
FspI TGC'GCA
Cuts at: 2889 2889
Size: 5108
HaeII r_GCGC'y
Cuts at: 142 234 477 488 2542 2790 3812 4791 142
Size: 92 243 11 2054 248 1022 979 459
Fragments arranged by size:
2054 1022 979 459 248 243 92 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 960 992 1059 1091 1419 1451 2950
Size: 32 800 32 67 32 328 32 1499
Cuts at: 2950 2980 2982 3012 128
Size: 30 2 30 2224
Fragments arranged by size:
2224 1499 800 328 67 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1078 1389 2147 1078
Size: 311 758 4039
Fragments arranged by size:
4039 758 311
KasI G'GCGC_C
Cuts at: 484 2538 2786 484
Size: 2054 248 2806
Fragments arranged by size:
2806 2054 248
KpnI G_GTAC'C
Cuts at: 2128 2128
Size: 5108
MluI A'CGCG_T
Cuts at: 4541 4541
Size: 5108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1182 2030 3779 3963 1182
Size: 848 1749 184 2327
Fragments arranged by size:
2327 1749 848 184
MscI TGG'CCA
Cuts at: 1111 2869 1111
Size: 1758 3350
MslI CAynn'nnrTG
Cuts at: 1101 2062 3097 3224 1101
Size: 961 1035 127 2985
Fragments arranged by size:
2985 1035 961 127
MspA1I CmG'CkG
Cuts at: 387 452 482 501 608 626 1036 1413 1563
Size: 65 30 19 107 18 410 377 150
Cuts at: 1563 2219 2533 2893 3906 4151 387
Size: 656 314 360 1013 245 1344
Fragments arranged by size:
1344 1013 656 410 377 360 314 245
150 107 65 30 19 18
NaeI GCC'GGC
Cuts at: 310 3289 310
Size: 2979 2129
NarI GG'CG_CC
Cuts at: 485 2539 2787 485
Size: 2054 248 2806
Fragments arranged by size:
2806 2054 248
NcoI C'CATG_G
Cuts at: 177 1906 177
Size: 1729 3379
NdeI CA'TA_TG
Cuts at: 1519 1519
Size: 5108
NgoMIV G'CCGG_C
Cuts at: 308 3287 308
Size: 2979 2129
NsiI A_TGCA'T
Cuts at: 693 693
Size: 5108
NspI r_CATG'y
Cuts at: 537 1031 1401 1854 2067 2161 2661 3568 537
Size: 494 370 453 213 94 500 907 2077
Fragments arranged by size:
2077 907 500 494 453 370 213 94
PciI A'CATG_T
Cuts at: 1397 1850 3564 1397
Size: 453 1714 2941
Fragments arranged by size:
2941 1714 453
PflMI CCAn_nnn'nTGG
Cuts at: 131 924 131
Size: 793 4315
PmeI GTTT'AAAC
Cuts at: 2107 2107
Size: 5108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 5076
PpuMI rG'GwC_Cy
Cuts at: 630 813 630
Size: 183 4925
PspOMI G'GGCC_C
Cuts at: 208 298 616 208
Size: 90 318 4700
Fragments arranged by size:
4700 318 90
PstI C_TGCA'G
Cuts at: 344 1442 1463 1568 1682 2155 2840 344
Size: 1098 21 105 114 473 685 2612
Fragments arranged by size:
2612 1098 685 473 114 105 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5108
PvuII CAG'CTG
Cuts at: 501 1413 1563 2533 2893 501
Size: 912 150 970 360 2716
Fragments arranged by size:
2716 970 912 360 150
RsrII CG'GwC_CG
Cuts at: 3303 3303
Size: 5108
SacI G_AGCT'C
Cuts at: 590 2122 590
Size: 1532 3576
SacII CC_GC'GG
Cuts at: 388 453 609 627 388
Size: 65 156 18 4869
Fragments arranged by size:
4869 156 65 18
SalI G'TCGA_C
Cuts at: 1076 2145 1076
Size: 1069 4039
SanDI GG'GwC_CC
Cuts at: 630 630
Size: 5108
SapI GCTCTTCn'nnn_
Cuts at: 3131 3341 3131
Size: 210 4898
SbfI CC_TGCA'GG
Cuts at: 2155 2155
Size: 5108
ScaI AGT'ACT
Cuts at: 4625 4625
Size: 5108
SfcI C'TryA_G
Cuts at: 33 340 477 1438 1459 1564 1678 2151 2836
Size: 307 137 961 21 105 114 473 685
Cuts at: 2836 3829 4020 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 961 685 473 307 191 137
114 105 21
SfoI GGC'GCC
Cuts at: 486 2540 2788 486
Size: 2054 248 2806
Fragments arranged by size:
2806 2054 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
SmaI CCC'GGG
Cuts at: 2130 2130
Size: 5108
SmlI C'TyrA_G
Cuts at: 1332 2263 3178 3670 3932 1332
Size: 931 915 492 262 2508
Fragments arranged by size:
2508 931 915 492 262
SnaBI TAC'GTA
Cuts at: 2100 2100
Size: 5108
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5108
SphI G_CATG'C
Cuts at: 2161 2661 2161
Size: 500 4608
SspI AAT'ATT
Cuts at: 1795 1795
Size: 5108
StuI AGG'CCT
Cuts at: 1444 1444
Size: 5108
StyI C'CwwG_G
Cuts at: 177 1906 1944 2242 2596 177
Size: 1729 38 298 354 2689
Fragments arranged by size:
2689 1729 354 298 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 1210 2691 2824 3283 3492 1095
Size: 115 1481 133 459 209 2711
Fragments arranged by size:
2711 1481 459 209 133 115
TatI w'GTAC_w
Cuts at: 657 1694 1827 4623 657
Size: 1037 133 2796 1142
Fragments arranged by size:
2796 1142 1037 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1448 1480 1943 1975 4625 4657 1448
Size: 32 463 32 2650 32 1899
Fragments arranged by size:
2650 1899 463 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2905 2905
Size: 5108
XbaI T'CTAG_A
Cuts at: 2139 2139
Size: 5108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 5108
XmaI C'CCGG_G
Cuts at: 2128 2128
Size: 5108
XmnI GAAnn'nnTTC
Cuts at: 1399 1648 1915 1399
Size: 249 267 4592
Fragments arranged by size:
4592 267 249
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AloI AscI AvrII BaeI BbvCI
BcgI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PasI PfoI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SrfI SwaI XhoI ZraI