(Circular) (Six-base) MAPSORT of: pF1KSDA0214.seq Check: 6327 from: 1 to: 5408 pF1KSDA0214 5408 bp With 182 enzymes: SgfI * June 20, 2008 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1401 1401 Size: 5408 AatII G_ACGT'C Cuts at: 359 2240 359 Size: 1881 3527 AccI GT'mk_AC Cuts at: 1417 2446 1417 Size: 1029 4379 Acc65I G'GTAC_C Cuts at: 2424 2424 Size: 5408 AclI AA'CG_TT Cuts at: 5248 5248 Size: 5408 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 718 1109 2290 3233 3665 4412 4680 718 Size: 391 1181 943 432 747 268 1446 Fragments arranged by size: 1446 1181 943 747 432 391 268 AflII C'TTAA_G Cuts at: 211 211 Size: 5408 AflIII A'CryG_T Cuts at: 302 1556 2363 2760 3864 4841 302 Size: 1254 807 397 1104 977 869 Fragments arranged by size: 1254 1104 977 869 807 397 AgeI A'CCGG_T Cuts at: 3796 3796 Size: 5408 AleI CACnn'nnGTG Cuts at: 1683 1683 Size: 5408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 662 2282 662 Size: 1620 3788 AlwNI CAG_nnn'CTG Cuts at: 351 2199 2382 2713 4280 351 Size: 1848 183 331 1567 1479 Fragments arranged by size: 1848 1567 1479 331 183 ApaI G_GGCC'C Cuts at: 1801 1801 Size: 5408 ApaLI G'TGCA_C Cuts at: 4178 4178 Size: 5408 ApoI r'AATT_y Cuts at: 1143 1330 2412 4761 5017 1143 Size: 187 1082 2349 256 1534 Fragments arranged by size: 2349 1534 1082 256 187 AseI AT'TA_AT Cuts at: 20 20 Size: 5408 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5408 AvaI C'yCGr_G Cuts at: 925 1274 2243 2428 925 Size: 349 969 185 3905 Fragments arranged by size: 3905 969 349 185 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2362 2395 2362 Size: 33 5375 BamHI G'GATC_C Cuts at: 2433 2433 Size: 5408 BanI G'GyrC_C Cuts at: 1442 2424 2838 3086 3121 1442 Size: 982 414 248 35 3729 Fragments arranged by size: 3729 982 414 248 35 BanII G_rGCy'C Cuts at: 577 926 1355 1801 1903 2045 2360 2422 3452 Size: 349 429 446 102 142 315 62 1030 Cuts at: 3452 577 Size: 2533 Fragments arranged by size: 2533 1030 446 429 349 315 142 102 62 BbeI G_GCGC'C Cuts at: 1446 2842 3090 1446 Size: 1396 248 3764 Fragments arranged by size: 3764 1396 248 BbsI GAAGACnn'nnnn_ Cuts at: 305 1195 1748 305 Size: 890 553 3965 Fragments arranged by size: 3965 890 553 BbvCI CC'TCA_GC Cuts at: 191 191 Size: 5408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 138 172 138 Size: 34 5374 BciVI GTATCCnnnnn_n' Cuts at: 3306 4067 5408 3306 Size: 761 1341 3306 Fragments arranged by size: 3306 1341 761 BclI T'GATC_A Cuts at: 461 2928 461 Size: 2467 2941 BfrBI ATG'CAT Cuts at: 202 910 202 Size: 708 4700 BglII A'GATC_T Cuts at: 2923 4960 2923 Size: 2037 3371 BlpI GC'TnA_GC Cuts at: 2520 2520 Size: 5408 Bme1580I G_kGCm'C Cuts at: 541 691 1801 3033 3126 4182 541 Size: 150 1110 1232 93 1056 1767 Fragments arranged by size: 1767 1232 1110 1056 150 93 BmrI ACTGGGnnnn_n' Cuts at: 1170 1555 2620 2701 3036 5180 1170 Size: 385 1065 81 335 2144 1398 Fragments arranged by size: 2144 1398 1065 385 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 885 1188 3775 885 Size: 303 2587 2518 Fragments arranged by size: 2587 2518 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1369 1475 2275 1369 Size: 106 800 4502 Fragments arranged by size: 4502 800 106 Bpu10I CC'TnA_GC Cuts at: 191 1043 2326 191 Size: 852 1283 3273 Fragments arranged by size: 3273 1283 852 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1346 1486 2584 3463 3955 4253 1346 Size: 140 1098 879 492 298 2501 Fragments arranged by size: 2501 1098 879 492 298 140 BsaI GGTCTCn'nnnn_ Cuts at: 5112 5112 Size: 5408 BsaAI yAC'GTr Cuts at: 305 2400 2761 305 Size: 2095 361 2952 Fragments arranged by size: 2952 2095 361 BsaBI GATnn'nnATC Cuts at: 6 2922 4959 6 Size: 2916 2037 455 Fragments arranged by size: 2916 2037 455 BsaHI Gr'CG_yC Cuts at: 356 1443 1864 2237 2839 3087 3789 5285 356 Size: 1087 421 373 602 248 702 1496 479 Fragments arranged by size: 1496 1087 702 602 479 421 373 248 BsaWI w'CCGG_w Cuts at: 2597 3118 3796 4070 4217 4628 2597 Size: 521 678 274 147 411 3377 Fragments arranged by size: 3377 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1555 1585 1982 2012 2152 2182 1555 Size: 30 397 30 140 30 4781 Fragments arranged by size: 4781 397 140 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 603 952 1167 1177 1447 1930 2006 2044 603 Size: 349 215 10 270 483 76 38 3967 Fragments arranged by size: 3967 483 349 270 215 76 38 10 BseYI C'CCAG_C Cuts at: 261 902 990 1876 2389 2833 3591 4168 4664 Size: 641 88 886 513 444 758 577 496 Cuts at: 4664 261 Size: 1005 Fragments arranged by size: 1005 886 758 641 577 513 496 444 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1246 1507 1246 Size: 261 5147 BsiEI CG_ry'CG Cuts at: 104 2950 2996 4204 104 Size: 2846 46 1208 1308 Fragments arranged by size: 2846 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 457 1055 1355 2360 2422 3200 3390 4182 457 Size: 598 300 1005 62 778 190 792 1683 Fragments arranged by size: 1683 1005 792 778 598 300 190 62 BsmI GAATG_Cn' Cuts at: 204 318 1976 204 Size: 114 1658 3636 Fragments arranged by size: 3636 1658 114 BsmBI CGTCTCn'nnnn_ Cuts at: 871 871 Size: 5408 Bsp1286I G_dGCh'C Cuts at: 457 541 577 691 926 1055 1355 1801 1903 Size: 84 36 114 235 129 300 446 102 Cuts at: 1903 2045 2360 2422 3033 3126 3200 3390 3452 Size: 142 315 62 611 93 74 190 62 Cuts at: 3452 4182 457 Size: 730 1683 Fragments arranged by size: 1683 730 611 446 315 300 235 190 142 129 114 102 93 84 74 62 62 36 BspEI T'CCGG_A Cuts at: 4628 4628 Size: 5408 BspHI T'CATG_A Cuts at: 4584 4584 Size: 5408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1275 1401 2390 2458 2672 2974 3355 1275 Size: 126 989 68 214 302 381 3328 Fragments arranged by size: 3328 989 381 302 214 126 68 BsrBI CCG'CTC Cuts at: 50 1314 3700 3754 5238 5403 50 Size: 1264 2386 54 1484 165 55 Fragments arranged by size: 2386 1484 1264 165 55 54 BsrDI GCAATG_nn' Cuts at: 450 1430 2024 3320 450 Size: 980 594 1296 2538 Fragments arranged by size: 2538 1296 980 594 BsrFI r'CCGG_y Cuts at: 649 1939 2338 3406 3587 3796 649 Size: 1290 399 1068 181 209 2261 Fragments arranged by size: 2261 1290 1068 399 209 181 BssSI C'ACGA_G Cuts at: 3679 4037 3679 Size: 358 5050 BstAPI GCAn_nnn'nTGC Cuts at: 1602 2785 1602 Size: 1183 4225 BstBI TT'CG_AA Cuts at: 110 3769 110 Size: 3659 1749 BstEII G'GTnAC_C Cuts at: 364 1935 364 Size: 1571 3837 BstXI CCAn_nnnn'nTGG Cuts at: 750 978 750 Size: 228 5180 BstYI r'GATC_y Cuts at: 1 2433 2900 2923 3258 3504 4516 4602 4614 Size: 2432 467 23 335 246 1012 86 12 Cuts at: 4614 4960 1 Size: 346 449 Fragments arranged by size: 2432 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 1574 1931 1970 1574 Size: 357 39 5012 Fragments arranged by size: 5012 357 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3358 3539 3702 3358 Size: 181 163 5064 Fragments arranged by size: 5064 181 163 BtsI GCAGTG_nn' Cuts at: 224 1190 1888 2015 2651 224 Size: 966 698 127 636 2981 Fragments arranged by size: 2981 966 698 636 127 ClaI AT'CG_AT Cuts at: 1005 1005 Size: 5408 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 276 311 276 Size: 35 5373 DraI TTT'AAA Cuts at: 2407 2407 Size: 5408 DraIII CAC_nnn'GTG Cuts at: 855 855 Size: 5408 DrdI GACnn_nn'nnGTC Cuts at: 1672 3114 3972 1672 Size: 1442 858 3108 Fragments arranged by size: 3108 1442 858 EaeI y'GGCC_r Cuts at: 432 504 546 803 874 1427 1577 2993 3167 Size: 72 42 257 71 553 150 1416 174 Cuts at: 3167 3558 3585 4930 432 Size: 391 27 1345 910 Fragments arranged by size: 1416 1345 910 553 391 257 174 150 72 71 42 27 EagI C'GGCC_G Cuts at: 2993 2993 Size: 5408 EarI CTCTTCn'nnn_ Cuts at: 142 445 1304 3431 3641 142 Size: 303 859 2127 210 1909 Fragments arranged by size: 2127 1909 859 303 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3616 3926 4072 5218 3616 Size: 310 146 1146 3806 Fragments arranged by size: 3806 1146 310 146 EcoICRI GAG'CTC Cuts at: 1353 2358 2420 1353 Size: 1005 62 4341 Fragments arranged by size: 4341 1005 62 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 718 1109 1369 1475 2275 2290 3233 3665 4412 Size: 391 260 106 800 15 943 432 747 Cuts at: 4412 4680 718 Size: 268 1446 Fragments arranged by size: 1446 943 800 747 432 391 268 260 106 15 EcoNI CCTnn'n_nnAGG Cuts at: 568 628 1091 2257 568 Size: 60 463 1166 3719 Fragments arranged by size: 3719 1166 463 60 EcoO109I rG'GnC_Cy Cuts at: 266 1798 1964 2102 2207 2547 266 Size: 1532 166 138 105 340 3127 Fragments arranged by size: 3127 1532 340 166 138 105 EcoRI G'AATT_C Cuts at: 2412 2412 Size: 5408 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 861 3427 861 Size: 2566 2842 FspI TGC'GCA Cuts at: 3189 3189 Size: 5408 HaeII r_GCGC'y Cuts at: 287 1103 1446 2115 2842 3090 4112 5091 287 Size: 816 343 669 727 248 1022 979 604 Fragments arranged by size: 1022 979 816 727 669 604 343 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 849 881 1555 1587 2199 2231 3250 3280 3282 Size: 32 674 32 612 32 1019 30 2 Cuts at: 3282 3312 849 Size: 30 2945 Fragments arranged by size: 2945 1019 674 612 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1418 2235 2447 1418 Size: 817 212 4379 Fragments arranged by size: 4379 817 212 KasI G'GCGC_C Cuts at: 1442 2838 3086 1442 Size: 1396 248 3764 Fragments arranged by size: 3764 1396 248 KpnI G_GTAC'C Cuts at: 2428 2428 Size: 5408 MfeI C'AATT_G Cuts at: 516 516 Size: 5408 MluI A'CGCG_T Cuts at: 4841 4841 Size: 5408 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1968 2195 2327 4079 4263 1968 Size: 227 132 1752 184 3113 Fragments arranged by size: 3113 1752 227 184 132 MscI TGG'CCA Cuts at: 506 548 805 3169 506 Size: 42 257 2364 2745 Fragments arranged by size: 2745 2364 257 42 MslI CAynn'nnrTG Cuts at: 418 823 1683 1780 3397 3524 418 Size: 405 860 97 1617 127 2302 Fragments arranged by size: 2302 1617 860 405 127 97 MspA1I CmG'CkG Cuts at: 261 612 758 902 1347 1568 2519 2833 3193 Size: 351 146 144 445 221 951 314 360 Cuts at: 3193 4206 4451 261 Size: 1013 245 1218 Fragments arranged by size: 1218 1013 951 445 360 351 314 245 221 146 144 NaeI GCC'GGC Cuts at: 651 3589 651 Size: 2938 2470 NarI GG'CG_CC Cuts at: 1443 2839 3087 1443 Size: 1396 248 3764 Fragments arranged by size: 3764 1396 248 NcoI C'CATG_G Cuts at: 1931 1970 1931 Size: 39 5369 NgoMIV G'CCGG_C Cuts at: 649 3587 649 Size: 2938 2470 NsiI A_TGCA'T Cuts at: 204 912 204 Size: 708 4700 NspI r_CATG'y Cuts at: 1086 1560 1671 1884 2131 2367 2461 2961 3868 Size: 474 111 213 247 236 94 500 907 Cuts at: 3868 1086 Size: 2626 Fragments arranged by size: 2626 907 500 474 247 236 213 111 94 PasI CC'CwG_GG Cuts at: 1077 1077 Size: 5408 PciI A'CATG_T Cuts at: 1556 2363 3864 1556 Size: 807 1501 3100 Fragments arranged by size: 3100 1501 807 PflMI CCAn_nnn'nTGG Cuts at: 476 2121 476 Size: 1645 3763 PfoI T'CCnGG_A Cuts at: 275 1736 1960 275 Size: 1461 224 3723 Fragments arranged by size: 3723 1461 224 PmeI GTTT'AAAC Cuts at: 2407 2407 Size: 5408 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4547 4579 4547 Size: 32 5376 PpuMI rG'GwC_Cy Cuts at: 1964 2207 1964 Size: 243 5165 PsiI TTA'TAA Cuts at: 1511 1511 Size: 5408 PspOMI G'GGCC_C Cuts at: 1797 1797 Size: 5408 PstI C_TGCA'G Cuts at: 1185 1598 2147 2179 2387 2455 3140 1185 Size: 413 549 32 208 68 685 3453 Fragments arranged by size: 3453 685 549 413 208 68 32 PvuI CG_AT'CG Cuts at: 104 104 Size: 5408 PvuII CAG'CTG Cuts at: 261 612 758 1347 2833 3193 261 Size: 351 146 589 1486 360 2476 Fragments arranged by size: 2476 1486 589 360 351 146 RsrII CG'GwC_CG Cuts at: 1212 3603 1212 Size: 2391 3017 SacI G_AGCT'C Cuts at: 1355 2360 2422 1355 Size: 1005 62 4341 Fragments arranged by size: 4341 1005 62 SalI G'TCGA_C Cuts at: 1416 2445 1416 Size: 1029 4379 SapI GCTCTTCn'nnn_ Cuts at: 142 445 1304 3431 3641 142 Size: 303 859 2127 210 1909 Fragments arranged by size: 2127 1909 859 303 210 SbfI CC_TGCA'GG Cuts at: 2455 2455 Size: 5408 ScaI AGT'ACT Cuts at: 4925 4925 Size: 5408 SexAI A'CCwGG_T Cuts at: 1838 1838 Size: 5408 SfcI C'TryA_G Cuts at: 33 1181 1594 2143 2175 2383 2451 3136 4129 Size: 1148 413 549 32 208 68 685 993 Cuts at: 4129 4320 33 Size: 191 1121 Fragments arranged by size: 1148 1121 993 685 549 413 208 191 68 32 SfoI GGC'GCC Cuts at: 1444 2840 3088 1444 Size: 1396 248 3764 Fragments arranged by size: 3764 1396 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5408 SmaI CCC'GGG Cuts at: 2245 2430 2245 Size: 185 5223 SmlI C'TyrA_G Cuts at: 211 1361 1501 2563 3478 3970 4232 211 Size: 1150 140 1062 915 492 262 1387 Fragments arranged by size: 1387 1150 1062 915 492 262 140 SnaBI TAC'GTA Cuts at: 2400 2400 Size: 5408 SpeI A'CTAG_T Cuts at: 67 67 Size: 5408 SphI G_CATG'C Cuts at: 1086 1884 2131 2461 2961 1086 Size: 798 247 330 500 3533 Fragments arranged by size: 3533 798 500 330 247 StuI AGG'CCT Cuts at: 556 556 Size: 5408 StyI C'CwwG_G Cuts at: 1931 1970 2087 2093 2542 2896 1931 Size: 39 117 6 449 354 4443 Fragments arranged by size: 4443 449 354 117 39 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1337 1924 2991 3124 3583 3792 1337 Size: 587 1067 133 459 209 2953 Fragments arranged by size: 2953 1067 587 459 209 133 TatI w'GTAC_w Cuts at: 929 1452 4923 929 Size: 523 3471 1414 Fragments arranged by size: 3471 1414 523 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1982 2014 4925 4957 1982 Size: 32 2911 32 2433 Fragments arranged by size: 2911 2433 32 32 Tth111I GACn'n_nGTC Cuts at: 1547 1840 3205 1547 Size: 293 1365 3750 Fragments arranged by size: 3750 1365 293 XbaI T'CTAG_A Cuts at: 2439 2439 Size: 5408 XcmI CCAnnnn_n'nnnnTGG Cuts at: 978 1968 2903 978 Size: 990 935 3483 Fragments arranged by size: 3483 990 935 XmaI C'CCGG_G Cuts at: 2243 2428 2243 Size: 185 5223 XmnI GAAnn'nnTTC Cuts at: 347 1115 1789 347 Size: 768 674 3966 Fragments arranged by size: 3966 768 674 ZraI GAC'GTC Cuts at: 357 2238 357 Size: 1881 3527 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AfeI AhdI AloI AscI AvrII BglI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI PacI PmlI PshAI PspXI PsrI SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI