Restriction map for pF1KSDA0214
 (Circular) (Six-base) MAPSORT of: pF1KSDA0214.seq  Check: 6327  from: 1  to: 5408



pF1KSDA0214  5408 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1401    1401 
   Size:       5408
 
AatII G_ACGT'C

Cuts at:    359    2240     359 
   Size:       1881    3527
 
AccI GT'mk_AC

Cuts at:   1417    2446    1417 
   Size:       1029    4379
 
Acc65I G'GTAC_C

Cuts at:   2424    2424 
   Size:       5408
 
AclI AA'CG_TT

Cuts at:   5248    5248 
   Size:       5408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    718    1109    2290    3233    3665    4412    4680     718 
   Size:        391    1181     943     432     747     268    1446

  Fragments arranged by size: 

                1446    1181     943     747     432     391     268
 
AflII C'TTAA_G

Cuts at:    211     211 
   Size:       5408
 
AflIII A'CryG_T

Cuts at:    302    1556    2363    2760    3864    4841     302 
   Size:       1254     807     397    1104     977     869

  Fragments arranged by size: 

                1254    1104     977     869     807     397
 
AgeI A'CCGG_T

Cuts at:   3796    3796 
   Size:       5408
 
AleI CACnn'nnGTG

Cuts at:   1683    1683 
   Size:       5408
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    662    2282     662 
   Size:       1620    3788
 
AlwNI CAG_nnn'CTG

Cuts at:    351    2199    2382    2713    4280     351 
   Size:       1848     183     331    1567    1479

  Fragments arranged by size: 

                1848    1567    1479     331     183
 
ApaI G_GGCC'C

Cuts at:   1801    1801 
   Size:       5408
 
ApaLI G'TGCA_C

Cuts at:   4178    4178 
   Size:       5408
 
ApoI r'AATT_y

Cuts at:   1143    1330    2412    4761    5017    1143 
   Size:        187    1082    2349     256    1534

  Fragments arranged by size: 

                2349    1534    1082     256     187
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5408
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5408
 
AvaI C'yCGr_G

Cuts at:    925    1274    2243    2428     925 
   Size:        349     969     185    3905

  Fragments arranged by size: 

                3905     969     349     185
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2362    2395    2362 
   Size:         33    5375
 
BamHI G'GATC_C

Cuts at:   2433    2433 
   Size:       5408
 
BanI G'GyrC_C

Cuts at:   1442    2424    2838    3086    3121    1442 
   Size:        982     414     248      35    3729

  Fragments arranged by size: 

                3729     982     414     248      35
 
BanII G_rGCy'C

Cuts at:    577     926    1355    1801    1903    2045    2360    2422    3452 
   Size:        349     429     446     102     142     315      62    1030

Cuts at:   3452     577 
   Size:       2533

  Fragments arranged by size: 

                2533    1030     446     429     349     315     142     102
                  62
 
BbeI G_GCGC'C

Cuts at:   1446    2842    3090    1446 
   Size:       1396     248    3764

  Fragments arranged by size: 

                3764    1396     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    305    1195    1748     305 
   Size:        890     553    3965

  Fragments arranged by size: 

                3965     890     553
 
BbvCI CC'TCA_GC

Cuts at:    191     191 
   Size:       5408
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    138     172     138 
   Size:         34    5374
 
BciVI GTATCCnnnnn_n'

Cuts at:   3306    4067    5408    3306 
   Size:        761    1341    3306

  Fragments arranged by size: 

                3306    1341     761
 
BclI T'GATC_A

Cuts at:    461    2928     461 
   Size:       2467    2941
 
BfrBI ATG'CAT

Cuts at:    202     910     202 
   Size:        708    4700
 
BglII A'GATC_T

Cuts at:   2923    4960    2923 
   Size:       2037    3371
 
BlpI GC'TnA_GC

Cuts at:   2520    2520 
   Size:       5408
 
Bme1580I G_kGCm'C

Cuts at:    541     691    1801    3033    3126    4182     541 
   Size:        150    1110    1232      93    1056    1767

  Fragments arranged by size: 

                1767    1232    1110    1056     150      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1170    1555    2620    2701    3036    5180    1170 
   Size:        385    1065      81     335    2144    1398

  Fragments arranged by size: 

                2144    1398    1065     385     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    885    1188    3775     885 
   Size:        303    2587    2518

  Fragments arranged by size: 

                2587    2518     303
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1369    1475    2275    1369 
   Size:        106     800    4502

  Fragments arranged by size: 

                4502     800     106
 
Bpu10I CC'TnA_GC

Cuts at:    191    1043    2326     191 
   Size:        852    1283    3273

  Fragments arranged by size: 

                3273    1283     852
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1346    1486    2584    3463    3955    4253    1346 
   Size:        140    1098     879     492     298    2501

  Fragments arranged by size: 

                2501    1098     879     492     298     140
 
BsaI GGTCTCn'nnnn_

Cuts at:   5112    5112 
   Size:       5408
 
BsaAI yAC'GTr

Cuts at:    305    2400    2761     305 
   Size:       2095     361    2952

  Fragments arranged by size: 

                2952    2095     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2922    4959       6 
   Size:       2916    2037     455

  Fragments arranged by size: 

                2916    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    356    1443    1864    2237    2839    3087    3789    5285     356 
   Size:       1087     421     373     602     248     702    1496     479

  Fragments arranged by size: 

                1496    1087     702     602     479     421     373     248
            
 
BsaWI w'CCGG_w

Cuts at:   2597    3118    3796    4070    4217    4628    2597 
   Size:        521     678     274     147     411    3377

  Fragments arranged by size: 

                3377     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1555    1585    1982    2012    2152    2182    1555 
   Size:         30     397      30     140      30    4781

  Fragments arranged by size: 

                4781     397     140      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    603     952    1167    1177    1447    1930    2006    2044     603 
   Size:        349     215      10     270     483      76      38    3967

  Fragments arranged by size: 

                3967     483     349     270     215      76      38      10
            
 
BseYI C'CCAG_C

Cuts at:    261     902     990    1876    2389    2833    3591    4168    4664 
   Size:        641      88     886     513     444     758     577     496

Cuts at:   4664     261 
   Size:       1005

  Fragments arranged by size: 

                1005     886     758     641     577     513     496     444
                  88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1246    1507    1246 
   Size:        261    5147
 
BsiEI CG_ry'CG

Cuts at:    104    2950    2996    4204     104 
   Size:       2846      46    1208    1308

  Fragments arranged by size: 

                2846    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    457    1055    1355    2360    2422    3200    3390    4182     457 
   Size:        598     300    1005      62     778     190     792    1683

  Fragments arranged by size: 

                1683    1005     792     778     598     300     190      62
            
 
BsmI GAATG_Cn'

Cuts at:    204     318    1976     204 
   Size:        114    1658    3636

  Fragments arranged by size: 

                3636    1658     114
 
BsmBI CGTCTCn'nnnn_

Cuts at:    871     871 
   Size:       5408
 
Bsp1286I G_dGCh'C

Cuts at:    457     541     577     691     926    1055    1355    1801    1903 
   Size:         84      36     114     235     129     300     446     102

Cuts at:   1903    2045    2360    2422    3033    3126    3200    3390    3452 
   Size:        142     315      62     611      93      74     190      62

Cuts at:   3452    4182     457 
   Size:        730    1683

  Fragments arranged by size: 

                1683     730     611     446     315     300     235     190
                 142     129     114     102      93      84      74      62
                  62      36
 
BspEI T'CCGG_A

Cuts at:   4628    4628 
   Size:       5408
 
BspHI T'CATG_A

Cuts at:   4584    4584 
   Size:       5408
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1275    1401    2390    2458    2672    2974    3355    1275 
   Size:        126     989      68     214     302     381    3328

  Fragments arranged by size: 

                3328     989     381     302     214     126      68
 
BsrBI CCG'CTC

Cuts at:     50    1314    3700    3754    5238    5403      50 
   Size:       1264    2386      54    1484     165      55

  Fragments arranged by size: 

                2386    1484    1264     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    450    1430    2024    3320     450 
   Size:        980     594    1296    2538

  Fragments arranged by size: 

                2538    1296     980     594
 
BsrFI r'CCGG_y

Cuts at:    649    1939    2338    3406    3587    3796     649 
   Size:       1290     399    1068     181     209    2261

  Fragments arranged by size: 

                2261    1290    1068     399     209     181
 
BssSI C'ACGA_G

Cuts at:   3679    4037    3679 
   Size:        358    5050
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1602    2785    1602 
   Size:       1183    4225
 
BstBI TT'CG_AA

Cuts at:    110    3769     110 
   Size:       3659    1749
 
BstEII G'GTnAC_C

Cuts at:    364    1935     364 
   Size:       1571    3837
 
BstXI CCAn_nnnn'nTGG

Cuts at:    750     978     750 
   Size:        228    5180
 
BstYI r'GATC_y

Cuts at:      1    2433    2900    2923    3258    3504    4516    4602    4614 
   Size:       2432     467      23     335     246    1012      86      12

Cuts at:   4614    4960       1 
   Size:        346     449

  Fragments arranged by size: 

                2432    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:   1574    1931    1970    1574 
   Size:        357      39    5012

  Fragments arranged by size: 

                5012     357      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3358    3539    3702    3358 
   Size:        181     163    5064

  Fragments arranged by size: 

                5064     181     163
 
BtsI GCAGTG_nn'

Cuts at:    224    1190    1888    2015    2651     224 
   Size:        966     698     127     636    2981

  Fragments arranged by size: 

                2981     966     698     636     127
 
ClaI AT'CG_AT

Cuts at:   1005    1005 
   Size:       5408
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    276     311     276 
   Size:         35    5373
 
DraI TTT'AAA

Cuts at:   2407    2407 
   Size:       5408
 
DraIII CAC_nnn'GTG

Cuts at:    855     855 
   Size:       5408
 
DrdI GACnn_nn'nnGTC

Cuts at:   1672    3114    3972    1672 
   Size:       1442     858    3108

  Fragments arranged by size: 

                3108    1442     858
 
EaeI y'GGCC_r

Cuts at:    432     504     546     803     874    1427    1577    2993    3167 
   Size:         72      42     257      71     553     150    1416     174

Cuts at:   3167    3558    3585    4930     432 
   Size:        391      27    1345     910

  Fragments arranged by size: 

                1416    1345     910     553     391     257     174     150
                  72      71      42      27
 
EagI C'GGCC_G

Cuts at:   2993    2993 
   Size:       5408
 
EarI CTCTTCn'nnn_

Cuts at:    142     445    1304    3431    3641     142 
   Size:        303     859    2127     210    1909

  Fragments arranged by size: 

                2127    1909     859     303     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3616    3926    4072    5218    3616 
   Size:        310     146    1146    3806

  Fragments arranged by size: 

                3806    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1353    2358    2420    1353 
   Size:       1005      62    4341

  Fragments arranged by size: 

                4341    1005      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    718    1109    1369    1475    2275    2290    3233    3665    4412 
   Size:        391     260     106     800      15     943     432     747

Cuts at:   4412    4680     718 
   Size:        268    1446

  Fragments arranged by size: 

                1446     943     800     747     432     391     268     260
                 106      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:    568     628    1091    2257     568 
   Size:         60     463    1166    3719

  Fragments arranged by size: 

                3719    1166     463      60
 
EcoO109I rG'GnC_Cy

Cuts at:    266    1798    1964    2102    2207    2547     266 
   Size:       1532     166     138     105     340    3127

  Fragments arranged by size: 

                3127    1532     340     166     138     105
 
EcoRI G'AATT_C

Cuts at:   2412    2412 
   Size:       5408
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    861    3427     861 
   Size:       2566    2842
 
FspI TGC'GCA

Cuts at:   3189    3189 
   Size:       5408
 
HaeII r_GCGC'y

Cuts at:    287    1103    1446    2115    2842    3090    4112    5091     287 
   Size:        816     343     669     727     248    1022     979     604

  Fragments arranged by size: 

                1022     979     816     727     669     604     343     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    849     881    1555    1587    2199    2231    3250    3280    3282 
   Size:         32     674      32     612      32    1019      30       2

Cuts at:   3282    3312     849 
   Size:         30    2945

  Fragments arranged by size: 

                2945    1019     674     612      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1418    2235    2447    1418 
   Size:        817     212    4379

  Fragments arranged by size: 

                4379     817     212
 
KasI G'GCGC_C

Cuts at:   1442    2838    3086    1442 
   Size:       1396     248    3764

  Fragments arranged by size: 

                3764    1396     248
 
KpnI G_GTAC'C

Cuts at:   2428    2428 
   Size:       5408
 
MfeI C'AATT_G

Cuts at:    516     516 
   Size:       5408
 
MluI A'CGCG_T

Cuts at:   4841    4841 
   Size:       5408
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1968    2195    2327    4079    4263    1968 
   Size:        227     132    1752     184    3113

  Fragments arranged by size: 

                3113    1752     227     184     132
 
MscI TGG'CCA

Cuts at:    506     548     805    3169     506 
   Size:         42     257    2364    2745

  Fragments arranged by size: 

                2745    2364     257      42
 
MslI CAynn'nnrTG

Cuts at:    418     823    1683    1780    3397    3524     418 
   Size:        405     860      97    1617     127    2302

  Fragments arranged by size: 

                2302    1617     860     405     127      97
 
MspA1I CmG'CkG

Cuts at:    261     612     758     902    1347    1568    2519    2833    3193 
   Size:        351     146     144     445     221     951     314     360

Cuts at:   3193    4206    4451     261 
   Size:       1013     245    1218

  Fragments arranged by size: 

                1218    1013     951     445     360     351     314     245
                 221     146     144
 
NaeI GCC'GGC

Cuts at:    651    3589     651 
   Size:       2938    2470
 
NarI GG'CG_CC

Cuts at:   1443    2839    3087    1443 
   Size:       1396     248    3764

  Fragments arranged by size: 

                3764    1396     248
 
NcoI C'CATG_G

Cuts at:   1931    1970    1931 
   Size:         39    5369
 
NgoMIV G'CCGG_C

Cuts at:    649    3587     649 
   Size:       2938    2470
 
NsiI A_TGCA'T

Cuts at:    204     912     204 
   Size:        708    4700
 
NspI r_CATG'y

Cuts at:   1086    1560    1671    1884    2131    2367    2461    2961    3868 
   Size:        474     111     213     247     236      94     500     907

Cuts at:   3868    1086 
   Size:       2626

  Fragments arranged by size: 

                2626     907     500     474     247     236     213     111
                  94
 
PasI CC'CwG_GG

Cuts at:   1077    1077 
   Size:       5408
 
PciI A'CATG_T

Cuts at:   1556    2363    3864    1556 
   Size:        807    1501    3100

  Fragments arranged by size: 

                3100    1501     807
 
PflMI CCAn_nnn'nTGG

Cuts at:    476    2121     476 
   Size:       1645    3763
 
PfoI T'CCnGG_A

Cuts at:    275    1736    1960     275 
   Size:       1461     224    3723

  Fragments arranged by size: 

                3723    1461     224
 
PmeI GTTT'AAAC

Cuts at:   2407    2407 
   Size:       5408
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4547    4579    4547 
   Size:         32    5376
 
PpuMI rG'GwC_Cy

Cuts at:   1964    2207    1964 
   Size:        243    5165
 
PsiI TTA'TAA

Cuts at:   1511    1511 
   Size:       5408
 
PspOMI G'GGCC_C

Cuts at:   1797    1797 
   Size:       5408
 
PstI C_TGCA'G

Cuts at:   1185    1598    2147    2179    2387    2455    3140    1185 
   Size:        413     549      32     208      68     685    3453

  Fragments arranged by size: 

                3453     685     549     413     208      68      32
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5408
 
PvuII CAG'CTG

Cuts at:    261     612     758    1347    2833    3193     261 
   Size:        351     146     589    1486     360    2476

  Fragments arranged by size: 

                2476    1486     589     360     351     146
 
RsrII CG'GwC_CG

Cuts at:   1212    3603    1212 
   Size:       2391    3017
 
SacI G_AGCT'C

Cuts at:   1355    2360    2422    1355 
   Size:       1005      62    4341

  Fragments arranged by size: 

                4341    1005      62
 
SalI G'TCGA_C

Cuts at:   1416    2445    1416 
   Size:       1029    4379
 
SapI GCTCTTCn'nnn_

Cuts at:    142     445    1304    3431    3641     142 
   Size:        303     859    2127     210    1909

  Fragments arranged by size: 

                2127    1909     859     303     210
 
SbfI CC_TGCA'GG

Cuts at:   2455    2455 
   Size:       5408
 
ScaI AGT'ACT

Cuts at:   4925    4925 
   Size:       5408
 
SexAI A'CCwGG_T

Cuts at:   1838    1838 
   Size:       5408
 
SfcI C'TryA_G

Cuts at:     33    1181    1594    2143    2175    2383    2451    3136    4129 
   Size:       1148     413     549      32     208      68     685     993

Cuts at:   4129    4320      33 
   Size:        191    1121

  Fragments arranged by size: 

                1148    1121     993     685     549     413     208     191
                  68      32
 
SfoI GGC'GCC

Cuts at:   1444    2840    3088    1444 
   Size:       1396     248    3764

  Fragments arranged by size: 

                3764    1396     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5408
 
SmaI CCC'GGG

Cuts at:   2245    2430    2245 
   Size:        185    5223
 
SmlI C'TyrA_G

Cuts at:    211    1361    1501    2563    3478    3970    4232     211 
   Size:       1150     140    1062     915     492     262    1387

  Fragments arranged by size: 

                1387    1150    1062     915     492     262     140
 
SnaBI TAC'GTA

Cuts at:   2400    2400 
   Size:       5408
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5408
 
SphI G_CATG'C

Cuts at:   1086    1884    2131    2461    2961    1086 
   Size:        798     247     330     500    3533

  Fragments arranged by size: 

                3533     798     500     330     247
 
StuI AGG'CCT

Cuts at:    556     556 
   Size:       5408
 
StyI C'CwwG_G

Cuts at:   1931    1970    2087    2093    2542    2896    1931 
   Size:         39     117       6     449     354    4443

  Fragments arranged by size: 

                4443     449     354     117      39       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1337    1924    2991    3124    3583    3792    1337 
   Size:        587    1067     133     459     209    2953

  Fragments arranged by size: 

                2953    1067     587     459     209     133
 
TatI w'GTAC_w

Cuts at:    929    1452    4923     929 
   Size:        523    3471    1414

  Fragments arranged by size: 

                3471    1414     523
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1982    2014    4925    4957    1982 
   Size:         32    2911      32    2433

  Fragments arranged by size: 

                2911    2433      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1547    1840    3205    1547 
   Size:        293    1365    3750

  Fragments arranged by size: 

                3750    1365     293
 
XbaI T'CTAG_A

Cuts at:   2439    2439 
   Size:       5408
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    978    1968    2903     978 
   Size:        990     935    3483

  Fragments arranged by size: 

                3483     990     935
 
XmaI C'CCGG_G

Cuts at:   2243    2428    2243 
   Size:        185    5223
 
XmnI GAAnn'nnTTC

Cuts at:    347    1115    1789     347 
   Size:        768     674    3966

  Fragments arranged by size: 

                3966     768     674
 
ZraI GAC'GTC

Cuts at:    357    2238     357 
   Size:       1881    3527
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AhdI     AloI     AscI    AvrII     BglI    BmgBI     BmtI
    BsiWI    BsrGI   BssHII  BstZ17I   Bsu36I    EcoRV     FseI    FspAI
  HindIII     HpaI     NdeI     NheI     NotI     NruI     PacI     PmlI
    PshAI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI     SrfI
     SspI     SwaI     XhoI
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