Restriction map for
pF1KSDA0215
(Circular) (Six-base) MAPSORT of: pF1KSDA0215.seq Check: 2910 from: 1 to: 5606
pF1KSDA0215 5606 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1854 1854
Size: 5606
AccI GT'mk_AC
Cuts at: 2370 2644 2370
Size: 274 5332
Acc65I G'GTAC_C
Cuts at: 369 2622 369
Size: 2253 3353
AclI AA'CG_TT
Cuts at: 5446 5446
Size: 5606
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 940 1453 1630 2035 3431 3863 4610 4878
Size: 387 513 177 405 1396 432 747 268
Cuts at: 4878 553
Size: 1281
Fragments arranged by size:
1396 1281 747 513 432 405 387 268
177
AflIII A'CryG_T
Cuts at: 2958 4062 5039 2958
Size: 1104 977 3525
Fragments arranged by size:
3525 1104 977
AgeI A'CCGG_T
Cuts at: 3994 3994
Size: 5606
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 546 546
Size: 5606
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1327 1359 2188 2220 1327
Size: 32 829 32 4713
Fragments arranged by size:
4713 829 32 32
AlwNI CAG_nnn'CTG
Cuts at: 493 526 1560 2031 2911 4478 493
Size: 33 1034 471 880 1567 1621
Fragments arranged by size:
1621 1567 1034 880 471 33
ApaLI G'TGCA_C
Cuts at: 4376 4376
Size: 5606
ApoI r'AATT_y
Cuts at: 221 2610 4959 5215 221
Size: 2389 2349 256 612
Fragments arranged by size:
2389 2349 612 256
AseI AT'TA_AT
Cuts at: 20 305 20
Size: 285 5321
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5606
AvaI C'yCGr_G
Cuts at: 2172 2626 2172
Size: 454 5152
BamHI G'GATC_C
Cuts at: 971 2631 971
Size: 1660 3946
BanI G'GyrC_C
Cuts at: 369 1866 2622 3036 3284 3319 369
Size: 1497 756 414 248 35 2656
Fragments arranged by size:
2656 1497 756 414 248 35
BanII G_rGCy'C
Cuts at: 918 951 2350 2403 2620 3650 918
Size: 33 1399 53 217 1030 2874
Fragments arranged by size:
2874 1399 1030 217 53 33
BbeI G_GCGC'C
Cuts at: 3040 3288 3040
Size: 248 5358
BbsI GAAGACnn'nnnn_
Cuts at: 590 928 1084 1171 1784 2066 590
Size: 338 156 87 613 282 4130
Fragments arranged by size:
4130 613 338 282 156 87
BbvCI CC'TCA_GC
Cuts at: 248 248
Size: 5606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1562 1596 1562
Size: 34 5572
BciVI GTATCCnnnnn_n'
Cuts at: 1913 3504 4265 5606 1913
Size: 1591 761 1341 1913
Fragments arranged by size:
1913 1591 1341 761
BclI T'GATC_A
Cuts at: 3126 3126
Size: 5606
BfrBI ATG'CAT
Cuts at: 1545 1545
Size: 5606
BglI GCCn_nnn'nGGC
Cuts at: 2135 2135
Size: 5606
BglII A'GATC_T
Cuts at: 1022 1640 3121 5158 1022
Size: 618 1481 2037 1470
Fragments arranged by size:
2037 1481 1470 618
BlpI GC'TnA_GC
Cuts at: 2718 2718
Size: 5606
Bme1580I G_kGCm'C
Cuts at: 966 1317 3231 3324 4380 966
Size: 351 1914 93 1056 2192
Fragments arranged by size:
2192 1914 1056 351 93
BmgBI CAC'GTC
Cuts at: 1131 1131
Size: 5606
BmrI ACTGGGnnnn_n'
Cuts at: 1034 2041 2818 2899 3234 5378 1034
Size: 1007 777 81 335 2144 1262
Fragments arranged by size:
2144 1262 1007 777 335 81
BmtI G_CTAG'C
Cuts at: 1402 1402
Size: 5606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3973 3973
Size: 5606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 734 1354 1582 2152 2299 456
Size: 278 620 228 570 147 3763
Fragments arranged by size:
3763 620 570 278 228 147
Bpu10I CC'TnA_GC
Cuts at: 248 248
Size: 5606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1198 2782 3661 4153 4451 814
Size: 384 1584 879 492 298 1969
Fragments arranged by size:
1969 1584 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 752 2272 5310 752
Size: 1520 3038 1048
Fragments arranged by size:
3038 1520 1048
BsaAI yAC'GTr
Cuts at: 2598 2959 2598
Size: 361 5245
BsaBI GATnn'nnATC
Cuts at: 6 620 3120 5157 6
Size: 614 2500 2037 455
Fragments arranged by size:
2500 2037 614 455
BsaHI Gr'CG_yC
Cuts at: 3037 3285 3987 5483 3037
Size: 248 702 1496 3160
Fragments arranged by size:
3160 1496 702 248
BsaWI w'CCGG_w
Cuts at: 257 2795 3316 3994 4268 4415 4826 257
Size: 2538 521 678 274 147 411 1037
Fragments arranged by size:
2538 1037 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 353 383 1175 1205 1327 1357 2188 2218 353
Size: 30 792 30 122 30 831 30 3741
Fragments arranged by size:
3741 831 792 122 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1351 1354 1351
Size: 3 5603
BseYI C'CCAG_C
Cuts at: 1383 3031 3789 4366 4862 1383
Size: 1648 758 577 496 2127
Fragments arranged by size:
2127 1648 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1519 1519
Size: 5606
BsiEI CG_ry'CG
Cuts at: 104 3148 3194 4402 104
Size: 3044 46 1208 1308
Fragments arranged by size:
3044 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1102 1151 2403 2620 3398 3588 4380 1102
Size: 49 1252 217 778 190 792 2328
Fragments arranged by size:
2328 1252 792 778 217 190 49
BsmI GAATG_Cn'
Cuts at: 1517 1517
Size: 5606
BsmBI CGTCTCn'nnnn_
Cuts at: 1178 1178
Size: 5606
Bsp1286I G_dGCh'C
Cuts at: 918 951 966 1102 1151 1317 2350 2403 2620
Size: 33 15 136 49 166 1033 53 217
Cuts at: 2620 3231 3324 3398 3588 3650 4380 918
Size: 611 93 74 190 62 730 2144
Fragments arranged by size:
2144 1033 730 611 217 190 166 136
93 74 62 53 49 33 15
BspEI T'CCGG_A
Cuts at: 257 4826 257
Size: 4569 1037
BspHI T'CATG_A
Cuts at: 4782 4782
Size: 5606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 1854 2656 2870 3172 3553 252
Size: 1602 802 214 302 381 2305
Fragments arranged by size:
2305 1602 802 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3898 3952 5436 5601 50
Size: 3848 54 1484 165 55
Fragments arranged by size:
3848 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 657 892 986 3518 657
Size: 235 94 2532 2745
Fragments arranged by size:
2745 2532 235 94
BsrFI r'CCGG_y
Cuts at: 743 3604 3785 3994 743
Size: 2861 181 209 2355
Fragments arranged by size:
2861 2355 209 181
BssSI C'ACGA_G
Cuts at: 3877 4235 3877
Size: 358 5248
BstAPI GCAn_nnn'nTGC
Cuts at: 1067 2983 1067
Size: 1916 3690
BstBI TT'CG_AA
Cuts at: 110 3967 110
Size: 3857 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2130 2130
Size: 5606
BstYI r'GATC_y
Cuts at: 1 971 1022 1640 2631 3098 3121 3456 3702
Size: 970 51 618 991 467 23 335 246
Cuts at: 3702 4714 4800 4812 5158 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 991 970 618 467 449 346 335
246 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 1871 1871
Size: 5606
BtgI C'CryG_G
Cuts at: 2064 2547 2064
Size: 483 5123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3556 3737 3900 3556
Size: 181 163 5262
Fragments arranged by size:
5262 181 163
BtsI GCAGTG_nn'
Cuts at: 159 465 1118 2849 159
Size: 306 653 1731 2916
Fragments arranged by size:
2916 1731 653 306
DraI TTT'AAA
Cuts at: 2274 2605 2274
Size: 331 5275
DrdI GACnn_nn'nnGTC
Cuts at: 3312 4170 3312
Size: 858 4748
EaeI y'GGCC_r
Cuts at: 1373 1943 3191 3365 3756 3783 5128 1373
Size: 570 1248 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 1248 570 391 174 27
EagI C'GGCC_G
Cuts at: 3191 3191
Size: 5606
EarI CTCTTCn'nnn_
Cuts at: 3629 3839 3629
Size: 210 5396
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3814 4124 4270 5416 3814
Size: 310 146 1146 4004
Fragments arranged by size:
4004 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2401 2618 2401
Size: 217 5389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 553 734 940 1354 1453 1582 1630 2035
Size: 97 181 206 414 99 129 48 405
Cuts at: 2035 2152 2299 3431 3863 4610 4878 456
Size: 117 147 1132 432 747 268 1184
Fragments arranged by size:
1184 1132 747 432 414 405 268 206
181 147 129 117 99 97 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1177 1177
Size: 5606
EcoO109I rG'GnC_Cy
Cuts at: 264 834 1557 2267 2310 2745 264
Size: 570 723 710 43 435 3125
Fragments arranged by size:
3125 723 710 570 435 43
EcoRI G'AATT_C
Cuts at: 2610 2610
Size: 5606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1678 3625 1678
Size: 1947 3659
FspI TGC'GCA
Cuts at: 3387 3387
Size: 5606
HaeII r_GCGC'y
Cuts at: 3040 3288 4310 5289 3040
Size: 248 1022 979 3357
Fragments arranged by size:
3357 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1819 1851 2363 2395 3448 3478 3480 3510 1819
Size: 32 512 32 1053 30 2 30 3915
Fragments arranged by size:
3915 1053 512 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2645 2645
Size: 5606
HindIII A'AGCT_T
Cuts at: 1321 1321
Size: 5606
KasI G'GCGC_C
Cuts at: 3036 3284 3036
Size: 248 5358
KpnI G_GTAC'C
Cuts at: 373 2626 373
Size: 2253 3353
MluI A'CGCG_T
Cuts at: 5039 5039
Size: 5606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 471 4277 4461 471
Size: 3806 184 1616
Fragments arranged by size:
3806 1616 184
MscI TGG'CCA
Cuts at: 1945 3367 1945
Size: 1422 4184
MslI CAynn'nnrTG
Cuts at: 662 1528 1864 3595 3722 662
Size: 866 336 1731 127 2546
Fragments arranged by size:
2546 1731 866 336 127
MspA1I CmG'CkG
Cuts at: 848 959 2141 2717 3031 3391 4404 4649 848
Size: 111 1182 576 314 360 1013 245 1805
Fragments arranged by size:
1805 1182 1013 576 360 314 245 111
NaeI GCC'GGC
Cuts at: 3787 3787
Size: 5606
NarI GG'CG_CC
Cuts at: 3037 3285 3037
Size: 248 5358
NcoI C'CATG_G
Cuts at: 2064 2547 2064
Size: 483 5123
NgoMIV G'CCGG_C
Cuts at: 3785 3785
Size: 5606
NheI G'CTAG_C
Cuts at: 1398 1398
Size: 5606
NsiI A_TGCA'T
Cuts at: 1547 1547
Size: 5606
NspI r_CATG'y
Cuts at: 1863 2659 3159 4066 1863
Size: 796 500 907 3403
Fragments arranged by size:
3403 907 796 500
PciI A'CATG_T
Cuts at: 4062 4062
Size: 5606
PflMI CCAn_nnn'nTGG
Cuts at: 1046 1560 2291 1046
Size: 514 731 4361
Fragments arranged by size:
4361 731 514
PfoI T'CCnGG_A
Cuts at: 647 647
Size: 5606
PmeI GTTT'AAAC
Cuts at: 2605 2605
Size: 5606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1327 1359 2188 2220 4745 4777 1327
Size: 32 829 32 2525 32 2156
Fragments arranged by size:
2525 2156 829 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 264 834 1557 2267 2310 264
Size: 570 723 710 43 3560
Fragments arranged by size:
3560 723 710 570 43
PshAI GACnn'nnGTC
Cuts at: 170 170
Size: 5606
PspXI vC'TCGA_Gb
Cuts at: 2172 2172
Size: 5606
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 419 451 419
Size: 32 5574
PstI C_TGCA'G
Cuts at: 2394 2653 3338 2394
Size: 259 685 4662
Fragments arranged by size:
4662 685 259
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5606
PvuII CAG'CTG
Cuts at: 848 959 2141 3031 3391 848
Size: 111 1182 890 360 3063
Fragments arranged by size:
3063 1182 890 360 111
RsrII CG'GwC_CG
Cuts at: 3801 3801
Size: 5606
SacI G_AGCT'C
Cuts at: 2403 2620 2403
Size: 217 5389
SalI G'TCGA_C
Cuts at: 2643 2643
Size: 5606
SapI GCTCTTCn'nnn_
Cuts at: 3629 3839 3629
Size: 210 5396
SbfI CC_TGCA'GG
Cuts at: 2653 2653
Size: 5606
ScaI AGT'ACT
Cuts at: 5123 5123
Size: 5606
SfcI C'TryA_G
Cuts at: 33 2249 2390 2649 3334 4327 4518 33
Size: 2216 141 259 685 993 191 1121
Fragments arranged by size:
2216 1121 993 685 259 191 141
SfoI GGC'GCC
Cuts at: 3038 3286 3038
Size: 248 5358
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5606
SmaI CCC'GGG
Cuts at: 2628 2628
Size: 5606
SmlI C'TyrA_G
Cuts at: 829 1213 2172 2761 3676 4168 4430 829
Size: 384 959 589 915 492 262 2005
Fragments arranged by size:
2005 959 915 589 492 384 262
SnaBI TAC'GTA
Cuts at: 2598 2598
Size: 5606
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5606
SphI G_CATG'C
Cuts at: 2659 3159 2659
Size: 500 5106
StuI AGG'CCT
Cuts at: 141 814 141
Size: 673 4933
StyI C'CwwG_G
Cuts at: 1352 2064 2351 2547 2740 3094 1352
Size: 712 287 196 193 354 3864
Fragments arranged by size:
3864 712 354 287 196 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1053 2173 2283 3189 3322 3781 3990 1053
Size: 1120 110 906 133 459 209 2669
Fragments arranged by size:
2669 1120 906 459 209 133 110
TatI w'GTAC_w
Cuts at: 5121 5121
Size: 5606
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1175 1207 2302 2334 5123 5155 1175
Size: 32 1095 32 2789 32 1626
Fragments arranged by size:
2789 1626 1095 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3403 3403
Size: 5606
XbaI T'CTAG_A
Cuts at: 2533 2637 2533
Size: 104 5502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 845 1043 1401 3101 845
Size: 198 358 1700 3350
Fragments arranged by size:
3350 1700 358 198
XhoI C'TCGA_G
Cuts at: 2172 2172
Size: 5606
XmaI C'CCGG_G
Cuts at: 2626 2626
Size: 5606
XmnI GAAnn'nnTTC
Cuts at: 1517 1517
Size: 5606
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI PacI PasI PmlI PsiI PspOMI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI ZraI