Restriction map for
pF1KSDA0217
(Circular) (Six-base) MAPSORT of: pF1KSDA0217.seq Check: 1048 from: 1 to: 5351
pF1KSDA0217 5351 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2025 2025
Size: 5351
AatII G_ACGT'C
Cuts at: 1144 1144
Size: 5351
AccI GT'mk_AC
Cuts at: 2389 2389
Size: 5351
Acc65I G'GTAC_C
Cuts at: 267 2367 267
Size: 2100 3251
AclI AA'CG_TT
Cuts at: 5191 5191
Size: 5351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 3176 3608 4355 4623 1511
Size: 1665 432 747 268 2239
Fragments arranged by size:
2239 1665 747 432 268
AfeI AGC'GCT
Cuts at: 198 1132 198
Size: 934 4417
AflIII A'CryG_T
Cuts at: 653 2703 3807 4784 653
Size: 2050 1104 977 1220
Fragments arranged by size:
2050 1220 1104 977
AgeI A'CCGG_T
Cuts at: 3739 3739
Size: 5351
AleI CACnn'nnGTG
Cuts at: 703 742 703
Size: 39 5312
AlwNI CAG_nnn'CTG
Cuts at: 973 2656 4223 973
Size: 1683 1567 2101
Fragments arranged by size:
2101 1683 1567
ApaLI G'TGCA_C
Cuts at: 4121 4121
Size: 5351
ApoI r'AATT_y
Cuts at: 619 913 928 1614 2355 4704 4960 619
Size: 294 15 686 741 2349 256 1010
Fragments arranged by size:
2349 1010 741 686 294 256 15
AseI AT'TA_AT
Cuts at: 20 1459 1463 20
Size: 1439 4 3908
Fragments arranged by size:
3908 1439 4
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
AvaI C'yCGr_G
Cuts at: 593 2371 593
Size: 1778 3573
BamHI G'GATC_C
Cuts at: 2376 2376
Size: 5351
BanI G'GyrC_C
Cuts at: 267 1881 2367 2781 3029 3064 267
Size: 1614 486 414 248 35 2554
Fragments arranged by size:
2554 1614 486 414 248 35
BanII G_rGCy'C
Cuts at: 321 1877 2192 2213 2241 2365 3395 321
Size: 1556 315 21 28 124 1030 2277
Fragments arranged by size:
2277 1556 1030 315 124 28 21
BbeI G_GCGC'C
Cuts at: 2785 3033 2785
Size: 248 5103
BbsI GAAGACnn'nnnn_
Cuts at: 593 1765 593
Size: 1172 4179
BciVI GTATCCnnnnn_n'
Cuts at: 1352 3249 4010 5351 1352
Size: 1897 761 1341 1352
Fragments arranged by size:
1897 1352 1341 761
BclI T'GATC_A
Cuts at: 135 1199 2871 135
Size: 1064 1672 2615
Fragments arranged by size:
2615 1672 1064
BglII A'GATC_T
Cuts at: 769 2866 4903 769
Size: 2097 2037 1217
Fragments arranged by size:
2097 2037 1217
BlpI GC'TnA_GC
Cuts at: 733 2463 733
Size: 1730 3621
Bme1580I G_kGCm'C
Cuts at: 2976 3069 4125 2976
Size: 93 1056 4202
Fragments arranged by size:
4202 1056 93
BmgBI CAC'GTC
Cuts at: 1110 1214 1110
Size: 104 5247
BmrI ACTGGGnnnn_n'
Cuts at: 2563 2644 2979 5123 2563
Size: 81 335 2144 2791
Fragments arranged by size:
2791 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 5351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1280 1760 1851 2255 1280
Size: 480 91 404 4376
Fragments arranged by size:
4376 480 404 91
Bpu10I CC'TnA_GC
Cuts at: 146 146
Size: 5351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 2527 3406 3898 4196 2206
Size: 321 879 492 298 3361
Fragments arranged by size:
3361 879 492 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 1869 2240 5055 1869
Size: 371 2815 2165
Fragments arranged by size:
2815 2165 371
BsaAI yAC'GTr
Cuts at: 2343 2704 2343
Size: 361 4990
BsaBI GATnn'nnATC
Cuts at: 6 2865 4902 6
Size: 2859 2037 455
Fragments arranged by size:
2859 2037 455
BsaHI Gr'CG_yC
Cuts at: 1141 1315 2782 3030 3732 5228 1141
Size: 174 1467 248 702 1496 1264
Fragments arranged by size:
1496 1467 1264 702 248 174
BsaWI w'CCGG_w
Cuts at: 1472 1628 2540 3061 3739 4013 4160 4571 1472
Size: 156 912 521 678 274 147 411 2252
Fragments arranged by size:
2252 912 678 521 411 274 156 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 794 824 1633 1656 1663 1686 794
Size: 30 809 23 7 23 4459
Fragments arranged by size:
4459 809 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 1310 1337 1657 2324 391
Size: 919 27 320 667 3418
Fragments arranged by size:
3418 919 667 320 27
BseYI C'CCAG_C
Cuts at: 486 1124 1172 1549 1710 1929 2068 2214 2776
Size: 638 48 377 161 219 139 146 562
Cuts at: 2776 3534 4111 4607 486
Size: 758 577 496 1230
Fragments arranged by size:
1230 758 638 577 562 496 377 219
161 146 139 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1850 2053 1418
Size: 432 203 4716
Fragments arranged by size:
4716 432 203
BsiEI CG_ry'CG
Cuts at: 104 2018 2260 2893 2939 4147 104
Size: 1914 242 633 46 1208 1308
Fragments arranged by size:
1914 1308 1208 633 242 46
BsiHKAI G_wGCw'C
Cuts at: 2320 2365 3143 3333 4125 2320
Size: 45 778 190 792 3546
Fragments arranged by size:
3546 792 778 190 45
BsiWI C'GTAC_G
Cuts at: 1910 1910
Size: 5351
BsmI GAATG_Cn'
Cuts at: 459 459
Size: 5351
BsmBI CGTCTCn'nnnn_
Cuts at: 1298 1322 2305 1298
Size: 24 983 4344
Fragments arranged by size:
4344 983 24
Bsp1286I G_dGCh'C
Cuts at: 321 1877 2192 2213 2241 2320 2365 2976 3069
Size: 1556 315 21 28 79 45 611 93
Cuts at: 3069 3143 3333 3395 4125 321
Size: 74 190 62 730 1547
Fragments arranged by size:
1556 1547 730 611 315 190 93 79
74 62 45 28 21
BspEI T'CCGG_A
Cuts at: 1472 4571 1472
Size: 3099 2252
BspHI T'CATG_A
Cuts at: 4527 4527
Size: 5351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1486 2025 2401 2615 2917 3298 1486
Size: 539 376 214 302 381 3539
Fragments arranged by size:
3539 539 381 376 302 214
BsrBI CCG'CTC
Cuts at: 50 3643 3697 5181 5346 50
Size: 3593 54 1484 165 55
Fragments arranged by size:
3593 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 460 2119 3263 460
Size: 1659 1144 2548
Fragments arranged by size:
2548 1659 1144
BsrFI r'CCGG_y
Cuts at: 1747 1884 2259 2270 3349 3530 3739 1747
Size: 137 375 11 1079 181 209 3359
Fragments arranged by size:
3359 1079 375 209 181 137 11
BsrGI T'GTAC_A
Cuts at: 1163 1190 1163
Size: 27 5324
BssSI C'ACGA_G
Cuts at: 3622 3980 3622
Size: 358 4993
BstAPI GCAn_nnn'nTGC
Cuts at: 346 2728 346
Size: 2382 2969
BstBI TT'CG_AA
Cuts at: 110 3712 110
Size: 3602 1749
BstEII G'GTnAC_C
Cuts at: 436 436
Size: 5351
BstYI r'GATC_y
Cuts at: 1 769 2376 2843 2866 3201 3447 4459 4545
Size: 768 1607 467 23 335 246 1012 86
Cuts at: 4545 4557 4903 1
Size: 12 346 449
Fragments arranged by size:
1607 1012 768 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 402 869 2051 2288 402
Size: 467 1182 237 3465
Fragments arranged by size:
3465 1182 467 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3301 3482 3645 3301
Size: 181 163 5007
Fragments arranged by size:
5007 181 163
BtsI GCAGTG_nn'
Cuts at: 351 1445 2009 2594 351
Size: 1094 564 585 3108
Fragments arranged by size:
3108 1094 585 564
ClaI AT'CG_AT
Cuts at: 1455 1455
Size: 5351
DraI TTT'AAA
Cuts at: 894 2350 894
Size: 1456 3895
DraIII CAC_nnn'GTG
Cuts at: 1986 1986
Size: 5351
DrdI GACnn_nn'nnGTC
Cuts at: 3057 3915 3057
Size: 858 4493
EaeI y'GGCC_r
Cuts at: 387 2257 2285 2936 3110 3501 3528 4873 387
Size: 1870 28 651 174 391 27 1345 865
Fragments arranged by size:
1870 1345 865 651 391 174 28 27
EagI C'GGCC_G
Cuts at: 2257 2936 2257
Size: 679 4672
EarI CTCTTCn'nnn_
Cuts at: 3374 3584 3374
Size: 210 5141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 1992 3559 3869 4015 5161 1975
Size: 17 1567 310 146 1146 2165
Fragments arranged by size:
2165 1567 1146 310 146 17
EcoICRI GAG'CTC
Cuts at: 2363 2363
Size: 5351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1280 1511 1760 1851 2255 3176 3608 4355 4623
Size: 231 249 91 404 921 432 747 268
Cuts at: 4623 1280
Size: 2008
Fragments arranged by size:
2008 921 747 432 404 268 249 231
91
EcoNI CCTnn'n_nnAGG
Cuts at: 1595 1595
Size: 5351
EcoO109I rG'GnC_Cy
Cuts at: 514 1408 1820 2490 514
Size: 894 412 670 3375
Fragments arranged by size:
3375 894 670 412
EcoRI G'AATT_C
Cuts at: 619 1614 2355 619
Size: 995 741 3615
Fragments arranged by size:
3615 995 741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1635 3370 1635
Size: 1735 3616
FseI GG_CCGG'CC
Cuts at: 2274 2274
Size: 5351
FspI TGC'GCA
Cuts at: 3132 3132
Size: 5351
HaeII r_GCGC'y
Cuts at: 200 1134 2785 3033 4055 5034 200
Size: 934 1651 248 1022 979 517
Fragments arranged by size:
1651 1022 979 934 517 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 198 230 995 1027 1656 1688 3193
Size: 32 46 32 765 32 629 32 1505
Cuts at: 3193 3223 3225 3255 120
Size: 30 2 30 2216
Fragments arranged by size:
2216 1505 765 629 46 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2390 2390
Size: 5351
HindIII A'AGCT_T
Cuts at: 1701 1701
Size: 5351
KasI G'GCGC_C
Cuts at: 2781 3029 2781
Size: 248 5103
KpnI G_GTAC'C
Cuts at: 271 2371 271
Size: 2100 3251
MluI A'CGCG_T
Cuts at: 4784 4784
Size: 5351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 1699 4022 4206 461
Size: 1238 2323 184 1606
Fragments arranged by size:
2323 1606 1238 184
MscI TGG'CCA
Cuts at: 389 3112 389
Size: 2723 2628
MslI CAynn'nnrTG
Cuts at: 452 703 742 3340 3467 452
Size: 251 39 2598 127 2336
Fragments arranged by size:
2598 2336 251 127 39
MspA1I CmG'CkG
Cuts at: 2462 2776 3136 4149 4394 2462
Size: 314 360 1013 245 3419
Fragments arranged by size:
3419 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1749 1886 2261 2272 3532 1749
Size: 137 375 11 1260 3568
Fragments arranged by size:
3568 1260 375 137 11
NarI GG'CG_CC
Cuts at: 2782 3030 2782
Size: 248 5103
NcoI C'CATG_G
Cuts at: 2288 2288
Size: 5351
NdeI CA'TA_TG
Cuts at: 1533 1533
Size: 5351
NgoMIV G'CCGG_C
Cuts at: 1747 1884 2259 2270 3530 1747
Size: 137 375 11 1260 3568
Fragments arranged by size:
3568 1260 375 137 11
NspI r_CATG'y
Cuts at: 657 1387 2404 2904 3811 657
Size: 730 1017 500 907 2197
Fragments arranged by size:
2197 1017 907 730 500
PacI TTA_AT'TAA
Cuts at: 1463 1463
Size: 5351
PciI A'CATG_T
Cuts at: 653 3807 653
Size: 3154 2197
PflMI CCAn_nnn'nTGG
Cuts at: 1090 1270 1738 1986 1090
Size: 180 468 248 4455
Fragments arranged by size:
4455 468 248 180
PfoI T'CCnGG_A
Cuts at: 179 179
Size: 5351
PmeI GTTT'AAAC
Cuts at: 2350 2350
Size: 5351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 5319
PpuMI rG'GwC_Cy
Cuts at: 514 1408 1820 514
Size: 894 412 4045
Fragments arranged by size:
4045 894 412
PsiI TTA'TAA
Cuts at: 919 1279 919
Size: 360 4991
PstI C_TGCA'G
Cuts at: 1452 2398 3083 1452
Size: 946 685 3720
Fragments arranged by size:
3720 946 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5351
PvuII CAG'CTG
Cuts at: 2776 3136 2776
Size: 360 4991
RsrII CG'GwC_CG
Cuts at: 3546 3546
Size: 5351
SacI G_AGCT'C
Cuts at: 2365 2365
Size: 5351
SalI G'TCGA_C
Cuts at: 2388 2388
Size: 5351
SanDI GG'GwC_CC
Cuts at: 514 514
Size: 5351
SapI GCTCTTCn'nnn_
Cuts at: 3374 3584 3374
Size: 210 5141
SbfI CC_TGCA'GG
Cuts at: 2398 2398
Size: 5351
ScaI AGT'ACT
Cuts at: 602 4868 602
Size: 4266 1085
SfcI C'TryA_G
Cuts at: 33 1448 1856 2394 3079 4072 4263 33
Size: 1415 408 538 685 993 191 1121
Fragments arranged by size:
1415 1121 993 685 538 408 191
SfoI GGC'GCC
Cuts at: 2783 3031 2783
Size: 248 5103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
SmaI CCC'GGG
Cuts at: 2373 2373
Size: 5351
SmlI C'TyrA_G
Cuts at: 2221 2506 3421 3913 4175 2221
Size: 285 915 492 262 3397
Fragments arranged by size:
3397 915 492 285 262
SnaBI TAC'GTA
Cuts at: 2343 2343
Size: 5351
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5351
SphI G_CATG'C
Cuts at: 1387 2404 2904 1387
Size: 1017 500 3834
Fragments arranged by size:
3834 1017 500
SspI AAT'ATT
Cuts at: 842 1436 842
Size: 594 4757
StyI C'CwwG_G
Cuts at: 1247 1952 2288 2485 2839 1247
Size: 705 336 197 354 3759
Fragments arranged by size:
3759 705 354 336 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2934 3067 3526 3735 2934
Size: 133 459 209 4550
Fragments arranged by size:
4550 459 209 133
TatI w'GTAC_w
Cuts at: 600 1163 1190 4866 600
Size: 563 27 3676 1085
Fragments arranged by size:
3676 1085 563 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1812 1844 4868 4900 1812
Size: 32 3024 32 2263
Fragments arranged by size:
3024 2263 32 32
Tth111I GACn'n_nGTC
Cuts at: 1215 1668 3148 1215
Size: 453 1480 3418
Fragments arranged by size:
3418 1480 453
XbaI T'CTAG_A
Cuts at: 2382 2382
Size: 5351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 400 2846 400
Size: 2446 2905
XmaI C'CCGG_G
Cuts at: 2371 2371
Size: 5351
ZraI GAC'GTC
Cuts at: 1142 1142
Size: 5351
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PacI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BssHII BstXI BstZ17I
Bsu36I CspCI EcoRV FspAI HpaI MfeI NheI NotI
NruI NsiI PasI PmlI PshAI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI StuI SwaI XhoI
XmnI