Restriction map for
pF1KSDA0218
(Circular) (Six-base) MAPSORT of: pF1KSDA0218.seq Check: 2194 from: 1 to: 5420
pF1KSDA0218 5420 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2458 2458
Size: 5420
Acc65I G'GTAC_C
Cuts at: 2436 2436
Size: 5420
AclI AA'CG_TT
Cuts at: 5260 5260
Size: 5420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 611 709 1172 2149 3245 3677 4424 4692
Size: 168 98 463 977 1096 432 747 268
Cuts at: 4692 443
Size: 1171
Fragments arranged by size:
1171 1096 977 747 463 432 268 168
98
AflII C'TTAA_G
Cuts at: 535 1872 535
Size: 1337 4083
AflIII A'CryG_T
Cuts at: 2144 2772 3876 4853 2144
Size: 628 1104 977 2711
Fragments arranged by size:
2711 1104 977 628
AgeI A'CCGG_T
Cuts at: 3808 3808
Size: 5420
AleI CACnn'nnGTG
Cuts at: 2164 2164
Size: 5420
AlwNI CAG_nnn'CTG
Cuts at: 856 2725 4292 856
Size: 1869 1567 1984
Fragments arranged by size:
1984 1869 1567
ApaI G_GGCC'C
Cuts at: 272 397 272
Size: 125 5295
ApaLI G'TGCA_C
Cuts at: 1929 4190 1929
Size: 2261 3159
ApoI r'AATT_y
Cuts at: 580 1455 1685 1711 2424 4773 5029 580
Size: 875 230 26 713 2349 256 971
Fragments arranged by size:
2349 971 875 713 256 230 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5420
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
AvaI C'yCGr_G
Cuts at: 443 749 2195 2301 2440 443
Size: 306 1446 106 139 3423
Fragments arranged by size:
3423 1446 306 139 106
BamHI G'GATC_C
Cuts at: 466 2445 466
Size: 1979 3441
BanI G'GyrC_C
Cuts at: 1877 2436 2850 3098 3133 1877
Size: 559 414 248 35 4164
Fragments arranged by size:
4164 559 414 248 35
BanII G_rGCy'C
Cuts at: 212 272 343 397 673 986 2123 2434 3464
Size: 60 71 54 276 313 1137 311 1030
Cuts at: 3464 212
Size: 2168
Fragments arranged by size:
2168 1137 1030 313 311 276 71 60
54
BbeI G_GCGC'C
Cuts at: 2854 3102 2854
Size: 248 5172
BbsI GAAGACnn'nnnn_
Cuts at: 449 449
Size: 5420
BbvCI CC'TCA_GC
Cuts at: 596 596
Size: 5420
BciVI GTATCCnnnnn_n'
Cuts at: 3318 4079 5420 3318
Size: 761 1341 3318
Fragments arranged by size:
3318 1341 761
BclI T'GATC_A
Cuts at: 2940 2940
Size: 5420
BglI GCCn_nnn'nGGC
Cuts at: 751 2191 751
Size: 1440 3980
BglII A'GATC_T
Cuts at: 1139 2034 2935 4972 1139
Size: 895 901 2037 1587
Fragments arranged by size:
2037 1587 901 895
BlpI GC'TnA_GC
Cuts at: 2532 2532
Size: 5420
Bme1580I G_kGCm'C
Cuts at: 272 397 1933 2066 3045 3138 4194 272
Size: 125 1536 133 979 93 1056 1498
Fragments arranged by size:
1536 1498 1056 979 133 125 93
BmgBI CAC'GTC
Cuts at: 170 170
Size: 5420
BmrI ACTGGGnnnn_n'
Cuts at: 968 2632 2713 3048 5192 968
Size: 1664 81 335 2144 1196
Fragments arranged by size:
2144 1664 1196 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1122 1158 3787 1122
Size: 36 2629 2755
Fragments arranged by size:
2755 2629 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 213 284 1219 1401 1461 1606 2242 180
Size: 33 71 935 182 60 145 636 3358
Fragments arranged by size:
3358 935 636 182 145 71 60 33
Bpu10I CC'TnA_GC
Cuts at: 596 596
Size: 5420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 947 2226 2596 3475 3967 4265 947
Size: 1279 370 879 492 298 2102
Fragments arranged by size:
2102 1279 879 492 370 298
BsaI GGTCTCn'nnnn_
Cuts at: 656 5124 656
Size: 4468 952
BsaAI yAC'GTr
Cuts at: 2412 2773 2412
Size: 361 5059
BsaBI GATnn'nnATC
Cuts at: 6 2033 2934 4971 6
Size: 2027 901 2037 455
Fragments arranged by size:
2037 2027 901 455
BsaHI Gr'CG_yC
Cuts at: 130 2851 3099 3801 5297 130
Size: 2721 248 702 1496 253
Fragments arranged by size:
2721 1496 702 253 248
BsaWI w'CCGG_w
Cuts at: 2609 3130 3808 4082 4229 4640 2609
Size: 521 678 274 147 411 3389
Fragments arranged by size:
3389 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 382 655 685 1681 1711 1762 1792 2232
Size: 30 273 30 996 30 51 30 440
Cuts at: 2232 2262 352
Size: 30 3510
Fragments arranged by size:
3510 996 440 273 51 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 354 357 360 532 916 939 994 1150 1229
Size: 3 3 172 384 23 55 156 79
Cuts at: 1229 1540 1615 1786 2169 354
Size: 311 75 171 383 3605
Fragments arranged by size:
3605 384 383 311 172 171 156 79
75 55 23 3 3
BseYI C'CCAG_C
Cuts at: 271 778 2845 3603 4180 4676 271
Size: 507 2067 758 577 496 1015
Fragments arranged by size:
2067 1015 758 577 507 496
BsiEI CG_ry'CG
Cuts at: 104 2962 3008 4216 104
Size: 2858 46 1208 1308
Fragments arranged by size:
2858 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1103 1933 2434 3212 3402 4194 1103
Size: 830 501 778 190 792 2329
Fragments arranged by size:
2329 830 792 778 501 190
Bsp1286I G_dGCh'C
Cuts at: 212 272 343 397 673 986 1103 1933 2066
Size: 60 71 54 276 313 117 830 133
Cuts at: 2066 2123 2434 3045 3138 3212 3402 3464 4194
Size: 57 311 611 93 74 190 62 730
Cuts at: 4194 212
Size: 1438
Fragments arranged by size:
1438 830 730 611 313 311 276 190
133 117 93 74 71 62 60 57
54
BspEI T'CCGG_A
Cuts at: 4640 4640
Size: 5420
BspHI T'CATG_A
Cuts at: 2048 4596 2048
Size: 2548 2872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2470 2684 2986 3367 2470
Size: 214 302 381 4523
Fragments arranged by size:
4523 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 3712 3766 5250 5415 50
Size: 88 3574 54 1484 165 55
Fragments arranged by size:
3574 1484 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 2088 3332 2088
Size: 1244 4176
BsrFI r'CCGG_y
Cuts at: 233 2098 3418 3599 3808 233
Size: 1865 1320 181 209 1845
Fragments arranged by size:
1865 1845 1320 209 181
BsrGI T'GTAC_A
Cuts at: 1247 1247
Size: 5420
BssSI C'ACGA_G
Cuts at: 3691 4049 3691
Size: 358 5062
BstAPI GCAn_nnn'nTGC
Cuts at: 2797 2797
Size: 5420
BstBI TT'CG_AA
Cuts at: 110 3781 110
Size: 3671 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1425 1425
Size: 5420
BstYI r'GATC_y
Cuts at: 1 466 1139 1788 2034 2078 2213 2445 2912
Size: 465 673 649 246 44 135 232 467
Cuts at: 2912 2935 3270 3516 4528 4614 4626 4972 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 673 649 467 465 449 346 335
246 246 232 135 86 44 23 12
Bsu36I CC'TnA_GG
Cuts at: 1706 1883 1706
Size: 177 5243
BtgI C'CryG_G
Cuts at: 125 783 125
Size: 658 4762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3370 3551 3714 3370
Size: 181 163 5076
Fragments arranged by size:
5076 181 163
BtsI GCAGTG_nn'
Cuts at: 854 2013 2171 2663 854
Size: 1159 158 492 3611
Fragments arranged by size:
3611 1159 492 158
ClaI AT'CG_AT
Cuts at: 718 718
Size: 5420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 371 406 371
Size: 35 5385
DraI TTT'AAA
Cuts at: 2419 2419
Size: 5420
DrdI GACnn_nn'nnGTC
Cuts at: 3126 3984 3126
Size: 858 4562
EaeI y'GGCC_r
Cuts at: 235 3005 3179 3570 3597 4942 235
Size: 2770 174 391 27 1345 713
Fragments arranged by size:
2770 1345 713 391 174 27
EagI C'GGCC_G
Cuts at: 3005 3005
Size: 5420
EarI CTCTTCn'nnn_
Cuts at: 640 1113 1348 3443 3653 640
Size: 473 235 2095 210 2407
Fragments arranged by size:
2407 2095 473 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3628 3938 4084 5230 3628
Size: 310 146 1146 3818
Fragments arranged by size:
3818 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2432 2432
Size: 5420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 213 284 443 611 709 1172 1219 1401
Size: 33 71 159 168 98 463 47 182
Cuts at: 1401 1461 1606 2149 2242 3245 3677 4424 4692
Size: 60 145 543 93 1003 432 747 268
Cuts at: 4692 180
Size: 908
Fragments arranged by size:
1003 908 747 543 463 432 268 182
168 159 145 98 93 71 60 47
33
EcoNI CCTnn'n_nnAGG
Cuts at: 1547 1600 1768 1547
Size: 53 168 5199
Fragments arranged by size:
5199 168 53
EcoO109I rG'GnC_Cy
Cuts at: 268 394 1664 1802 2559 268
Size: 126 1270 138 757 3129
Fragments arranged by size:
3129 1270 757 138 126
EcoRI G'AATT_C
Cuts at: 1455 2424 1455
Size: 969 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2019 3439 2019
Size: 1420 4000
FspI TGC'GCA
Cuts at: 3201 3201
Size: 5420
HaeII r_GCGC'y
Cuts at: 288 2854 3102 4124 5103 288
Size: 2566 248 1022 979 605
Fragments arranged by size:
2566 1022 979 605 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 1003 1035 1036 1068 3262 3292 3294
Size: 32 316 32 1 32 2194 30 2
Cuts at: 3294 3324 655
Size: 30 2751
Fragments arranged by size:
2751 2194 316 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 2459 2459
Size: 5420
KasI G'GCGC_C
Cuts at: 2850 3098 2850
Size: 248 5172
KpnI G_GTAC'C
Cuts at: 2440 2440
Size: 5420
MfeI C'AATT_G
Cuts at: 614 614
Size: 5420
MluI A'CGCG_T
Cuts at: 4853 4853
Size: 5420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 152 4091 4275 152
Size: 3939 184 1297
Fragments arranged by size:
3939 1297 184
MscI TGG'CCA
Cuts at: 3181 3181
Size: 5420
MslI CAynn'nnrTG
Cuts at: 2149 2164 3409 3536 2149
Size: 15 1245 127 4033
Fragments arranged by size:
4033 1245 127 15
MspA1I CmG'CkG
Cuts at: 197 589 1974 1980 2531 2845 3205 4218 4463
Size: 392 1385 6 551 314 360 1013 245
Cuts at: 4463 197
Size: 1154
Fragments arranged by size:
1385 1154 1013 551 392 360 314 245
6
NaeI GCC'GGC
Cuts at: 235 3601 235
Size: 3366 2054
NarI GG'CG_CC
Cuts at: 2851 3099 2851
Size: 248 5172
NcoI C'CATG_G
Cuts at: 125 783 125
Size: 658 4762
NgoMIV G'CCGG_C
Cuts at: 233 3599 233
Size: 3366 2054
NspI r_CATG'y
Cuts at: 1638 2148 2473 2973 3880 1638
Size: 510 325 500 907 3178
Fragments arranged by size:
3178 907 510 500 325
PciI A'CATG_T
Cuts at: 2144 3876 2144
Size: 1732 3688
PflMI CCAn_nnn'nTGG
Cuts at: 1280 1280
Size: 5420
PfoI T'CCnGG_A
Cuts at: 203 203
Size: 5420
PmeI GTTT'AAAC
Cuts at: 2419 2419
Size: 5420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 4559
Size: 32 5388
PpuMI rG'GwC_Cy
Cuts at: 1664 1664
Size: 5420
PspOMI G'GGCC_C
Cuts at: 268 393 268
Size: 125 5295
PstI C_TGCA'G
Cuts at: 532 828 1979 2467 3152 532
Size: 296 1151 488 685 2800
Fragments arranged by size:
2800 1151 685 488 296
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5420
PvuII CAG'CTG
Cuts at: 197 589 1974 1980 2845 3205 197
Size: 392 1385 6 865 360 2412
Fragments arranged by size:
2412 1385 865 392 360 6
RsrII CG'GwC_CG
Cuts at: 2322 3615 2322
Size: 1293 4127
SacI G_AGCT'C
Cuts at: 2434 2434
Size: 5420
SalI G'TCGA_C
Cuts at: 2457 2457
Size: 5420
SapI GCTCTTCn'nnn_
Cuts at: 1348 3443 3653 1348
Size: 2095 210 3115
Fragments arranged by size:
3115 2095 210
SbfI CC_TGCA'GG
Cuts at: 2467 2467
Size: 5420
ScaI AGT'ACT
Cuts at: 2134 4937 2134
Size: 2803 2617
SfcI C'TryA_G
Cuts at: 33 528 824 1394 1836 1975 2400 2463 3148
Size: 495 296 570 442 139 425 63 685
Cuts at: 3148 4141 4332 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 570 495 442 425 296
191 139 63
SfoI GGC'GCC
Cuts at: 2852 3100 2852
Size: 248 5172
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
SmaI CCC'GGG
Cuts at: 2197 2303 2442 2197
Size: 106 139 5175
Fragments arranged by size:
5175 139 106
SmlI C'TyrA_G
Cuts at: 535 962 1872 2205 2575 3490 3982 4244 535
Size: 427 910 333 370 915 492 262 1711
Fragments arranged by size:
1711 915 910 492 427 370 333 262
SnaBI TAC'GTA
Cuts at: 2412 2412
Size: 5420
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5420
SphI G_CATG'C
Cuts at: 2473 2973 2473
Size: 500 4920
StuI AGG'CCT
Cuts at: 1432 1432
Size: 5420
StyI C'CwwG_G
Cuts at: 125 783 987 1258 1659 2003 2366 2554 2908
Size: 658 204 271 401 344 363 188 354
Cuts at: 2908 125
Size: 2637
Fragments arranged by size:
2637 658 401 363 354 344 271 204
188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1010 3003 3136 3595 3804 1010
Size: 1993 133 459 209 2626
Fragments arranged by size:
2626 1993 459 209 133
TatI w'GTAC_w
Cuts at: 1247 2132 4935 1247
Size: 885 2803 1732
Fragments arranged by size:
2803 1732 885
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4937 4969 4937
Size: 32 5388
Tth111I GACn'n_nGTC
Cuts at: 1225 3217 1225
Size: 1992 3428
XbaI T'CTAG_A
Cuts at: 513 2451 513
Size: 1938 3482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 2148 2915 492
Size: 1656 767 2997
Fragments arranged by size:
2997 1656 767
XmaI C'CCGG_G
Cuts at: 2195 2301 2440 2195
Size: 106 139 5175
Fragments arranged by size:
5175 139 106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI