Restriction map for
pF1KSDA0221
(Circular) (Six-base) MAPSORT of: pF1KSDA0221.seq Check: 422 from: 1 to: 6524
pF1KSDA0221 6524 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2828 2828
Size: 6524
AatII G_ACGT'C
Cuts at: 1663 1663
Size: 6524
AccI GT'mk_AC
Cuts at: 1408 1676 3562 1408
Size: 268 1886 4370
Fragments arranged by size:
4370 1886 268
Acc65I G'GTAC_C
Cuts at: 1940 3540 1940
Size: 1600 4924
AclI AA'CG_TT
Cuts at: 1444 6364 1444
Size: 4920 1604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 2521 4349 4781 5528 5796 1105
Size: 1416 1828 432 747 268 1833
Fragments arranged by size:
1833 1828 1416 747 432 268
AfeI AGC'GCT
Cuts at: 1543 2557 1543
Size: 1014 5510
AflIII A'CryG_T
Cuts at: 2005 3876 4980 5957 2005
Size: 1871 1104 977 2572
Fragments arranged by size:
2572 1871 1104 977
AgeI A'CCGG_T
Cuts at: 4912 4912
Size: 6524
AleI CACnn'nnGTG
Cuts at: 2008 2008
Size: 6524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 779 779
Size: 6524
AlwNI CAG_nnn'CTG
Cuts at: 500 819 1887 1896 3829 5396 500
Size: 319 1068 9 1933 1567 1628
Fragments arranged by size:
1933 1628 1567 1068 319 9
ApaI G_GGCC'C
Cuts at: 149 365 650 1049 1636 2161 149
Size: 216 285 399 587 525 4512
Fragments arranged by size:
4512 587 525 399 285 216
ApaLI G'TGCA_C
Cuts at: 1358 5294 1358
Size: 3936 2588
ApoI r'AATT_y
Cuts at: 3528 5877 6133 3528
Size: 2349 256 3919
Fragments arranged by size:
3919 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6524
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
AvaI C'yCGr_G
Cuts at: 285 309 2025 2113 2436 2931 2969 3544 285
Size: 24 1716 88 323 495 38 575 3265
Fragments arranged by size:
3265 1716 575 495 323 88 38 24
BamHI G'GATC_C
Cuts at: 3549 3549
Size: 6524
BanI G'GyrC_C
Cuts at: 199 1940 2014 2440 2509 3184 3313 3540 3954
Size: 1741 74 426 69 675 129 227 414
Cuts at: 3954 4202 4237 199
Size: 248 35 2486
Fragments arranged by size:
2486 1741 675 426 414 248 227 129
74 69 35
BanII G_rGCy'C
Cuts at: 149 217 331 365 650 1049 1636 2161 2317
Size: 68 114 34 285 399 587 525 156
Cuts at: 2317 2940 3538 4568 149
Size: 623 598 1030 2105
Fragments arranged by size:
2105 1030 623 598 587 525 399 285
156 114 68 34
BbeI G_GCGC'C
Cuts at: 203 2018 2513 3317 3958 4206 203
Size: 1815 495 804 641 248 2521
Fragments arranged by size:
2521 1815 804 641 495 248
BbsI GAAGACnn'nnnn_
Cuts at: 461 690 1603 1657 461
Size: 229 913 54 5328
Fragments arranged by size:
5328 913 229 54
BciVI GTATCCnnnnn_n'
Cuts at: 4422 5183 6524 4422
Size: 761 1341 4422
Fragments arranged by size:
4422 1341 761
BclI T'GATC_A
Cuts at: 3062 4044 3062
Size: 982 5542
BglI GCCn_nnn'nGGC
Cuts at: 281 314 1640 2156 2974 3368 281
Size: 33 1326 516 818 394 3437
Fragments arranged by size:
3437 1326 818 516 394 33
BglII A'GATC_T
Cuts at: 4039 6076 4039
Size: 2037 4487
BlpI GC'TnA_GC
Cuts at: 3636 3636
Size: 6524
Bme1580I G_kGCm'C
Cuts at: 149 365 650 888 1049 1362 1636 2161 2443
Size: 216 285 238 161 313 274 525 282
Cuts at: 2443 4149 4242 5298 149
Size: 1706 93 1056 1375
Fragments arranged by size:
1706 1375 1056 525 313 285 282 274
238 216 161 93
BmgBI CAC'GTC
Cuts at: 568 1296 3435 568
Size: 728 2139 3657
Fragments arranged by size:
3657 2139 728
BmrI ACTGGGnnnn_n'
Cuts at: 804 1919 2155 3501 3736 3817 4152 6296 804
Size: 1115 236 1346 235 81 335 2144 1032
Fragments arranged by size:
2144 1346 1115 1032 335 236 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4891 4891
Size: 6524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 940 976 985 1081 2305 688
Size: 252 36 9 96 1224 4907
Fragments arranged by size:
4907 1224 252 96 36 9
Bpu10I CC'TnA_GC
Cuts at: 1874 1874
Size: 6524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1085 3700 4579 5071 5369 766
Size: 319 2615 879 492 298 1921
Fragments arranged by size:
2615 1921 879 492 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 1453 6228 1453
Size: 4775 1749
BsaAI yAC'GTr
Cuts at: 2406 3084 3458 3516 3877 2406
Size: 678 374 58 361 5053
Fragments arranged by size:
5053 678 374 361 58
BsaBI GATnn'nnATC
Cuts at: 6 2530 4038 6075 6
Size: 2524 1508 2037 455
Fragments arranged by size:
2524 2037 1508 455
BsaHI Gr'CG_yC
Cuts at: 200 262 1019 1660 2015 2510 2639 2730 2765
Size: 62 757 641 355 495 129 91 35
Cuts at: 2765 3216 3292 3314 3955 4203 4905 6401 200
Size: 451 76 22 641 248 702 1496 323
Fragments arranged by size:
1496 757 702 641 641 495 451 355
323 248 129 91 76 62 35 22
BsaWI w'CCGG_w
Cuts at: 2264 3713 4234 4912 5186 5333 5744 2264
Size: 1449 521 678 274 147 411 3044
Fragments arranged by size:
3044 1449 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 1906 1936 2299 2329 913
Size: 30 963 30 363 30 5108
Fragments arranged by size:
5108 963 363 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 694 937 1000 2437 196
Size: 498 243 63 1437 4283
Fragments arranged by size:
4283 1437 498 243 63
BseYI C'CCAG_C
Cuts at: 148 2154 2229 3247 3377 3949 4707 5284 5780
Size: 2006 75 1018 130 572 758 577 496
Cuts at: 5780 148
Size: 892
Fragments arranged by size:
2006 1018 892 758 577 572 496 130
75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 771 1825 1986 2261 2581 2590 619
Size: 152 1054 161 275 320 9 4553
Fragments arranged by size:
4553 1054 320 275 161 152 9
BsiEI CG_ry'CG
Cuts at: 104 4066 4112 5320 104
Size: 3962 46 1208 1308
Fragments arranged by size:
3962 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1362 1722 2699 3538 4316 4506 5298 1362
Size: 360 977 839 778 190 792 2588
Fragments arranged by size:
2588 977 839 792 778 360 190
BsiWI C'GTAC_G
Cuts at: 140 140
Size: 6524
Bsp1286I G_dGCh'C
Cuts at: 149 217 331 365 650 888 1049 1362 1636
Size: 68 114 34 285 238 161 313 274
Cuts at: 1636 1722 2161 2317 2443 2699 2940 3538 4149
Size: 86 439 156 126 256 241 598 611
Cuts at: 4149 4242 4316 4506 4568 5298 149
Size: 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 611 598 439 313 285 274
256 241 238 190 161 156 126 114
93 86 74 68 62 34
BspEI T'CCGG_A
Cuts at: 5744 5744
Size: 6524
BspHI T'CATG_A
Cuts at: 2945 5700 2945
Size: 2755 3769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 2244 2828 2882 3574 3788 4090 4471 345
Size: 1899 584 54 692 214 302 381 2398
Fragments arranged by size:
2398 1899 692 584 381 302 214 54
BsrBI CCG'CTC
Cuts at: 50 2051 2808 2865 3471 4816 4870 6354 6519
Size: 2001 757 57 606 1345 54 1484 165
Cuts at: 6519 50
Size: 55
Fragments arranged by size:
2001 1484 1345 757 606 165 57 55
54
BsrDI GCAATG_nn'
Cuts at: 4436 4436
Size: 6524
BsrFI r'CCGG_y
Cuts at: 1704 2017 3017 3070 4522 4703 4912 1704
Size: 313 1000 53 1452 181 209 3316
Fragments arranged by size:
3316 1452 1000 313 209 181 53
BssSI C'ACGA_G
Cuts at: 1501 4795 5153 1501
Size: 3294 358 2872
Fragments arranged by size:
3294 2872 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1887 2577 2847 3901 1887
Size: 690 270 1054 4510
Fragments arranged by size:
4510 1054 690 270
BstBI TT'CG_AA
Cuts at: 110 4885 110
Size: 4775 1749
BstEII G'GTnAC_C
Cuts at: 627 2020 627
Size: 1393 5131
BstXI CCAn_nnnn'nTGG
Cuts at: 132 2707 132
Size: 2575 3949
BstYI r'GATC_y
Cuts at: 1 857 1757 3549 4016 4039 4374 4620 5632
Size: 856 900 1792 467 23 335 246 1012
Cuts at: 5632 5718 5730 6076 1
Size: 86 12 346 449
Fragments arranged by size:
1792 1012 900 856 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 1451 1736 2105 2905 1451
Size: 285 369 800 5070
Fragments arranged by size:
5070 800 369 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1337 1716 4474 4655 4818 1337
Size: 379 2758 181 163 3043
Fragments arranged by size:
3043 2758 379 181 163
BtsI GCAGTG_nn'
Cuts at: 814 2145 2333 3767 814
Size: 1331 188 1434 3571
Fragments arranged by size:
3571 1434 1331 188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 549 584 549
Size: 35 6489
DraI TTT'AAA
Cuts at: 2717 3420 3523 2717
Size: 703 103 5718
Fragments arranged by size:
5718 703 103
DraIII CAC_nnn'GTG
Cuts at: 606 1506 606
Size: 900 5624
DrdI GACnn_nn'nnGTC
Cuts at: 1467 1659 3501 4230 5088 1467
Size: 192 1842 729 858 2903
Fragments arranged by size:
2903 1842 858 729 192
EaeI y'GGCC_r
Cuts at: 719 1290 1664 2033 2183 4109 4283 4674 4701
Size: 571 374 369 150 1926 174 391 27
Cuts at: 4701 6046 719
Size: 1345 1197
Fragments arranged by size:
1926 1345 1197 571 391 374 369 174
150 27
EagI C'GGCC_G
Cuts at: 4109 4109
Size: 6524
EarI CTCTTCn'nnn_
Cuts at: 248 1138 2215 4547 4757 248
Size: 890 1077 2332 210 2015
Fragments arranged by size:
2332 2015 1077 890 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 4732 5042 5188 6334 2236
Size: 2496 310 146 1146 2426
Fragments arranged by size:
2496 2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3536 3536
Size: 6524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 940 976 985 1081 1105 2305 2521 4349
Size: 252 36 9 96 24 1200 216 1828
Cuts at: 4349 4781 5528 5796 688
Size: 432 747 268 1416
Fragments arranged by size:
1828 1416 1200 747 432 268 252 216
96 36 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 637 1419 1627 3173 3322 637
Size: 782 208 1546 149 3839
Fragments arranged by size:
3839 1546 782 208 149
EcoO109I rG'GnC_Cy
Cuts at: 474 647 960 1046 1125 1263 1305 1557 1632
Size: 173 313 86 79 138 42 252 75
Cuts at: 1632 3663 474
Size: 2031 3335
Fragments arranged by size:
3335 2031 313 252 173 138 86 79
75 42
EcoRI G'AATT_C
Cuts at: 3528 3528
Size: 6524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1282 3246 4543 1282
Size: 1964 1297 3263
Fragments arranged by size:
3263 1964 1297
FseI GG_CCGG'CC
Cuts at: 3021 3021
Size: 6524
FspI TGC'GCA
Cuts at: 4305 4305
Size: 6524
HaeII r_GCGC'y
Cuts at: 203 1545 2018 2220 2288 2513 2559 3317 3442
Size: 1342 473 202 68 225 46 758 125
Cuts at: 3442 3958 4206 5228 6207 203
Size: 516 248 1022 979 520
Fragments arranged by size:
1342 1022 979 758 520 516 473 248
225 202 125 68 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1447 1479 1906 1938 3277 3309 3406 3438 4366
Size: 32 427 32 1339 32 97 32 928
Cuts at: 4366 4396 4398 4428 1447
Size: 30 2 30 3543
Fragments arranged by size:
3543 1339 928 427 97 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1409 2919 3563 1409
Size: 1510 644 4370
Fragments arranged by size:
4370 1510 644
KasI G'GCGC_C
Cuts at: 199 2014 2509 3313 3954 4202 199
Size: 1815 495 804 641 248 2521
Fragments arranged by size:
2521 1815 804 641 495 248
KpnI G_GTAC'C
Cuts at: 1944 3544 1944
Size: 1600 4924
MluI A'CGCG_T
Cuts at: 5957 5957
Size: 6524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 5195 5379 2321
Size: 2874 184 3466
Fragments arranged by size:
3466 2874 184
MscI TGG'CCA
Cuts at: 2035 4285 2035
Size: 2250 4274
MslI CAynn'nnrTG
Cuts at: 130 1735 2008 2773 2956 3337 4513 4640 130
Size: 1605 273 765 183 381 1176 127 2014
Fragments arranged by size:
2014 1605 1176 765 381 273 183 127
MspA1I CmG'CkG
Cuts at: 819 833 879 1533 1896 2127 2154 2341 2588
Size: 14 46 654 363 231 27 187 247
Cuts at: 2588 3635 3949 4309 5322 5567 819
Size: 1047 314 360 1013 245 1776
Fragments arranged by size:
1776 1047 1013 654 363 360 314 247
245 231 187 46 27 14
NaeI GCC'GGC
Cuts at: 3019 3072 4705 3019
Size: 53 1633 4838
Fragments arranged by size:
4838 1633 53
NarI GG'CG_CC
Cuts at: 200 2015 2510 3314 3955 4203 200
Size: 1815 495 804 641 248 2521
Fragments arranged by size:
2521 1815 804 641 495 248
NgoMIV G'CCGG_C
Cuts at: 3017 3070 4703 3017
Size: 53 1633 4838
Fragments arranged by size:
4838 1633 53
NspI r_CATG'y
Cuts at: 1000 1221 1872 2009 2580 3033 3577 4077 4984
Size: 221 651 137 571 453 544 500 907
Cuts at: 4984 1000
Size: 2540
Fragments arranged by size:
2540 907 651 571 544 500 453 221
137
PasI CC'CwG_GG
Cuts at: 651 2412 651
Size: 1761 4763
PciI A'CATG_T
Cuts at: 2005 4980 2005
Size: 2975 3549
PflMI CCAn_nnn'nTGG
Cuts at: 423 1506 2619 3142 3288 423
Size: 1083 1113 523 146 3659
Fragments arranged by size:
3659 1113 1083 523 146
PfoI T'CCnGG_A
Cuts at: 170 665 824 3240 170
Size: 495 159 2416 3454
Fragments arranged by size:
3454 2416 495 159
PmeI GTTT'AAAC
Cuts at: 3523 3523
Size: 6524
PmlI CAC'GTG
Cuts at: 3084 3084
Size: 6524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 243 5663 5695 211
Size: 32 5420 32 1040
Fragments arranged by size:
5420 1040 32 32
PpuMI rG'GwC_Cy
Cuts at: 474 960 1125 1263 1305 474
Size: 486 165 138 42 5693
Fragments arranged by size:
5693 486 165 138 42
PspOMI G'GGCC_C
Cuts at: 145 361 646 1045 1632 2157 145
Size: 216 285 399 587 525 4512
Fragments arranged by size:
4512 587 525 399 285 216
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2566 2598 2566
Size: 32 6492
PstI C_TGCA'G
Cuts at: 380 496 1883 1892 2255 2340 3177 3571 4256
Size: 116 1387 9 363 85 837 394 685
Cuts at: 4256 380
Size: 2648
Fragments arranged by size:
2648 1387 837 685 394 363 116 85
9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6524
PvuII CAG'CTG
Cuts at: 879 1533 1896 2127 2154 3949 4309 879
Size: 654 363 231 27 1795 360 3094
Fragments arranged by size:
3094 1795 654 363 360 231 27
RsrII CG'GwC_CG
Cuts at: 4719 4719
Size: 6524
SacI G_AGCT'C
Cuts at: 3538 3538
Size: 6524
SalI G'TCGA_C
Cuts at: 1407 3561 1407
Size: 2154 4370
SapI GCTCTTCn'nnn_
Cuts at: 2215 4547 4757 2215
Size: 2332 210 3982
Fragments arranged by size:
3982 2332 210
SbfI CC_TGCA'GG
Cuts at: 2255 3571 2255
Size: 1316 5208
ScaI AGT'ACT
Cuts at: 6041 6041
Size: 6524
SexAI A'CCwGG_T
Cuts at: 2564 3116 2564
Size: 552 5972
SfcI C'TryA_G
Cuts at: 33 376 492 1879 1888 2251 2336 3173 3567
Size: 343 116 1387 9 363 85 837 394
Cuts at: 3567 4252 5245 5436 33
Size: 685 993 191 1121
Fragments arranged by size:
1387 1121 993 837 685 394 363 343
191 116 85 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 281 281
Size: 6524
SfoI GGC'GCC
Cuts at: 201 2016 2511 3315 3956 4204 201
Size: 1815 495 804 641 248 2521
Fragments arranged by size:
2521 1815 804 641 495 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
SgrAI Cr'CCGG_yG
Cuts at: 2017 2017
Size: 6524
SmaI CCC'GGG
Cuts at: 287 311 2933 2971 3546 287
Size: 24 2622 38 575 3265
Fragments arranged by size:
3265 2622 575 38 24
SmlI C'TyrA_G
Cuts at: 781 1100 3679 4594 5086 5348 781
Size: 319 2579 915 492 262 1957
Fragments arranged by size:
2579 1957 915 492 319 262
SnaBI TAC'GTA
Cuts at: 3516 3516
Size: 6524
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6524
SphI G_CATG'C
Cuts at: 1872 3577 4077 1872
Size: 1705 500 4319
Fragments arranged by size:
4319 1705 500
SrfI GCCC'GGGC
Cuts at: 287 311 287
Size: 24 6500
StyI C'CwwG_G
Cuts at: 2186 2700 3405 3658 4012 2186
Size: 514 705 253 354 4698
Fragments arranged by size:
4698 705 514 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1345 2089 2476 3144 4107 4240 4699 4908 1345
Size: 744 387 668 963 133 459 209 2961
Fragments arranged by size:
2961 963 744 668 459 387 209 133
TatI w'GTAC_w
Cuts at: 2573 3032 6039 2573
Size: 459 3007 3058
Fragments arranged by size:
3058 3007 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 913 945 1384 1416 6041 6073 913
Size: 32 439 32 4625 32 1364
Fragments arranged by size:
4625 1364 439 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4321 4321
Size: 6524
XbaI T'CTAG_A
Cuts at: 3555 3555
Size: 6524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 420 3052 3285 4019 420
Size: 2632 233 734 2925
Fragments arranged by size:
2925 2632 734 233
XmaI C'CCGG_G
Cuts at: 285 309 2931 2969 3544 285
Size: 24 2622 38 575 3265
Fragments arranged by size:
3265 2622 575 38 24
XmnI GAAnn'nnTTC
Cuts at: 553 576 1039 2662 553
Size: 23 463 1623 4415
Fragments arranged by size:
4415 1623 463 23
ZraI GAC'GTC
Cuts at: 1661 1661
Size: 6524
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI EcoRV FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
SacII SanDI SspI StuI SwaI XhoI