Restriction map for
pF1KSDA0223
(Circular) (Six-base) MAPSORT of: pF1KSDA0223.seq Check: 9166 from: 1 to: 6545
pF1KSDA0223 6545 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1537 1944 1537
Size: 407 6138
AatII G_ACGT'C
Cuts at: 461 2065 461
Size: 1604 4941
AccI GT'mk_AC
Cuts at: 2288 3152 3583 2288
Size: 864 431 5250
Fragments arranged by size:
5250 864 431
Acc65I G'GTAC_C
Cuts at: 2074 3561 2074
Size: 1487 5058
AclI AA'CG_TT
Cuts at: 6385 6385
Size: 6545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 750 2111 2397 2728 4370 4802 5549 5817 750
Size: 1361 286 331 1642 432 747 268 1478
Fragments arranged by size:
1642 1478 1361 747 432 331 286 268
AfeI AGC'GCT
Cuts at: 955 1360 955
Size: 405 6140
AflII C'TTAA_G
Cuts at: 523 523
Size: 6545
AflIII A'CryG_T
Cuts at: 2535 3897 5001 5978 2535
Size: 1362 1104 977 3102
Fragments arranged by size:
3102 1362 1104 977
AgeI A'CCGG_T
Cuts at: 4933 4933
Size: 6545
AhdI GACnn_n'nnGTC
Cuts at: 1105 1105
Size: 6545
AleI CACnn'nnGTG
Cuts at: 1531 1531
Size: 6545
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1156 1188 1955 1987 1156
Size: 32 767 32 5714
Fragments arranged by size:
5714 767 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1167 1233 1563 1704 2850 3850 5417 1167
Size: 66 330 141 1146 1000 1567 2295
Fragments arranged by size:
2295 1567 1146 1000 330 141 66
ApaI G_GGCC'C
Cuts at: 576 2933 576
Size: 2357 4188
ApaLI G'TGCA_C
Cuts at: 1690 1789 1920 5315 1690
Size: 99 131 3395 2920
Fragments arranged by size:
3395 2920 131 99
ApoI r'AATT_y
Cuts at: 3526 3549 5898 6154 3526
Size: 23 2349 256 3917
Fragments arranged by size:
3917 2349 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 6545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6545
AvaI C'yCGr_G
Cuts at: 210 423 840 2164 2647 2738 2905 3070 3194
Size: 213 417 1324 483 91 167 165 124
Cuts at: 3194 3371 3565 210
Size: 177 194 3190
Fragments arranged by size:
3190 1324 483 417 213 194 177 167
165 124 91
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1562 1595 1562
Size: 33 6512
BamHI G'GATC_C
Cuts at: 3570 3570
Size: 6545
BanI G'GyrC_C
Cuts at: 445 667 1201 1289 1677 2074 3120 3561 3975
Size: 222 534 88 388 397 1046 441 414
Cuts at: 3975 4223 4258 445
Size: 248 35 2732
Fragments arranged by size:
2732 1046 534 441 414 397 388 248
222 88 35
BanII G_rGCy'C
Cuts at: 152 406 576 1121 1784 1808 1903 2684 2807
Size: 254 170 545 663 24 95 781 123
Cuts at: 2807 2933 3505 3559 4589 152
Size: 126 572 54 1030 2108
Fragments arranged by size:
2108 1030 781 663 572 545 254 170
126 123 95 54 24
BbeI G_GCGC'C
Cuts at: 1681 3979 4227 1681
Size: 2298 248 3999
Fragments arranged by size:
3999 2298 248
BbsI GAAGACnn'nnnn_
Cuts at: 908 1552 3036 908
Size: 644 1484 4417
Fragments arranged by size:
4417 1484 644
BbvCI CC'TCA_GC
Cuts at: 506 3104 3280 506
Size: 2598 176 3771
Fragments arranged by size:
3771 2598 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2907 2941 2907
Size: 34 6511
BciVI GTATCCnnnnn_n'
Cuts at: 1563 4443 5204 6545 1563
Size: 2880 761 1341 1563
Fragments arranged by size:
2880 1563 1341 761
BclI T'GATC_A
Cuts at: 4065 4065
Size: 6545
BfrBI ATG'CAT
Cuts at: 609 1665 609
Size: 1056 5489
BglI GCCn_nnn'nGGC
Cuts at: 346 2731 3341 346
Size: 2385 610 3550
Fragments arranged by size:
3550 2385 610
BglII A'GATC_T
Cuts at: 4060 6097 4060
Size: 2037 4508
BlpI GC'TnA_GC
Cuts at: 3657 3657
Size: 6545
Bme1580I G_kGCm'C
Cuts at: 576 886 1204 1299 1694 1793 1848 1924 1933
Size: 310 318 95 395 99 55 76 9
Cuts at: 1933 2186 2372 2450 2933 3270 4170 4263 5319
Size: 253 186 78 483 337 900 93 1056
Cuts at: 5319 576
Size: 1802
Fragments arranged by size:
1802 1056 900 483 395 337 318 310
253 186 99 95 93 78 76 55
9
BmgBI CAC'GTC
Cuts at: 492 942 1812 492
Size: 450 870 5225
Fragments arranged by size:
5225 870 450
BmrI ACTGGGnnnn_n'
Cuts at: 394 2180 3508 3757 3838 4173 6317 394
Size: 1786 1328 249 81 335 2144 622
Fragments arranged by size:
2144 1786 1328 622 335 249 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 489 3252 4912 489
Size: 2763 1660 2122
Fragments arranged by size:
2763 2122 1660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 286 739 766 1054 1072 1177 1189 1498
Size: 169 453 27 288 18 105 12 309
Cuts at: 1498 1585 2365 3151 3310 3379 117
Size: 87 780 786 159 69 3283
Fragments arranged by size:
3283 786 780 453 309 288 169 159
105 87 69 27 18 12
Bpu10I CC'TnA_GC
Cuts at: 328 506 3104 3280 328
Size: 178 2598 176 3593
Fragments arranged by size:
3593 2598 178 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 535 574 2608 3721 4600 5092 5390 295
Size: 240 39 2034 1113 879 492 298 1450
Fragments arranged by size:
2034 1450 1113 879 492 298 240 39
BsaI GGTCTCn'nnnn_
Cuts at: 3058 6249 3058
Size: 3191 3354
BsaAI yAC'GTr
Cuts at: 1353 1755 3537 3898 1353
Size: 402 1782 361 4000
Fragments arranged by size:
4000 1782 402 361
BsaBI GATnn'nnATC
Cuts at: 6 4059 6096 6
Size: 4053 2037 455
Fragments arranged by size:
4053 2037 455
BsaHI Gr'CG_yC
Cuts at: 290 458 911 1678 2062 2734 3976 4224 4926
Size: 168 453 767 384 672 1242 248 702
Cuts at: 4926 6422 290
Size: 1496 413
Fragments arranged by size:
1496 1242 767 702 672 453 413 384
248 168
BsaWI w'CCGG_w
Cuts at: 398 2240 3734 4255 4933 5207 5354 5765 398
Size: 1842 1494 521 678 274 147 411 1178
Fragments arranged by size:
1842 1494 1178 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 520 550 1045 1075 2533 2563 520
Size: 30 495 30 1458 30 4502
Fragments arranged by size:
4502 1458 495 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 946 1420 1423 1495 1498 2107 2967 3064 3067
Size: 474 3 72 3 609 860 97 3
Cuts at: 3067 3076 3244 3364 946
Size: 9 168 120 4127
Fragments arranged by size:
4127 860 609 474 168 120 97 72
9 3 3 3
BseYI C'CCAG_C
Cuts at: 196 277 892 3407 3970 4728 5305 5801 196
Size: 81 615 2515 563 758 577 496 940
Fragments arranged by size:
2515 940 758 615 577 563 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 1375 3204 1210
Size: 165 1829 4551
Fragments arranged by size:
4551 1829 165
BsiEI CG_ry'CG
Cuts at: 104 1885 4087 4133 5341 104
Size: 1781 2202 46 1208 1308
Fragments arranged by size:
2202 1781 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 152 1694 1793 1924 2684 2807 3559 4337 4527
Size: 1542 99 131 760 123 752 778 190
Cuts at: 4527 5319 152
Size: 792 1378
Fragments arranged by size:
1542 1378 792 778 760 752 190 131
123 99
BsmBI CGTCTCn'nnnn_
Cuts at: 643 742 1818 2341 643
Size: 99 1076 523 4847
Fragments arranged by size:
4847 1076 523 99
Bsp1286I G_dGCh'C
Cuts at: 152 406 576 886 1121 1204 1299 1694 1784
Size: 254 170 310 235 83 95 395 90
Cuts at: 1784 1793 1808 1848 1903 1924 1933 2186 2372
Size: 9 15 40 55 21 9 253 186
Cuts at: 2372 2450 2684 2807 2933 3270 3505 3559 4170
Size: 78 234 123 126 337 235 54 611
Cuts at: 4170 4263 4337 4527 4589 5319 152
Size: 93 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 395 337 310 254 253
235 235 234 190 186 170 126 123
95 93 90 83 78 74 62 55
54 40 21 15 9 9
BspEI T'CCGG_A
Cuts at: 2240 5765 2240
Size: 3525 3020
BspHI T'CATG_A
Cuts at: 152 5721 152
Size: 5569 976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1337 1537 1944 2642 2779 2825 2858 3595 3809
Size: 200 407 698 137 46 33 737 214
Cuts at: 3809 4111 4492 1337
Size: 302 381 3390
Fragments arranged by size:
3390 737 698 407 381 302 214 200
137 46 33
BsrBI CCG'CTC
Cuts at: 50 1131 1989 2058 2484 2655 4837 4891 6375
Size: 1081 858 69 426 171 2182 54 1484
Cuts at: 6375 6540 50
Size: 165 55
Fragments arranged by size:
2182 1484 1081 858 426 171 165 69
55 54
BsrDI GCAATG_nn'
Cuts at: 674 4457 674
Size: 3783 2762
BsrFI r'CCGG_y
Cuts at: 448 664 913 2172 2234 2744 4543 4724 4933
Size: 216 249 1259 62 510 1799 181 209
Cuts at: 4933 448
Size: 2060
Fragments arranged by size:
2060 1799 1259 510 249 216 209 181
62
BssHII G'CGCG_C
Cuts at: 1424 1424
Size: 6545
BssSI C'ACGA_G
Cuts at: 577 1255 1390 2203 2677 4816 5174 577
Size: 678 135 813 474 2139 358 1948
Fragments arranged by size:
2139 1948 813 678 474 358 135
BstAPI GCAn_nnn'nTGC
Cuts at: 2850 3922 2850
Size: 1072 5473
BstBI TT'CG_AA
Cuts at: 110 4906 110
Size: 4796 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 999 1458 3270 999
Size: 459 1812 4274
Fragments arranged by size:
4274 1812 459
BstYI r'GATC_y
Cuts at: 1 1595 3570 4037 4060 4395 4641 5653 5739
Size: 1594 1975 467 23 335 246 1012 86
Cuts at: 5739 5751 6097 1
Size: 12 346 449
Fragments arranged by size:
1975 1594 1012 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 568 644 851 2087 2947 3480 568
Size: 76 207 1236 860 533 3633
Fragments arranged by size:
3633 1236 860 533 207 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4495 4676 4839 4495
Size: 181 163 6201
Fragments arranged by size:
6201 181 163
BtsI GCAGTG_nn'
Cuts at: 830 882 979 2777 3351 3460 3788 830
Size: 52 97 1798 574 109 328 3587
Fragments arranged by size:
3587 1798 574 328 109 97 52
DraI TTT'AAA
Cuts at: 3544 3544
Size: 6545
DrdI GACnn_nn'nnGTC
Cuts at: 4251 5109 4251
Size: 858 5687
EaeI y'GGCC_r
Cuts at: 1001 1176 1394 1445 1514 1535 1637 1882 1984
Size: 175 218 51 69 21 102 245 102
Cuts at: 1984 2177 2354 2403 2693 2944 4130 4304 4695
Size: 193 177 49 290 251 1186 174 391
Cuts at: 4695 4722 6067 1001
Size: 27 1345 1479
Fragments arranged by size:
1479 1345 1186 391 290 251 245 218
193 177 175 174 102 102 69 51
49 27 21
EagI C'GGCC_G
Cuts at: 1882 4130 1882
Size: 2248 4297
EarI CTCTTCn'nnn_
Cuts at: 1846 2306 4568 4778 1846
Size: 460 2262 210 3613
Fragments arranged by size:
3613 2262 460 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 502 1333 1417 1516 1806 2188 2656 3190 3504
Size: 831 84 99 290 382 468 534 314
Cuts at: 3504 4753 5063 5209 6355 502
Size: 1249 310 146 1146 692
Fragments arranged by size:
1249 1146 831 692 534 468 382 314
310 290 146 99 84
EcoICRI GAG'CTC
Cuts at: 150 2682 2805 3557 150
Size: 2532 123 752 3138
Fragments arranged by size:
3138 2532 752 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 286 739 750 766 1054 1072 1177 1189
Size: 169 453 11 16 288 18 105 12
Cuts at: 1189 1498 1585 2111 2365 2397 2728 3151 3310
Size: 309 87 526 254 32 331 423 159
Cuts at: 3310 3379 4370 4802 5549 5817 117
Size: 69 991 432 747 268 845
Fragments arranged by size:
991 845 747 526 453 432 423 331
309 288 268 254 169 159 105 87
69 32 18 16 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 553 589 1159 1333 2033 3229 553
Size: 36 570 174 700 1196 3869
Fragments arranged by size:
3869 1196 700 570 174 36
EcoO109I rG'GnC_Cy
Cuts at: 281 305 510 741 1017 1071 1164 2814 3456
Size: 24 205 231 276 54 93 1650 642
Cuts at: 3456 3684 281
Size: 228 3142
Fragments arranged by size:
3142 1650 642 276 231 228 205 93
54 24
EcoRI G'AATT_C
Cuts at: 3526 3549 3526
Size: 23 6522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1401 1875 4564 1401
Size: 474 2689 3382
Fragments arranged by size:
3382 2689 474
FspI TGC'GCA
Cuts at: 1333 2497 4326 1333
Size: 1164 1829 3552
Fragments arranged by size:
3552 1829 1164
HaeII r_GCGC'y
Cuts at: 957 1362 1588 1681 2435 2494 3979 4227 5249
Size: 405 226 93 754 59 1485 248 1022
Cuts at: 5249 6228 957
Size: 979 1274
Fragments arranged by size:
1485 1274 1022 979 754 405 248 226
93 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1091 1123 1306 1338 2047 2079 2197 2229 3073
Size: 32 183 32 709 32 118 32 844
Cuts at: 3073 3105 3202 3232 3234 3264 4387 4417 4419
Size: 32 97 30 2 30 1123 30 2
Cuts at: 4419 4449 1091
Size: 30 3187
Fragments arranged by size:
3187 1123 844 709 183 118 97 32
32 32 32 32 30 30 30 30
2 2
HincII GTy'rAC
Cuts at: 3584 3584
Size: 6545
KasI G'GCGC_C
Cuts at: 1677 3975 4223 1677
Size: 2298 248 3999
Fragments arranged by size:
3999 2298 248
KpnI G_GTAC'C
Cuts at: 2078 3565 2078
Size: 1487 5058
MluI A'CGCG_T
Cuts at: 2535 5978 2535
Size: 3443 3102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1349 3116 3230 3464 5216 5400 1349
Size: 1767 114 234 1752 184 2494
Fragments arranged by size:
2494 1767 1752 234 184 114
MscI TGG'CCA
Cuts at: 1003 1396 1516 2356 2695 4306 1003
Size: 393 120 840 339 1611 3242
Fragments arranged by size:
3242 1611 840 393 339 120
MslI CAynn'nnrTG
Cuts at: 1531 4534 4661 1531
Size: 3003 127 3415
Fragments arranged by size:
3415 3003 127
MspA1I CmG'CkG
Cuts at: 277 570 633 1683 1764 1770 2397 3159 3341
Size: 293 63 1050 81 6 627 762 182
Cuts at: 3341 3357 3656 3970 4330 5343 5588 277
Size: 16 299 314 360 1013 245 1234
Fragments arranged by size:
1234 1050 1013 762 627 360 314 299
293 245 182 81 63 16 6
NaeI GCC'GGC
Cuts at: 450 666 915 2746 4726 450
Size: 216 249 1831 1980 2269
Fragments arranged by size:
2269 1980 1831 249 216
NarI GG'CG_CC
Cuts at: 1678 3976 4224 1678
Size: 2298 248 3999
Fragments arranged by size:
3999 2298 248
NcoI C'CATG_G
Cuts at: 851 851
Size: 6545
NdeI CA'TA_TG
Cuts at: 1667 1667
Size: 6545
NgoMIV G'CCGG_C
Cuts at: 448 664 913 2744 4724 448
Size: 216 249 1831 1980 2269
Fragments arranged by size:
2269 1980 1831 249 216
NsiI A_TGCA'T
Cuts at: 611 1667 611
Size: 1056 5489
NspI r_CATG'y
Cuts at: 1128 3598 4098 5005 1128
Size: 2470 500 907 2668
Fragments arranged by size:
2668 2470 907 500
PasI CC'CwG_GG
Cuts at: 3270 3270
Size: 6545
PciI A'CATG_T
Cuts at: 5001 5001
Size: 6545
PflMI CCAn_nnn'nTGG
Cuts at: 483 852 1185 1826 2113 483
Size: 369 333 641 287 4915
Fragments arranged by size:
4915 641 369 333 287
PfoI T'CCnGG_A
Cuts at: 133 376 1598 3287 133
Size: 243 1222 1689 3391
Fragments arranged by size:
3391 1689 1222 243
PmeI GTTT'AAAC
Cuts at: 3544 3544
Size: 6545
PmlI CAC'GTG
Cuts at: 1755 1755
Size: 6545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2853 5684 5716 2821
Size: 32 2831 32 3650
Fragments arranged by size:
3650 2831 32 32
PpuMI rG'GwC_Cy
Cuts at: 281 305 1017 1164 2814 281
Size: 24 712 147 1650 4012
Fragments arranged by size:
4012 1650 712 147 24
PspOMI G'GGCC_C
Cuts at: 572 2929 572
Size: 2357 4188
PspXI vC'TCGA_Gb
Cuts at: 840 840
Size: 6545
PstI C_TGCA'G
Cuts at: 1079 1099 1769 2396 2846 3164 3455 3511 3592
Size: 20 670 627 450 318 291 56 81
Cuts at: 3592 4277 1079
Size: 685 3347
Fragments arranged by size:
3347 685 670 627 450 318 291 81
56 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6545
PvuII CAG'CTG
Cuts at: 1764 2397 3357 3970 4330 1764
Size: 633 960 613 360 3979
Fragments arranged by size:
3979 960 633 613 360
RsrII CG'GwC_CG
Cuts at: 4740 4740
Size: 6545
SacI G_AGCT'C
Cuts at: 152 2684 2807 3559 152
Size: 2532 123 752 3138
Fragments arranged by size:
3138 2532 752 123
SacII CC_GC'GG
Cuts at: 571 571
Size: 6545
SalI G'TCGA_C
Cuts at: 3582 3582
Size: 6545
SanDI GG'GwC_CC
Cuts at: 305 305
Size: 6545
SapI GCTCTTCn'nnn_
Cuts at: 1846 4568 4778 1846
Size: 2722 210 3613
Fragments arranged by size:
3613 2722 210
SbfI CC_TGCA'GG
Cuts at: 3511 3592 3511
Size: 81 6464
ScaI AGT'ACT
Cuts at: 6062 6062
Size: 6545
SexAI A'CCwGG_T
Cuts at: 1967 1967
Size: 6545
SfcI C'TryA_G
Cuts at: 33 949 1075 1095 1765 2392 2842 3160 3451
Size: 916 126 20 670 627 450 318 291
Cuts at: 3451 3507 3588 4273 5266 5457 33
Size: 56 81 685 993 191 1121
Fragments arranged by size:
1121 993 916 685 670 627 450 318
291 191 126 81 56 20
SfoI GGC'GCC
Cuts at: 1679 3977 4225 1679
Size: 2298 248 3999
Fragments arranged by size:
3999 2298 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6545
SmaI CCC'GGG
Cuts at: 425 2740 2907 3072 3373 3567 425
Size: 2315 167 165 301 194 3403
Fragments arranged by size:
3403 2315 301 194 167 165
SmlI C'TyrA_G
Cuts at: 310 514 523 553 840 2623 3700 4615 5107
Size: 204 9 30 287 1783 1077 915 492
Cuts at: 5107 5369 310
Size: 262 1486
Fragments arranged by size:
1783 1486 1077 915 492 287 262 204
30 9
SnaBI TAC'GTA
Cuts at: 3537 3537
Size: 6545
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6545
SphI G_CATG'C
Cuts at: 3598 4098 3598
Size: 500 6045
SrfI GCCC'GGGC
Cuts at: 425 425
Size: 6545
StuI AGG'CCT
Cuts at: 1285 2557 2596 3235 1285
Size: 1272 39 639 4595
Fragments arranged by size:
4595 1272 639 39
StyI C'CwwG_G
Cuts at: 680 851 1067 1397 1574 1940 2216 2501 2696
Size: 171 216 330 177 366 276 285 195
Cuts at: 2696 2726 3679 4033 680
Size: 30 953 354 3192
Fragments arranged by size:
3192 953 366 354 330 285 276 216
195 177 171 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 577 784 4128 4261 4720 4929 577
Size: 207 3344 133 459 209 2193
Fragments arranged by size:
3344 2193 459 209 207 133
TatI w'GTAC_w
Cuts at: 1007 6060 1007
Size: 5053 1492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2533 2565 6062 6094 2533
Size: 32 3497 32 2984
Fragments arranged by size:
3497 2984 32 32
Tth111I GACn'n_nGTC
Cuts at: 460 4342 460
Size: 3882 2663
XbaI T'CTAG_A
Cuts at: 3576 3576
Size: 6545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1146 1455 2094 3318 4040 1146
Size: 309 639 1224 722 3651
Fragments arranged by size:
3651 1224 722 639 309
XhoI C'TCGA_G
Cuts at: 840 840
Size: 6545
XmaI C'CCGG_G
Cuts at: 423 2738 2905 3070 3371 3565 423
Size: 2315 167 165 301 194 3403
Fragments arranged by size:
3403 2315 301 194 167 165
ZraI GAC'GTC
Cuts at: 459 2063 459
Size: 1604 4941
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AlfI AscI AvrII BmtI BsiWI BsmI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PshAI
PsiI PsrI SfiI SgrAI SspI SwaI XmnI