Restriction map for
pF1KSDA0224
(Circular) (Six-base) MAPSORT of: pF1KSDA0224.seq Check: 9855 from: 1 to: 6820
pF1KSDA0224 6820 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1196 2313 1196
Size: 1117 5703
AatII G_ACGT'C
Cuts at: 2557 2557
Size: 6820
AccI GT'mk_AC
Cuts at: 763 3727 3856 763
Size: 2964 129 3727
Fragments arranged by size:
3727 2964 129
Acc65I G'GTAC_C
Cuts at: 3834 3834
Size: 6820
AclI AA'CG_TT
Cuts at: 6660 6660
Size: 6820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1967 2179 2253 4643 5075 5824 6092 1967
Size: 212 74 2390 432 749 268 2695
Fragments arranged by size:
2695 2390 749 432 268 212 74
AfeI AGC'GCT
Cuts at: 1252 2095 1252
Size: 843 5977
AflIII A'CryG_T
Cuts at: 4170 5276 6253 4170
Size: 1106 977 4737
Fragments arranged by size:
4737 1106 977
AgeI A'CCGG_T
Cuts at: 5208 5208
Size: 6820
AleI CACnn'nnGTG
Cuts at: 2063 2110 2770 2063
Size: 47 660 6113
Fragments arranged by size:
6113 660 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1655 3430 1655
Size: 1775 5045
AlwNI CAG_nnn'CTG
Cuts at: 1188 1719 1746 1830 2841 4123 5692 1188
Size: 531 27 84 1011 1282 1569 2316
Fragments arranged by size:
2316 1569 1282 1011 531 84 27
ApaI G_GGCC'C
Cuts at: 2343 2710 2922 3562 2343
Size: 367 212 640 5601
Fragments arranged by size:
5601 640 367 212
ApaLI G'TGCA_C
Cuts at: 1351 2326 5590 1351
Size: 975 3264 2581
Fragments arranged by size:
3264 2581 975
ApoI r'AATT_y
Cuts at: 1034 1678 3822 6173 6429 1034
Size: 644 2144 2351 256 1425
Fragments arranged by size:
2351 2144 1425 644 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6820
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6820
AvaI C'yCGr_G
Cuts at: 464 715 875 2776 2827 2914 3838 464
Size: 251 160 1901 51 87 924 3446
Fragments arranged by size:
3446 1901 924 251 160 87 51
BamHI G'GATC_C
Cuts at: 2018 2909 3843 2018
Size: 891 934 4995
Fragments arranged by size:
4995 934 891
BanI G'GyrC_C
Cuts at: 1362 3834 4248 4496 4531 1362
Size: 2472 414 248 35 3651
Fragments arranged by size:
3651 2472 414 248 35
BanII G_rGCy'C
Cuts at: 724 2343 2710 2759 2922 3264 3562 3681 3770
Size: 1619 367 49 163 342 298 119 89
Cuts at: 3770 3832 4862 724
Size: 62 1030 2682
Fragments arranged by size:
2682 1619 1030 367 342 298 163 119
89 62 49
BbeI G_GCGC'C
Cuts at: 4252 4500 4252
Size: 248 6572
BbsI GAAGACnn'nnnn_
Cuts at: 231 975 1017 1383 1979 2607 231
Size: 744 42 366 596 628 4444
Fragments arranged by size:
4444 744 628 596 366 42
BbvCI CC'TCA_GC
Cuts at: 401 401
Size: 6820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1075 1109 1761 1795 1075
Size: 34 652 34 6100
Fragments arranged by size:
6100 652 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4716 5479 6820 4716
Size: 763 1341 4716
Fragments arranged by size:
4716 1341 763
BclI T'GATC_A
Cuts at: 1997 3108 4338 1997
Size: 1111 1230 4479
Fragments arranged by size:
4479 1230 1111
BglI GCCn_nnn'nGGC
Cuts at: 3713 3713
Size: 6820
BglII A'GATC_T
Cuts at: 2648 3734 4333 6372 2648
Size: 1086 599 2039 3096
Fragments arranged by size:
3096 2039 1086 599
BlpI GC'TnA_GC
Cuts at: 1220 1514 3930 1220
Size: 294 2416 4110
Fragments arranged by size:
4110 2416 294
Bme1580I G_kGCm'C
Cuts at: 1355 2330 2343 2710 2777 2922 3562 4443 4536
Size: 975 13 367 67 145 640 881 93
Cuts at: 4536 5594 1355
Size: 1058 2581
Fragments arranged by size:
2581 1058 975 881 640 367 145 93
67 13
BmgBI CAC'GTC
Cuts at: 1015 2112 1015
Size: 1097 5723
BmrI ACTGGGnnnn_n'
Cuts at: 280 378 1536 1558 3330 4030 4111 4446 6592
Size: 98 1158 22 1772 700 81 335 2146
Cuts at: 6592 280
Size: 508
Fragments arranged by size:
2146 1772 1158 700 508 335 98 81
22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 165 2053 2409 3283 3648 5185 165
Size: 1888 356 874 365 1537 1800
Fragments arranged by size:
1888 1800 1537 874 365 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1723 2366 2437 2446 2467 2492 3728 1318
Size: 405 643 71 9 21 25 1236 4410
Fragments arranged by size:
4410 1236 643 405 71 25 21 9
Bpu10I CC'TnA_GC
Cuts at: 401 3230 401
Size: 2829 3991
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 783 2803 3253 3361 3994 4873 5367 5665 783
Size: 2020 450 108 633 879 494 298 1938
Fragments arranged by size:
2020 1938 879 633 494 450 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 6524 6524
Size: 6820
BsaAI yAC'GTr
Cuts at: 1167 2295 3396 3810 4171 1167
Size: 1128 1101 414 361 3816
Fragments arranged by size:
3816 1128 1101 414 361
BsaBI GATnn'nnATC
Cuts at: 6 3001 4332 6371 6
Size: 2995 1331 2039 455
Fragments arranged by size:
2995 2039 1331 455
BsaHI Gr'CG_yC
Cuts at: 832 1565 2554 4249 4497 5201 6697 832
Size: 733 989 1695 248 704 1496 955
Fragments arranged by size:
1695 1496 989 955 733 704 248
BsaWI w'CCGG_w
Cuts at: 437 4007 4528 5208 5482 5629 6040 437
Size: 3570 521 680 274 147 411 1217
Fragments arranged by size:
3570 1217 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2080 2110 2080
Size: 30 6790
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 345 786 1033 1066 1069 1143 1185 1960
Size: 17 441 247 33 3 74 42 775
Cuts at: 1960 3112 3115 3658 3661 3679 328
Size: 1152 3 543 3 18 3469
Fragments arranged by size:
3469 1152 775 543 441 247 74 42
33 18 17 3 3 3
BseYI C'CCAG_C
Cuts at: 1253 1874 3066 3555 4243 5001 5580 6076 1253
Size: 621 1192 489 688 758 579 496 1997
Fragments arranged by size:
1997 1192 758 688 621 579 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 1962 2854 3304 1756
Size: 206 892 450 5272
Fragments arranged by size:
5272 892 450 206
BsiEI CG_ry'CG
Cuts at: 104 1095 4360 4406 5616 104
Size: 991 3265 46 1210 1308
Fragments arranged by size:
3265 1308 1210 991 46
BsiHKAI G_wGCw'C
Cuts at: 1355 2117 2330 2759 3264 3832 4610 4800 5594
Size: 762 213 429 505 568 778 190 794
Cuts at: 5594 1355
Size: 2581
Fragments arranged by size:
2581 794 778 762 568 505 429 213
190
BsmBI CGTCTCn'nnnn_
Cuts at: 1786 2545 2561 1786
Size: 759 16 6045
Fragments arranged by size:
6045 759 16
Bsp1286I G_dGCh'C
Cuts at: 724 1355 2117 2330 2343 2710 2759 2777 2922
Size: 631 762 213 13 367 49 18 145
Cuts at: 2922 3264 3562 3681 3770 3832 4443 4536 4610
Size: 342 298 119 89 62 611 93 74
Cuts at: 4610 4800 4862 5594 724
Size: 190 62 732 1950
Fragments arranged by size:
1950 762 732 631 611 367 342 298
213 190 145 119 93 89 74 62
62 49 18 13
BspEI T'CCGG_A
Cuts at: 437 6040 437
Size: 5603 1217
BspHI T'CATG_A
Cuts at: 3494 5996 3494
Size: 2502 4318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1196 1727 1838 2313 2849 3179 3868 4082 4384
Size: 531 111 475 536 330 689 214 302
Cuts at: 4384 4765 1196
Size: 381 3251
Fragments arranged by size:
3251 689 536 531 475 381 330 302
214 111
BsrBI CCG'CTC
Cuts at: 50 534 540 642 855 1059 5110 5164 6650
Size: 484 6 102 213 204 4051 54 1486
Cuts at: 6650 6815 50
Size: 165 55
Fragments arranged by size:
4051 1486 484 213 204 165 102 55
54 6
BsrDI GCAATG_nn'
Cuts at: 1451 4730 1451
Size: 3279 3541
BsrFI r'CCGG_y
Cuts at: 2695 4816 4997 5208 2695
Size: 2121 181 211 4307
Fragments arranged by size:
4307 2121 211 181
BssSI C'ACGA_G
Cuts at: 2089 3306 3484 5089 5449 2089
Size: 1217 178 1605 360 3460
Fragments arranged by size:
3460 1605 1217 360 178
BstAPI GCAn_nnn'nTGC
Cuts at: 1188 1719 1746 1830 2319 2841 4195 1188
Size: 531 27 84 489 522 1354 3813
Fragments arranged by size:
3813 1354 531 522 489 84 27
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6820
BstEII G'GTnAC_C
Cuts at: 415 2388 415
Size: 1973 4847
BstXI CCAn_nnnn'nTGG
Cuts at: 490 1284 1902 2076 2239 490
Size: 794 618 174 163 5071
Fragments arranged by size:
5071 794 618 174 163
BstYI r'GATC_y
Cuts at: 1 2018 2648 2780 2909 3035 3734 3843 4310
Size: 2017 630 132 129 126 699 109 467
Cuts at: 4310 4333 4668 4914 5928 6014 6026 6372 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
2017 1014 699 630 467 449 346 335
246 132 129 126 109 86 23 12
BtgI C'CryG_G
Cuts at: 125 3638 3770 125
Size: 3513 132 3175
Fragments arranged by size:
3513 3175 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4768 4949 5112 4768
Size: 181 163 6476
Fragments arranged by size:
6476 181 163
BtsI GCAGTG_nn'
Cuts at: 1072 1680 1739 2312 3181 3523 3723 4061 1072
Size: 608 59 573 869 342 200 338 3831
Fragments arranged by size:
3831 869 608 573 342 338 200 59
ClaI AT'CG_AT
Cuts at: 159 2588 159
Size: 2429 4391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2526 2561 2916 2951 2526
Size: 35 355 35 6395
Fragments arranged by size:
6395 355 35 35
DraI TTT'AAA
Cuts at: 3817 3817
Size: 6820
DrdI GACnn_nn'nnGTC
Cuts at: 4524 5384 4524
Size: 860 5960
EaeI y'GGCC_r
Cuts at: 221 426 1331 1445 1904 2241 2370 2798 3659
Size: 205 905 114 459 337 129 428 861
Cuts at: 3659 4403 4577 4968 4995 6342 221
Size: 744 174 391 27 1347 699
Fragments arranged by size:
1347 905 861 744 699 459 428 391
337 205 174 129 114 27
EagI C'GGCC_G
Cuts at: 4403 4403
Size: 6820
EarI CTCTTCn'nnn_
Cuts at: 368 401 497 770 1313 1574 1648 1916 2272
Size: 33 96 273 543 261 74 268 356
Cuts at: 2272 4841 5051 368
Size: 2569 210 2137
Fragments arranged by size:
2569 2137 543 356 273 268 261 210
96 74 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3565 5026 5338 5484 6630 3565
Size: 1461 312 146 1146 3755
Fragments arranged by size:
3755 1461 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2757 3262 3830 2757
Size: 505 568 5747
Fragments arranged by size:
5747 568 505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1723 1967 2179 2253 2366 2437 2446 2467
Size: 405 244 212 74 113 71 9 21
Cuts at: 2467 2492 3728 4643 5075 5824 6092 1318
Size: 25 1236 915 432 749 268 2046
Fragments arranged by size:
2046 1236 915 749 432 405 268 244
212 113 74 71 25 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1942 1942
Size: 6820
EcoO109I rG'GnC_Cy
Cuts at: 2339 2340 2838 2918 2919 3559 3957 2339
Size: 1 498 80 1 640 398 5202
Fragments arranged by size:
5202 640 498 398 80 1 1
EcoRI G'AATT_C
Cuts at: 3822 3822
Size: 6820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2334 4837 2334
Size: 2503 4317
FspI TGC'GCA
Cuts at: 4599 4599
Size: 6820
HaeII r_GCGC'y
Cuts at: 807 1254 2097 2151 2744 3658 4252 4500 5524
Size: 447 843 54 593 914 594 248 1024
Cuts at: 5524 6503 807
Size: 979 1124
Fragments arranged by size:
1124 1024 979 914 843 594 593 447
248 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 463 495 670 702 3726 3758 4660 4690 4692
Size: 32 175 32 3024 32 902 30 2
Cuts at: 4692 4722 463
Size: 30 2561
Fragments arranged by size:
3024 2561 902 175 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2259 2568 3857 2259
Size: 309 1289 5222
Fragments arranged by size:
5222 1289 309
KasI G'GCGC_C
Cuts at: 4248 4496 4248
Size: 248 6572
KpnI G_GTAC'C
Cuts at: 3838 3838
Size: 6820
MluI A'CGCG_T
Cuts at: 6253 6253
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5491 5675 5491
Size: 184 6636
MscI TGG'CCA
Cuts at: 428 1447 1906 2372 2800 4579 428
Size: 1019 459 466 428 1779 2669
Fragments arranged by size:
2669 1779 1019 466 459 428
MslI CAynn'nnrTG
Cuts at: 488 1838 1900 2063 2074 2110 2386 2770 3280
Size: 1350 62 163 11 36 276 384 510
Cuts at: 3280 3412 4807 4934 488
Size: 132 1395 127 2374
Fragments arranged by size:
2374 1395 1350 510 384 276 163 132
127 62 36 11
MspA1I CmG'CkG
Cuts at: 1143 1224 1818 2469 2682 2976 3291 3529 3672
Size: 81 594 651 213 294 315 238 143
Cuts at: 3672 3929 4243 4603 5618 5863 1143
Size: 257 314 360 1015 245 2100
Fragments arranged by size:
2100 1015 651 594 360 315 314 294
257 245 238 213 143 81
NaeI GCC'GGC
Cuts at: 2697 4999 2697
Size: 2302 4518
NarI GG'CG_CC
Cuts at: 4249 4497 4249
Size: 248 6572
NcoI C'CATG_G
Cuts at: 125 3638 3770 125
Size: 3513 132 3175
Fragments arranged by size:
3513 3175 132
NgoMIV G'CCGG_C
Cuts at: 2695 4997 2695
Size: 2302 4518
NruI TCG'CGA
Cuts at: 5181 5181
Size: 6820
NspI r_CATG'y
Cuts at: 238 1272 2886 3871 4371 5280 238
Size: 1034 1614 985 500 909 1778
Fragments arranged by size:
1778 1614 1034 985 909 500
PasI CC'CwG_GG
Cuts at: 3711 3711
Size: 6820
PciI A'CATG_T
Cuts at: 5276 5276
Size: 6820
PflMI CCAn_nnn'nTGG
Cuts at: 162 1296 2406 2859 3203 162
Size: 1134 1110 453 344 3779
Fragments arranged by size:
3779 1134 1110 453 344
PfoI T'CCnGG_A
Cuts at: 1700 2039 2129 3116 3248 1700
Size: 339 90 987 132 5272
Fragments arranged by size:
5272 987 339 132 90
PmeI GTTT'AAAC
Cuts at: 3817 3817
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5959 5991 5959
Size: 32 6788
PpuMI rG'GwC_Cy
Cuts at: 2838 2838
Size: 6820
PspOMI G'GGCC_C
Cuts at: 2339 2706 2918 3558 2339
Size: 367 212 640 5601
Fragments arranged by size:
5601 640 367 212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3175 3207 3175
Size: 32 6788
PstI C_TGCA'G
Cuts at: 2307 2648 2849 3028 3176 3865 4550 2307
Size: 341 201 179 148 689 685 4577
Fragments arranged by size:
4577 689 685 341 201 179 148
PvuI CG_AT'CG
Cuts at: 104 1095 104
Size: 991 5829
PvuII CAG'CTG
Cuts at: 1818 2469 3672 4243 4603 1818
Size: 651 1203 571 360 4035
Fragments arranged by size:
4035 1203 651 571 360
RsrII CG'GwC_CG
Cuts at: 5013 5013
Size: 6820
SacI G_AGCT'C
Cuts at: 2759 3264 3832 2759
Size: 505 568 5747
Fragments arranged by size:
5747 568 505
SalI G'TCGA_C
Cuts at: 3855 3855
Size: 6820
SapI GCTCTTCn'nnn_
Cuts at: 1574 4841 5051 1574
Size: 3267 210 3343
Fragments arranged by size:
3343 3267 210
SbfI CC_TGCA'GG
Cuts at: 3865 3865
Size: 6820
ScaI AGT'ACT
Cuts at: 907 6337 907
Size: 5430 1390
SfcI C'TryA_G
Cuts at: 33 1620 2061 2303 2644 2845 3024 3078 3172
Size: 1587 441 242 341 201 179 54 94
Cuts at: 3172 3570 3861 4546 5541 5732 33
Size: 398 291 685 995 191 1121
Fragments arranged by size:
1587 1121 995 685 441 398 341 291
242 201 191 179 94 54
SfoI GGC'GCC
Cuts at: 4250 4498 4250
Size: 248 6572
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6820
SmaI CCC'GGG
Cuts at: 3840 3840
Size: 6820
SmlI C'TyrA_G
Cuts at: 798 875 2818 3268 3376 3973 4888 5382 5644
Size: 77 1943 450 108 597 915 494 262
Cuts at: 5644 798
Size: 1974
Fragments arranged by size:
1974 1943 915 597 494 450 262 108
77
SnaBI TAC'GTA
Cuts at: 3810 3810
Size: 6820
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6820
SphI G_CATG'C
Cuts at: 1272 3871 4371 1272
Size: 2599 500 3721
Fragments arranged by size:
3721 2599 500
SspI AAT'ATT
Cuts at: 2544 2544
Size: 6820
StyI C'CwwG_G
Cuts at: 125 1334 1938 3086 3503 3638 3770 3952 4306
Size: 1209 604 1148 417 135 132 182 354
Cuts at: 4306 125
Size: 2639
Fragments arranged by size:
2639 1209 1148 604 417 354 182 135
132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 446 1856 2749 3051 4401 4534 4993 5204 446
Size: 1410 893 302 1350 133 459 211 2062
Fragments arranged by size:
2062 1410 1350 893 459 302 211 133
TatI w'GTAC_w
Cuts at: 905 6335 905
Size: 5430 1390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 964 996 2080 2112 3526 3558 6337 6369 964
Size: 32 1084 32 1414 32 2779 32 1415
Fragments arranged by size:
2779 1415 1414 1084 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3328 4615 3328
Size: 1287 5533
XbaI T'CTAG_A
Cuts at: 3849 3849
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1440 2631 4313 1440
Size: 1191 1682 3947
Fragments arranged by size:
3947 1682 1191
XhoI C'TCGA_G
Cuts at: 875 875
Size: 6820
XmaI C'CCGG_G
Cuts at: 3838 3838
Size: 6820
ZraI GAC'GTC
Cuts at: 2555 2555
Size: 6820
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PmlI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XmnI