Restriction map for
pF1KSDA0230
(Circular) (Six-base) MAPSORT of: pF1KSDA0230.seq Check: 9514 from: 1 to: 7576
pF1KSDA0230 7576 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 931 4468 931
Size: 3537 4039
AatII G_ACGT'C
Cuts at: 1973 3931 1973
Size: 1958 5618
AccI GT'mk_AC
Cuts at: 2213 3650 4612 2213
Size: 1437 962 5177
Fragments arranged by size:
5177 1437 962
Acc65I G'GTAC_C
Cuts at: 4590 4590
Size: 7576
AclI AA'CG_TT
Cuts at: 1961 7416 1961
Size: 5455 2121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 3318 4227 4263 5399 5831 6580 6848 1871
Size: 1447 909 36 1136 432 749 268 2599
Fragments arranged by size:
2599 1447 1136 909 749 432 268 36
AfeI AGC'GCT
Cuts at: 136 136
Size: 7576
AflIII A'CryG_T
Cuts at: 2327 2672 2867 3674 3986 4926 6032 7009 2327
Size: 345 195 807 312 940 1106 977 2894
Fragments arranged by size:
2894 1106 977 940 807 345 312 195
AgeI A'CCGG_T
Cuts at: 5964 5964
Size: 7576
AhdI GACnn_n'nnGTC
Cuts at: 2250 2250
Size: 7576
AleI CACnn'nnGTG
Cuts at: 262 1699 1729 2623 3973 262
Size: 1437 30 894 1350 3865
Fragments arranged by size:
3865 1437 1350 894 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 280 295 1288 4118 280
Size: 15 993 2830 3738
Fragments arranged by size:
3738 2830 993 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3538 3570 3538
Size: 32 7544
AlwNI CAG_nnn'CTG
Cuts at: 2265 2598 3528 4879 6448 2265
Size: 333 930 1351 1569 3393
Fragments arranged by size:
3393 1569 1351 930 333
ApaI G_GGCC'C
Cuts at: 148 2745 3814 148
Size: 2597 1069 3910
Fragments arranged by size:
3910 2597 1069
ApaLI G'TGCA_C
Cuts at: 6346 6346
Size: 7576
ApoI r'AATT_y
Cuts at: 456 1834 4578 6929 7185 456
Size: 1378 2744 2351 256 847
Fragments arranged by size:
2744 2351 1378 847 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7576
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7576
AvaI C'yCGr_G
Cuts at: 148 604 900 1780 2459 2726 3971 4594 148
Size: 456 296 880 679 267 1245 623 3130
Fragments arranged by size:
3130 1245 880 679 623 456 296 267
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1047 1080 1047
Size: 33 7543
BamHI G'GATC_C
Cuts at: 2121 3623 3941 4599 2121
Size: 1502 318 658 5098
Fragments arranged by size:
5098 1502 658 318
BanI G'GyrC_C
Cuts at: 152 306 1559 1741 2362 2395 3792 4590 5004
Size: 154 1253 182 621 33 1397 798 414
Cuts at: 5004 5252 5287 152
Size: 248 35 2441
Fragments arranged by size:
2441 1397 1253 798 621 414 248 182
154 35 33
BanII G_rGCy'C
Cuts at: 148 881 1781 2745 3025 3542 3814 4588 5618
Size: 733 900 964 280 517 272 774 1030
Cuts at: 5618 148
Size: 2106
Fragments arranged by size:
2106 1030 964 900 774 733 517 280
272
BbeI G_GCGC'C
Cuts at: 156 310 1745 2399 5008 5256 156
Size: 154 1435 654 2609 248 2476
Fragments arranged by size:
2609 2476 1435 654 248 154
BbsI GAAGACnn'nnnn_
Cuts at: 452 997 1111 3820 4082 4312 452
Size: 545 114 2709 262 230 3716
Fragments arranged by size:
3716 2709 545 262 230 114
BbvCI CC'TCA_GC
Cuts at: 379 4236 379
Size: 3857 3719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2267 2301 2739 2773 2267
Size: 34 438 34 7070
Fragments arranged by size:
7070 438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 419 2123 5472 6235 7576 419
Size: 1704 3349 763 1341 419
Fragments arranged by size:
3349 1704 1341 763 419
BclI T'GATC_A
Cuts at: 5094 5094
Size: 7576
BfrBI ATG'CAT
Cuts at: 276 4261 4423 276
Size: 3985 162 3429
Fragments arranged by size:
3985 3429 162
BglI GCCn_nnn'nGGC
Cuts at: 208 2924 3328 3809 208
Size: 2716 404 481 3975
Fragments arranged by size:
3975 2716 481 404
BglII A'GATC_T
Cuts at: 327 5089 7128 327
Size: 4762 2039 775
Fragments arranged by size:
4762 2039 775
BlpI GC'TnA_GC
Cuts at: 982 4686 982
Size: 3704 3872
Bme1580I G_kGCm'C
Cuts at: 148 299 2495 2745 3518 3814 5199 5292 6350
Size: 151 2196 250 773 296 1385 93 1058
Cuts at: 6350 148
Size: 1374
Fragments arranged by size:
2196 1385 1374 1058 773 296 250 151
93
BmgBI CAC'GTC
Cuts at: 3987 3987
Size: 7576
BmrI ACTGGGnnnn_n'
Cuts at: 1693 3159 4190 4786 4867 5202 7348 1693
Size: 1466 1031 596 81 335 2146 1921
Fragments arranged by size:
2146 1921 1466 1031 596 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5941 5941
Size: 7576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 307 1124 1228 2751 123
Size: 184 817 104 1523 4948
Fragments arranged by size:
4948 1523 817 184 104
Bpu10I CC'TnA_GC
Cuts at: 379 2632 4236 379
Size: 2253 1604 3719
Fragments arranged by size:
3719 2253 1604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3118 4159 4750 5629 6123 6421 3118
Size: 1041 591 879 494 298 4273
Fragments arranged by size:
4273 1041 879 591 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1142 2536 3169 4139 7280 1142
Size: 1394 633 970 3141 1438
Fragments arranged by size:
3141 1438 1394 970 633
BsaAI yAC'GTr
Cuts at: 4566 4927 4566
Size: 361 7215
BsaBI GATnn'nnATC
Cuts at: 6 5088 7127 6
Size: 5082 2039 455
Fragments arranged by size:
5082 2039 455
BsaHI Gr'CG_yC
Cuts at: 153 307 1438 1742 1970 2396 3928 5005 5253
Size: 154 1131 304 228 426 1532 1077 248
Cuts at: 5253 5957 7453 153
Size: 704 1496 276
Fragments arranged by size:
1532 1496 1131 1077 704 426 304 276
248 228 154
BsaWI w'CCGG_w
Cuts at: 629 2108 2348 3443 4763 5284 5964 6238 6385
Size: 1479 240 1095 1320 521 680 274 147
Cuts at: 6385 6796 629
Size: 411 1409
Fragments arranged by size:
1479 1409 1320 1095 680 521 411 274
240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2599 2629 3538 3568 2599
Size: 30 909 30 6607
Fragments arranged by size:
6607 909 30 30
BseYI C'CCAG_C
Cuts at: 1717 3069 3264 3807 3905 4999 5757 6336 6832
Size: 1352 195 543 98 1094 758 579 496
Cuts at: 6832 1717
Size: 2461
Fragments arranged by size:
2461 1352 1094 758 579 543 496 195
98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1233 1702 1771 1782 2697 2968 3997 532
Size: 701 469 69 11 915 271 1029 4111
Fragments arranged by size:
4111 1029 915 701 469 271 69 11
BsiEI CG_ry'CG
Cuts at: 104 5116 5162 6372 104
Size: 5012 46 1210 1308
Fragments arranged by size:
5012 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1363 1952 2881 3110 3542 4588 5366 5556 6350
Size: 589 929 229 432 1046 778 190 794
Cuts at: 6350 1363
Size: 2589
Fragments arranged by size:
2589 1046 929 794 778 589 432 229
190
BsmI GAATG_Cn'
Cuts at: 367 1633 1712 4444 367
Size: 1266 79 2732 3499
Fragments arranged by size:
3499 2732 1266 79
Bsp1286I G_dGCh'C
Cuts at: 148 299 881 1363 1781 1952 2495 2745 2881
Size: 151 582 482 418 171 543 250 136
Cuts at: 2881 3025 3110 3518 3542 3814 4588 5199 5292
Size: 144 85 408 24 272 774 611 93
Cuts at: 5292 5366 5556 5618 6350 148
Size: 74 190 62 732 1374
Fragments arranged by size:
1374 774 732 611 582 543 482 418
408 272 250 190 171 151 144 136
93 85 74 62 24
BspEI T'CCGG_A
Cuts at: 3443 6796 3443
Size: 3353 4223
BspHI T'CATG_A
Cuts at: 2705 2804 6752 2705
Size: 99 3948 3529
Fragments arranged by size:
3948 3529 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 554 931 1882 2384 4443 4468 4624 4838 5140
Size: 377 951 502 2059 25 156 214 302
Cuts at: 5140 5521 554
Size: 381 2609
Fragments arranged by size:
2609 2059 951 502 381 377 302 214
156 25
BsrBI CCG'CTC
Cuts at: 50 2738 3549 5866 5920 7406 7571 50
Size: 2688 811 2317 54 1486 165 55
Fragments arranged by size:
2688 2317 1486 811 165 55 54
BsrDI GCAATG_nn'
Cuts at: 394 2170 3116 3395 5486 394
Size: 1776 946 279 2091 2484
Fragments arranged by size:
2484 2091 1776 946 279
BsrFI r'CCGG_y
Cuts at: 1553 2303 3371 3803 5572 5753 5964 1553
Size: 750 1068 432 1769 181 211 3165
Fragments arranged by size:
3165 1769 1068 750 432 211 181
BsrGI T'GTAC_A
Cuts at: 476 2193 2483 476
Size: 1717 290 5569
Fragments arranged by size:
5569 1717 290
BssSI C'ACGA_G
Cuts at: 172 3775 5845 6205 172
Size: 3603 2070 360 1543
Fragments arranged by size:
3603 2070 1543 360
BstAPI GCAn_nnn'nTGC
Cuts at: 282 2675 4951 282
Size: 2393 2276 2907
Fragments arranged by size:
2907 2393 2276
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7576
BstEII G'GTnAC_C
Cuts at: 1116 1258 4455 1116
Size: 142 3197 4237
Fragments arranged by size:
4237 3197 142
BstXI CCAn_nnnn'nTGG
Cuts at: 2625 2625
Size: 7576
BstYI r'GATC_y
Cuts at: 1 327 353 488 1241 1986 2121 3200 3236
Size: 326 26 135 753 745 135 1079 36
Cuts at: 3236 3623 3713 3941 4034 4599 5066 5089 5424
Size: 387 90 228 93 565 467 23 335
Cuts at: 5424 5670 6684 6770 6782 7128 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1079 1014 753 745 565 467 449 387
346 335 326 246 228 135 135 93
90 86 36 26 23 12
Bsu36I CC'TnA_GG
Cuts at: 1123 1442 1123
Size: 319 7257
BtgI C'CryG_G
Cuts at: 125 203 302 1237 1285 1472 1547 2618 2821
Size: 78 99 935 48 187 75 1071 203
Cuts at: 2821 2922 2993 3122 3276 3326 3560 3618 4183
Size: 101 71 129 154 50 234 58 565
Cuts at: 4183 125
Size: 3518
Fragments arranged by size:
3518 1071 935 565 234 203 187 154
129 101 99 78 75 71 58 50
48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1500 2259 5524 5705 5868 1500
Size: 759 3265 181 163 3208
Fragments arranged by size:
3265 3208 759 181 163
BtsI GCAGTG_nn'
Cuts at: 708 2590 2661 3361 4817 708
Size: 1882 71 700 1456 3467
Fragments arranged by size:
3467 1882 1456 700 71
ClaI AT'CG_AT
Cuts at: 3467 3467
Size: 7576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1070 1105 1070
Size: 35 7541
DraI TTT'AAA
Cuts at: 461 4573 461
Size: 4112 3464
DraIII CAC_nnn'GTG
Cuts at: 2999 3095 3573 4367 4409 2999
Size: 96 478 794 42 6166
Fragments arranged by size:
6166 794 478 96 42
DrdI GACnn_nn'nnGTC
Cuts at: 3646 4453 5280 6140 3646
Size: 807 827 860 5082
Fragments arranged by size:
5082 860 827 807
EaeI y'GGCC_r
Cuts at: 128 200 782 1091 2912 3041 3342 3590 3935
Size: 72 582 309 1821 129 301 248 345
Cuts at: 3935 5159 5333 5724 5751 7098 128
Size: 1224 174 391 27 1347 606
Fragments arranged by size:
1821 1347 1224 606 582 391 345 309
301 248 174 129 72 27
EagI C'GGCC_G
Cuts at: 5159 5159
Size: 7576
EarI CTCTTCn'nnn_
Cuts at: 673 836 1424 4537 5597 5807 673
Size: 163 588 3113 1060 210 2442
Fragments arranged by size:
3113 2442 1060 588 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 751 4015 4564 5782 6094 6240 7386 751
Size: 3264 549 1218 312 146 1146 941
Fragments arranged by size:
3264 1218 1146 941 549 312 146
EcoICRI GAG'CTC
Cuts at: 3540 4586 3540
Size: 1046 6530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 307 1124 1228 1871 2751 3318 4227 4263
Size: 184 817 104 643 880 567 909 36
Cuts at: 4263 5399 5831 6580 6848 123
Size: 1136 432 749 268 851
Fragments arranged by size:
1136 909 880 851 817 749 643 567
432 268 184 104 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2635 3244 2635
Size: 609 6967
EcoO109I rG'GnC_Cy
Cuts at: 144 145 860 1025 2741 3684 3786 3811 4517
Size: 1 715 165 1716 943 102 25 706
Cuts at: 4517 4713 144
Size: 196 3007
Fragments arranged by size:
3007 1716 943 715 706 196 165 102
25 1
EcoRI G'AATT_C
Cuts at: 4578 4578
Size: 7576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5593 5593
Size: 7576
FspI TGC'GCA
Cuts at: 5355 5355
Size: 7576
HaeII r_GCGC'y
Cuts at: 138 156 172 310 1220 1745 2399 2421 3775
Size: 18 16 138 910 525 654 22 1354
Cuts at: 3775 3852 5008 5256 6280 7259 138
Size: 77 1156 248 1024 979 455
Fragments arranged by size:
1354 1156 1024 979 910 654 525 455
248 138 77 22 18 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 645 677 883 915 2236 2268 2599
Size: 32 135 32 206 32 1321 32 331
Cuts at: 2599 2631 5416 5446 5448 5478 478
Size: 32 2785 30 2 30 2576
Fragments arranged by size:
2785 2576 1321 331 206 135 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1281 1641 2031 2214 3357 4613 1281
Size: 360 390 183 1143 1256 4244
Fragments arranged by size:
4244 1256 1143 390 360 183
HindIII A'AGCT_T
Cuts at: 3425 4472 3425
Size: 1047 6529
KasI G'GCGC_C
Cuts at: 152 306 1741 2395 5004 5252 152
Size: 154 1435 654 2609 248 2476
Fragments arranged by size:
2609 2476 1435 654 248 154
KpnI G_GTAC'C
Cuts at: 4594 4594
Size: 7576
MfeI C'AATT_G
Cuts at: 497 497
Size: 7576
MluI A'CGCG_T
Cuts at: 7009 7009
Size: 7576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 381 2675 2888 6247 6431 381
Size: 2294 213 3359 184 1526
Fragments arranged by size:
3359 2294 1526 213 184
MscI TGG'CCA
Cuts at: 130 2914 3344 3937 5335 130
Size: 2784 430 593 1398 2371
Fragments arranged by size:
2784 2371 1398 593 430
MslI CAynn'nnrTG
Cuts at: 262 1699 1729 2572 2578 2623 3820 3973 5563
Size: 1437 30 843 6 45 1197 153 1590
Cuts at: 5563 5690 262
Size: 127 2148
Fragments arranged by size:
2148 1590 1437 1197 843 153 127 45
30 6
MspA1I CmG'CkG
Cuts at: 158 242 781 1239 1287 1346 2747 2786 2823
Size: 84 539 458 48 59 1401 39 37
Cuts at: 2823 2924 2952 2967 3069 3328 3525 3606 3909
Size: 101 28 15 102 259 197 81 303
Cuts at: 3909 4025 4685 4999 5359 6374 6619 158
Size: 116 660 314 360 1015 245 1115
Fragments arranged by size:
1401 1115 1015 660 539 458 360 314
303 259 245 197 116 102 101 84
81 59 48 39 37 28 15
NaeI GCC'GGC
Cuts at: 3805 5755 3805
Size: 1950 5626
NarI GG'CG_CC
Cuts at: 153 307 1742 2396 5005 5253 153
Size: 154 1435 654 2609 248 2476
Fragments arranged by size:
2609 2476 1435 654 248 154
NcoI C'CATG_G
Cuts at: 125 3560 125
Size: 3435 4141
NgoMIV G'CCGG_C
Cuts at: 3803 5753 3803
Size: 1950 5626
NruI TCG'CGA
Cuts at: 5937 5937
Size: 7576
NsiI A_TGCA'T
Cuts at: 278 4263 4425 278
Size: 3985 162 3429
Fragments arranged by size:
3985 3429 162
NspI r_CATG'y
Cuts at: 276 2194 2331 2577 2754 3006 3087 4427 4627
Size: 1918 137 246 177 252 81 1340 200
Cuts at: 4627 5127 6036 276
Size: 500 909 1816
Fragments arranged by size:
1918 1816 1340 909 500 252 246 200
177 137 81
PasI CC'CwG_GG
Cuts at: 1770 3886 4040 4513 1770
Size: 2116 154 473 4833
Fragments arranged by size:
4833 2116 473 154
PciI A'CATG_T
Cuts at: 2327 6032 2327
Size: 3705 3871
PflMI CCAn_nnn'nTGG
Cuts at: 1077 1473 1796 2598 1077
Size: 396 323 802 6055
Fragments arranged by size:
6055 802 396 323
PfoI T'CCnGG_A
Cuts at: 1246 2597 2956 3716 1246
Size: 1351 359 760 5106
Fragments arranged by size:
5106 1351 760 359
PmeI GTTT'AAAC
Cuts at: 4573 4573
Size: 7576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2305 2337 3538 3570 4274 4306 6715 6747 2305
Size: 32 1201 32 704 32 2409 32 3134
Fragments arranged by size:
3134 2409 1201 704 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1025 3684 3786 1025
Size: 2659 102 4815
Fragments arranged by size:
4815 2659 102
PshAI GACnn'nnGTC
Cuts at: 1892 1973 2072 1892
Size: 81 99 7396
Fragments arranged by size:
7396 99 81
PspOMI G'GGCC_C
Cuts at: 144 2741 3810 144
Size: 2597 1069 3910
Fragments arranged by size:
3910 2597 1069
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 898 930 2800 2832 898
Size: 32 1870 32 5642
Fragments arranged by size:
5642 1870 32 32
PstI C_TGCA'G
Cuts at: 928 1887 2381 2668 4621 5306 928
Size: 959 494 287 1953 685 3198
Fragments arranged by size:
3198 1953 959 685 494 287
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7576
PvuII CAG'CTG
Cuts at: 3069 3525 3909 4025 4999 5359 3069
Size: 456 384 116 974 360 5286
Fragments arranged by size:
5286 974 456 384 360 116
RsrII CG'GwC_CG
Cuts at: 2954 5769 2954
Size: 2815 4761
SacI G_AGCT'C
Cuts at: 3542 4588 3542
Size: 1046 6530
SacII CC_GC'GG
Cuts at: 1240 1288 2824 2925 3329 1240
Size: 48 1536 101 404 5487
Fragments arranged by size:
5487 1536 404 101 48
SalI G'TCGA_C
Cuts at: 2212 4611 2212
Size: 2399 5177
SanDI GG'GwC_CC
Cuts at: 1025 1025
Size: 7576
SapI GCTCTTCn'nnn_
Cuts at: 836 1424 5597 5807 836
Size: 588 4173 210 2605
Fragments arranged by size:
4173 2605 588 210
SbfI CC_TGCA'GG
Cuts at: 4621 4621
Size: 7576
ScaI AGT'ACT
Cuts at: 7093 7093
Size: 7576
SexAI A'CCwGG_T
Cuts at: 1562 2246 3788 1562
Size: 684 1542 5350
Fragments arranged by size:
5350 1542 684
SfcI C'TryA_G
Cuts at: 33 924 1883 2377 2664 3642 4080 4617 5302
Size: 891 959 494 287 978 438 537 685
Cuts at: 5302 6297 6488 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 978 959 891 685 537 494
438 287 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 208 208
Size: 7576
SfoI GGC'GCC
Cuts at: 154 308 1743 2397 5006 5254 154
Size: 154 1435 654 2609 248 2476
Fragments arranged by size:
2609 2476 1435 654 248 154
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7576
SgrAI Cr'CCGG_yG
Cuts at: 2303 2303
Size: 7576
SmaI CCC'GGG
Cuts at: 150 2728 3973 4596 150
Size: 2578 1245 623 3130
Fragments arranged by size:
3130 2578 1245 623
SmlI C'TyrA_G
Cuts at: 604 3133 4138 4729 5644 6138 6400 604
Size: 2529 1005 591 915 494 262 1780
Fragments arranged by size:
2529 1780 1005 915 591 494 262
SnaBI TAC'GTA
Cuts at: 4566 4566
Size: 7576
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7576
SphI G_CATG'C
Cuts at: 276 3006 3087 4427 4627 5127 276
Size: 2730 81 1340 200 500 2725
Fragments arranged by size:
2730 2725 1340 500 200 81
StyI C'CwwG_G
Cuts at: 125 1493 1526 3560 4708 5062 125
Size: 1368 33 2034 1148 354 2639
Fragments arranged by size:
2639 2034 1368 1148 354 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2584 3181 5157 5290 5749 5960 2584
Size: 597 1976 133 459 211 4200
Fragments arranged by size:
4200 1976 597 459 211 133
TatI w'GTAC_w
Cuts at: 476 914 2193 2483 7091 476
Size: 438 1279 290 4608 961
Fragments arranged by size:
4608 1279 961 438 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 417 449 806 838 1242 1274 1468 1500 3495
Size: 32 357 32 404 32 194 32 1995
Cuts at: 3495 3527 3856 3888 7093 7125 417
Size: 32 329 32 3205 32 868
Fragments arranged by size:
3205 1995 868 404 357 329 194 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1384 1450 5371 1384
Size: 66 3921 3589
Fragments arranged by size:
3921 3589 66
XbaI T'CTAG_A
Cuts at: 531 4605 531
Size: 4074 3502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 2511 3066 3186 4368 5069 1793
Size: 718 555 120 1182 701 4300
Fragments arranged by size:
4300 1182 718 701 555 120
XhoI C'TCGA_G
Cuts at: 604 604
Size: 7576
XmaI C'CCGG_G
Cuts at: 148 2726 3971 4594 148
Size: 2578 1245 623 3130
Fragments arranged by size:
3130 2578 1245 623
XmnI GAAnn'nnTTC
Cuts at: 2446 2446
Size: 7576
ZraI GAC'GTC
Cuts at: 1971 3929 1971
Size: 1958 5618
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BmtI BseRI BsiWI BsmBI BssHII
BstZ17I EcoRV FseI FspAI HpaI NdeI NheI NotI
PacI PmlI PsiI PspXI SrfI SspI StuI SwaI